AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029086


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029086 MtrunA17_Chr5g0440591 91.964 336 6 1 172 507 301 615 0.0 641
MsaG029086 MtrunA17_Chr5g0440591 73.810 252 49 4 1 243 1 244 2.43e-117 358
MsaG029086 MtrunA17_Chr3g0118361 48.895 362 119 18 172 505 304 627 4.99e-87 280
MsaG029086 MtrunA17_Chr3g0118361 72.000 200 50 4 1 195 2 200 4.01e-80 262
MsaG029086 MtrunA17_Chr2g0329151 69.600 125 36 1 36 158 25 149 6.15e-55 194
MsaG029086 MtrunA17_Chr3g0124291 60.000 110 41 1 46 155 29 135 4.37e-39 147
MsaG029086 MtrunA17_Chr8g0382561 57.627 118 45 2 39 155 12 125 1.72e-37 143
MsaG029086 MtrunA17_Chr6g0476301 65.079 63 22 0 103 165 1 63 6.55e-24 103
MsaG029086 MtrunA17_Chr6g0473191 65.079 63 22 0 103 165 1 63 1.50e-23 100
MsaG029086 MtrunA17_Chr6g0476291 61.905 63 24 0 103 165 1 63 7.42e-23 98.2
MsaG029086 MtrunA17_Chr7g0260281 53.846 65 30 0 92 156 1 65 8.80e-19 82.4
MsaG029086 MtrunA17_Chr3g0080401 53.968 63 29 0 103 165 1 63 3.19e-18 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029086 AT3G20770.1 61.508 252 79 5 2 243 1 244 9.78e-92 293
MsaG029086 AT3G20770.1 39.601 351 171 15 172 505 301 627 4.24e-55 195
MsaG029086 AT2G27050.1 74.566 173 41 3 1 171 1 172 2.39e-80 261
MsaG029086 AT2G27050.1 38.109 349 133 16 172 505 303 583 2.49e-42 159
MsaG029086 AT1G73730.2 64.493 138 43 2 34 167 11 146 5.03e-53 188
MsaG029086 AT1G73730.1 64.493 138 43 2 34 167 23 158 7.67e-53 188
MsaG029086 AT5G10120.1 51.938 129 58 3 28 155 4 129 4.34e-37 143
MsaG029086 AT5G21120.2 57.143 140 54 2 22 158 31 167 5.73e-35 137
MsaG029086 AT5G21120.3 57.143 140 54 2 22 158 24 160 5.95e-35 137
MsaG029086 AT5G21120.1 57.143 140 54 2 22 158 24 160 5.95e-35 137
MsaG029086 AT5G65100.1 48.438 128 49 2 44 155 33 159 2.87e-32 130

Find 141 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGCACAAGGCTTTGTTTA+TGG 0.190580 5:-72757464 MsaT029086.1:CDS
ACCTCGGTAGCATGAAAATT+AGG 0.199653 5:+72756468 None:intergenic
CCACCTTCATTCGATTTAAC+TGG 0.245739 5:-72756378 MsaT029086.1:CDS
TTTAACTGGGTTTGGAGTTT+CGG 0.307024 5:-72756364 MsaT029086.1:CDS
AAGCTTTGTGGGAAAATAAC+TGG 0.313704 5:+72756441 None:intergenic
GCAGGCTGAACTGTAGAATT+AGG 0.320347 5:+72756411 None:intergenic
ATCTGCTTGATACTTGGATA+TGG 0.326260 5:+72757343 None:intergenic
AGGCACAAGGCTTTGTTTAT+GGG 0.329778 5:-72757463 MsaT029086.1:CDS
TGCATTATCTGCTTGATACT+TGG 0.339703 5:+72757337 None:intergenic
GTATGGTTCTTCATGTGATT+TGG 0.348025 5:-72756001 MsaT029086.1:CDS
TCATCCTCGACCACCGAATC+TGG 0.353514 5:+72757698 None:intergenic
TACCTCCGCCAGAGGATAAC+GGG 0.362721 5:+72756871 None:intergenic
AACAACAAGATGTTTCAATA+TGG 0.364077 5:-72755915 MsaT029086.1:CDS
TGATGAGAAGGTTGCTGAAC+TGG 0.365013 5:+72756708 None:intergenic
CCTTTCAAGATAGGTCTTCC+AGG 0.366977 5:-72756551 MsaT029086.1:CDS
ATTATTGTTGTTGTTGAGGT+TGG 0.371583 5:+72756035 None:intergenic
GTCCTGTTGTTGCTGAATGA+TGG 0.379003 5:+72756158 None:intergenic
AATCTTCATCCATCCAATCT+TGG 0.380733 5:-72756756 MsaT029086.1:CDS
ATCAATTCCTTCTTTAGCCT+TGG 0.381143 5:+72757589 None:intergenic
TCATTCGATTTAACTGGGTT+TGG 0.382118 5:-72756372 MsaT029086.1:CDS
TTATCCTCTGGCGGAGGTAC+TGG 0.383389 5:-72756867 MsaT029086.1:CDS
TTCAATAACTACACAATTGG+TGG 0.384657 5:+72756266 None:intergenic
GGTTTCAAAAGGAGAGTTCA+TGG 0.396115 5:+72756056 None:intergenic
CAAGCAGATAATGCAATTCC+AGG 0.406735 5:-72757329 MsaT029086.1:CDS
CATTCTATCCATCCCAAGAT+TGG 0.409448 5:+72756743 None:intergenic
TTGTGGTACACTTGGGGAAG+GGG 0.411806 5:-72757747 MsaT029086.1:CDS
TAGGTTGAACAAAGCTTTGT+GGG 0.411860 5:+72756430 None:intergenic
AGAAACAGTCCTGCAGGTTA+TGG 0.412726 5:-72756495 MsaT029086.1:CDS
CTCGCCTTGCCTTTCAAGAT+AGG 0.415938 5:-72756560 MsaT029086.1:CDS
TGGCGGAGGTACTGGTTCTA+TGG 0.421852 5:-72756859 MsaT029086.1:CDS
AATAATATGCATCCCATGTA+TGG 0.430283 5:-72756018 MsaT029086.1:CDS
TCCTGTTGTTGCTGAATGAT+GGG 0.434795 5:+72756159 None:intergenic
GGCAAGGCAGTCTCAAGAAC+AGG 0.441318 5:-72757558 MsaT029086.1:CDS
ATCTTCATCCATCCAATCTT+GGG 0.443460 5:-72756755 MsaT029086.1:CDS
TTGTTGAGGTTGGTTTCAAA+AGG 0.451254 5:+72756045 None:intergenic
GGGAGAGGTTGTCACAAACT+TGG 0.451465 5:-72756679 MsaT029086.1:CDS
CCTCGGTAGCATGAAAATTA+GGG 0.456819 5:+72756469 None:intergenic
CACCTTCATTCGATTTAACT+GGG 0.462918 5:-72756377 MsaT029086.1:CDS
GGCTATGATACACATGTTAT+AGG 0.462962 5:-72756306 MsaT029086.1:CDS
TCACAAACTTGGATTTCATT+CGG 0.464079 5:-72756668 MsaT029086.1:CDS
TGATTATAGTGATGAAGAAA+TGG 0.464489 5:-72757678 MsaT029086.1:CDS
TTTGTGGTACACTTGGGGAA+GGG 0.466355 5:-72757748 MsaT029086.1:CDS
GCCCATCATTCAGCAACAAC+AGG 0.469177 5:-72756160 MsaT029086.1:CDS
GCATATTATTGTTGTTGTTG+AGG 0.474406 5:+72756031 None:intergenic
CAGGCAAACCGAGCCAGATT+CGG 0.479153 5:-72757711 MsaT029086.1:CDS
TCGTGAATGGTGGAAGGATA+AGG 0.479265 5:-72757393 MsaT029086.1:CDS
GAGAGTTCATGGCCTTGAAC+CGG 0.490862 5:+72756067 None:intergenic
AAGGGGATATTTCTTCTGTC+AGG 0.493214 5:-72757730 MsaT029086.1:CDS
ACGAAGATTGTCAGATGCTC+CGG 0.495666 5:+72757412 None:intergenic
AATTCCCGAGAAGGGAAAAC+CGG 0.496336 5:-72757438 MsaT029086.1:CDS
TTGTCAGATGCTCCGGTCAC+CGG 0.496468 5:+72757419 None:intergenic
GCAACCTTCTCATCAAATCA+AGG 0.497251 5:-72756700 MsaT029086.1:CDS
AAATCAGTTTGACCGGTTCA+AGG 0.500225 5:-72756079 MsaT029086.1:CDS
GATGATGATGTTTGAGGACA+TGG 0.505605 5:-72757795 MsaT029086.1:CDS
TGCAGTGAGTATGATGTTGA+TGG 0.508204 5:-72756825 MsaT029086.1:CDS
GTACCTCCGCCAGAGGATAA+CGG 0.514038 5:+72756870 None:intergenic
TGATACTTGGATATGGCAGC+TGG 0.514747 5:+72757350 None:intergenic
ATGATCAGCGACCTTATGTC+AGG 0.516438 5:-72756327 MsaT029086.1:CDS
CGAGCCAGATTCGGTGGTCG+AGG 0.516671 5:-72757702 MsaT029086.1:CDS
GCAATTCCAGGGAAGAATGA+TGG 0.516754 5:-72757317 MsaT029086.1:CDS
CTCGACCACCGAATCTGGCT+CGG 0.517344 5:+72757703 None:intergenic
TAACGACTTCAACATGATGA+TGG 0.518203 5:-72756631 MsaT029086.1:CDS
TTGCATCCATCATTCTTCCC+TGG 0.518960 5:+72757311 None:intergenic
ATGAGCTTGAGCGGAGGATG+TGG 0.520497 5:-72757649 MsaT029086.1:CDS
ATCACATGAAGAACCATACA+TGG 0.521967 5:+72756005 None:intergenic
GTGTATCATAGCCTGACATA+AGG 0.531266 5:+72756316 None:intergenic
TCTCCCTTGATTTGATGAGA+AGG 0.537373 5:+72756696 None:intergenic
ATTCAATTGATGATTGTCCC+TGG 0.537403 5:+72756533 None:intergenic
ATCTTGGGATGGATAGAATG+AGG 0.537865 5:-72756740 MsaT029086.1:CDS
TAGATCCTCCTTAAAATCAA+AGG 0.539849 5:+72755975 None:intergenic
ACTTGGATTTCATTCGGAAG+AGG 0.540565 5:-72756662 MsaT029086.1:CDS
TTAGGTTGAACAAAGCTTTG+TGG 0.541625 5:+72756429 None:intergenic
GGGTTTGGAGTTTCGGAGGA+CGG 0.542268 5:-72756357 MsaT029086.1:CDS
GAACAAACCAAGGCTAAAGA+AGG 0.542936 5:-72757596 MsaT029086.1:CDS
TTTGAATCAAGGAATGGTGA+TGG 0.544516 5:-72756127 MsaT029086.1:CDS
GAAGTACATGCTGAAAATGA+TGG 0.545558 5:-72757495 MsaT029086.1:CDS
AGCTTGAGCGGAGGATGTGG+AGG 0.545764 5:-72757646 MsaT029086.1:CDS
GAGAAGGGAAAACCGGTGAC+CGG 0.547110 5:-72757431 MsaT029086.1:CDS
AGAAACAGAAACAGTCCTGC+AGG 0.548675 5:-72756501 MsaT029086.1:CDS
GGACTGTTTCTGTTTCTATG+TGG 0.550312 5:+72756507 None:intergenic
GAAGGTGGAACTAAACTAGC+AGG 0.551330 5:+72756393 None:intergenic
AGGATAAGGTTCGGTTCGAT+AGG 0.552012 5:-72757379 MsaT029086.1:CDS
TTAGGGTCGCCATAACCTGC+AGG 0.552645 5:+72756486 None:intergenic
GCTGAATGATGGGCTGAGAA+AGG 0.553081 5:+72756169 None:intergenic
GCTGAATGATGGGCTGAGAA+AGG 0.553081 5:+72756202 None:intergenic
GCTGAATGATGGGCTGAGAA+AGG 0.553081 5:+72756235 None:intergenic
TTCTCATCAAATCAAGGGAG+AGG 0.554659 5:-72756694 MsaT029086.1:CDS
CTTTCAAGATAGGTCTTCCA+GGG 0.555713 5:-72756550 MsaT029086.1:CDS
TATGGGATAATTCCCGAGAA+GGG 0.556990 5:-72757446 MsaT029086.1:CDS
GGAAAAGATGATGATGTTTG+AGG 0.557260 5:-72757801 None:intergenic
CTATCCATCCCAAGATTGGA+TGG 0.557944 5:+72756747 None:intergenic
CTGACAATCTTCGTGAATGG+TGG 0.558385 5:-72757403 MsaT029086.1:CDS
CCTGGAAGACCTATCTTGAA+AGG 0.560673 5:+72756551 None:intergenic
CTACAACTACAAGGAGGAGT+AGG 0.563021 5:-72755955 MsaT029086.1:CDS
AACCCAGTTAAATCGAATGA+AGG 0.563631 5:+72756375 None:intergenic
GCGAGGAAAATCAGTTTGAC+CGG 0.565875 5:-72756086 MsaT029086.1:CDS
CATCTGACAATCTTCGTGAA+TGG 0.567255 5:-72757406 MsaT029086.1:CDS
TTATGGGATAATTCCCGAGA+AGG 0.572124 5:-72757447 MsaT029086.1:CDS
AATGGTGGAAGGATAAGGTT+CGG 0.572803 5:-72757388 MsaT029086.1:CDS
AGAAGGAATTGATGCTGCAA+AGG 0.574701 5:-72757579 MsaT029086.1:CDS
GCAGTGAGTATGATGTTGAT+GGG 0.574936 5:-72756824 MsaT029086.1:CDS
AACTGGGTTTGGAGTTTCGG+AGG 0.578482 5:-72756361 MsaT029086.1:CDS
CGAGTCAAACTTTGATGTGG+AGG 0.584361 5:-72756793 MsaT029086.1:CDS
TAGTGATGAAGAAATGGATG+TGG 0.587114 5:-72757672 MsaT029086.1:CDS
GAAAATGATGGAGGTTTGCA+AGG 0.590144 5:-72757483 MsaT029086.1:CDS
ATGGAGGTTTGCAAGGCACA+AGG 0.590635 5:-72757476 MsaT029086.1:CDS
TCTTGGGATGGATAGAATGA+GGG 0.594489 5:-72756739 MsaT029086.1:CDS
CAACCTTCTCATCAAATCAA+GGG 0.596906 5:-72756699 MsaT029086.1:CDS
TCATCCATCCAATCTTGGGA+TGG 0.598664 5:-72756751 MsaT029086.1:CDS
CCAGTTAAATCGAATGAAGG+TGG 0.598931 5:+72756378 None:intergenic
CTTGGGATGGATAGAATGAG+GGG 0.601634 5:-72756738 MsaT029086.1:CDS
CAATCTTCGTGAATGGTGGA+AGG 0.601878 5:-72757399 MsaT029086.1:CDS
AAGCAGATAATGCAATTCCA+GGG 0.603704 5:-72757328 MsaT029086.1:CDS
GGCAGTCTCAAGAACAGGCG+AGG 0.607320 5:-72757553 MsaT029086.1:CDS
AAGGAGGATCTACAACTACA+AGG 0.608788 5:-72755964 MsaT029086.1:CDS
AAGGTTCGGTTCGATAGGAA+TGG 0.610184 5:-72757374 MsaT029086.1:CDS
GCAAACCGAGCCAGATTCGG+TGG 0.610299 5:-72757708 MsaT029086.1:CDS
ACTACAAGGAGGAGTAGGAA+TGG 0.611304 5:-72755950 MsaT029086.1:CDS
AAGATGTCAAGAGCTCAAGA+TGG 0.612194 5:-72757524 MsaT029086.1:CDS
GCTTCGCTGTAAGCGCATTG+AGG 0.613195 5:+72756582 None:intergenic
CGGCGAGTCAAACTTTGATG+TGG 0.614982 5:-72756796 MsaT029086.1:CDS
AAGACCTATCTTGAAAGGCA+AGG 0.620631 5:+72756556 None:intergenic
TATGATGTTGATGGGGCTGA+CGG 0.622733 5:-72756816 MsaT029086.1:CDS
GAATTGATGCTGCAAAGGCA+AGG 0.624873 5:-72757574 MsaT029086.1:CDS
GAATCAAGGAATGGTGATGG+AGG 0.630262 5:-72756124 MsaT029086.1:CDS
ACCTCCGCCAGAGGATAACG+GGG 0.630408 5:+72756872 None:intergenic
AATGATGGATGCAATTCCAT+CGG 0.635466 5:-72757302 MsaT029086.1:intron
GAAAATAACTGGCTTAACCT+CGG 0.643010 5:+72756452 None:intergenic
AGTCTCAAGAACAGGCGAGG+AGG 0.643483 5:-72757550 MsaT029086.1:CDS
GCTTGAGCGGAGGATGTGGA+GGG 0.653404 5:-72757645 MsaT029086.1:CDS
TGGATGTGGATGAGCTTGAG+CGG 0.660750 5:-72757658 MsaT029086.1:CDS
ATGATGATGTTTGAGGACAT+GGG 0.668420 5:-72757794 MsaT029086.1:CDS
AGAACCAGTACCTCCGCCAG+AGG 0.682787 5:+72756863 None:intergenic
TCACATGAAGAACCATACAT+GGG 0.685805 5:+72756006 None:intergenic
CTCCGCCAGAGGATAACGGG+GGG 0.685928 5:+72756874 None:intergenic
GCGACTGAAAGAACAAACCA+AGG 0.688069 5:-72757606 MsaT029086.1:CDS
CAGTGAGTATGATGTTGATG+GGG 0.693933 5:-72756823 MsaT029086.1:CDS
CCTCCGCCAGAGGATAACGG+GGG 0.696205 5:+72756873 None:intergenic
ATGTGGATGAGCTTGAGCGG+AGG 0.710465 5:-72757655 MsaT029086.1:CDS
GTACATGCTGAAAATGATGG+AGG 0.724912 5:-72757492 MsaT029086.1:CDS
GAGGATCTACAACTACAAGG+AGG 0.776973 5:-72755961 MsaT029086.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 72755906 72757816 72755906 ID=MsaG029086
Chr5 mRNA 72755906 72757816 72755906 ID=MsaT029086.1;Parent=MsaG029086
Chr5 exon 72755906 72756915 72755906 ID=MsaT029086.1.exon2;Parent=MsaT029086.1
Chr5 CDS 72755906 72756915 72755906 ID=cds.MsaT029086.1;Parent=MsaT029086.1
Chr5 exon 72757303 72757816 72757303 ID=MsaT029086.1.exon1;Parent=MsaT029086.1
Chr5 CDS 72757303 72757816 72757303 ID=cds.MsaT029086.1;Parent=MsaT029086.1
Gene Sequence

>MsaT029086.1

ATGATGATGTTTGAGGACATGGGATTTTGTGGTGATTTGGATGTGTTTTGTGGTACACTTGGGGAAGGGGATATTTCTTCTGTCAGGCAAACCGAGCCAGATTCGGTGGTCGAGGATGATTATAGTGATGAAGAAATGGATGTGGATGAGCTTGAGCGGAGGATGTGGAGGGACAAAATGCGTCTCAAGCGACTGAAAGAACAAACCAAGGCTAAAGAAGGAATTGATGCTGCAAAGGCAAGGCAGTCTCAAGAACAGGCGAGGAGGAAAAAGATGTCAAGAGCTCAAGATGGAATATTGAAGTACATGCTGAAAATGATGGAGGTTTGCAAGGCACAAGGCTTTGTTTATGGGATAATTCCCGAGAAGGGAAAACCGGTGACCGGAGCATCTGACAATCTTCGTGAATGGTGGAAGGATAAGGTTCGGTTCGATAGGAATGGTCCAGCTGCCATATCCAAGTATCAAGCAGATAATGCAATTCCAGGGAAGAATGATGGATGCAATTCCATCGAACTTTATCCAGATTATTGCCCCCCGTTATCCTCTGGCGGAGGTACTGGTTCTATGGTCATCAACGATTGCAGTGAGTATGATGTTGATGGGGCTGACGGCGAGTCAAACTTTGATGTGGAGGACCGAAAACCCGAAAATCTTCATCCATCCAATCTTGGGATGGATAGAATGAGGGGAAGCTTTCCAGTTCAGCAACCTTCTCATCAAATCAAGGGAGAGGTTGTCACAAACTTGGATTTCATTCGGAAGAGGAAGATCTCTAACGACTTCAACATGATGATGGAACCGAAAATGTACACATGCGAGCATCCTCAATGCGCTTACAGCGAAGCTCGCCTTGCCTTTCAAGATAGGTCTTCCAGGGACAATCATCAATTGAATTGTCCACATAGAAACAGAAACAGTCCTGCAGGTTATGGCGACCCTAATTTTCATGCTACCGAGGTTAAGCCAGTTATTTTCCCACAAAGCTTTGTTCAACCTAATTCTACAGTTCAGCCTGCTAGTTTAGTTCCACCTTCATTCGATTTAACTGGGTTTGGAGTTTCGGAGGACGGCCAGAAAATGATCAGCGACCTTATGTCAGGCTATGATACACATGTTATAGGAAACAACAATGCAAGTTCCACCAATTGTGTAGTTATTGAAAATCAAAACCTTTCTCAGCCCATCATTCAGCAACAACAAAACCTTTCTCAGCCCATCATTCAGCAACAACAAAACCTTTCTCAGCCCATCATTCAGCAACAACAGGACAATTTCTTTTTGAATCAAGGAATGGTGATGGAGGCAAACTTTTTTACTCGCGAGGAAAATCAGTTTGACCGGTTCAAGGCCATGAACTCTCCTTTTGAAACCAACCTCAACAACAACAATAATATGCATCCCATGTATGGTTCTTCATGTGATTTGGCATCCTTTGATTTTAAGGAGGATCTACAACTACAAGGAGGAGTAGGAATGGATGCACTTTACAAACAACAAGATGTTTCAATATGGAACTACTGA

Protein sequence

>MsaT029086.1

MMMFEDMGFCGDLDVFCGTLGEGDISSVRQTEPDSVVEDDYSDEEMDVDELERRMWRDKMRLKRLKEQTKAKEGIDAAKARQSQEQARRKKMSRAQDGILKYMLKMMEVCKAQGFVYGIIPEKGKPVTGASDNLREWWKDKVRFDRNGPAAISKYQADNAIPGKNDGCNSIELYPDYCPPLSSGGGTGSMVINDCSEYDVDGADGESNFDVEDRKPENLHPSNLGMDRMRGSFPVQQPSHQIKGEVVTNLDFIRKRKISNDFNMMMEPKMYTCEHPQCAYSEARLAFQDRSSRDNHQLNCPHRNRNSPAGYGDPNFHATEVKPVIFPQSFVQPNSTVQPASLVPPSFDLTGFGVSEDGQKMISDLMSGYDTHVIGNNNASSTNCVVIENQNLSQPIIQQQQNLSQPIIQQQQNLSQPIIQQQQDNFFLNQGMVMEANFFTREENQFDRFKAMNSPFETNLNNNNNMHPMYGSSCDLASFDFKEDLQLQGGVGMDALYKQQDVSIWNY*