AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029162


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029162 MtrunA17_Chr5g0441241 80.220 273 31 4 18 267 1 273 2.38e-122 350
MsaG029162 MtrunA17_Chr3g0081161 45.270 296 118 7 1 268 1 280 1.78e-69 216
MsaG029162 MtrunA17_Chr8g0354151 48.299 294 126 7 1 268 1 294 9.98e-69 215
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029162 AT3G12480.1 69.466 131 34 1 1 125 1 131 3.63e-59 190
MsaG029162 AT5G19490.1 76.364 110 24 1 1 110 1 108 6.89e-56 181

Find 56 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTAACTTCCTCGATTTC+TGG 0.151578 5:-73811223 None:intergenic
AACTAGCCAACTTAAGTAAA+AGG 0.241392 5:+73811513 MsaT029162.1:CDS
GTGAGCAAAGTTCCTGATTT+AGG 0.300821 5:+73810351 MsaT029162.1:CDS
ATGCAAGCTGATGAAGATAT+AGG 0.348420 5:+73809089 MsaT029162.1:CDS
GCTTAACTTCCTCGATTTCT+GGG 0.371274 5:-73811222 None:intergenic
ACTTAAGTTGGCTAGTTGTA+AGG 0.372538 5:-73811507 None:intergenic
AGGGGTAGAGGCAGGGGACG+TGG 0.387631 5:+73811088 MsaT029162.1:CDS
TAAAAGGATTGATGAAGATA+TGG 0.395167 5:+73811529 MsaT029162.1:CDS
GTGGAAGGCGGTAGGGGTAG+AGG 0.397290 5:+73811076 MsaT029162.1:CDS
GGCGGTAGGGGTAGAGGCAG+GGG 0.401288 5:+73811082 MsaT029162.1:CDS
TGCAAGCTGATGAAGATATA+GGG 0.408049 5:+73809090 MsaT029162.1:CDS
AAGGCGGTAGGGGTAGAGGC+AGG 0.420263 5:+73811080 MsaT029162.1:CDS
AGGGGACGTGGCCGCGGCCA+AGG 0.424123 5:+73811100 MsaT029162.1:CDS
AGAGGCAGGGGACGTGGCCG+CGG 0.424913 5:+73811094 MsaT029162.1:CDS
ATTTCGATTTAAATCTGAAT+CGG 0.427921 5:+73811322 MsaT029162.1:CDS
GCAGCAGAACCGCCTAAATC+AGG 0.433277 5:-73810363 None:intergenic
GAACAATGCTTAACTTCCTC+AGG 0.444484 5:-73811265 None:intergenic
ACTTCCTCAGGTTCTTTGAC+TGG 0.454445 5:-73811253 None:intergenic
TTAGGCGGTTCTGCTGCAAC+TGG 0.479475 5:+73810369 MsaT029162.1:CDS
CTTAAGTTGGCTAGTTGTAA+GGG 0.481167 5:-73811506 None:intergenic
ATTTAAATCTGAATCGGATC+CGG 0.484334 5:+73811328 MsaT029162.1:CDS
ATTTCATCCATGTCGTCATC+CGG 0.486479 5:-73811347 None:intergenic
TTACAAATGAAAAGCATTCA+CGG 0.491312 5:-73809345 None:intergenic
GAAGATGAACCGGATGGGAT+AGG 0.503037 5:-73811383 None:intergenic
AGGCGGTAGGGGTAGAGGCA+GGG 0.507667 5:+73811081 MsaT029162.1:CDS
ATCGGATCCGGATGACGACA+TGG 0.543974 5:+73811340 MsaT029162.1:CDS
GACTACTTACAAACTAGAAG+AGG 0.544073 5:-73809131 None:intergenic
TGTGAAGCATTGAAGCAAAA+CGG 0.548283 5:+73811175 MsaT029162.1:CDS
ACCTCCTCTTAGTGACAGCT+TGG 0.548550 5:-73810398 None:intergenic
CATACCAGTGGAAGGCGGTA+GGG 0.550508 5:+73811069 MsaT029162.1:CDS
AATTTCAGAAGAGAAGATTG+AGG 0.553548 5:+73811436 MsaT029162.1:CDS
CATACGAGATAACACTTAGG+AGG 0.553719 5:+73809312 MsaT029162.1:CDS
GAACCGGATGGGATAGGAGT+TGG 0.555156 5:-73811377 None:intergenic
GAGAAGATTGAGGAATACCC+TGG 0.559641 5:+73811446 MsaT029162.1:CDS
CTACCCCTACCGCCTTCCAC+TGG 0.563419 5:-73811073 None:intergenic
ATATCAGAAAAGGACCATCC+AGG 0.563775 5:-73811464 None:intergenic
GCATACCAGTGGAAGGCGGT+AGG 0.565793 5:+73811068 MsaT029162.1:CDS
ATACCAGTGGAAGGCGGTAG+GGG 0.568538 5:+73811070 MsaT029162.1:CDS
ACACCAACTCCTATCCCATC+CGG 0.568917 5:+73811374 MsaT029162.1:CDS
ACGACCAAGCTGTCACTAAG+AGG 0.580960 5:+73810394 MsaT029162.1:CDS
TATCAGAAAAGGACCATCCA+GGG 0.585690 5:-73811463 None:intergenic
ATACGAGATAACACTTAGGA+GGG 0.600468 5:+73809313 MsaT029162.1:CDS
AGATATAGGGAAGATTGCAT+TGG 0.616410 5:+73809103 MsaT029162.1:CDS
TAATGTAAGCCCAGAAATCG+AGG 0.616586 5:+73811213 MsaT029162.1:CDS
AGCAAAGTTCCTGATTTAGG+CGG 0.623500 5:+73810354 MsaT029162.1:CDS
AGAACATGCATACCAGTGGA+AGG 0.636991 5:+73811061 MsaT029162.1:CDS
GGATGGGATAGGAGTTGGTG+TGG 0.637023 5:-73811372 None:intergenic
GAACATACGAGATAACACTT+AGG 0.637954 5:+73809309 MsaT029162.1:CDS
AGATTGAGGAATACCCTGGA+TGG 0.653139 5:+73811450 MsaT029162.1:CDS
AGCGAGAACATGCATACCAG+TGG 0.655844 5:+73811057 MsaT029162.1:intron
TTCACCAGTCAAAGAACCTG+AGG 0.656260 5:+73811249 MsaT029162.1:CDS
CTTACAAACTAGAAGAGGCA+CGG 0.671946 5:-73809126 None:intergenic
TACGAGATAACACTTAGGAG+GGG 0.675136 5:+73809314 MsaT029162.1:CDS
ACCAAGCTGTCACTAAGAGG+AGG 0.677608 5:+73810397 MsaT029162.1:CDS
TGACGATGATGACAGCGATG+AGG 0.685240 5:+73810515 MsaT029162.1:CDS
ACATGCATACCAGTGGAAGG+CGG 0.727596 5:+73811064 MsaT029162.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 73807704 73811574 73807704 ID=MsaG029162
Chr5 mRNA 73807704 73811574 73807704 ID=MsaT029162.1;Parent=MsaG029162
Chr5 exon 73807704 73807736 73807704 ID=MsaT029162.1.exon1;Parent=MsaT029162.1
Chr5 CDS 73807704 73807736 73807704 ID=cds.MsaT029162.1;Parent=MsaT029162.1
Chr5 exon 73809071 73809143 73809071 ID=MsaT029162.1.exon2;Parent=MsaT029162.1
Chr5 CDS 73809071 73809143 73809071 ID=cds.MsaT029162.1;Parent=MsaT029162.1
Chr5 exon 73809270 73809363 73809270 ID=MsaT029162.1.exon3;Parent=MsaT029162.1
Chr5 CDS 73809270 73809363 73809270 ID=cds.MsaT029162.1;Parent=MsaT029162.1
Chr5 exon 73810302 73810418 73810302 ID=MsaT029162.1.exon4;Parent=MsaT029162.1
Chr5 CDS 73810302 73810418 73810302 ID=cds.MsaT029162.1;Parent=MsaT029162.1
Chr5 exon 73810500 73810549 73810500 ID=MsaT029162.1.exon5;Parent=MsaT029162.1
Chr5 CDS 73810500 73810549 73810500 ID=cds.MsaT029162.1;Parent=MsaT029162.1
Chr5 exon 73811059 73811273 73811059 ID=MsaT029162.1.exon6;Parent=MsaT029162.1
Chr5 CDS 73811059 73811273 73811059 ID=cds.MsaT029162.1;Parent=MsaT029162.1
Chr5 exon 73811317 73811574 73811317 ID=MsaT029162.1.exon7;Parent=MsaT029162.1
Chr5 CDS 73811317 73811574 73811317 ID=cds.MsaT029162.1;Parent=MsaT029162.1
Gene Sequence

>MsaT029162.1

ATGAGGAAGAAACTAGATACTCGTTTCCCTGCTGCTAGGATTAAGAAAATTATGCAAGCTGATGAAGATATAGGGAAGATTGCATTGGCCGTGCCTCTTCTAGTTTCAAAAGCACTAGAGCTATTTCTGCAAGATTTGTGTGATAGAACATACGAGATAACACTTAGGAGGGGAGCAAAGACCGTGAATGCTTTTCATTTAAAACAGTGCATACAAACTTGTAATGTCTTTGATTTTCTGAAAGATACTGTGAGCAAAGTTCCTGATTTAGGCGGTTCTGCTGCAACTGGTGACGACCAAGCTGTCACTAAGAGGAGGAAAGTTTTTGAAGATGACGATGATGACAGCGATGAGGAGACCAAGAGAACGAGAACATGCATACCAGTGGAAGGCGGTAGGGGTAGAGGCAGGGGACGTGGCCGCGGCCAAGGAAGTAAAACTGTAGATCAAGAGATCAATTCACATGTCAAGTTTGAAGAAGATTGTGAAGCATTGAAGCAAAACGGCAACCACACTCAAAATAATGTAAGCCCAGAAATCGAGGAAGTTAAGCTGAGTTCACCAGTCAAAGAACCTGAGGAATTCAAATTTCGATTTAAATCTGAATCGGATCCGGATGACGACATGGATGAAATATCCACACCAACTCCTATCCCATCCGGTTCATCTTCAAAATCAGTTTCAGAAGAGAAGATAAAATCAATTTCAGAAGAGAAGATTGAGGAATACCCTGGATGGTCCTTTTCTGATATGGAAAAAATGGCTATTGATCCCTTACAACTAGCCAACTTAAGTAAAAGGATTGATGAAGATATGGAAGATTATGATGAAGAAGTGTAG

Protein sequence

>MsaT029162.1

MRKKLDTRFPAARIKKIMQADEDIGKIALAVPLLVSKALELFLQDLCDRTYEITLRRGAKTVNAFHLKQCIQTCNVFDFLKDTVSKVPDLGGSAATGDDQAVTKRRKVFEDDDDDSDEETKRTRTCIPVEGGRGRGRGRGRGQGSKTVDQEINSHVKFEEDCEALKQNGNHTQNNVSPEIEEVKLSSPVKEPEEFKFRFKSESDPDDDMDEISTPTPIPSGSSSKSVSEEKIKSISEEKIEEYPGWSFSDMEKMAIDPLQLANLSKRIDEDMEDYDEEV*