AlfalfaGEDB | MsaG029181

M. sativa ssp. caerulea / MsaG029181

KEGG: K09419
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0008356 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051301 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00447.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG029181	XP_003617219.1	98.425	254	4	0	1	254	1	254	0.0	523

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG029181	sp\|Q9C635\|HSFB4_ARATH	61.364	220	53	5	20	211	29	244	1.44e-86	264

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG029181	tr\|G7KCV7\|G7KCV7_MEDTR	98.425	254	4	0	1	254	1	254	0.0	523

TFs/TRs:

Gene ID	Type	Classification
MsaG029181	TF	HSF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG029181	MtrunA17_Chr5g0441531	98.425	254	4	0	1	254	1	254	0.0	523
MsaG029181	MtrunA17_Chr3g0134031	47.941	340	83	11	1	250	1	336	5.25e-93	278
MsaG029181	MtrunA17_Chr5g0397881	44.934	227	97	4	15	215	14	238	1.32e-57	187
MsaG029181	MtrunA17_Chr1g0203601	41.129	248	121	5	17	246	10	250	5.78e-56	181
MsaG029181	MtrunA17_Chr7g0257331	45.274	201	100	2	18	218	15	205	4.01e-54	174
MsaG029181	MtrunA17_Chr4g0004151	96.386	83	3	0	172	254	1	83	2.08e-53	167
MsaG029181	MtrunA17_Chr6g0472421	39.338	272	118	6	16	249	24	286	3.70e-52	172
MsaG029181	MtrunA17_Chr5g0402291	38.367	245	121	6	18	242	2	236	3.70e-48	160
MsaG029181	MtrunA17_Chr8g0375041	38.000	250	121	6	18	254	2	230	2.87e-46	155
MsaG029181	MtrunA17_Chr5g0410881	46.597	191	90	2	22	212	29	207	4.09e-45	158
MsaG029181	MtrunA17_Chr6g0467041	69.892	93	28	0	24	116	21	113	3.10e-43	149
MsaG029181	MtrunA17_Chr1g0204741	71.134	97	28	0	23	119	38	134	3.13e-41	144
MsaG029181	MtrunA17_Chr7g0260331	65.957	94	32	0	23	116	47	140	2.59e-40	142
MsaG029181	MtrunA17_Chr1g0185131	45.029	171	63	3	23	192	30	170	2.95e-40	142
MsaG029181	MtrunA17_Chr8g0379701	66.667	99	32	1	18	116	6	103	1.94e-39	140
MsaG029181	MtrunA17_Chr8g0378061	68.132	91	29	0	26	116	23	113	1.30e-38	140
MsaG029181	MtrunA17_Chr4g0071051	43.713	167	85	3	23	180	99	265	1.31e-36	134
MsaG029181	MtrunA17_Chr4g0047421	40.361	166	87	3	22	183	30	187	3.57e-36	127
MsaG029181	MtrunA17_Chr3g0135831	42.138	159	73	1	23	181	44	183	3.63e-35	129
MsaG029181	MtrunA17_Chr6g0485571	59.184	98	40	0	19	116	5	102	3.79e-35	128
MsaG029181	MtrunA17_Chr2g0330821	58.824	102	42	0	15	116	69	170	5.39e-35	128
MsaG029181	MtrunA17_Chr4g0039891	43.949	157	75	2	25	181	12	155	5.73e-35	130
MsaG029181	MtrunA17_Chr2g0284481	57.000	100	42	1	18	117	9	107	1.03e-33	125
MsaG029181	MtrunA17_Chr8g0392001	60.417	96	37	1	24	118	21	116	2.95e-33	124
MsaG029181	MtrunA17_Chr1g0179321	65.152	66	23	0	19	84	43	108	2.02e-22	89.0
MsaG029181	MtrunA17_Chr4g0023771	62.069	58	22	0	25	82	15	72	3.74e-19	80.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG029181	AT1G46264.1	61.364	220	53	5	20	211	29	244	1.46e-87	264
MsaG029181	AT4G36990.1	39.286	252	133	4	12	247	1	248	9.00e-54	175
MsaG029181	AT5G62020.1	40.000	250	131	4	18	249	16	264	2.49e-52	172
MsaG029181	AT4G11660.1	71.845	103	29	0	17	119	51	153	2.14e-50	169
MsaG029181	AT1G32330.2	46.196	184	87	3	22	204	34	206	5.32e-45	157
MsaG029181	AT1G32330.1	46.196	184	87	3	22	204	34	206	6.20e-45	157
MsaG029181	AT2G41690.1	43.094	181	93	3	23	194	38	217	9.19e-44	148
MsaG029181	AT4G17750.1	64.486	107	38	0	15	121	42	148	1.51e-43	154
MsaG029181	AT3G22830.1	66.327	98	33	0	20	117	56	153	4.10e-40	143
MsaG029181	AT3G02990.1	66.667	96	31	1	21	116	20	114	3.45e-39	142
MsaG029181	AT5G16820.1	67.000	100	31	2	21	120	24	121	3.49e-39	142
MsaG029181	AT5G16820.2	67.000	100	31	2	21	120	24	121	3.49e-39	142
MsaG029181	AT5G43840.1	60.952	105	41	0	23	127	17	121	4.77e-39	137
MsaG029181	AT5G03720.2	63.265	98	36	0	25	122	55	152	2.39e-38	138
MsaG029181	AT5G03720.1	63.265	98	36	0	25	122	55	152	2.39e-38	138
MsaG029181	AT3G63350.1	36.111	216	121	4	19	221	22	233	4.19e-38	135
MsaG029181	AT2G26150.1	59.794	97	39	0	20	116	39	135	4.89e-37	134
MsaG029181	AT2G26150.4	59.794	97	39	0	20	116	39	135	4.89e-37	134
MsaG029181	AT3G51910.1	60.417	96	38	0	20	115	24	119	5.15e-36	129
MsaG029181	AT4G13980.1	64.835	91	32	0	26	116	24	114	1.11e-35	132
MsaG029181	AT5G54070.1	59.783	92	37	0	24	115	70	161	2.87e-35	129
MsaG029181	AT3G24520.1	60.000	95	38	0	22	116	14	108	5.99e-35	128
MsaG029181	AT1G67970.1	48.800	125	63	1	24	147	18	142	6.19e-34	126
MsaG029181	AT4G18880.1	65.657	99	33	1	18	116	9	106	1.25e-31	120
MsaG029181	AT5G45710.3	42.442	172	91	3	25	191	13	181	3.55e-31	118
MsaG029181	AT5G45710.1	42.442	172	91	3	25	191	13	181	3.55e-31	118
MsaG029181	AT4G18870.1	44.706	85	47	0	25	109	146	230	4.14e-20	87.4
MsaG029181	AT4G18870.1	38.596	114	62	3	26	137	14	121	2.89e-18	82.4

Find 37 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATATCAGTGTTTGATTT+AGG	0.203557	5:+74163252	None:intergenic
CTCTTTGGTGTTCCTCTTTG+TGG	0.237760	5:-74162988	MsaT029181.1:CDS
AGCTCTTCTGTGAAGCTCTT+TGG	0.317364	5:-74163003	MsaT029181.1:CDS
CTATTACAATCTTTATCATT+TGG	0.339266	5:+74163024	None:intergenic
ATGAAACTACATTTGTTGTT+TGG	0.362655	5:-74164055	MsaT029181.1:CDS
AGAAGATCTCTAGCAAATTC+AGG	0.373696	5:+74164026	None:intergenic
TCTTTATCATTTGGTAATTG+AGG	0.392241	5:+74163033	None:intergenic
CTTTGTTCAAAGGGTGAAGC+AGG	0.399996	5:+74163108	None:intergenic
ATTGTTGCTTCTTTGTTCAA+AGG	0.403141	5:+74163098	None:intergenic
TCTTATTAATCAAGATTACT+AGG	0.404553	5:+74162949	None:intergenic
ACAACTTCTCAAGCTTTGTT+AGG	0.420026	5:-74163983	MsaT029181.1:CDS
ATCCAGCATATACTTTCATC+TGG	0.441694	5:+74163294	None:intergenic
TTGTTGCTTCTTTGTTCAAA+GGG	0.445911	5:+74163099	None:intergenic
TCAAGAAGGTTGTTGCTGAT+AGG	0.447682	5:-74163440	MsaT029181.1:CDS
AAACTACATTTGTTGTTTGG+AGG	0.447885	5:-74164052	MsaT029181.1:CDS
TGGTATGTCTTTGTCAAGAA+AGG	0.453363	5:+74164125	None:intergenic
CTTCTTGCAGGGTTTCAAGA+AGG	0.454510	5:-74163454	MsaT029181.1:intron
ATTGTTGTTGATAGTGTTGA+GGG	0.457477	5:+74163346	None:intergenic
AGATAACATGGTGTTCACTA+TGG	0.459097	5:-74164171	MsaT029181.1:CDS
TCACTGACCACATTGTATCT+TGG	0.491347	5:-74164085	MsaT029181.1:CDS
GTCTTTGTCAAGAAAGGAGC+AGG	0.498324	5:+74164131	None:intergenic
TTTGATTTAGGAGATAGTAA+TGG	0.501932	5:+74163264	None:intergenic
CTGAGGATAATCAAAGACTA+AGG	0.512230	5:-74163218	MsaT029181.1:CDS
AACCAGATGAAAGTATATGC+TGG	0.518742	5:-74163296	MsaT029181.1:CDS
ATCTATTATGTGAAATCCAC+AGG	0.520532	5:-74163377	MsaT029181.1:CDS
AGAAGGTTGTTGCTGATAGG+TGG	0.520569	5:-74163437	MsaT029181.1:CDS
TATCTTAACAGCACTTTCTG+AGG	0.572199	5:-74163235	MsaT029181.1:CDS
GAAGGTTGTTGCTGATAGGT+GGG	0.581284	5:-74163436	MsaT029181.1:CDS
TATTGTTGTTGATAGTGTTG+AGG	0.584009	5:+74163345	None:intergenic
TGAGAGGATCATCAACAAGT+TGG	0.584975	5:+74164105	None:intergenic
GATACAATGTGGTCAGTGAG+AGG	0.630246	5:+74164089	None:intergenic
TGATGGCTTTCACATTAGAG+AGG	0.643157	5:-74164199	MsaT029181.1:CDS
AGAGAGGTGTGAAGATAACA+TGG	0.644284	5:-74164183	MsaT029181.1:CDS
TTGTTGTTGATAGTGTTGAG+GGG	0.651120	5:+74163347	None:intergenic
AGATCTCTAGCAAATTCAGG+AGG	0.698266	5:+74164029	None:intergenic
AGGGTGAAGCAGGACTCACA+TGG	0.700311	5:+74163118	None:intergenic
CATCACTCCAAGATACAATG+TGG	0.717706	5:+74164078	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	74162954	74164219	74162954	ID=MsaG029181
Chr5	mRNA	74162954	74164219	74162954	ID=MsaT029181.1;Parent=MsaG029181
Chr5	exon	74162954	74163466	74162954	ID=MsaT029181.1.exon2;Parent=MsaT029181.1
Chr5	CDS	74162954	74163466	74162954	ID=cds.MsaT029181.1;Parent=MsaT029181.1
Chr5	exon	74163968	74164219	74163968	ID=MsaT029181.1.exon1;Parent=MsaT029181.1
Chr5	CDS	74163968	74164219	74163968	ID=cds.MsaT029181.1;Parent=MsaT029181.1

Gene Sequence

>MsaT029181.1

ATGGCTTTCACATTAGAGAGGTGTGAAGATAACATGGTGTTCACTATGGAGTCTCAGAAATCAGTGCCTGCTCCTTTCTTGACAAAGACATACCAACTTGTTGATGATCCTCTCACTGACCACATTGTATCTTGGAGTGATGATGAAACTACATTTGTTGTTTGGAGGCCTCCTGAATTTGCTAGAGATCTTCTTCCCAATTTTTTCAAGCACAACAACTTCTCAAGCTTTGTTAGGCAACTAAATACATATGGTTTCAAGAAGGTTGTTGCTGATAGGTGGGAGTTTGCAAATGATTACTTCAAAAAAGGAGCTAAGCATCTATTATGTGAAATCCACAGGAGAAAAACCCCTCAACACTATCAACAACAATACTATGAACAATCACCACAAATTTTTCAACCAGATGAAAGTATATGCTGGATTGATTCTCCATTACTATCTCCTAAATCAAACACTGATATCTTAACAGCACTTTCTGAGGATAATCAAAGACTAAGGAGAAAGAATTTCATGCTGTTATCAGAACTCAGTCACATGAAGAATCTTTACAATGATATTATATATTTCATTCAAAACCATGTGAGTCCTGCTTCACCCTTTGAACAAAGAAGCAACAATAGTGCTGCTATTTTGAAGCTTATTGAATTGGATTCTGCATCACCTCAATTACCAAATGATAAAGATTGTAATAGCTCTTCTGTGAAGCTCTTTGGTGTTCCTCTTTGTGGAAAAAAGAGGGTTCATCCTAGTAATCTTGATTAA

Protein sequence

>MsaT029181.1

MAFTLERCEDNMVFTMESQKSVPAPFLTKTYQLVDDPLTDHIVSWSDDETTFVVWRPPEFARDLLPNFFKHNNFSSFVRQLNTYGFKKVVADRWEFANDYFKKGAKHLLCEIHRRKTPQHYQQQYYEQSPQIFQPDESICWIDSPLLSPKSNTDILTALSEDNQRLRRKNFMLLSELSHMKNLYNDIIYFIQNHVSPASPFEQRSNNSAAILKLIELDSASPQLPNDKDCNSSSVKLFGVPLCGKKRVHPSNLD*