AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029184


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029184 MtrunA17_Chr5g0441581 90.272 257 25 0 136 392 424 680 1.31e-165 484
MsaG029184 MtrunA17_Chr5g0441581 97.351 151 4 0 1 151 1 151 6.95e-101 317
MsaG029184 MtrunA17_Chr6g0451441 63.636 154 45 3 7 151 61 212 2.64e-57 197
MsaG029184 MtrunA17_Chr1g0172041 68.142 113 36 0 208 320 1 113 9.85e-51 168
MsaG029184 MtrunA17_Chr6g0477261 39.604 202 121 1 136 336 285 486 1.23e-37 146
MsaG029184 MtrunA17_Chr2g0296781 38.614 202 123 1 136 336 396 597 9.89e-36 141
MsaG029184 MtrunA17_Chr1g0196541 36.150 213 123 3 140 351 406 606 7.74e-30 123
MsaG029184 MtrunA17_Chr5g0441591 88.889 63 7 0 421 483 1 63 8.38e-29 109
MsaG029184 MtrunA17_Chr5g0441571 71.429 77 14 1 420 488 227 303 3.65e-23 102
MsaG029184 MtrunA17_Chr5g0441651 68.831 77 16 1 420 488 235 311 2.36e-22 100
MsaG029184 MtrunA17_Chr1g0207551 31.414 191 114 4 136 322 436 613 1.45e-18 89.0
MsaG029184 MtrunA17_Chr3g0080351 29.756 205 129 3 136 337 470 662 6.57e-18 87.0
MsaG029184 MtrunA17_Chr5g0448761 29.237 236 148 6 136 360 471 698 1.12e-17 86.3
MsaG029184 MtrunA17_Chr6g0476441 39.604 101 60 1 237 336 2 102 4.49e-16 79.0
MsaG029184 MtrunA17_Chr3g0118611 29.464 224 141 6 152 371 363 573 5.71e-16 80.9
MsaG029184 MtrunA17_Chr2g0291581 26.860 242 164 6 117 349 443 680 1.21e-15 79.7
MsaG029184 MtrunA17_Chr5g0448511 31.288 163 101 4 136 295 243 397 2.24e-15 78.6
MsaG029184 MtrunA17_Chr5g0440451 29.843 191 122 4 152 337 466 649 6.69e-14 74.7
MsaG029184 MtrunA17_Chr2g0329031 27.848 237 150 7 115 347 395 614 7.08e-14 74.3
MsaG029184 MtrunA17_Chr2g0287061 33.846 130 74 4 168 293 578 699 7.53e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029184 AT5G12200.1 63.636 77 20 1 420 488 223 299 1.98e-20 94.7
MsaG029184 AT3G22170.2 26.531 245 148 4 113 337 424 656 4.86e-18 87.8
MsaG029184 AT3G22170.1 26.531 245 148 4 113 337 424 656 4.86e-18 87.8
MsaG029184 AT4G15090.1 27.642 246 157 5 136 376 456 685 8.27e-18 87.0
MsaG029184 AT4G15090.5 27.642 246 157 5 136 376 456 685 8.27e-18 87.0
MsaG029184 AT4G15090.3 27.642 246 157 5 136 376 456 685 8.27e-18 87.0
MsaG029184 AT4G15090.2 27.642 246 157 5 136 376 456 685 8.27e-18 87.0
MsaG029184 AT4G15090.4 27.642 246 157 5 136 376 456 685 8.27e-18 87.0
MsaG029184 AT2G32250.2 31.472 197 122 5 153 347 417 602 3.69e-16 82.0
MsaG029184 AT2G32250.4 31.472 197 122 5 153 347 417 602 3.69e-16 82.0
MsaG029184 AT2G32250.5 31.472 197 122 5 153 347 417 602 3.79e-16 82.0
MsaG029184 AT2G32250.6 31.472 197 122 5 153 347 417 602 4.03e-16 82.0
MsaG029184 AT2G32250.1 31.472 197 122 5 153 347 417 602 4.03e-16 82.0
MsaG029184 AT2G32250.3 31.472 197 122 5 153 347 417 602 4.03e-16 82.0
MsaG029184 AT2G32250.8 31.472 197 122 5 153 347 376 561 4.11e-16 81.6
MsaG029184 AT2G32250.7 31.472 197 122 5 153 347 376 561 4.45e-16 81.6
MsaG029184 AT4G38170.1 33.333 144 91 2 152 295 261 399 5.54e-16 80.9
MsaG029184 AT4G38180.1 32.867 143 91 2 153 295 497 634 1.76e-15 79.7
MsaG029184 AT1G52520.1 27.586 232 152 4 119 347 472 690 4.29e-15 78.6
MsaG029184 AT1G76320.3 24.265 272 194 4 153 423 410 670 9.41e-12 67.8
MsaG029184 AT1G76320.2 27.638 199 129 5 153 347 410 597 1.97e-11 67.0
MsaG029184 AT1G76320.1 27.638 199 129 5 153 347 410 597 2.06e-11 67.0
MsaG029184 AT1G76320.4 27.638 199 129 5 153 347 410 597 2.06e-11 67.0
MsaG029184 AT2G27110.2 26.203 187 128 4 153 337 442 620 8.21e-11 65.1
MsaG029184 AT2G27110.1 26.203 187 128 4 153 337 442 620 8.21e-11 65.1
MsaG029184 AT2G27110.3 28.966 145 94 3 153 295 297 434 9.12e-11 64.7

Find 80 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTGAACTTCTTTGAATT+TGG 0.140264 5:-74192896 None:intergenic
TAGAGAGTATGCTTTGAAAA+AGG 0.184139 5:+74191592 MsaT029184.1:CDS
CGAGGGAGCACAATTCATTT+TGG 0.189374 5:-74192929 None:intergenic
CTTGGACTAACTGGTCTTAA+AGG 0.272670 5:+74194562 MsaT029184.1:CDS
TAAATTGATGTAATGTTTCA+TGG 0.277147 5:-74193335 None:intergenic
CTTCAAAGTTGTGTTTAATC+AGG 0.287821 5:+74193032 MsaT029184.1:CDS
GACCAGCCCTACAAAGAAAA+TGG 0.313866 5:+74191835 MsaT029184.1:CDS
TGTTCGTTGTTTGAGTTTAG+AGG 0.315013 5:+74193078 MsaT029184.1:CDS
ATGATAGAACTTGGACTAAC+TGG 0.323580 5:+74194553 MsaT029184.1:CDS
TGAATTCCAGCACCATTGTT+TGG 0.354475 5:-74193417 None:intergenic
TTCCAACTTAATACAAATCT+TGG 0.361351 5:-74191762 None:intergenic
TGCCAAGATTTGTATTAAGT+TGG 0.381892 5:+74191760 MsaT029184.1:CDS
TTGAAACTTCATGGATAGTT+CGG 0.390728 5:+74191439 None:intergenic
TGGAATTCACAGTCCATTGC+GGG 0.395864 5:+74193431 MsaT029184.1:CDS
TTAAAGGACATCTTCTTTCA+AGG 0.412333 5:+74194578 MsaT029184.1:CDS
GCATGTTGGAAGCCTGCAAC+GGG 0.415038 5:+74191530 MsaT029184.1:CDS
CTTTGCTACTTATCATGTGT+TGG 0.420954 5:+74192972 MsaT029184.1:CDS
TGCTACTTATCATGTGTTGG+AGG 0.424099 5:+74192975 MsaT029184.1:CDS
GCCATGGTCCATGCAGGAAA+TGG 0.426039 5:+74194502 MsaT029184.1:intron
ATCGTGAAGGTTCTAAAATC+AGG 0.426549 5:+74194810 MsaT029184.1:CDS
AATGTGTAGGAGTGCAATAT+TGG 0.431270 5:+74193589 MsaT029184.1:intron
CTGTCTGTCATTACTTCGCT+TGG 0.445713 5:-74193480 None:intergenic
AACATTCACAGCTGAAATCA+TGG 0.450961 5:-74193059 None:intergenic
TTGGAGGAGGTTGTAGTTGG+AGG 0.455878 5:+74192991 MsaT029184.1:CDS
ACATCAATTTAAGTGTAGTA+AGG 0.462673 5:+74193347 MsaT029184.1:CDS
CAACAGAAAATGATAGAACT+TGG 0.466596 5:+74194544 MsaT029184.1:CDS
ATTGAGGTTGTCAGTGGTGG+TGG 0.468328 5:+74193658 MsaT029184.1:CDS
TGTATTAAGTTGGAAAAGGA+TGG 0.471002 5:+74191770 MsaT029184.1:CDS
AGTTGGAAAAGGATGGTTTG+TGG 0.490374 5:+74191777 MsaT029184.1:CDS
GTGTTGGAGGAGGTTGTAGT+TGG 0.490830 5:+74192988 MsaT029184.1:CDS
GATTTGTATTAAGTTGGAAA+AGG 0.491914 5:+74191766 MsaT029184.1:CDS
TCAAATCCAAACAATGGTGC+TGG 0.496584 5:+74193411 MsaT029184.1:CDS
AATTGAAGCCACAGATGGAC+AGG 0.502360 5:+74193238 MsaT029184.1:CDS
AACTTAATACAAATCTTGGC+AGG 0.506782 5:-74191758 None:intergenic
ATCTGTGTGTTCACGAGAGA+GGG 0.507673 5:+74193122 MsaT029184.1:CDS
TGTAATGAGCATTGATGTAA+TGG 0.508685 5:+74194782 MsaT029184.1:CDS
ATTCTTGTTTGCACAAGAGA+GGG 0.511572 5:+74191674 MsaT029184.1:CDS
AATGGAGATGATGTTTATGC+AGG 0.512599 5:+74194520 MsaT029184.1:CDS
AGAATCTGAAGATGCAACTA+AGG 0.521210 5:+74193308 MsaT029184.1:CDS
CGGGTTAAATGTGAAGATCA+AGG 0.521428 5:+74193450 MsaT029184.1:CDS
TACTTATCATGTGTTGGAGG+AGG 0.523219 5:+74192978 MsaT029184.1:CDS
GCCTTAGCCATGGTCCATGC+AGG 0.523996 5:+74194496 MsaT029184.1:intron
ATCGAAATCAATGATGATGC+AGG 0.524055 5:+74191878 MsaT029184.1:CDS
GCAAACAAGAATTGCATAGC+AGG 0.525492 5:-74191663 None:intergenic
TGCACAAGAGAGGGGTCTCA+AGG 0.531486 5:+74191683 MsaT029184.1:CDS
ATCTATGTAGTTGGAAGGAG+AGG 0.532620 5:+74194725 MsaT029184.1:intron
TCTTCACATTTAACCCGCAA+TGG 0.533346 5:-74193444 None:intergenic
TAGTATTCATGATGAAGGTA+TGG 0.538379 5:+74191481 MsaT029184.1:CDS
CCCTCGTTGAATGTTGTCGA+GGG 0.543640 5:+74192946 MsaT029184.1:CDS
TGTGATAGTATTCATGATGA+AGG 0.546656 5:+74191476 MsaT029184.1:CDS
CAATTTAAGTGTAGTAAGGA+TGG 0.555667 5:+74193351 MsaT029184.1:CDS
TTTATATCTATGTAGTTGGA+AGG 0.557118 5:+74194720 MsaT029184.1:intron
TATCTGTGTGTTCACGAGAG+AGG 0.565938 5:+74193121 MsaT029184.1:CDS
TTGTCAAACCTGTCCATCTG+TGG 0.569297 5:-74193246 None:intergenic
CATCATCTCCATTTCCTGCA+TGG 0.572541 5:-74194510 None:intergenic
TCCCTCGTTGAATGTTGTCG+AGG 0.574922 5:+74192945 MsaT029184.1:CDS
CTGGAATTCACAGTCCATTG+CGG 0.575404 5:+74193430 MsaT029184.1:CDS
TGGCATGAGTGTACTCACCT+TGG 0.576261 5:-74192876 None:intergenic
AATTCTTGTTTGCACAAGAG+AGG 0.579543 5:+74191673 MsaT029184.1:CDS
CTATGTAGAATCATCTATTG+AGG 0.583723 5:+74193642 MsaT029184.1:CDS
TCCATTTCCTGCATGGACCA+TGG 0.584019 5:-74194503 None:intergenic
AGCATGTTGGAAGCCTGCAA+CGG 0.586912 5:+74191529 MsaT029184.1:CDS
TCCATTGAAAAGCAATTCCA+AGG 0.588119 5:+74192859 MsaT029184.1:CDS
AGTTGCATCTTCAGATTCTG+CGG 0.590083 5:-74193304 None:intergenic
TCTATTGAGGTTGTCAGTGG+TGG 0.597149 5:+74193655 MsaT029184.1:CDS
ATGGAGATGATGTTTATGCA+GGG 0.598515 5:+74194521 MsaT029184.1:CDS
CTGATGATGATGAAGCATGT+TGG 0.601790 5:+74191516 MsaT029184.1:CDS
TCTTCTTTCAAGGCGTGCAG+TGG 0.604982 5:+74194588 MsaT029184.1:CDS
GATGACTCAAATCCAAACAA+TGG 0.622244 5:+74193405 MsaT029184.1:CDS
TTCTTGTTTGCACAAGAGAG+GGG 0.624091 5:+74191675 MsaT029184.1:CDS
GCACAAGAGAGGGGTCTCAA+GGG 0.625092 5:+74191684 MsaT029184.1:CDS
GAGAGAATTGAAGCCACAGA+TGG 0.631735 5:+74193233 MsaT029184.1:CDS
TCATCTATTGAGGTTGTCAG+TGG 0.640747 5:+74193652 MsaT029184.1:CDS
CCCTCGACAACATTCAACGA+GGG 0.645738 5:-74192946 None:intergenic
CAGATAACACATGACAACAC+AGG 0.666939 5:-74193104 None:intergenic
TCGCGCTATTCACTTATACG+TGG 0.671636 5:+74194755 MsaT029184.1:CDS
GTAGGAGTGCAATATTGGAA+TGG 0.673951 5:+74193594 MsaT029184.1:intron
TGTAATGGAAGAAATCGTGA+AGG 0.687566 5:+74194797 MsaT029184.1:CDS
CATGTTGGAAGCCTGCAACG+GGG 0.757747 5:+74191531 MsaT029184.1:CDS
ACCCTCGACAACATTCAACG+AGG 0.768012 5:-74192947 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 74191449 74194845 74191449 ID=MsaG029184
Chr5 mRNA 74191449 74194845 74191449 ID=MsaT029184.1;Parent=MsaG029184
Chr5 exon 74191449 74191901 74191449 ID=MsaT029184.1.exon1;Parent=MsaT029184.1
Chr5 CDS 74191449 74191901 74191449 ID=cds.MsaT029184.1;Parent=MsaT029184.1
Chr5 exon 74192766 74193488 74192766 ID=MsaT029184.1.exon2;Parent=MsaT029184.1
Chr5 CDS 74192766 74193488 74192766 ID=cds.MsaT029184.1;Parent=MsaT029184.1
Chr5 exon 74193598 74193679 74193598 ID=MsaT029184.1.exon3;Parent=MsaT029184.1
Chr5 CDS 74193598 74193679 74193598 ID=cds.MsaT029184.1;Parent=MsaT029184.1
Chr5 exon 74194503 74194609 74194503 ID=MsaT029184.1.exon4;Parent=MsaT029184.1
Chr5 CDS 74194503 74194609 74194503 ID=cds.MsaT029184.1;Parent=MsaT029184.1
Chr5 exon 74194735 74194845 74194735 ID=MsaT029184.1.exon5;Parent=MsaT029184.1
Chr5 CDS 74194735 74194845 74194735 ID=cds.MsaT029184.1;Parent=MsaT029184.1
Gene Sequence

>MsaT029184.1

ATGGATAGTTCGGAGAATTTAGTAGACTGTGATAGTATTCATGATGAAGGTATGGAAGTTGATAACTCTGATGATGATGAAGCATGTTGGAAGCCTGCAACGGGGATGCGTTTTTCTTGCATAGATGATGTCAAGACACTTTATAGAGAGTATGCTTTGAAAAAGGGTTTTCGGTGGAAGACCAGAACATCGAAAAAAAGAGATGATGGAGAGACCTGCTATGCAATTCTTGTTTGCACAAGAGAGGGGTCTCAAGGGTCAGAAATTCCGTGTACGTTAAAGACACCCCCACCTAAGACAAAAAATTGTCCTGCCAAGATTTGTATTAAGTTGGAAAAGGATGGTTTGTGGTACATTTCAAAATTTGAATCTAGACATTCTCACAAGACCAGCCCTACAAAGAAAATGGACTTGCATGTAAAGAGAACAATCGAAATCAATGATGATGCAGGACAATATGATAATGCTCTTAGTAGTCGAGCAGAAAAAGAATTTGAAGCGGATTTTAATTCACTGGATACTACAATTCAGTGTGTGTCAAACTCGTCCATTGAAAAGCAATTCCAAGGTGAGTACACTCATGCCAAATTCAAAGAAGTTCAAGCTGAATTCATATCCAAAATGAATTGTGCTCCCTCGTTGAATGTTGTCGAGGGTTGCTTTGCTACTTATCATGTGTTGGAGGAGGTTGTAGTTGGAGGCAGACATAAAGAAAATGTCTTCAAAGTTGTGTTTAATCAGGAAAACCATGATTTCAGCTGTGAATGTTCGTTGTTTGAGTTTAGAGGTATCCTGTGTTGTCATGTGTTATCTGTGTGTTCACGAGAGAGGGTTAAAAATGTGGCAGAGAAGTATGTTTTAACAAGATGGAAGAAAAATATTAAAAGGAAGCATTCATATATTAAGAGTAGTTATTGCGCGAGAGAATTGAAGCCACAGATGGACAGGTTTGACAAATTGTGCAAGCATTTCTATGAGATTGCTGAAGTAGCCGCAGAATCTGAAGATGCAACTAAGGCACTCCATGAAACATTACATCAATTTAAGTGTAGTAAGGATGGTATAACTGACATTGTTAACAGAAGTTTCATTGATGACTCAAATCCAAACAATGGTGCTGGAATTCACAGTCCATTGCGGGTTAAATGTGAAGATCAAGGAAATGAACCAAGCGAAGAGTGCAATATTGGAATGGTGAGAAATCAGATCGAAAGCAACATCTATGTAGAATCATCTATTGAGGTTGTCAGTGGTGGTGCCATGGTCCATGCAGGAAATGGAGATGATGTTTATGCAGGGCAACAGAAAATGATAGAACTTGGACTAACTGGTCTTAAAGGACATCTTCTTTCAAGGCGTGCAGTGTTGGAAGGAGAGGCAACTTCTCGCGCTATTCACTTATACGTGGTTCATGTAATGAGCATTGATGTAATGGAAGAAATCGTGAAGGTTCTAAAATCAGGTTCTTCGTCTTAA

Protein sequence

>MsaT029184.1

MDSSENLVDCDSIHDEGMEVDNSDDDEACWKPATGMRFSCIDDVKTLYREYALKKGFRWKTRTSKKRDDGETCYAILVCTREGSQGSEIPCTLKTPPPKTKNCPAKICIKLEKDGLWYISKFESRHSHKTSPTKKMDLHVKRTIEINDDAGQYDNALSSRAEKEFEADFNSLDTTIQCVSNSSIEKQFQGEYTHAKFKEVQAEFISKMNCAPSLNVVEGCFATYHVLEEVVVGGRHKENVFKVVFNQENHDFSCECSLFEFRGILCCHVLSVCSRERVKNVAEKYVLTRWKKNIKRKHSYIKSSYCARELKPQMDRFDKLCKHFYEIAEVAAESEDATKALHETLHQFKCSKDGITDIVNRSFIDDSNPNNGAGIHSPLRVKCEDQGNEPSEECNIGMVRNQIESNIYVESSIEVVSGGAMVHAGNGDDVYAGQQKMIELGLTGLKGHLLSRRAVLEGEATSRAIHLYVVHVMSIDVMEEIVKVLKSGSSS*