AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029417


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029417 MtrunA17_Chr5g0407561 83.849 291 35 4 148 429 254 541 2.44e-167 483
MsaG029417 MtrunA17_Chr5g0407561 79.618 157 17 3 1 148 1 151 3.54e-67 225
MsaG029417 MtrunA17_Chr3g0144341 45.646 333 131 11 116 429 7 308 8.11e-71 228
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029417 AT2G17410.2 54.118 255 87 7 188 429 480 717 4.56e-79 261
MsaG029417 AT2G17410.1 53.307 257 92 7 188 429 480 723 5.38e-79 261
MsaG029417 AT2G17410.3 53.307 257 92 7 188 429 480 723 5.38e-79 261
MsaG029417 AT1G76510.2 48.560 243 107 5 199 429 137 373 3.05e-61 205
MsaG029417 AT1G76510.4 48.560 243 107 5 199 429 137 373 3.05e-61 205
MsaG029417 AT1G76510.1 48.560 243 107 5 199 429 137 373 3.05e-61 205
MsaG029417 AT1G20910.1 46.914 243 111 5 199 429 101 337 5.95e-56 190
MsaG029417 AT1G20910.2 43.165 278 139 6 164 429 67 337 1.04e-55 189
MsaG029417 AT1G76510.3 44.361 266 107 6 199 429 137 396 3.64e-55 191

Find 95 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCCATTCTCCATAAAATT+TGG 0.109421 5:-76822019 None:intergenic
AATCTTGCTGGTTTGGAATT+AGG 0.186518 5:+76821469 MsaT029417.1:CDS
CCACAGTTTCCTGGACTTTC+CGG 0.219944 5:+76823758 MsaT029417.1:CDS
AGGAGCTTGAAAACTTCTTT+AGG 0.247131 5:+76821971 MsaT029417.1:CDS
AACATGTTCTGCTGCGAATC+CGG 0.285848 5:-76821877 None:intergenic
CTTCTTTAGGGAGAGGAGTA+TGG 0.293766 5:+76821984 MsaT029417.1:CDS
GAAATATATGCTTTAGTTCC+TGG 0.296044 5:+76825608 MsaT029417.1:CDS
GGACTTGCACCACAGTTTCC+TGG 0.301953 5:+76823749 MsaT029417.1:intron
ACGTAAGAGCCCATCTTATA+TGG 0.307472 5:+76825141 MsaT029417.1:CDS
TATGAAAGGCATAACATAAA+AGG 0.325509 5:+76824095 MsaT029417.1:CDS
TATTGATAATCTTGCTGGTT+TGG 0.333193 5:+76821462 MsaT029417.1:CDS
GCCTGTGAATGTTGAAAATC+AGG 0.345539 5:+76824154 MsaT029417.1:CDS
GGAGCTTGAAAACTTCTTTA+GGG 0.352045 5:+76821972 MsaT029417.1:CDS
AGCCATTCTCCATAAAATTT+GGG 0.353493 5:-76822018 None:intergenic
ATTCCTGTCCTTGTATCATC+AGG 0.357516 5:-76821784 None:intergenic
TAAGATGGGCTCTTACGTTT+AGG 0.359154 5:-76825136 None:intergenic
TTCCAAACCCAGAAAATAGA+AGG 0.366855 5:-76824709 None:intergenic
CATAGGACCTTCTGCTGATT+GGG 0.368858 5:+76825464 MsaT029417.1:CDS
CAAAGTGGGTCAGGGTGTTC+CGG 0.378330 5:+76821360 MsaT029417.1:CDS
ACATAGGACCTTCTGCTGAT+TGG 0.380929 5:+76825463 MsaT029417.1:CDS
GTGTCAGATGATGTCAAAAC+TGG 0.381353 5:+76821580 MsaT029417.1:CDS
GAAGTTTCCTCTACAGTAAC+CGG 0.388879 5:-76821541 None:intergenic
ATGATTATTGATAATCTTGC+TGG 0.390384 5:+76821457 MsaT029417.1:CDS
TGTCTTAACTGTGGTCTTGA+AGG 0.397597 5:-76825193 None:intergenic
ACCTCCTCCCGCAGAAGGCC+AGG 0.415357 5:-76825626 None:intergenic
ACTGCATTATCCATATAAGA+TGG 0.421547 5:-76825151 None:intergenic
AGAAAACGACAAAGTGGGTC+AGG 0.425358 5:+76821351 MsaT029417.1:CDS
GGCCTTCTGCGGGAGGAGGT+TGG 0.443652 5:+76825629 MsaT029417.1:CDS
TGTTCAAGATACCCTTCATT+CGG 0.446726 5:+76821675 MsaT029417.1:CDS
ATACCTTCTGAAACAACCTT+GGG 0.449374 5:-76821640 None:intergenic
GATGCTGATCATTATTATGA+TGG 0.450037 5:+76821907 MsaT029417.1:CDS
ATGGAGAGCTGTGATGAGAT+TGG 0.450926 5:+76822167 MsaT029417.1:CDS
ACATGTTCTGCTGCGAATCC+GGG 0.460181 5:-76821876 None:intergenic
TTTAGTTCCTGGCCTTCTGC+GGG 0.466389 5:+76825619 MsaT029417.1:CDS
TCCTGGCCTTCTGCGGGAGG+AGG 0.468253 5:+76825625 MsaT029417.1:CDS
GATGCATCTTGAAGTTCAAC+CGG 0.489348 5:+76821522 MsaT029417.1:CDS
CAGAAAATAGAAGGAGTTCT+CGG 0.490353 5:-76824700 None:intergenic
TACAAAAGAAAACGACAAAG+TGG 0.494694 5:+76821345 MsaT029417.1:CDS
TCACCTTCTCATAAAATCTC+CGG 0.500478 5:-76823777 None:intergenic
AATACCTTCTGAAACAACCT+TGG 0.501857 5:-76821641 None:intergenic
TTGAAAACTTCTTTAGGGAG+AGG 0.501970 5:+76821977 MsaT029417.1:CDS
TGGAGAGCTGTGATGAGATT+GGG 0.502339 5:+76822168 MsaT029417.1:CDS
TATTATGATGGAAATGAGTC+GGG 0.503594 5:+76821919 MsaT029417.1:CDS
GAAAATGAAAATTCAAAACT+AGG 0.506491 5:+76821421 MsaT029417.1:CDS
TATGCCTTTCATAATCAAGA+AGG 0.508041 5:-76824085 None:intergenic
TCCTCTACATTAACATTCGC+CGG 0.508778 5:-76821379 None:intergenic
CTTTAGTTCCTGGCCTTCTG+CGG 0.509492 5:+76825618 MsaT029417.1:CDS
CAGCCGCCTCAACCGAATGA+AGG 0.516023 5:-76821687 None:intergenic
TCATATGTTGGACACAGCTA+AGG 0.516542 5:+76821714 MsaT029417.1:CDS
TTATTATGATGGAAATGAGT+CGG 0.516599 5:+76821918 MsaT029417.1:CDS
AATCATTCAACATAATAGAA+AGG 0.520780 5:-76822221 None:intergenic
AGATACCCTTCATTCGGTTG+AGG 0.521109 5:+76821681 MsaT029417.1:CDS
AGGCCCTTCTTGATTATGAA+AGG 0.535189 5:+76824081 MsaT029417.1:intron
GTCCTTGTATCATCAGGGGC+AGG 0.543099 5:-76821778 None:intergenic
CTCCAACCTCCTCCCGCAGA+AGG 0.545798 5:-76825631 None:intergenic
AGTTCCTGGCCTTCTGCGGG+AGG 0.546177 5:+76825622 MsaT029417.1:CDS
TTAGAAGTTGAAAGTAAGGA+CGG 0.547589 5:+76821817 MsaT029417.1:CDS
AAACCCAAGGTTGTTTCAGA+AGG 0.551480 5:+76821637 MsaT029417.1:CDS
ATAAAATCTCCGGAAAGTCC+AGG 0.551594 5:-76823767 None:intergenic
CTGCATTATCCATATAAGAT+GGG 0.554084 5:-76825150 None:intergenic
AGTTCAACCGGTTACTGTAG+AGG 0.554846 5:+76821534 MsaT029417.1:CDS
GAGGAAGCTGACCATGTCAT+TGG 0.555265 5:+76821397 MsaT029417.1:CDS
TTCCTGCCCCTGATGATACA+AGG 0.557681 5:+76821776 MsaT029417.1:CDS
GAGATTGGGAGGCTATGAGA+AGG 0.562330 5:+76822182 MsaT029417.1:CDS
TTCCTGTCCTTGTATCATCA+GGG 0.567796 5:-76821783 None:intergenic
TCAACATTCACAGGCTCTGG+AGG 0.570563 5:-76824146 None:intergenic
GCTCCTTCATAAAAGCTGCT+TGG 0.572842 5:-76821954 None:intergenic
GGCGGCTGATGCTCATATGT+TGG 0.573754 5:+76821702 MsaT029417.1:CDS
GGAACAGCTGTGGTTGACAT+AGG 0.574547 5:+76825447 MsaT029417.1:CDS
TACCCTTCATTCGGTTGAGG+CGG 0.575922 5:+76821684 MsaT029417.1:CDS
GCCCCTGATGATACAAGGAC+AGG 0.576326 5:+76821781 MsaT029417.1:CDS
AGCCGCCTCAACCGAATGAA+GGG 0.583777 5:-76821686 None:intergenic
CATTCTCCATAAAATTTGGG+AGG 0.584904 5:-76822015 None:intergenic
ATGCCTTTCATAATCAAGAA+GGG 0.589284 5:-76824084 None:intergenic
CATATGTTGGACACAGCTAA+GGG 0.591530 5:+76821715 MsaT029417.1:CDS
TGTCAAAACTGGGAAGGAAG+AGG 0.595392 5:+76821591 MsaT029417.1:CDS
GCCTGTAGATAATGAAAACC+CGG 0.601904 5:+76821858 MsaT029417.1:CDS
AGATGATGTCAAAACTGGGA+AGG 0.611191 5:+76821585 MsaT029417.1:CDS
GAAAACGACAAAGTGGGTCA+GGG 0.611750 5:+76821352 MsaT029417.1:CDS
AAATGAGTCGGGAACAGAAG+AGG 0.612300 5:+76821930 MsaT029417.1:CDS
TAGTCTAGATCAACCCGAAG+AGG 0.623353 5:+76824644 MsaT029417.1:intron
TGCTAGGTCGGGAACAGCTG+TGG 0.627055 5:+76825437 MsaT029417.1:intron
TCCTGTCCTTGTATCATCAG+GGG 0.629902 5:-76821782 None:intergenic
ACAAAAGAAAACGACAAAGT+GGG 0.644226 5:+76821346 MsaT029417.1:CDS
TGAAGATGCTTCTGATGTGA+AGG 0.653690 5:+76821738 MsaT029417.1:CDS
GGCTCTGGAGGAGATGCGAT+AGG 0.659024 5:-76824134 None:intergenic
TCCGGCGAATGTTAATGTAG+AGG 0.660878 5:+76821378 MsaT029417.1:CDS
GAAAGGCATAACATAAAAGG+CGG 0.672469 5:+76824098 MsaT029417.1:CDS
AGATGCTTCTGATGTGAAGG+TGG 0.677639 5:+76821741 MsaT029417.1:CDS
CCGGAAAGTCCAGGAAACTG+TGG 0.685217 5:-76823758 None:intergenic
TGTCAGATGATGTCAAAACT+GGG 0.691161 5:+76821581 MsaT029417.1:CDS
GGATGAAAATGTGAAACCCA+AGG 0.695062 5:+76821624 MsaT029417.1:CDS
GAAGGAAGAGGTGCATGCTG+AGG 0.698279 5:+76821603 MsaT029417.1:CDS
ATAATGCAGTCAAAGCAGCA+CGG 0.717518 5:+76825164 MsaT029417.1:CDS
AGAGCTGTGATGAGATTGGG+AGG 0.730490 5:+76822171 MsaT029417.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 76821337 76825691 76821337 ID=MsaG029417
Chr5 mRNA 76821337 76825691 76821337 ID=MsaT029417.1;Parent=MsaG029417
Chr5 exon 76821337 76822052 76821337 ID=MsaT029417.1.exon1;Parent=MsaT029417.1
Chr5 CDS 76821337 76822052 76821337 ID=cds.MsaT029417.1;Parent=MsaT029417.1
Chr5 exon 76822164 76822232 76822164 ID=MsaT029417.1.exon2;Parent=MsaT029417.1
Chr5 CDS 76822164 76822232 76822164 ID=cds.MsaT029417.1;Parent=MsaT029417.1
Chr5 exon 76823004 76823030 76823004 ID=MsaT029417.1.exon3;Parent=MsaT029417.1
Chr5 CDS 76823004 76823030 76823004 ID=cds.MsaT029417.1;Parent=MsaT029417.1
Chr5 exon 76823750 76823795 76823750 ID=MsaT029417.1.exon4;Parent=MsaT029417.1
Chr5 CDS 76823750 76823795 76823750 ID=cds.MsaT029417.1;Parent=MsaT029417.1
Chr5 exon 76824083 76824175 76824083 ID=MsaT029417.1.exon5;Parent=MsaT029417.1
Chr5 CDS 76824083 76824175 76824083 ID=cds.MsaT029417.1;Parent=MsaT029417.1
Chr5 exon 76824647 76824733 76824647 ID=MsaT029417.1.exon6;Parent=MsaT029417.1
Chr5 CDS 76824647 76824733 76824647 ID=cds.MsaT029417.1;Parent=MsaT029417.1
Chr5 exon 76824976 76825027 76824976 ID=MsaT029417.1.exon7;Parent=MsaT029417.1
Chr5 CDS 76824976 76825027 76824976 ID=cds.MsaT029417.1;Parent=MsaT029417.1
Chr5 exon 76825131 76825206 76825131 ID=MsaT029417.1.exon8;Parent=MsaT029417.1
Chr5 CDS 76825131 76825206 76825131 ID=cds.MsaT029417.1;Parent=MsaT029417.1
Chr5 exon 76825443 76825509 76825443 ID=MsaT029417.1.exon9;Parent=MsaT029417.1
Chr5 CDS 76825443 76825509 76825443 ID=cds.MsaT029417.1;Parent=MsaT029417.1
Chr5 exon 76825596 76825691 76825596 ID=MsaT029417.1.exon10;Parent=MsaT029417.1
Chr5 CDS 76825596 76825691 76825596 ID=cds.MsaT029417.1;Parent=MsaT029417.1
Gene Sequence

>MsaT029417.1

ATGAGTGATACAAAAGAAAACGACAAAGTGGGTCAGGGTGTTCCGGCGAATGTTAATGTAGAGGAAGCTGACCATGTCATTGGTGAAAATGAAAATTCAAAACTAGGAGATGATCATGAGATGATTATTGATAATCTTGCTGGTTTGGAATTAGGAGATGATCATGAGATGATTATTGTTCAAGAGATGCATCTTGAAGTTCAACCGGTTACTGTAGAGGAAACTTCAAAGAATGTTGAAAATGTGTCAGATGATGTCAAAACTGGGAAGGAAGAGGTGCATGCTGAGGATGAAAATGTGAAACCCAAGGTTGTTTCAGAAGGTATTTCACAAGAAAATGTTCAAGATACCCTTCATTCGGTTGAGGCGGCTGATGCTCATATGTTGGACACAGCTAAGGGTGAAGATGCTTCTGATGTGAAGGTGGACGCCAAAAAACTTCCTGCCCCTGATGATACAAGGACAGGAATTAAGAATGTTTTAGAAGTTGAAAGTAAGGACGGTGAAGAGCAAGTTGCTGAGCCTGTAGATAATGAAAACCCGGATTCGCAGCAGAACATGTTTTTCTTGGATGCTGATCATTATTATGATGGAAATGAGTCGGGAACAGAAGAGGACCAAGCAGCTTTTATGAAGGAGCTTGAAAACTTCTTTAGGGAGAGGAGTATGGACTTCAAACCTCCCAAATTTTATGGAGAATGGCTTAATTGCCTTAAGTTATGGAGAGCTGTGATGAGATTGGGAGGCTATGAGAAGGTATTCATTTATTTTTTCCTTTCTATTATTGTGGGAGAGTCTTTCAAACTCCCAAAGACTTGCACCACAGTTTCCTGGACTTTCCGGAGATTTTATGAGAAGGCCCTTCTTGATTATGAAAGGCATAACATAAAAGGCGGCGATCTGAATGTACCTATCGCATCTCCTCCAGAGCCTGTGAATGTTGAAAATCAGTCTAGATCAACCCGAAGAGGTATGCATAAAAGTATTTTCCCCCAAGGCAAAATCCGAGAACTCCTTCTATTTTCTGGGTTTGGAAAAGATAGGAATGCTGTGTCTGTGCAAAAGCGTGAAAAGCAGCTTAAGAGCATCAATGTACCTAAACGTAAGAGCCCATCTTATATGGATAATGCAGTCAAAGCAGCACGGAGTAAACCTTCAAGACCACAGTCGGGAACAGCTGTGGTTGACATAGGACCTTCTGCTGATTGGGTAAAAGTCAATGTTCAAAAAACTAAAGATTGTTTCGAAATATATGCTTTAGTTCCTGGCCTTCTGCGGGAGGAGGTTGGAGAATGCCGAGAAGAAGAAGAAGAAGAAGGAAGCAAGTAA

Protein sequence

>MsaT029417.1

MSDTKENDKVGQGVPANVNVEEADHVIGENENSKLGDDHEMIIDNLAGLELGDDHEMIIVQEMHLEVQPVTVEETSKNVENVSDDVKTGKEEVHAEDENVKPKVVSEGISQENVQDTLHSVEAADAHMLDTAKGEDASDVKVDAKKLPAPDDTRTGIKNVLEVESKDGEEQVAEPVDNENPDSQQNMFFLDADHYYDGNESGTEEDQAAFMKELENFFRERSMDFKPPKFYGEWLNCLKLWRAVMRLGGYEKVFIYFFLSIIVGESFKLPKTCTTVSWTFRRFYEKALLDYERHNIKGGDLNVPIASPPEPVNVENQSRSTRRGMHKSIFPQGKIRELLLFSGFGKDRNAVSVQKREKQLKSINVPKRKSPSYMDNAVKAARSKPSRPQSGTAVVDIGPSADWVKVNVQKTKDCFEIYALVPGLLREEVGECREEEEEEGSK*