Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG029428 | AES61431.2 | 82.883 | 111 | 19 | 0 | 1 | 111 | 81 | 191 | 3.51e-62 | 208 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG029428 | tr|G7I8T1|G7I8T1_MEDTR | 82.883 | 111 | 19 | 0 | 1 | 111 | 81 | 191 | 1.67e-62 | 208 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG029428 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG029428 | MtrunA17_Chr3g0088371 | 74.194 | 93 | 21 | 1 | 185 | 274 | 87 | 179 | 8.53e-39 | 134 |
| MsaG029428 | MtrunA17_Chr1g0172971 | 82.692 | 52 | 9 | 0 | 1 | 52 | 66 | 117 | 7.57e-22 | 94.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTCGCTTCTTTGACCTAAT+TGG | 0.186128 | 5:+77026543 | None:intergenic |
| TTCTTTGACCTAATTGGATT+TGG | 0.299426 | 5:+77026549 | None:intergenic |
| TGTTCTTGAGGCAGGGAAAA+AGG | 0.322946 | 5:-77026697 | MsaT029428.1:CDS |
| GGTTTCGAATGTTGATGTTT+TGG | 0.354913 | 5:-77027109 | MsaT029428.1:CDS |
| CTCAACGGTGTTCTTGAGGC+AGG | 0.386173 | 5:-77026705 | MsaT029428.1:CDS |
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | 5:+77028912 | None:intergenic |
| AAGAAAGACCAAATCCAATT+AGG | 0.397771 | 5:-77026557 | MsaT029428.1:CDS |
| TTTGGTCTTTCTTTGAACTT+TGG | 0.398856 | 5:+77026567 | None:intergenic |
| GAGGAGTTTCTTATTTCTAA+AGG | 0.403538 | 5:-77026672 | MsaT029428.1:CDS |
| GATTATGATCTTCAAACCTT+GGG | 0.413114 | 5:+77027061 | None:intergenic |
| CGATTATGATCTTCAAACCT+TGG | 0.425681 | 5:+77027060 | None:intergenic |
| AGCTGGAATATATTTGAAGT+TGG | 0.433136 | 5:-77028853 | MsaT029428.1:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 5:-77028870 | MsaT029428.1:CDS |
| CGTTATGTTTGTAACAAAGA+AGG | 0.449439 | 5:-77028987 | MsaT029428.1:CDS |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | 5:-77029021 | MsaT029428.1:CDS |
| GGGTTGTCACAGTCGACATT+GGG | 0.480993 | 5:-77027026 | MsaT029428.1:CDS |
| TTACAACCAACCCTTTCCTC+TGG | 0.485330 | 5:+77028876 | None:intergenic |
| CTGTCTGTGAACCATCTGTT+GGG | 0.487793 | 5:-77026596 | MsaT029428.1:CDS |
| CTCAAGTAGCACAAATGCTC+TGG | 0.489977 | 5:+77028765 | None:intergenic |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | 5:-77029027 | MsaT029428.1:CDS |
| TATGAGGAGGACACAACAAA+AGG | 0.490908 | 5:-77027216 | MsaT029428.1:intron |
| GCTGTCTGTGAACCATCTGT+TGG | 0.504047 | 5:-77026597 | MsaT029428.1:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.504865 | 5:+77028777 | None:intergenic |
| ATCATAATCGACTTGATGAA+AGG | 0.507955 | 5:-77027047 | MsaT029428.1:CDS |
| TGTCTATAAGATATATAAGT+GGG | 0.514682 | 5:-77028817 | MsaT029428.1:CDS |
| GATCTTCAAACCTTGGGGAA+AGG | 0.519004 | 5:+77027067 | None:intergenic |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.525236 | 5:-77028976 | MsaT029428.1:CDS |
| TCATAATCGACTTGATGAAA+GGG | 0.526860 | 5:-77027046 | MsaT029428.1:CDS |
| AGGGTTGTCACAGTCGACAT+TGG | 0.538587 | 5:-77027027 | MsaT029428.1:CDS |
| ATGAGGAGGACACAACAAAA+GGG | 0.542065 | 5:-77027215 | MsaT029428.1:intron |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 5:-77028975 | MsaT029428.1:CDS |
| ATGTCTATAAGATATATAAG+TGG | 0.572287 | 5:-77028818 | MsaT029428.1:CDS |
| TTTGGTGCAGTCCCAACAGA+TGG | 0.581566 | 5:+77026585 | None:intergenic |
| TCAACGGTGTTCTTGAGGCA+GGG | 0.590097 | 5:-77026704 | MsaT029428.1:CDS |
| ATTTCTCAACGGTGTTCTTG+AGG | 0.591378 | 5:-77026709 | MsaT029428.1:CDS |
| TTGAAGAAAACCTTTCCCCA+AGG | 0.594796 | 5:-77027077 | MsaT029428.1:CDS |
| TGAAGACTTAGAAGGCGTAA+CGG | 0.598405 | 5:+77026629 | None:intergenic |
| GAAGCAAAAGAAAGATGATG+AGG | 0.601308 | 5:-77026467 | MsaT029428.1:CDS |
| TCGTGTTTGCGCAGATGCGG+AGG | 0.605841 | 5:-77026742 | MsaT029428.1:intron |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | 5:+77028927 | None:intergenic |
| TATAAGATATATAAGTGGGA+AGG | 0.626658 | 5:-77028813 | MsaT029428.1:CDS |
| ATTATGATCTTCAAACCTTG+GGG | 0.630358 | 5:+77027062 | None:intergenic |
| GCATCTGAAATATTTCTCAA+CGG | 0.661104 | 5:-77026720 | MsaT029428.1:CDS |
| ATGTTGATAATGATAATGAG+AGG | 0.683310 | 5:-77027131 | MsaT029428.1:CDS |
| TGTTGATAATGATAATGAGA+GGG | 0.689129 | 5:-77027130 | MsaT029428.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 77026461 | 77029066 | 77026461 | ID=MsaG029428 |
| Chr5 | mRNA | 77026461 | 77029066 | 77026461 | ID=MsaT029428.1;Parent=MsaG029428 |
| Chr5 | exon | 77026461 | 77026750 | 77026461 | ID=MsaT029428.1.exon3;Parent=MsaT029428.1 |
| Chr5 | CDS | 77026461 | 77026750 | 77026461 | ID=cds.MsaT029428.1;Parent=MsaT029428.1 |
| Chr5 | exon | 77027001 | 77027232 | 77027001 | ID=MsaT029428.1.exon2;Parent=MsaT029428.1 |
| Chr5 | CDS | 77027001 | 77027232 | 77027001 | ID=cds.MsaT029428.1;Parent=MsaT029428.1 |
| Chr5 | exon | 77028752 | 77029066 | 77028752 | ID=MsaT029428.1.exon1;Parent=MsaT029428.1 |
| Chr5 | CDS | 77028752 | 77029066 | 77028752 | ID=cds.MsaT029428.1;Parent=MsaT029428.1 |
| Gene Sequence |
| Protein sequence |
