Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG029434 | XP_039687742.1 | 86.957 | 92 | 9 | 1 | 1 | 92 | 1 | 89 | 6.27e-39 | 143 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG029434 | sp|O80982|HSFA2_ARATH | 73.684 | 57 | 15 | 0 | 36 | 92 | 31 | 87 | 5.61e-26 | 101 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG029434 | tr|G7J7P1|G7J7P1_MEDTR | 86.957 | 92 | 9 | 1 | 1 | 92 | 1 | 89 | 2.99e-39 | 143 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG029434 | TF | HSF |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG029434 | MtrunA17_Chr3g0135831 | 86.957 | 92 | 9 | 1 | 1 | 92 | 1 | 89 | 5.77e-43 | 143 |
| MsaG029434 | MtrunA17_Chr7g0260331 | 55.172 | 87 | 38 | 1 | 6 | 92 | 7 | 92 | 1.89e-24 | 94.7 |
| MsaG029434 | MtrunA17_Chr4g0071051 | 60.870 | 69 | 27 | 0 | 24 | 92 | 76 | 144 | 4.24e-24 | 94.4 |
| MsaG029434 | MtrunA17_Chr1g0204741 | 62.121 | 66 | 25 | 0 | 27 | 92 | 18 | 83 | 2.17e-23 | 91.3 |
| MsaG029434 | MtrunA17_Chr1g0185131 | 63.158 | 57 | 21 | 0 | 36 | 92 | 19 | 75 | 5.65e-19 | 79.7 |
| MsaG029434 | MtrunA17_Chr5g0410881 | 54.386 | 57 | 24 | 1 | 36 | 92 | 21 | 75 | 1.72e-15 | 70.1 |
| MsaG029434 | MtrunA17_Chr1g0179321 | 53.448 | 58 | 27 | 0 | 35 | 92 | 35 | 92 | 2.59e-14 | 63.2 |
| MsaG029434 | MtrunA17_Chr2g0284481 | 55.556 | 45 | 20 | 0 | 48 | 92 | 14 | 58 | 9.52e-12 | 59.7 |
| MsaG029434 | MtrunA17_Chr2g0330821 | 52.000 | 50 | 24 | 0 | 43 | 92 | 73 | 122 | 1.40e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG029434 | AT2G26150.1 | 73.684 | 57 | 15 | 0 | 36 | 92 | 31 | 87 | 5.71e-27 | 101 |
| MsaG029434 | AT2G26150.4 | 73.684 | 57 | 15 | 0 | 36 | 92 | 31 | 87 | 5.71e-27 | 101 |
| MsaG029434 | AT3G51910.1 | 68.519 | 54 | 17 | 0 | 39 | 92 | 19 | 72 | 1.70e-21 | 85.9 |
| MsaG029434 | AT3G22830.1 | 61.404 | 57 | 22 | 0 | 36 | 92 | 48 | 104 | 3.60e-21 | 86.3 |
| MsaG029434 | AT3G63350.1 | 60.294 | 68 | 27 | 0 | 25 | 92 | 4 | 71 | 5.83e-19 | 79.3 |
| MsaG029434 | AT4G17750.1 | 63.043 | 46 | 17 | 0 | 47 | 92 | 50 | 95 | 9.27e-15 | 68.6 |
| MsaG029434 | AT5G43840.1 | 53.571 | 56 | 26 | 0 | 36 | 91 | 6 | 61 | 1.59e-13 | 64.7 |
| MsaG029434 | AT3G02990.1 | 55.556 | 45 | 20 | 0 | 48 | 92 | 22 | 66 | 9.48e-13 | 62.8 |
| MsaG029434 | AT1G32330.2 | 41.558 | 77 | 45 | 0 | 15 | 91 | 3 | 79 | 1.29e-12 | 62.4 |
| MsaG029434 | AT1G32330.1 | 41.558 | 77 | 45 | 0 | 15 | 91 | 3 | 79 | 1.33e-12 | 62.4 |
| MsaG029434 | AT5G54070.1 | 51.923 | 52 | 24 | 1 | 42 | 92 | 63 | 114 | 2.79e-12 | 61.2 |
| MsaG029434 | AT5G16820.1 | 55.556 | 45 | 20 | 0 | 48 | 92 | 26 | 70 | 6.23e-12 | 60.5 |
| MsaG029434 | AT5G16820.2 | 55.556 | 45 | 20 | 0 | 48 | 92 | 26 | 70 | 6.23e-12 | 60.5 |
| MsaG029434 | AT5G03720.2 | 44.000 | 75 | 41 | 1 | 17 | 91 | 24 | 97 | 1.38e-11 | 59.7 |
| MsaG029434 | AT5G03720.1 | 44.000 | 75 | 41 | 1 | 17 | 91 | 24 | 97 | 1.38e-11 | 59.7 |
Find 22 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAACAACATCAAAGGTTT+TGG | 0.271463 | 5:+77107009 | None:intergenic |
| TGTAACCCTTCCATTGGTTT+TGG | 0.288353 | 5:+77107058 | None:intergenic |
| AACTCTTCATCTTCCTCTTC+GGG | 0.307939 | 5:-77107094 | MsaT029434.1:CDS |
| TAACTCTTCATCTTCCTCTT+CGG | 0.322245 | 5:-77107095 | MsaT029434.1:CDS |
| AATGGAAGGGTTACATGAAA+TGG | 0.353101 | 5:-77107050 | MsaT029434.1:CDS |
| TTAGCATATGTCAGTGTTGT+TGG | 0.370710 | 5:-77107130 | MsaT029434.1:CDS |
| GAAGGGAATGAGAGTGAAAG+AGG | 0.458201 | 5:-77107161 | MsaT029434.1:CDS |
| ATTTCATGTAACCCTTCCAT+TGG | 0.467390 | 5:+77107052 | None:intergenic |
| ATGGAAGGGTTACATGAAAT+GGG | 0.489232 | 5:-77107049 | MsaT029434.1:CDS |
| ACAACATGTTAGAAGAAGAA+AGG | 0.539604 | 5:+77106885 | None:intergenic |
| GATTCCATTGTTTCATTGAG+CGG | 0.558045 | 5:-77106974 | MsaT029434.1:CDS |
| TGGATCTTCAACAACATCAA+AGG | 0.559140 | 5:+77107003 | None:intergenic |
| AAATCTTACACCAAAACCAA+TGG | 0.575232 | 5:-77107068 | MsaT029434.1:CDS |
| CAATGAAACAATGGAATCAG+TGG | 0.583950 | 5:+77106979 | None:intergenic |
| GAAACAATGGAATCAGTGGA+TGG | 0.595303 | 5:+77106983 | None:intergenic |
| CTTACACCAAAACCAATGGA+AGG | 0.595875 | 5:-77107064 | MsaT029434.1:CDS |
| AGCATCAACTACTCAATGAA+GGG | 0.599719 | 5:-77107178 | None:intergenic |
| TGTAAGATTTGAACCCGAAG+AGG | 0.623342 | 5:+77107081 | None:intergenic |
| AGCTCCGCTCAATGAAACAA+TGG | 0.624284 | 5:+77106970 | None:intergenic |
| TTACACCAAAACCAATGGAA+GGG | 0.647911 | 5:-77107063 | MsaT029434.1:CDS |
| GTAACGAGGTAAAATAGCAG+TGG | 0.662321 | 5:+77106910 | None:intergenic |
| GAAGAAGAAAGGTCGTAACG+AGG | 0.715157 | 5:+77106896 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 77106883 | 77107185 | 77106883 | ID=MsaG029434 |
| Chr5 | mRNA | 77106883 | 77107185 | 77106883 | ID=MsaT029434.1;Parent=MsaG029434 |
| Chr5 | exon | 77106883 | 77107185 | 77106883 | ID=MsaT029434.1.exon1;Parent=MsaT029434.1 |
| Chr5 | CDS | 77106883 | 77107185 | 77106883 | ID=cds.MsaT029434.1;Parent=MsaT029434.1 |
| Gene Sequence |
| Protein sequence |
