AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029533

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029533 MtrunA17_Chr5g0444961 97.655 725 17 0 1 725 1 725 0.0 1455
MsaG029533 MtrunA17_Chr5g0444951 100.000 104 0 0 726 829 1 104 5.10e-71 228
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029533 AT1G50620.1 36.770 514 243 13 3 510 2 439 2.02e-84 282
MsaG029533 AT3G20280.4 34.937 478 217 14 19 491 19 407 2.63e-68 241
MsaG029533 AT3G20280.1 34.937 478 217 14 19 491 19 407 2.63e-68 241
MsaG029533 AT3G20280.5 35.146 478 215 14 19 491 19 406 9.97e-68 239
MsaG029533 AT3G20280.3 35.146 478 215 14 19 491 19 406 9.97e-68 239
MsaG029533 AT3G20280.2 43.704 135 74 2 358 491 13 146 3.39e-34 137

Find 216 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCATCACACGGCCATATTT+TGG 0.179992 5:+78417884 None:intergenic
AACAAACCTGCAAAGGAAAA+TGG 0.206689 5:+78415572 None:intergenic
GCTTTGGTATTAACAACTTC+TGG 0.239867 5:+78418140 None:intergenic
GCTGAATTAGAGGGAAAATT+TGG 0.254184 5:+78417845 None:intergenic
CCGAATTCTGTTAGAGGTTC+TGG 0.265010 5:-78417284 MsaT029533.1:CDS
AGATTCAGAAGTATCTTTAC+AGG 0.287346 5:+78417520 None:intergenic
TACCAATCTTGCTGCATTGA+TGG 0.287633 5:-78417158 MsaT029533.1:CDS
AACCCACTTCACCCCAGTTT+TGG 0.292600 5:+78416643 None:intergenic
CCTTGTATATCTCTCGTATC+TGG 0.292839 5:+78417680 None:intergenic
TACTATGCACCAGCCGTTGC+AGG 0.304496 5:-78418221 MsaT029533.1:CDS
TTAGCCAGCTCAGATATATT+AGG 0.314993 5:+78417488 None:intergenic
AAGCCTTTGCCTCCAAAATA+TGG 0.319020 5:-78417896 MsaT029533.1:CDS
TGCTGAACAAATAGATTCTA+AGG 0.324390 5:-78417549 MsaT029533.1:CDS
CTACTTTCCTGATGATTTGC+CGG 0.326963 5:-78416606 MsaT029533.1:CDS
ATGTGGGAAGATTCCAAAAC+TGG 0.327053 5:-78416656 MsaT029533.1:CDS
TGGGGCTCCTTATGTCCTTC+AGG 0.331452 5:-78419083 MsaT029533.1:CDS
ACCTTCGGATCTAAGTTATC+TGG 0.333379 5:+78417794 None:intergenic
GCAGATTTCACACATATGTT+TGG 0.346253 5:+78417611 None:intergenic
CTAAACTGTAGCAAATTCAT+TGG 0.350286 5:-78418455 MsaT029533.1:intron
AATGACAGAAGATGTCAATT+TGG 0.360459 5:-78415621 MsaT029533.1:CDS
TTGCTTGCCTGAAGTTTAGA+AGG 0.364469 5:+78417077 None:intergenic
GCCGTTGCAGGGTGGGAATA+TGG 0.364784 5:-78418209 MsaT029533.1:CDS
TTGAATACCAGCTGAATTAG+AGG 0.365719 5:+78417835 None:intergenic
TCTGGTTTCTTCTCTGAAGA+TGG 0.371119 5:+78417812 None:intergenic
GCACAACCGAATTCTGTTAG+AGG 0.372523 5:-78417290 MsaT029533.1:CDS
GATGATTTGCCGGGGAATAT+TGG 0.377341 5:-78416596 MsaT029533.1:CDS
CTCCATCAATGCAGCAAGAT+TGG 0.380179 5:+78417156 None:intergenic
TCTTGTTTAAATTTATCATA+TGG 0.381248 5:+78415645 None:intergenic
AAGGTTGTGGATCAGAGGTT+AGG 0.385332 5:-78420389 MsaT029533.1:CDS
CCAGAACCTCTAACAGAATT+CGG 0.389101 5:+78417284 None:intergenic
TAACAGCAGCTCAGAACATT+AGG 0.389751 5:-78418279 MsaT029533.1:CDS
ACTAACACCTTCTAAACTTC+AGG 0.394509 5:-78417084 MsaT029533.1:intron
CATCAGTGAATGCTCCTTCA+TGG 0.395283 5:-78418423 MsaT029533.1:CDS
AGGTGTTAGTTTGCCATGGC+TGG 0.400222 5:+78417097 None:intergenic
GGAGTCCATGTGGGATGATC+AGG 0.400694 5:+78418115 None:intergenic
TCCACCGGTGTTTGTTTGAA+AGG 0.401303 5:+78419381 None:intergenic
CTCTGTGATGCCTGTGAAAA+GGG 0.404413 5:-78418013 MsaT029533.1:CDS
CCTTATGTCCTTCAGGTTCA+AGG 0.407142 5:-78419076 MsaT029533.1:intron
TGGATCAGGCTTCTCGTCAA+TGG 0.408193 5:+78417631 None:intergenic
GGTGGAGGAGAAAGAGAATT+CGG 0.409063 5:-78420621 MsaT029533.1:CDS
CCATTTGTTGTTAACATCTG+TGG 0.410839 5:+78417767 None:intergenic
GCAGTTCCTTCAACCTTGAC+AGG 0.411352 5:+78418334 None:intergenic
CAGACTCAACCTGTGTCATT+AGG 0.411643 5:-78418394 MsaT029533.1:CDS
TTCACACATATGTTTGGATC+AGG 0.414156 5:+78417617 None:intergenic
TGCATAGTAGACATATTTCC+AGG 0.419884 5:+78418235 None:intergenic
AAGGAGCATTCACTGATGAT+TGG 0.420539 5:+78418428 None:intergenic
TAATAGTGATAGAGTTGAAA+TGG 0.426583 5:-78415700 MsaT029533.1:CDS
ACGAGTGGCGGAGATTGTTT+TGG 0.427221 5:-78420585 MsaT029533.1:CDS
CCACCGGTGTTTGTTTGAAA+GGG 0.428372 5:+78419382 None:intergenic
CTAGGCAATGCTACAGAAGA+TGG 0.430376 5:+78419145 None:intergenic
GGTAGTGTGATTGATGATCT+TGG 0.433796 5:-78420422 MsaT029533.1:CDS
TACTTTCCTGATGATTTGCC+GGG 0.434354 5:-78416605 MsaT029533.1:CDS
GTGGGATGATCAGGAAGCTT+TGG 0.435804 5:+78418124 None:intergenic
CTTTATTATTAGTAATCAAA+AGG 0.437685 5:-78417369 MsaT029533.1:CDS
TGGCACTGCATGAAGTGCTT+GGG 0.438105 5:-78417932 MsaT029533.1:CDS
AACATCTGTGGGCTGACCTT+CGG 0.439150 5:+78417779 None:intergenic
GACACAGATCAACTGATAAC+AGG 0.446298 5:+78412259 None:intergenic
CTTGTATATCTCTCGTATCT+GGG 0.448811 5:+78417681 None:intergenic
CATAACCCTGTCAAGGTTGA+AGG 0.449038 5:-78418340 MsaT029533.1:CDS
TAGGCCATTCATTACTCAAA+CGG 0.450525 5:-78418260 MsaT029533.1:CDS
TGCAGGGTGGGAATATGGTT+CGG 0.450729 5:-78418204 MsaT029533.1:CDS
AACTGGGGTGAAGTGGGTTA+AGG 0.454696 5:-78416639 MsaT029533.1:CDS
CCACAGATGTTAACAACAAA+TGG 0.455029 5:-78417767 MsaT029533.1:CDS
CTGCTGATGCAACCGTATCA+AGG 0.456875 5:-78418315 MsaT029533.1:CDS
CTCAGTATTGTGATTGGTAC+TGG 0.457635 5:+78417724 None:intergenic
ATTGTTGGTGGTGAAGCTAT+TGG 0.458790 5:-78420443 MsaT029533.1:CDS
TGAGATGTATATGTTGTAGA+AGG 0.461719 5:+78419406 None:intergenic
AATGGAAACTCTGTTCCAAC+TGG 0.462215 5:-78417749 MsaT029533.1:CDS
AGGCCATTCATTACTCAAAC+GGG 0.462455 5:-78418259 MsaT029533.1:CDS
GGCATTACACCAGAAACAAT+GGG 0.462964 5:+78419190 None:intergenic
ACAAACCTGCAAAGGAAAAT+GGG 0.463372 5:+78415573 None:intergenic
AGAAGATCCTGCAGCAAGAT+GGG 0.463622 5:+78419255 None:intergenic
AAAATTTGGTGATGTAATTG+AGG 0.464766 5:+78417859 None:intergenic
ATATTCCCCGGCAAATCATC+AGG 0.467697 5:+78416599 None:intergenic
CCTTGAACCTGAAGGACATA+AGG 0.468335 5:+78419076 None:intergenic
ATGCCTCCCCATCTTGCTGC+AGG 0.468712 5:-78419262 MsaT029533.1:CDS
AGGTTGTGGATCAGAGGTTA+GGG 0.469590 5:-78420388 MsaT029533.1:CDS
TTGGCACTGCATGAAGTGCT+TGG 0.469894 5:-78417933 MsaT029533.1:CDS
TCTCTGTGATGCCTGTGAAA+AGG 0.471396 5:-78418014 MsaT029533.1:CDS
TTCTGAGCTGCTGTTACTTG+TGG 0.472825 5:+78418286 None:intergenic
AACATTCGAACAGCAGTTGT+TGG 0.473666 5:+78419340 None:intergenic
TTCATTACTCAAACGGGACC+TGG 0.476773 5:-78418253 MsaT029533.1:CDS
CTCTCCGATGGAGGACGTGC+CGG 0.485673 5:-78420729 None:intergenic
GAATTCATAATAGAGTTGAT+TGG 0.486542 5:-78417952 MsaT029533.1:CDS
TTTCTCCCCATGTGACTGCT+AGG 0.489377 5:+78419169 None:intergenic
GAGAGAAGCGAGGACGAAGC+TGG 0.490332 5:-78420498 MsaT029533.1:CDS
CATTTGTTGTTAACATCTGT+GGG 0.495351 5:+78417768 None:intergenic
CTCTAATGACTGATGGCTGC+AGG 0.498012 5:+78417975 None:intergenic
CTGCAGCCATCAGTCATTAG+AGG 0.498511 5:-78417974 MsaT029533.1:CDS
TATTCCCACCCTGCAACGGC+TGG 0.501073 5:+78418212 None:intergenic
ATAGCTCACATGCTGTAGAA+TGG 0.501093 5:-78417220 MsaT029533.1:CDS
TTACTCATATAATCTCTTGA+CGG 0.504323 5:+78418091 None:intergenic
ACCTTGACAGGGTTATGAGC+AGG 0.504718 5:+78418346 None:intergenic
CCACAGTTTAAAGTTACTGC+AGG 0.508542 5:-78419112 MsaT029533.1:CDS
CAGAAGATCCTGCAGCAAGA+TGG 0.511296 5:+78419254 None:intergenic
CTGCAGTAACTTTAAACTGT+GGG 0.511405 5:+78419113 None:intergenic
TTCTCAGCAATCGACATCTT+CGG 0.512043 5:+78420356 None:intergenic
TTGTGGACCTGCCCTTGATA+CGG 0.512083 5:+78418303 None:intergenic
AAGTTACTGCAGGATTGAGT+GGG 0.513481 5:-78419102 MsaT029533.1:CDS
GTTTAAATTTATCATATGGA+AGG 0.514272 5:+78415649 None:intergenic
AGTTGAGAATGCAGTGTCAC+AGG 0.514317 5:-78417432 MsaT029533.1:CDS
TGGATTGAATGCTAAGGTTG+TGG 0.515077 5:-78420402 MsaT029533.1:CDS
AGCACAGTGCTGAATACCTT+CGG 0.519436 5:+78417253 None:intergenic
GAGCAAGAAGGCGAAGGTGG+AGG 0.520743 5:-78420636 MsaT029533.1:CDS
TTCAATGCTAGATGAAATGT+TGG 0.520996 5:+78417655 None:intergenic
AGCTCAACCTCAACATCAGT+TGG 0.521719 5:+78420524 None:intergenic
TCAAACAAACACCGGTGGAA+TGG 0.521960 5:-78419377 MsaT029533.1:CDS
AATTCGGTTGTGCAGCATCT+AGG 0.523508 5:+78417300 None:intergenic
GACGCCATGGAAACTGTAGA+AGG 0.525946 5:+78419286 None:intergenic
TGCACCAGCCGTTGCAGGGT+GGG 0.526817 5:-78418216 MsaT029533.1:CDS
CCGCCACCGGAGATTGAAAA+CGG 0.533523 5:-78420686 MsaT029533.1:CDS
GTTGAGCAAGAAGGCGAAGG+TGG 0.534577 5:-78420639 MsaT029533.1:CDS
TCTTCCTAATATATCTGAGC+TGG 0.534609 5:-78417492 MsaT029533.1:CDS
AATGCCTTCTACAGTTTCCA+TGG 0.538341 5:-78419290 MsaT029533.1:CDS
TAGAAGGTGTTAGTTTGCCA+TGG 0.541388 5:+78417093 None:intergenic
ATGTCAAGGGATTGCTCCGA+AGG 0.543374 5:-78420468 MsaT029533.1:CDS
ATTCCAAAACTGGGGTGAAG+TGG 0.544447 5:-78416646 MsaT029533.1:CDS
ACCATATTCCCACCCTGCAA+CGG 0.548347 5:+78418208 None:intergenic
TCCACTGGCGTGCAGAGTGA+AGG 0.548439 5:-78417575 MsaT029533.1:CDS
AGGCATTACACCAGAAACAA+TGG 0.552320 5:+78419189 None:intergenic
GCATTCTCAACTTGAGAACC+TGG 0.556429 5:+78417443 None:intergenic
AAAGTTACTGCAGGATTGAG+TGG 0.556480 5:-78419103 MsaT029533.1:CDS
GCGGTTGAGCAAGAAGGCGA+AGG 0.557032 5:-78420642 MsaT029533.1:CDS
AAGATGTCAATTTGGAGTTG+AGG 0.557635 5:-78415613 MsaT029533.1:CDS
TCACCACCAACAATATCCTT+CGG 0.558219 5:+78420452 None:intergenic
TTCCAAAACTGGGGTGAAGT+GGG 0.558948 5:-78416645 MsaT029533.1:CDS
GGGGTATTTGAGTCAAATGA+TGG 0.563344 5:+78417701 None:intergenic
ATTGCTCCGAAGGATATTGT+TGG 0.566615 5:-78420458 MsaT029533.1:CDS
ACCAGATAACTTAGATCCGA+AGG 0.568578 5:-78417795 MsaT029533.1:CDS
TCAGAGTGACTAGGTATCAA+AGG 0.568771 5:+78418178 None:intergenic
TGCTGATGCAACCGTATCAA+GGG 0.569247 5:-78418314 MsaT029533.1:CDS
ACCGACGCTGGATCCGCCAC+CGG 0.570521 5:-78420699 MsaT029533.1:CDS
TGTAATGCCTAGCAGTCACA+TGG 0.570579 5:-78419176 MsaT029533.1:CDS
ACCTTCACTCTGCACGCCAG+TGG 0.571113 5:+78417574 None:intergenic
AGAGGAGCGGTTGAGCAAGA+AGG 0.576752 5:-78420648 MsaT029533.1:CDS
AAGCTGGCGGTACTATGTCA+AGG 0.578849 5:-78420482 MsaT029533.1:CDS
AAGTGCTTGGGTTTAAGCGG+TGG 0.579160 5:-78417920 MsaT029533.1:CDS
TCCGGTGGCGGATCCAGCGT+CGG 0.579997 5:+78420698 None:intergenic
GTATTTGTTCGCAAAATCAA+AGG 0.580117 5:-78420333 MsaT029533.1:intron
CGGAGAAGGAGAGCAACAAG+AGG 0.580490 5:-78420666 MsaT029533.1:CDS
AATCAAAAGGAATCTCATGA+AGG 0.581954 5:-78417356 MsaT029533.1:CDS
TGATGGCTCAGTATTGTGAT+TGG 0.583404 5:+78417718 None:intergenic
CCTGCAGTAACTTTAAACTG+TGG 0.583662 5:+78419112 None:intergenic
TGAATTCCTCTAATGACTGA+TGG 0.584828 5:+78417968 None:intergenic
CAGTTCCTTCAACCTTGACA+GGG 0.584947 5:+78418335 None:intergenic
GAAGGACTACACAAGGGCCT+CGG 0.586318 5:+78415667 None:intergenic
ATGGGGAGGCATAGACGCCA+TGG 0.586488 5:+78419273 None:intergenic
GATGCAACCGTATCAAGGGC+AGG 0.587331 5:-78418310 MsaT029533.1:CDS
GCAGCTTGTAGTGCTTCCAC+TGG 0.588290 5:-78417590 MsaT029533.1:CDS
ATAAAGAAACAAACCTGCAA+AGG 0.590380 5:+78415565 None:intergenic
ATGCTAAGGTTGTGGATCAG+AGG 0.590563 5:-78420394 MsaT029533.1:CDS
ACTATGCACCAGCCGTTGCA+GGG 0.591052 5:-78418220 MsaT029533.1:CDS
GCATTGATGGAGTAACATAT+AGG 0.592756 5:-78417145 MsaT029533.1:CDS
TCCAGCGTCGGTTCTGACAC+CGG 0.594083 5:+78420710 None:intergenic
GCTCCGAAGGATATTGTTGG+TGG 0.596231 5:-78420455 MsaT029533.1:CDS
GACACCGGCACGTCCTCCAT+CGG 0.596510 5:+78420725 None:intergenic
GGAGAAAGAGAATTCGGAGG+CGG 0.597927 5:-78420615 MsaT029533.1:CDS
CCGGAGATTGAAAACGGAGA+AGG 0.600320 5:-78420680 MsaT029533.1:CDS
TGTGGGAAGATTCCAAAACT+GGG 0.603012 5:-78416655 MsaT029533.1:CDS
CAAGGATGACCAATATTCCC+CGG 0.605167 5:+78416587 None:intergenic
GGTCCCGTTTGAGTAATGAA+TGG 0.605708 5:+78418256 None:intergenic
GATCCTGCAGCAAGATGGGG+AGG 0.607645 5:+78419259 None:intergenic
TGAATACCAGCTGAATTAGA+GGG 0.607918 5:+78417836 None:intergenic
CTGGTTGATTGATATTGCGA+TGG 0.607966 5:+78419229 None:intergenic
GCCGGTGTCAGAACCGACGC+TGG 0.611187 5:-78420711 MsaT029533.1:CDS
GGAGTAACATATAGGCTGCA+AGG 0.611649 5:-78417137 MsaT029533.1:CDS
TCACATGCTGTAGAATGGAT+TGG 0.614992 5:-78417215 MsaT029533.1:CDS
GCCTGCTCATAACCCTGTCA+AGG 0.615629 5:-78418347 MsaT029533.1:CDS
TGATCTTGGATTGAATGCTA+AGG 0.619045 5:-78420408 MsaT029533.1:CDS
ACGATTGTCAACCATTCCAC+CGG 0.621253 5:+78419366 None:intergenic
CCCTTTCAAACAAACACCGG+TGG 0.624793 5:-78419382 MsaT029533.1:CDS
AGCTGGCGGTACTATGTCAA+GGG 0.626107 5:-78420481 MsaT029533.1:CDS
GGAAATTACCTTGAACCTGA+AGG 0.627294 5:+78419068 None:intergenic
AGCTTCCTGATCATCCCACA+TGG 0.628655 5:-78418120 MsaT029533.1:CDS
TAGTCACTCTGATATTGCAA+AGG 0.630150 5:-78418167 MsaT029533.1:CDS
ACGATGGCGACGATGCGAAG+TGG 0.630685 5:-78420554 MsaT029533.1:CDS
GAAGATCCTGCAGCAAGATG+GGG 0.631370 5:+78419256 None:intergenic
TTGTATATCTCTCGTATCTG+GGG 0.631520 5:+78417682 None:intergenic
TAGAAATGTATCAGAAAACA+AGG 0.631821 5:-78418371 MsaT029533.1:CDS
TCATATGGAAGGACTACACA+AGG 0.639113 5:+78415660 None:intergenic
CCAGATACGAGAGATATACA+AGG 0.639717 5:-78417680 MsaT029533.1:CDS
GGTTCTGGAACTAATACCGA+AGG 0.643550 5:-78417269 MsaT029533.1:CDS
CAACCCTTTCAAACAAACAC+CGG 0.644891 5:-78419385 MsaT029533.1:CDS
ATGCACCAGCCGTTGCAGGG+TGG 0.646972 5:-78418217 MsaT029533.1:CDS
AAGGAGAGCAACAAGAGGAG+CGG 0.647384 5:-78420661 MsaT029533.1:CDS
TGAGTCTGTATTGACCATGA+AGG 0.649233 5:+78418409 None:intergenic
GGAGGAGAAAGAGAATTCGG+AGG 0.649895 5:-78420618 MsaT029533.1:CDS
GGAAATTTACAATCATCACC+AGG 0.650143 5:-78417461 MsaT029533.1:CDS
TTGAGCTGATGAGAGAAGCG+AGG 0.651450 5:-78420508 MsaT029533.1:CDS
AAAGAAGCCAACTGATGTTG+AGG 0.654128 5:-78420531 MsaT029533.1:CDS
ACATTTCTACCTAATGACAC+AGG 0.655198 5:+78418385 None:intergenic
TAATTGAGGATCGCATCACA+CGG 0.655580 5:+78417873 None:intergenic
CTGTAGAAGGCATTGATGTG+TGG 0.656663 5:+78419299 None:intergenic
ATGAAGTGCTTGGGTTTAAG+CGG 0.659398 5:-78417923 MsaT029533.1:CDS
ACTTTCCTGATGATTTGCCG+GGG 0.664009 5:-78416604 MsaT029533.1:CDS
TCGTTGACTTCAGATATACA+AGG 0.666673 5:+78416569 None:intergenic
CTTGACGGAGGAGTCCATGT+GGG 0.667564 5:+78418106 None:intergenic
GGCGGAATTGAAACGAGTGG+CGG 0.668581 5:-78420597 MsaT029533.1:CDS
AGTTACTGCAGGATTGAGTG+GGG 0.674228 5:-78419101 MsaT029533.1:CDS
TCTTGACGGAGGAGTCCATG+TGG 0.677184 5:+78418105 None:intergenic
GTAATGCCTAGCAGTCACAT+GGG 0.677357 5:-78419175 MsaT029533.1:CDS
CTCATATAATCTCTTGACGG+AGG 0.678019 5:+78418094 None:intergenic
TTTGCAATATCAGAGTGACT+AGG 0.684493 5:+78418169 None:intergenic
GGAGGCGGAATTGAAACGAG+TGG 0.684533 5:-78420600 MsaT029533.1:CDS
GCATTACACCAGAAACAATG+GGG 0.686510 5:+78419191 None:intergenic
GAAATGGCTAGTTCTATCCG+AGG 0.698577 5:-78415684 MsaT029533.1:CDS
AGAAGCGAGGACGAAGCTGG+CGG 0.699972 5:-78420495 MsaT029533.1:CDS
TAATGCCTAGCAGTCACATG+GGG 0.703271 5:-78419174 MsaT029533.1:CDS
GAGTGACTAGGTATCAAAGG+AGG 0.704439 5:+78418181 None:intergenic
CATTACACCAGAAACAATGG+GGG 0.706770 5:+78419192 None:intergenic
GTGGGAAGATTCCAAAACTG+GGG 0.708638 5:-78416654 MsaT029533.1:CDS
TGATTGGTACTGGAACCAGT+TGG 0.722677 5:+78417734 None:intergenic
GTGTGAGCTCACTGTCAGTG+AGG 0.735809 5:-78418050 MsaT029533.1:CDS
CATATGGAAGGACTACACAA+GGG 0.743406 5:+78415661 None:intergenic
TTGTATATCTGAAGTCAACG+AGG 0.762476 5:-78416567 MsaT029533.1:intron
ATTACACCAGAAACAATGGG+GGG 0.773168 5:+78419193 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 78412267 78420744 78412267 ID=MsaG029533
Chr5 mRNA 78412267 78420744 78412267 ID=MsaT029533.1;Parent=MsaG029533
Chr5 exon 78412267 78412315 78412267 ID=MsaT029533.1.exon6;Parent=MsaT029533.1
Chr5 CDS 78412267 78412315 78412267 ID=cds.MsaT029533.1;Parent=MsaT029533.1
Chr5 exon 78415579 78415733 78415579 ID=MsaT029533.1.exon5;Parent=MsaT029533.1
Chr5 CDS 78415579 78415733 78415579 ID=cds.MsaT029533.1;Parent=MsaT029533.1
Chr5 exon 78416568 78416681 78416568 ID=MsaT029533.1.exon4;Parent=MsaT029533.1
Chr5 CDS 78416568 78416681 78416568 ID=cds.MsaT029533.1;Parent=MsaT029533.1
Chr5 exon 78417085 78418466 78417085 ID=MsaT029533.1.exon3;Parent=MsaT029533.1
Chr5 CDS 78417085 78418466 78417085 ID=cds.MsaT029533.1;Parent=MsaT029533.1
Chr5 exon 78419077 78419455 78419077 ID=MsaT029533.1.exon2;Parent=MsaT029533.1
Chr5 CDS 78419077 78419455 78419077 ID=cds.MsaT029533.1;Parent=MsaT029533.1
Chr5 exon 78420334 78420744 78420334 ID=MsaT029533.1.exon1;Parent=MsaT029533.1
Chr5 CDS 78420334 78420744 78420334 ID=cds.MsaT029533.1;Parent=MsaT029533.1
Gene Sequence

>MsaT029533.1

ATGGAGGACGTGCCGGTGTCAGAACCGACGCTGGATCCGCCACCGGAGATTGAAAACGGAGAAGGAGAGCAACAAGAGGAGCGGTTGAGCAAGAAGGCGAAGGTGGAGGAGAAAGAGAATTCGGAGGCGGAATTGAAACGAGTGGCGGAGATTGTTTTGGTGTTATCGACGATGGCGACGATGCGAAGTGGAAAGAAGCCAACTGATGTTGAGGTTGAGCTGATGAGAGAAGCGAGGACGAAGCTGGCGGTACTATGTCAAGGGATTGCTCCGAAGGATATTGTTGGTGGTGAAGCTATTGGTAGTGTGATTGATGATCTTGGATTGAATGCTAAGGTTGTGGATCAGAGGTTAGGGTTTCGAGTGCCGAAGATGTCGATTGCTGAGAAGTATTTGTTCGCAAAATCAAAGATGGAGGAATCAAAGAAATTCGCTGCACCTTCTACAACATATACATCTCAACCCTTTCAAACAAACACCGGTGGAATGGTTGACAATCGTGTACCAACAACTGCTGTTCGAATGTTTACGTCTGATAAATCAAACCACACATCAATGCCTTCTACAGTTTCCATGGCGTCTATGCCTCCCCATCTTGCTGCAGGATCTTCTGCACCATCGCAATATCAATCAACCAGCAATGAAGTGAGACCCCCCATTGTTTCTGGTGTAATGCCTAGCAGTCACATGGGGAGAAATCCATCTTCTGTAGCATTGCCTAGAGTTGAAAACCCACAGTTTAAAGTTACTGCAGGATTGAGTGGGGCTCCTTATGTCCTTCAGGTTCAAGCAAATTCATTGGCAAACCAATCATCAGTGAATGCTCCTTCATGGTCAATACAGACTCAACCTGTGTCATTAGGTAGAAATGTATCAGAAAACAAGGCGCCTGCTCATAACCCTGTCAAGGTTGAAGGAACTGCTGATGCAACCGTATCAAGGGCAGGTCCACAAGTAACAGCAGCTCAGAACATTAGGCCATTCATTACTCAAACGGGACCTGGAAATATGTCTACTATGCACCAGCCGTTGCAGGGTGGGAATATGGTTCGGCCTCCTTTGATACCTAGTCACTCTGATATTGCAAAGGTTGTCCAGAAGTTGTTAATACCAAAGCTTCCTGATCATCCCACATGGACTCCTCCGTCAAGAGATTATATGAGTAAGAATTTCACATGTCAGACGTGTGAGCTCACTGTCAGTGAGGTTGATTCTGTTCTTCTCTGTGATGCCTGTGAAAAGGGATTTCACTTGAAGTGCCTGCAGCCATCAGTCATTAGAGGAATTCATAATAGAGTTGATTGGCACTGCATGAAGTGCTTGGGTTTAAGCGGTGGAAAGCCTTTGCCTCCAAAATATGGCCGTGTGATGCGATCCTCAATTACATCACCAAATTTTCCCTCTAATTCAGCTGGTATTCAACCATCTTCAGAGAAGAAACCAGATAACTTAGATCCGAAGGTCAGCCCACAGATGTTAACAACAAATGGAAACTCTGTTCCAACTGGTTCCAGTACCAATCACAATACTGAGCCATCATTTGACTCAAATACCCCAGATACGAGAGATATACAAGGTTCCAACATTTCATCTAGCATTGAAACCATTGACGAGAAGCCTGATCCAAACATATGTGTGAAATCTGCAGCTTGTAGTGCTTCCACTGGCGTGCAGAGTGAAGGTTATGCTGAACAAATAGATTCTAAGGCTTTCGCCTGTAAAGATACTTCTGAATCTGAAACTCTTCCTAATATATCTGAGCTGGCTAAAAATGGAAATTTACAATCATCACCAGGTTCTCAAGTTGAGAATGCAGTGTCACAGGACAATGCTGAAATATCATCTGATAGACACGATAGCAGTAGCTTTATTATTAGTAATCAAAAGGAATCTCATGAAGGAGAAAGTACAACTTATGATATCAAGCGTGATGACCTAGATGCTGCACAACCGAATTCTGTTAGAGGTTCTGGAACTAATACCGAAGGTATTCAGCACTGTGCTTTATCTTCAGATAGCTCACATGCTGTAGAATGGATTGGTGATGTTGTACAACTTGTAGATGAGAAAAAGCATTACCAATCTTGCTGCATTGATGGAGTAACATATAGGCTGCAAGGTCATGCATTTTTCACGTCCAGCCATGGCAAACTAACACCTTCTAAACTTCAGTCCATGTGGGAAGATTCCAAAACTGGGGTGAAGTGGGTTAAGGTGACAAAGTGCTACTTTCCTGATGATTTGCCGGGGAATATTGGTCATCCTTGTATATCTGAAGTCAACGAGGTTTATGAATCTAATAGTGATAGAGTTGAAATGGCTAGTTCTATCCGAGGCCCTTGTGTAGTCCTTCCATATGATAAATTTAAACAAGAAAATGACAGAAGATGTCAATTTGGAGTTGAGGCAAGTGCTAGTGTACAACCCATTTTCCTTTGCAGGTGGTTTTATGATGAAATCAAAAAGTCATTTCAACCTGTTATCAGTTGA
Protein sequence

>MsaT029533.1

MEDVPVSEPTLDPPPEIENGEGEQQEERLSKKAKVEEKENSEAELKRVAEIVLVLSTMATMRSGKKPTDVEVELMREARTKLAVLCQGIAPKDIVGGEAIGSVIDDLGLNAKVVDQRLGFRVPKMSIAEKYLFAKSKMEESKKFAAPSTTYTSQPFQTNTGGMVDNRVPTTAVRMFTSDKSNHTSMPSTVSMASMPPHLAAGSSAPSQYQSTSNEVRPPIVSGVMPSSHMGRNPSSVALPRVENPQFKVTAGLSGAPYVLQVQANSLANQSSVNAPSWSIQTQPVSLGRNVSENKAPAHNPVKVEGTADATVSRAGPQVTAAQNIRPFITQTGPGNMSTMHQPLQGGNMVRPPLIPSHSDIAKVVQKLLIPKLPDHPTWTPPSRDYMSKNFTCQTCELTVSEVDSVLLCDACEKGFHLKCLQPSVIRGIHNRVDWHCMKCLGLSGGKPLPPKYGRVMRSSITSPNFPSNSAGIQPSSEKKPDNLDPKVSPQMLTTNGNSVPTGSSTNHNTEPSFDSNTPDTRDIQGSNISSSIETIDEKPDPNICVKSAACSASTGVQSEGYAEQIDSKAFACKDTSESETLPNISELAKNGNLQSSPGSQVENAVSQDNAEISSDRHDSSSFIISNQKESHEGESTTYDIKRDDLDAAQPNSVRGSGTNTEGIQHCALSSDSSHAVEWIGDVVQLVDEKKHYQSCCIDGVTYRLQGHAFFTSSHGKLTPSKLQSMWEDSKTGVKWVKVTKCYFPDDLPGNIGHPCISEVNEVYESNSDRVEMASSIRGPCVVLPYDKFKQENDRRCQFGVEASASVQPIFLCRWFYDEIKKSFQPVIS*