AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029591


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029591 MtrunA17_Chr5g0445491 98.990 396 2 1 2 395 108 503 0.0 796
MsaG029591 MtrunA17_Chr2g0294671 32.456 228 139 7 122 343 215 433 8.83e-27 111
MsaG029591 MtrunA17_Chr2g0294671 27.556 225 134 6 77 295 277 478 9.03e-18 85.1
MsaG029591 MtrunA17_Chr2g0294671 30.102 196 126 5 150 341 207 395 1.06e-17 84.7
MsaG029591 MtrunA17_Chr5g0428941 25.000 328 201 7 84 373 172 492 1.46e-26 111
MsaG029591 MtrunA17_Chr5g0428941 23.932 234 169 5 113 343 134 361 2.68e-11 65.1
MsaG029591 MtrunA17_Chr2g0311181 26.639 244 161 6 119 361 70 296 4.55e-26 107
MsaG029591 MtrunA17_Chr4g0043941 28.571 266 176 6 80 340 42 298 4.81e-25 104
MsaG029591 MtrunA17_Chr4g0043941 27.948 229 161 2 118 343 41 268 5.82e-20 90.1
MsaG029591 MtrunA17_Chr5g0417641 29.319 191 132 1 153 343 88 275 9.04e-22 94.7
MsaG029591 MtrunA17_Chr6g0448821 24.465 327 194 5 85 370 49 363 1.61e-21 95.1
MsaG029591 MtrunA17_Chr4g0034981 24.080 299 185 6 79 341 274 566 4.09e-20 92.4
MsaG029591 MtrunA17_Chr4g0034981 24.476 286 198 7 68 342 184 462 2.97e-13 71.2
MsaG029591 MtrunA17_Chr1g0212691 23.636 275 199 7 68 338 161 428 1.38e-18 87.4
MsaG029591 MtrunA17_Chr1g0212691 24.254 268 187 8 86 349 81 336 8.25e-16 79.0
MsaG029591 MtrunA17_Chr8g0391881 27.064 218 152 4 122 337 162 374 9.88e-18 84.0
MsaG029591 MtrunA17_Chr8g0391881 25.000 216 153 5 86 300 59 266 3.51e-14 73.6
MsaG029591 MtrunA17_Chr7g0250261 25.984 254 173 7 117 367 42 283 4.88e-17 80.9
MsaG029591 MtrunA17_Chr0c29g0494001 25.984 254 173 7 117 367 42 283 4.88e-17 80.9
MsaG029591 MtrunA17_Chr2g0304101 23.767 223 155 4 119 337 70 281 6.00e-17 80.9
MsaG029591 MtrunA17_Chr2g0304101 30.570 193 126 6 117 308 100 285 6.76e-13 68.9
MsaG029591 MtrunA17_Chr3g0094341 22.404 183 128 5 119 300 32 201 4.02e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029591 AT5G55580.1 68.250 400 118 2 4 394 96 495 0.0 558
MsaG029591 AT5G55580.2 73.950 357 89 1 38 394 37 389 0.0 554
MsaG029591 AT5G55580.3 64.548 299 97 2 4 293 96 394 2.27e-134 390
MsaG029591 AT2G21710.1 26.148 283 199 5 87 366 320 595 1.64e-26 112
MsaG029591 AT2G21710.1 23.975 317 221 8 68 373 191 498 5.69e-13 70.9
MsaG029591 AT4G02990.2 24.316 329 202 6 84 373 180 500 8.02e-25 106
MsaG029591 AT4G02990.1 24.316 329 202 6 84 373 180 500 8.02e-25 106
MsaG029591 AT4G38160.4 26.545 275 188 6 72 340 34 300 1.10e-23 100
MsaG029591 AT4G38160.4 29.741 232 155 6 118 343 41 270 1.93e-21 94.7
MsaG029591 AT4G38160.1 26.545 275 188 6 72 340 34 300 1.10e-23 100
MsaG029591 AT4G38160.1 29.741 232 155 6 118 343 41 270 1.93e-21 94.7
MsaG029591 AT4G38160.2 26.545 275 188 6 72 340 34 300 1.31e-23 101
MsaG029591 AT4G38160.2 29.741 232 155 6 118 343 41 270 2.23e-21 95.1
MsaG029591 AT4G38160.3 26.545 275 188 6 72 340 34 300 2.13e-23 100
MsaG029591 AT4G38160.3 29.741 232 155 6 118 343 41 270 3.20e-21 94.7
MsaG029591 AT1G78930.1 27.650 217 153 3 124 340 342 554 4.82e-23 101
MsaG029591 AT1G78930.1 28.485 165 110 3 112 273 397 556 1.78e-14 75.5
MsaG029591 AT2G03050.1 26.339 224 155 5 119 340 33 248 2.04e-21 93.6
MsaG029591 AT2G03050.2 26.339 224 155 5 119 340 34 249 2.05e-21 93.6
MsaG029591 AT4G14605.2 26.484 219 154 5 122 340 236 447 8.92e-21 94.4
MsaG029591 AT4G14605.2 26.222 225 137 6 77 295 262 463 1.89e-14 75.1
MsaG029591 AT4G14605.1 26.484 219 154 5 122 340 236 447 8.92e-21 94.4
MsaG029591 AT4G14605.1 26.222 225 137 6 77 295 262 463 1.89e-14 75.1
MsaG029591 AT2G44020.1 26.421 299 174 7 84 342 145 437 1.46e-20 94.0
MsaG029591 AT2G44020.1 26.786 224 148 7 123 343 88 298 2.83e-12 68.6
MsaG029591 AT2G34620.1 26.606 218 146 4 152 365 85 292 1.56e-20 91.7
MsaG029591 AT2G21710.2 24.481 241 175 4 87 324 320 556 2.64e-20 93.2
MsaG029591 AT2G21710.2 23.975 317 221 8 68 373 191 498 6.49e-13 70.5
MsaG029591 AT2G21710.3 24.268 239 174 4 87 322 320 554 1.20e-19 91.3
MsaG029591 AT2G21710.3 23.975 317 221 8 68 373 191 498 5.93e-13 70.9
MsaG029591 AT2G36000.2 27.200 250 158 8 119 362 74 305 1.83e-19 89.0
MsaG029591 AT2G36000.1 27.200 250 158 8 119 362 74 305 2.43e-19 88.6
MsaG029591 AT3G18870.1 25.197 254 132 8 122 367 69 272 4.23e-17 81.3

Find 74 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAAGCACCTTTGGAATTA+AGG 0.140140 5:+79139248 MsaT029591.1:CDS
AGCAATAATTTGCCCTATTT+TGG 0.169836 5:-79139487 None:intergenic
GACTCACGTTGCTTTCTTAA+TGG 0.188627 5:+79139446 MsaT029591.1:CDS
CATCTTCACAATGCTCTCTT+TGG 0.219102 5:-79139703 None:intergenic
TGACTTCAATCTCTTGTCTT+TGG 0.225373 5:-79138378 None:intergenic
CGTTGCTTTCTTAATGGGCT+TGG 0.237557 5:+79139452 MsaT029591.1:CDS
GGATGCTATTCTCTGACTTT+TGG 0.242258 5:-79138749 None:intergenic
ACATGTGCCTCTCATAAATT+TGG 0.244120 5:-79139284 None:intergenic
CTCCTTAGGAAATTTATTCT+TGG 0.307382 5:-79139777 None:intergenic
TCCAACGTATTGACATTTCA+TGG 0.312784 5:+79139640 MsaT029591.1:CDS
ATCTTCACAATGCTCTCTTT+GGG 0.327138 5:-79139702 None:intergenic
GCTAAGGTACATCGGGTATT+TGG 0.329609 5:-79140103 None:intergenic
CCAAGAGGAAATGGTCCTTT+TGG 0.331786 5:-79140186 None:intergenic
CTTGATTACTTAAGCACCTT+TGG 0.335021 5:+79139240 MsaT029591.1:CDS
TCGTCCTCGTCATAAGTTCT+TGG 0.344950 5:+79140146 MsaT029591.1:CDS
ACTCACGTTGCTTTCTTAAT+GGG 0.345489 5:+79139447 MsaT029591.1:CDS
GATGTACCTTAGCTTGTCTT+TGG 0.348758 5:+79140113 MsaT029591.1:CDS
GGCGTACCTAGTACCAAAAT+AGG 0.359195 5:+79139474 MsaT029591.1:CDS
TAGATTACTTGATGAGTCTT+GGG 0.360371 5:+79139346 MsaT029591.1:CDS
GTTGCTTTCTTAATGGGCTT+GGG 0.365837 5:+79139453 MsaT029591.1:CDS
GTTGTCTGAAGAGATTCTTT+TGG 0.368169 5:+79139197 MsaT029591.1:CDS
TTGAAGTCAAAGACAGTTGC+AGG 0.386479 5:+79138392 MsaT029591.1:CDS
GAATTTGTGGTTGCCTCAAA+AGG 0.421558 5:-79139395 None:intergenic
ATAGATTACTTGATGAGTCT+TGG 0.425881 5:+79139345 MsaT029591.1:CDS
CTGCATTACAGTATTGATGA+TGG 0.426933 5:+79139747 MsaT029591.1:CDS
ACCGTGTATTCTAGAATTTG+TGG 0.436537 5:-79139408 None:intergenic
AGATATTTAATTGAGGAAGT+TGG 0.438021 5:+79139564 MsaT029591.1:CDS
GCAGGTGCTATCTCTGTCCT+TGG 0.454121 5:+79140014 MsaT029591.1:intron
CAAAGAGAGCATTGTGAAGA+TGG 0.456446 5:+79139704 MsaT029591.1:CDS
ACTAGAGATCCAAGAGGAAA+TGG 0.462060 5:-79140195 None:intergenic
TAGACAACAAGAAAAGGAAA+AGG 0.464265 5:+79138687 MsaT029591.1:CDS
CCAAAAGGACCATTTCCTCT+TGG 0.467379 5:+79140186 MsaT029591.1:CDS
TGACAAAAGGATTCATCAGT+TGG 0.470816 5:-79140219 None:intergenic
TTCGATGGAGAAAAGATATC+CGG 0.473213 5:+79139173 MsaT029591.1:CDS
TATGACGAGGACGAATTCTC+TGG 0.474277 5:-79140137 None:intergenic
TCCAGCCCATCGTTGACAAA+AGG 0.483266 5:-79140232 None:intergenic
AATTGAGGAAGTTGGCATCA+AGG 0.486070 5:+79139572 MsaT029591.1:CDS
TCAATTAAATATCTTACTGT+TGG 0.487640 5:-79139555 None:intergenic
GATGTTTCTTAACAAAGAGT+TGG 0.488384 5:+79139674 MsaT029591.1:CDS
TACAGATAGACAACAAGAAA+AGG 0.490910 5:+79138681 MsaT029591.1:intron
TTCCAAGAATAAATTTCCTA+AGG 0.495242 5:+79139775 MsaT029591.1:CDS
AAAGCCGAAGTATTTGTACT+TGG 0.508230 5:+79140047 MsaT029591.1:CDS
GCGTACCTAGTACCAAAATA+GGG 0.510052 5:+79139475 MsaT029591.1:CDS
AACAGTAAGATATTTAATTG+AGG 0.516231 5:+79139557 MsaT029591.1:CDS
TTGTGATTCCTTAATTCCAA+AGG 0.518718 5:-79139256 None:intergenic
TCTTTGGCATTAAGATCAGT+AGG 0.525087 5:-79138362 None:intergenic
AATAAATGTATGTTCTGCAC+AGG 0.526298 5:+79139317 MsaT029591.1:CDS
CGGCTTTAGATTAGCTTCCA+AGG 0.526666 5:-79140031 None:intergenic
ATAAATTTCCTAAGGAGCAT+AGG 0.526985 5:+79139783 MsaT029591.1:CDS
TTTACCAAGTACAAATACTT+CGG 0.532246 5:-79140051 None:intergenic
AAGACAAGCTAAGGTACATC+GGG 0.535136 5:-79140110 None:intergenic
GTAGTTTCGTAGAAATTCGA+TGG 0.537172 5:+79139158 MsaT029591.1:intron
GTACATCGGGTATTTGGTCA+AGG 0.541793 5:-79140097 None:intergenic
TCCCTAAAGAAAGCTCCAAA+AGG 0.551170 5:+79140171 MsaT029591.1:CDS
TCTGGTCCAAAGACAAGCTA+AGG 0.551617 5:-79140119 None:intergenic
AGACAACAAGAAAAGGAAAA+GGG 0.551962 5:+79138688 MsaT029591.1:CDS
ATACGTTGGACAAGAATTTG+AGG 0.570242 5:-79139627 None:intergenic
ATGTATGTTCTGCACAGGAA+AGG 0.582720 5:+79139322 MsaT029591.1:CDS
AGATTACTTGATGAGTCTTG+GGG 0.589186 5:+79139347 MsaT029591.1:CDS
AAAGACAAGCTAAGGTACAT+CGG 0.593674 5:-79140111 None:intergenic
AGAATCTCTTCAGACAACTC+CGG 0.598051 5:-79139192 None:intergenic
GTGCTTAAGTAATCAAGAAG+TGG 0.598694 5:-79139234 None:intergenic
ATACTGTAATGCAGAAGCTG+AGG 0.609678 5:-79139738 None:intergenic
ACAAGAAAATAACAGTGCAA+AGG 0.615006 5:+79138774 MsaT029591.1:CDS
TACTGTAATGCAGAAGCTGA+GGG 0.620289 5:-79139737 None:intergenic
TCCATGAAATGTCAATACGT+TGG 0.626915 5:-79139641 None:intergenic
GGTAAATGAGCTTCACAATG+AGG 0.633064 5:+79140068 MsaT029591.1:CDS
GGTGTGAGATCTCTTAACAA+TGG 0.634820 5:+79138709 MsaT029591.1:CDS
AATTCATCCAAATTTATGAG+AGG 0.636805 5:+79139277 MsaT029591.1:CDS
AATCTTGACTAGCCTTACAC+AGG 0.642412 5:+79139830 MsaT029591.1:CDS
ACCACAAATTCTAGAATACA+CGG 0.657650 5:+79139407 MsaT029591.1:CDS
GTTGGAACTAGAGATCCAAG+AGG 0.745944 5:-79140201 None:intergenic
GAAACCAAGAACTTATGACG+AGG 0.752928 5:-79140150 None:intergenic
ACAAATTCTAGAATACACGG+TGG 0.791192 5:+79139410 MsaT029591.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 79138261 79140335 79138261 ID=MsaG029591
Chr5 mRNA 79138261 79140335 79138261 ID=MsaT029591.1;Parent=MsaG029591
Chr5 exon 79138261 79138263 79138261 ID=MsaT029591.1.exon1;Parent=MsaT029591.1
Chr5 CDS 79138261 79138263 79138261 ID=cds.MsaT029591.1;Parent=MsaT029591.1
Chr5 exon 79138347 79138413 79138347 ID=MsaT029591.1.exon2;Parent=MsaT029591.1
Chr5 CDS 79138347 79138413 79138347 ID=cds.MsaT029591.1;Parent=MsaT029591.1
Chr5 exon 79138686 79138795 79138686 ID=MsaT029591.1.exon3;Parent=MsaT029591.1
Chr5 CDS 79138686 79138795 79138686 ID=cds.MsaT029591.1;Parent=MsaT029591.1
Chr5 exon 79139162 79139851 79139162 ID=MsaT029591.1.exon4;Parent=MsaT029591.1
Chr5 CDS 79139162 79139851 79139162 ID=cds.MsaT029591.1;Parent=MsaT029591.1
Chr5 exon 79140018 79140335 79140018 ID=MsaT029591.1.exon5;Parent=MsaT029591.1
Chr5 CDS 79140018 79140335 79140018 ID=cds.MsaT029591.1;Parent=MsaT029591.1
Gene Sequence

>MsaT029591.1

ATGGACGGATTTGCTGAGCCTACTGATCTTAATGCCAAAGACAAGAGATTGAAGTCAAAGACAGTTGCAGATAGACAACAAGAAAAGGAAAAGGGTGTGAGATCTCTTAACAATGGACAGAGCAAAAGATTGCCCAAAAGTCAGAGAATAGCATCCTTACAAGAAAATAACAGTGCAAAGTTTCGTAGAAATTCGATGGAGAAAAGATATCCGGAGTTGTCTGAAGAGATTCTTTTGGATGAGAAATGGTTACCACTTCTTGATTACTTAAGCACCTTTGGAATTAAGGAATCACAATTCATCCAAATTTATGAGAGGCACATGTCGTCATTTCAAATAAATGTATGTTCTGCACAGGAAAGGATAGATTACTTGATGAGTCTTGGGGTTAAACATAAAGATATAAGAAGAATCCTTTTGAGGCAACCACAAATTCTAGAATACACGGTGGAGAACAATTTGAAGACTCACGTTGCTTTCTTAATGGGCTTGGGCGTACCTAGTACCAAAATAGGGCAAATTATTGCTTCTACTCCGTCCCTTTTTTCTTACAGTGTTGAGAAATCGTTAAAACCAACAGTAAGATATTTAATTGAGGAAGTTGGCATCAAGGAAAAAGATTTGGGTAAAGTCATTCAACTGAGTCCTCAAATTCTTGTCCAACGTATTGACATTTCATGGAATACTCGATTGATGTTTCTTAACAAAGAGTTGGATGCACCCAAAGAGAGCATTGTGAAGATGGTGACGAAACACCCTCAGCTTCTGCATTACAGTATTGATGATGGATTGCTTCCAAGAATAAATTTCCTAAGGAGCATAGGAATGAAAAATGCGGACATCTTGAAAATCTTGACTAGCCTTACACAGGTGCTATCTCTGTCCTTGGAAGCTAATCTAAAGCCGAAGTATTTGTACTTGGTAAATGAGCTTCACAATGAGGTGCAAACCTTGACCAAATACCCGATGTACCTTAGCTTGTCTTTGGACCAGAGAATTCGTCCTCGTCATAAGTTCTTGGTTTCCCTAAAGAAAGCTCCAAAAGGACCATTTCCTCTTGGATCTCTAGTTCCAACTGATGAATCCTTTTGTCAACGATGGGCTGGAACTACTCTTGATGAATATGCTGCATTTCGACAGAGATTATTGCTCAAAAAACTTGCTGAGAAATATGACAGAAAAATGTGA

Protein sequence

>MsaT029591.1

MDGFAEPTDLNAKDKRLKSKTVADRQQEKEKGVRSLNNGQSKRLPKSQRIASLQENNSAKFRRNSMEKRYPELSEEILLDEKWLPLLDYLSTFGIKESQFIQIYERHMSSFQINVCSAQERIDYLMSLGVKHKDIRRILLRQPQILEYTVENNLKTHVAFLMGLGVPSTKIGQIIASTPSLFSYSVEKSLKPTVRYLIEEVGIKEKDLGKVIQLSPQILVQRIDISWNTRLMFLNKELDAPKESIVKMVTKHPQLLHYSIDDGLLPRINFLRSIGMKNADILKILTSLTQVLSLSLEANLKPKYLYLVNELHNEVQTLTKYPMYLSLSLDQRIRPRHKFLVSLKKAPKGPFPLGSLVPTDESFCQRWAGTTLDEYAAFRQRLLLKKLAEKYDRKM*