AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029702


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029702 MtrunA17_Chr5g0447111 93.636 330 19 1 1 328 1 330 0.0 637
MsaG029702 MtrunA17_Chr3g0103031 64.438 329 114 3 1 327 1 328 9.96e-152 429
MsaG029702 MtrunA17_Chr1g0159831 45.872 327 170 4 7 327 5 330 1.12e-101 302
MsaG029702 MtrunA17_Chr3g0124921 47.826 322 163 5 5 323 2 321 3.47e-101 300
MsaG029702 MtrunA17_Chr5g0447191 54.913 173 77 1 152 323 15 187 3.05e-60 191
MsaG029702 MtrunA17_Chr4g0029841 32.143 224 128 5 98 321 77 276 7.42e-27 107
MsaG029702 MtrunA17_Chr3g0114411 34.337 166 101 4 133 292 70 233 3.84e-13 68.6
MsaG029702 MtrunA17_Chr7g0259851 28.462 130 91 2 163 292 526 653 1.82e-11 65.1
MsaG029702 MtrunA17_Chr3g0125321 28.289 152 103 3 174 323 94 241 2.16e-11 63.2
MsaG029702 MtrunA17_Chr5g0394261 27.660 141 99 3 163 302 536 674 3.17e-11 64.3
MsaG029702 MtrunA17_Chr1g0185861 26.347 167 111 3 137 292 185 350 3.32e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029702 AT1G55760.1 56.098 328 142 2 1 327 1 327 1.37e-134 385
MsaG029702 AT1G21780.2 49.068 322 161 3 7 327 5 324 1.04e-111 327
MsaG029702 AT1G21780.1 49.068 322 161 3 7 327 5 324 1.04e-111 327
MsaG029702 AT1G01640.1 30.811 185 124 4 145 327 6 188 9.55e-16 75.1
MsaG029702 AT1G01640.2 30.178 169 114 4 161 327 1 167 3.93e-14 70.1
MsaG029702 AT3G56230.1 32.847 137 89 3 159 292 107 243 6.02e-14 71.6
MsaG029702 AT2G30600.4 30.282 142 94 3 139 275 327 468 1.74e-12 68.6
MsaG029702 AT2G30600.1 30.282 142 94 3 139 275 327 468 1.74e-12 68.6
MsaG029702 AT2G30600.3 30.282 142 94 3 139 275 327 468 1.74e-12 68.6
MsaG029702 AT2G30600.2 30.282 142 94 3 139 275 327 468 1.74e-12 68.6
MsaG029702 AT2G30600.6 30.282 142 94 3 139 275 327 468 2.17e-12 68.6
MsaG029702 AT2G39760.2 28.049 164 104 3 139 291 172 332 3.23e-12 67.0
MsaG029702 AT2G39760.4 28.049 164 104 3 139 291 172 332 4.02e-12 66.6
MsaG029702 AT2G39760.3 28.049 164 104 3 139 291 172 332 4.02e-12 66.6
MsaG029702 AT1G05690.1 26.012 173 114 6 137 301 29 195 7.20e-12 66.2
MsaG029702 AT2G39760.1 28.049 164 104 3 139 291 172 332 1.01e-11 65.9
MsaG029702 AT4G08455.1 37.500 96 55 2 175 268 107 199 2.79e-11 63.5
MsaG029702 AT5G63160.2 28.824 170 114 4 161 325 23 190 3.02e-11 63.9
MsaG029702 AT5G63160.1 28.824 170 114 4 161 325 23 190 3.41e-11 63.9
MsaG029702 AT2G30600.5 29.452 146 93 4 139 279 327 467 4.99e-11 64.3

Find 46 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAATTGACATTGAGTTTC+TGG 0.099574 5:+80338368 MsaT029702.1:CDS
GTGCAATATCTTGTGAAATT+TGG 0.214576 5:+80339146 MsaT029702.1:CDS
ATTGATACAAAGATGCAATT+TGG 0.251499 5:-80339100 None:intergenic
TGACATGTCGGTGATGTTTA+TGG 0.257723 5:-80338898 None:intergenic
GTGGGGAAGCTTGCTCTATT+TGG 0.265550 5:+80338673 MsaT029702.1:CDS
CCATTGAACTAGACGATTCA+TGG 0.288711 5:-80337847 None:intergenic
CAATTGCATCTTTCATAGTT+AGG 0.300214 5:+80338101 MsaT029702.1:CDS
GATAGCTTTGTGTGGGAGAT+TGG 0.337706 5:+80338324 MsaT029702.1:CDS
AGGATTGTGTGTGATTGTTT+TGG 0.337739 5:-80338077 None:intergenic
AGAATTTCTTATACATAGAT+TGG 0.343658 5:+80338974 MsaT029702.1:CDS
ACTTGTCAGCCGCATGTAAT+AGG 0.362904 5:-80338998 None:intergenic
TCAAAGAAATTCTTGATGCT+TGG 0.368629 5:+80339243 MsaT029702.1:CDS
AGATAAACTTCTTAGCAATA+AGG 0.369571 5:+80338302 MsaT029702.1:CDS
AGCAAAATCTTGACTCAATC+AGG 0.374788 5:+80338145 MsaT029702.1:CDS
TCAAAATAGCCATGTGGAAC+TGG 0.403978 5:+80337916 MsaT029702.1:CDS
CGTTGATTGTTATGTCTGTA+TGG 0.409784 5:-80338770 None:intergenic
GTTAGGATTCTTAGTTATGC+AGG 0.419838 5:+80338118 MsaT029702.1:CDS
AAAGAAAATTTATAAACGTC+TGG 0.434882 5:-80338932 None:intergenic
TTCTCCTATCATTCGACCTA+GGG 0.439636 5:-80338742 None:intergenic
CTAACTATGAAAGATGCAAT+TGG 0.440177 5:-80338100 None:intergenic
ATGAGCTCTTATGCTTCCTT+CGG 0.452327 5:-80338799 None:intergenic
CCATGAATCGTCTAGTTCAA+TGG 0.453483 5:+80337847 MsaT029702.1:CDS
GCTTGCTCTATTTGGCCTGA+TGG 0.459699 5:+80338681 MsaT029702.1:CDS
TCATTCGACCTAGGGATTCA+AGG 0.469041 5:-80338734 None:intergenic
GAATCCCTAGGTCGAATGAT+AGG 0.475257 5:+80338738 MsaT029702.1:CDS
GGCAAAATATATGATATTCA+AGG 0.488841 5:+80339167 MsaT029702.1:CDS
CTCGGCTGCATAAATCCATC+AGG 0.508065 5:-80338696 None:intergenic
TTTCTCCTATCATTCGACCT+AGG 0.524682 5:-80338743 None:intergenic
GAGATTGGAGTTCCATTACC+TGG 0.545273 5:+80338339 MsaT029702.1:CDS
CATTCGACCTAGGGATTCAA+GGG 0.553806 5:-80338733 None:intergenic
ACTATGAAAGATGCAATTGG+AGG 0.556805 5:-80338097 None:intergenic
CAATAAGGATAGCTTTGTGT+GGG 0.566302 5:+80338317 MsaT029702.1:CDS
TTTGAAAATTACATGTCCAA+AGG 0.574384 5:+80338392 MsaT029702.1:CDS
GCAGCGGCCCTTGAATCCCT+AGG 0.588324 5:+80338726 MsaT029702.1:CDS
GATATGAAGGCATTTATCAG+TGG 0.615660 5:+80338012 MsaT029702.1:intron
GCAATAAGGATAGCTTTGTG+TGG 0.617349 5:+80338316 MsaT029702.1:CDS
ACAATCAACGTCAATTCCGA+AGG 0.618813 5:+80338783 MsaT029702.1:CDS
TTGACTTTACCAGTTCCACA+TGG 0.646739 5:-80337925 None:intergenic
TTATCAGTGGAAAAGAACTG+TGG 0.666641 5:+80338025 MsaT029702.1:CDS
GAAAGATTGAATTGACATGT+CGG 0.685189 5:-80338910 None:intergenic
GTCAAACACAGGTGATTGTG+AGG 0.685218 5:-80338835 None:intergenic
TAGATTGGACCTATTACATG+CGG 0.685610 5:+80338989 MsaT029702.1:CDS
TGATATCTATGAGTTAAGAG+AGG 0.687061 5:+80339022 MsaT029702.1:CDS
GAAAACATGCTGTCAAACAC+AGG 0.701282 5:-80338846 None:intergenic
ATCCATTCAAAATAGCCATG+TGG 0.702367 5:+80337910 MsaT029702.1:CDS
GCAGCCGAGACAAAATGCAG+CGG 0.710020 5:+80338710 MsaT029702.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 80337819 80339274 80337819 ID=MsaG029702
Chr5 mRNA 80337819 80339274 80337819 ID=MsaT029702.1;Parent=MsaG029702
Chr5 exon 80337819 80337937 80337819 ID=MsaT029702.1.exon1;Parent=MsaT029702.1
Chr5 CDS 80337819 80337937 80337819 ID=cds.MsaT029702.1;Parent=MsaT029702.1
Chr5 exon 80338021 80338166 80338021 ID=MsaT029702.1.exon2;Parent=MsaT029702.1
Chr5 CDS 80338021 80338166 80338021 ID=cds.MsaT029702.1;Parent=MsaT029702.1
Chr5 exon 80338295 80338413 80338295 ID=MsaT029702.1.exon3;Parent=MsaT029702.1
Chr5 CDS 80338295 80338413 80338295 ID=cds.MsaT029702.1;Parent=MsaT029702.1
Chr5 exon 80338672 80339274 80338672 ID=MsaT029702.1.exon4;Parent=MsaT029702.1
Chr5 CDS 80338672 80339274 80338672 ID=cds.MsaT029702.1;Parent=MsaT029702.1
Gene Sequence

>MsaT029702.1

ATGAATGATTTTGCATACAAAGTTGAAACCATGAATCGTCTAGTTCAATGGAAAATTCACAATTTATCAACATGCACTTATATCAAAGCTGATCCATTCAAAATAGCCATGTGGAACTGGCATTTATCAGTGGAAAAGAACTGTGGTTTACGTATTGAATTATATCCAGAAATGTCCAAAACAATCACACACAATCCTCCAATTGCATCTTTCATAGTTAGGATTCTTAGTTATGCAGGAAATAGCAAAATCTTGACTCAATCAGAAATAAAAGATAAACTTCTTAGCAATAAGGATAGCTTTGTGTGGGAGATTGGAGTTCCATTACCTGGAAAATTCATAATTGACATTGAGTTTCTGGATTTGAAAATTACATGTCCAAAGGGTGGGGAAGCTTGCTCTATTTGGCCTGATGGATTTATGCAGCCGAGACAAAATGCAGCGGCCCTTGAATCCCTAGGTCGAATGATAGGAGAAAGTATCCATACAGACATAACAATCAACGTCAATTCCGAAGGAAGCATAAGAGCTCATCGCGCTGTTCTTGCCTCACAATCACCTGTGTTTGACAGCATGTTTTCACACAATGTTAAGGAGACTGATTCGTCTACCATAAACATCACCGACATGTCAATTCAATCTTTCCAGACGTTTATAAATTTTCTTTATGGCAATGTCAATGATGAAGAATTTCTTATACATAGATTGGACCTATTACATGCGGCTGACAAGTATGATATCTATGAGTTAAGAGAGGCATGTGAAAAGAGTCTTCAAGAAGATATTGATCCAAAAAATGTACTTGAGAGACTCCAAATTGCATCTTTGTATCAATTAACAACGTTGAAGTTTAGTTGTGTGCAATATCTTGTGAAATTTGGCAAAATATATGATATTCAAGGTGATTTAAGTTCATTCTTGCAAACTGCTGATAGAGATTTGATATGTGAAGTCTTCAAAGAAATTCTTGATGCTTGGAAAAAGTGA

Protein sequence

>MsaT029702.1

MNDFAYKVETMNRLVQWKIHNLSTCTYIKADPFKIAMWNWHLSVEKNCGLRIELYPEMSKTITHNPPIASFIVRILSYAGNSKILTQSEIKDKLLSNKDSFVWEIGVPLPGKFIIDIEFLDLKITCPKGGEACSIWPDGFMQPRQNAAALESLGRMIGESIHTDITINVNSEGSIRAHRAVLASQSPVFDSMFSHNVKETDSSTINITDMSIQSFQTFINFLYGNVNDEEFLIHRLDLLHAADKYDIYELREACEKSLQEDIDPKNVLERLQIASLYQLTTLKFSCVQYLVKFGKIYDIQGDLSSFLQTADRDLICEVFKEILDAWKK*