AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029709


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029709 MtrunA17_Chr5g0447191 95.092 163 8 0 160 322 21 183 1.69e-110 319
MsaG029709 MtrunA17_Chr5g0447111 43.385 325 149 10 20 322 10 321 3.64e-75 234
MsaG029709 MtrunA17_Chr3g0103031 43.807 331 150 9 20 327 8 325 3.44e-74 232
MsaG029709 MtrunA17_Chr3g0124921 34.513 339 182 11 16 329 1 324 2.50e-51 172
MsaG029709 MtrunA17_Chr1g0159831 32.326 331 182 10 20 322 6 322 5.58e-48 164
MsaG029709 MtrunA17_Chr4g0029841 35.047 214 117 3 116 322 76 274 4.77e-31 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029709 AT1G55760.1 41.846 325 154 9 20 322 8 319 3.09e-70 222
MsaG029709 AT1G21780.2 36.615 325 170 11 20 322 6 316 1.67e-55 184
MsaG029709 AT1G21780.1 36.615 325 170 11 20 322 6 316 1.67e-55 184

Find 56 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCAATATCTAGTGGAATT+TGG 0.233852 5:-80440627 MsaT029709.1:CDS
AAAACCAATGAACCATTTCT+TGG 0.257328 5:-80444106 None:intergenic
TGATTAAATGCACGTACATT+TGG 0.280709 5:+80441230 None:intergenic
TTGGTAAAATACATGAAATC+TGG 0.300649 5:-80440608 MsaT029709.1:CDS
GGTTTGCAGTCTTGAGATCT+AGG 0.312817 5:+80441252 None:intergenic
TCAAGATATCTAAATGGAAT+TGG 0.352470 5:-80442198 MsaT029709.1:intron
GCGAAACTGATGCATTTAAC+TGG 0.358814 5:-80441328 MsaT029709.1:CDS
AAGCTTTCAAGATATCTAAA+TGG 0.359355 5:-80442204 MsaT029709.1:CDS
GATTAAATGCACGTACATTT+GGG 0.365879 5:+80441231 None:intergenic
TATGTTTGCACAAGATTCTA+AGG 0.372257 5:-80440913 MsaT029709.1:CDS
GCATATAATTTGATAGTAAC+CGG 0.381945 5:+80441915 None:intergenic
TTTAGATATCTTGAAAGCTT+CGG 0.402228 5:+80442207 None:intergenic
GAATCATAGTTTCGAGCTAT+TGG 0.408610 5:-80440790 MsaT029709.1:CDS
ATGAATGCTTGACAAACTTC+AGG 0.409567 5:+80440852 None:intergenic
TCATCCAAGAAATGGTTCAT+TGG 0.410202 5:+80444102 None:intergenic
ATCTAGCTGTTCTTGCTGCC+CGG 0.416939 5:-80440953 MsaT029709.1:CDS
CGGAATTTGAAAGATGCAAT+TGG 0.422404 5:+80441870 None:intergenic
GACCATCATCATCCAAGAAA+TGG 0.432953 5:+80444094 None:intergenic
CCGTGGGTTACGTCGCTCAA+TGG 0.441645 5:-80442282 MsaT029709.1:CDS
GATAGTAACCGGACTGGAAA+GGG 0.453381 5:+80441926 None:intergenic
TGCAATTGGTGGATTATTTG+TGG 0.455591 5:+80441884 None:intergenic
TTCCGATAGAGTCAAGAATA+CGG 0.466943 5:+80441850 None:intergenic
TTCCGTATTCTTGACTCTAT+CGG 0.469882 5:-80441852 MsaT029709.1:CDS
GAGGGATTCCCTTTCCAGTC+CGG 0.470003 5:-80441934 MsaT029709.1:CDS
TGGTAAAATACATGAAATCT+GGG 0.484453 5:-80440607 MsaT029709.1:CDS
TTGGTTGCTGCTGAGAAATA+CGG 0.484832 5:-80440771 MsaT029709.1:CDS
TGGAATAACCTTCGCAGAGA+CGG 0.491897 5:-80441823 MsaT029709.1:CDS
GGAATAACCTTCGCAGAGAC+GGG 0.508589 5:-80441822 MsaT029709.1:intron
AATTTGAAAGATGCAATTGG+TGG 0.524532 5:+80441873 None:intergenic
AACCATTTCTTGGATGATGA+TGG 0.525067 5:-80444096 MsaT029709.1:CDS
ACACAGGATGTTGTAACATC+AGG 0.530431 5:+80441287 None:intergenic
GTTAAATGCATCAGTTTCGC+TGG 0.535589 5:+80441331 None:intergenic
TATATTCAAACATAGGTGAC+CGG 0.536701 5:+80440934 None:intergenic
TGATAGTAACCGGACTGGAA+AGG 0.543693 5:+80441925 None:intergenic
GATACTTTCTCTTTCGAACA+AGG 0.552217 5:+80441028 None:intergenic
AGCGCAACTAATCTTCAAGT+AGG 0.555426 5:+80440647 None:intergenic
TCTTGAAAGCTTCGGATGTG+TGG 0.556978 5:+80442215 None:intergenic
TCCATTCAAAGTGGAATCCG+TGG 0.560598 5:-80442299 MsaT029709.1:intron
GCAAACATATATTCAAACAT+AGG 0.563128 5:+80440927 None:intergenic
CGAAACTGATGCATTTAACT+GGG 0.567854 5:-80441327 MsaT029709.1:CDS
TAATTTGATAGTAACCGGAC+TGG 0.592751 5:+80441920 None:intergenic
GCGCAACTAATCTTCAAGTA+GGG 0.602481 5:+80440648 None:intergenic
TAGGAATTCAACTTCAACAC+AGG 0.603549 5:+80441271 None:intergenic
CCATTGAGCGACGTAACCCA+CGG 0.604930 5:+80442282 None:intergenic
CGGCGTTAATGATTTGAGAG+AGG 0.605267 5:-80440751 MsaT029709.1:CDS
ATACTTTCTCTTTCGAACAA+GGG 0.607778 5:+80441029 None:intergenic
GGATGTGTGGATAAAACTGT+GGG 0.608090 5:+80442228 None:intergenic
TAGTTGCGCTCAATATCTAG+TGG 0.608180 5:-80440634 MsaT029709.1:CDS
CCATTCAAAGTGGAATCCGT+GGG 0.610952 5:-80442298 MsaT029709.1:intron
ATTGATAGAAATATGCAGCA+TGG 0.618155 5:+80440673 None:intergenic
AAAGGCACATATCATTCTCG+AGG 0.636899 5:-80441953 MsaT029709.1:intron
CGGATGTGTGGATAAAACTG+TGG 0.637469 5:+80442227 None:intergenic
ATATTCAAACATAGGTGACC+GGG 0.654376 5:+80440935 None:intergenic
AAGGCACATATCATTCTCGA+GGG 0.667706 5:-80441952 MsaT029709.1:CDS
GCTTGATTGTAGCGTCTGTG+TGG 0.704769 5:+80441006 None:intergenic
GATGTGTGGATAAAACTGTG+GGG 0.837656 5:+80442229 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 80440515 80444121 80440515 ID=MsaG029709
Chr5 mRNA 80440515 80444121 80440515 ID=MsaT029709.1;Parent=MsaG029709
Chr5 exon 80440515 80441065 80440515 ID=MsaT029709.1.exon5;Parent=MsaT029709.1
Chr5 CDS 80440515 80441065 80440515 ID=cds.MsaT029709.1;Parent=MsaT029709.1
Chr5 exon 80441243 80441374 80441243 ID=MsaT029709.1.exon4;Parent=MsaT029709.1
Chr5 CDS 80441243 80441374 80441243 ID=cds.MsaT029709.1;Parent=MsaT029709.1
Chr5 exon 80441823 80441971 80441823 ID=MsaT029709.1.exon3;Parent=MsaT029709.1
Chr5 CDS 80441823 80441971 80441823 ID=cds.MsaT029709.1;Parent=MsaT029709.1
Chr5 exon 80442199 80442310 80442199 ID=MsaT029709.1.exon2;Parent=MsaT029709.1
Chr5 CDS 80442199 80442310 80442199 ID=cds.MsaT029709.1;Parent=MsaT029709.1
Chr5 exon 80444064 80444121 80444064 ID=MsaT029709.1.exon1;Parent=MsaT029709.1
Chr5 CDS 80444064 80444121 80444064 ID=cds.MsaT029709.1;Parent=MsaT029709.1
Gene Sequence

>MsaT029709.1

ATGAACCATTTCTTGGATGATGATGGTCCTCTGTCCAAAAATGAGGTGGTAGACCTAGTGGAATCCGTGGGTTACGTCGCTCAATGGAGACTCAGCTTTAAGAGCATGAATATGATTCCCCACAGTTTTATCCACACATCCGAAGCTTTCAAGATATCTAAATGGAATTGGCACATATCATTCTCGAGGGATTCCCTTTCCAGTCCGGTTACTATCAAATTATATGCAGAAGAGTCCACAAATAATCCACCAATTGCATCTTTCAAATTCCGTATTCTTGACTCTATCGGAAAAAATGGAATAACCTTCGCAGAGACGGAAGAAAAAGACAAGCTTTTCACCAGCGAAACTGATGCATTTAACTGGGAGTTTGATGTTCGACTACCTGATGTTACAACATCCTGTGTTGAAGTTGAATTCCTAGATCTCAAGACTGCAAACCCAAATGTACGAAAAGATCAACAACCCTTGTTCGAAAGAGAAAGTATCCACACAGACGCTACAATCAAGCTCGTATCATCAGATAAAATCATAAGAGTTCATCTAGCTGTTCTTGCTGCCCGGTCACCTATGTTTGAATATATGTTTGCACAAGATTCTAAGGAGAATAACATGTCTGTTATTAACATCTCAGATATGTCACCTGAAGTTTGTCAAGCATTCATAGATTATCTATATGACAACATCGACGATGAAGAACTTGCGAATCATAGTTTCGAGCTATTGGTTGCTGCTGAGAAATACGGCGTTAATGATTTGAGAGAGGAGTGTGAAAAGAGTCTTCAAGAAGATATCAACAGAAAAAATATACTTGAGAGGCTCCATGCTGCATATTTCTATCAATTGCCCTACTTGAAGATTAGTTGCGCTCAATATCTAGTGGAATTTGGTAAAATACATGAAATCTGGGATGATTTTCTTGCCTTCCTAAAATTGGCAGATGATGATGTAATTTGCGAAATTTTCTTAGTTAAACTAGGTTTGAGTACCTTTCAGTTTTGA

Protein sequence

>MsaT029709.1

MNHFLDDDGPLSKNEVVDLVESVGYVAQWRLSFKSMNMIPHSFIHTSEAFKISKWNWHISFSRDSLSSPVTIKLYAEESTNNPPIASFKFRILDSIGKNGITFAETEEKDKLFTSETDAFNWEFDVRLPDVTTSCVEVEFLDLKTANPNVRKDQQPLFERESIHTDATIKLVSSDKIIRVHLAVLAARSPMFEYMFAQDSKENNMSVINISDMSPEVCQAFIDYLYDNIDDEELANHSFELLVAAEKYGVNDLREECEKSLQEDINRKNILERLHAAYFYQLPYLKISCAQYLVEFGKIHEIWDDFLAFLKLADDDVICEIFLVKLGLSTFQF*