AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029759

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029759 MtrunA17_Chr5g0447641 97.761 1161 26 0 1 1161 56 1216 0.0 2372
MsaG029759 MtrunA17_Chr3g0122391 55.681 977 376 15 204 1157 82 1024 0.0 1046
MsaG029759 MtrunA17_Chr8g0377481 53.387 989 403 11 188 1157 18 967 0.0 1034
MsaG029759 MtrunA17_Chr8g0392301 53.571 980 399 13 208 1157 41 994 0.0 975
MsaG029759 MtrunA17_Chr2g0311191 43.949 314 76 5 365 678 21 234 5.12e-66 223
MsaG029759 MtrunA17_Chr3g0136601 34.035 285 165 7 565 837 373 646 1.32e-35 147
MsaG029759 MtrunA17_Chr3g0136601 35.115 131 77 2 1020 1143 805 934 3.52e-15 80.9
MsaG029759 MtrunA17_Chr1g0150511 33.443 305 169 7 565 837 379 681 1.57e-34 143
MsaG029759 MtrunA17_Chr1g0150511 34.109 129 78 2 1021 1143 870 997 3.46e-13 74.3
MsaG029759 MtrunA17_Chr8g0371601 27.341 534 311 16 517 1011 426 921 1.25e-33 140
MsaG029759 MtrunA17_Chr8g0371601 31.944 144 61 4 1020 1145 1030 1154 5.63e-12 70.5
MsaG029759 MtrunA17_Chr5g0405701 31.288 326 190 6 517 833 464 764 8.35e-33 138
MsaG029759 MtrunA17_Chr5g0405701 32.164 171 66 5 1001 1145 1038 1184 1.93e-13 75.5
MsaG029759 MtrunA17_Chr4g0013831 26.895 409 246 6 556 950 338 707 1.63e-28 123
MsaG029759 MtrunA17_Chr7g0269631 30.389 283 158 5 556 837 338 582 5.65e-25 111
MsaG029759 MtrunA17_Chr1g0197591 25.948 343 227 8 521 851 335 662 2.09e-20 97.8
MsaG029759 MtrunA17_Chr1g0197591 33.333 177 88 5 984 1153 892 1045 5.52e-14 77.0
MsaG029759 MtrunA17_Chr1g0194741 25.697 323 192 11 521 818 352 651 6.02e-19 92.8
MsaG029759 MtrunA17_Chr1g0186431 22.103 466 305 12 541 1000 344 757 4.15e-13 73.9
MsaG029759 MtrunA17_Chr7g0274831 20.680 353 232 7 549 897 362 670 7.94e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029759 AT5G35750.1 59.534 1159 409 14 15 1157 59 1173 0.0 1321
MsaG029759 AT1G27320.1 55.765 954 383 11 221 1162 107 1033 0.0 1043
MsaG029759 AT2G01830.5 53.381 976 397 16 207 1157 118 1060 0.0 975
MsaG029759 AT2G01830.1 53.381 976 397 16 207 1157 106 1048 0.0 974
MsaG029759 AT2G01830.4 53.381 976 397 16 207 1157 106 1048 0.0 974
MsaG029759 AT2G01830.2 53.381 976 397 16 207 1157 129 1071 0.0 974
MsaG029759 AT2G01830.6 53.381 976 397 16 207 1157 106 1048 0.0 974
MsaG029759 AT2G01830.3 53.381 976 397 16 207 1157 106 1048 0.0 974
MsaG029759 AT5G10720.1 35.689 283 149 6 565 847 369 618 4.91e-40 161
MsaG029759 AT5G10720.1 34.615 130 78 3 1020 1143 779 907 1.79e-13 75.9
MsaG029759 AT2G17820.1 31.098 328 188 6 517 833 453 753 2.56e-35 147
MsaG029759 AT1G66340.1 30.460 348 193 6 556 897 337 641 1.21e-33 140
MsaG029759 AT2G40940.1 32.155 283 153 6 556 837 337 581 1.91e-24 110
MsaG029759 AT2G47430.1 27.273 308 177 10 551 837 384 665 5.15e-22 103
MsaG029759 AT2G47430.1 34.483 174 72 7 995 1146 956 1109 3.58e-13 75.1
MsaG029759 AT3G23150.1 24.869 382 227 12 559 923 364 702 4.13e-12 71.2
MsaG029759 AT3G04580.2 32.639 144 76 4 1020 1162 643 766 2.17e-11 68.9
MsaG029759 AT3G04580.1 32.639 144 76 4 1020 1162 643 766 2.17e-11 68.9

Find 239 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTCCTTGGACAGCATTC+TGG 0.103589 5:+81007121 MsaT029759.1:CDS
ATTAATTACCCGTCCATTTC+TGG 0.122771 5:-81009429 None:intergenic
ATTGATACACAAACGAATTT+TGG 0.159316 5:+81009254 MsaT029759.1:CDS
CCCTTTGGCCTCTGAGTTCC+AGG 0.193078 5:+81008673 MsaT029759.1:CDS
GTTTGACTGCAGAAAATATT+TGG 0.207225 5:+81005905 MsaT029759.1:intron
GGATGTTGTTTGGTCCTTAT+TGG 0.219358 5:+81005324 MsaT029759.1:CDS
TTGGACTTTAGTAGTTTAAA+AGG 0.222874 5:-81006418 None:intergenic
AAAGTTTCAAAAGTTAAATT+TGG 0.231203 5:+81008358 MsaT029759.1:CDS
GTTAATGTGTCCGAAGAATT+TGG 0.236764 5:+81004461 MsaT029759.1:CDS
CTTTAGCTCCTTCTGCTCAT+TGG 0.237386 5:-81009100 None:intergenic
AATTTATCTAGTTTAATTAA+AGG 0.239445 5:+81005303 MsaT029759.1:CDS
TGTTTGGTAACATTTCTTAT+TGG 0.260483 5:+81009616 MsaT029759.1:CDS
TTTAGCTCCTTCTGCTCATT+GGG 0.261024 5:-81009099 None:intergenic
CACGTAAGCGGTCTAGATTT+TGG 0.265877 5:+81006969 MsaT029759.1:CDS
ACAGCATTCTGGGCATCCTT+GGG 0.276319 5:+81007132 MsaT029759.1:CDS
GACCTCATGGGAGGAGAAAT+TGG 0.278166 5:+81008551 MsaT029759.1:CDS
TCCATGTATTGGAAGGTATC+TGG 0.278596 5:+81006797 MsaT029759.1:intron
TTTGTTATTACCTTTCTTCT+TGG 0.283122 5:+81007159 MsaT029759.1:CDS
TAGGAAACAGCTTCATGTTT+TGG 0.295445 5:+81004008 MsaT029759.1:CDS
ACCCGTCCATTTCTGGCATT+TGG 0.297045 5:-81009422 None:intergenic
TTGGTTCTGCATGGTTATTT+AGG 0.298048 5:+81005529 MsaT029759.1:CDS
ACCACTCGTGTGAGCAGTTT+GGG 0.298376 5:-81007708 None:intergenic
GTGGCACGTGTCTTCAGTTT+TGG 0.304174 5:+81009759 MsaT029759.1:CDS
TTTCAGTTCATTCACAATAA+AGG 0.310106 5:+81008173 MsaT029759.1:intron
GACAGCATTCTGGGCATCCT+TGG 0.312293 5:+81007131 MsaT029759.1:CDS
CAAATAATTACCAATCTTGT+TGG 0.322920 5:+81008055 MsaT029759.1:CDS
TGAACAAAGTCATCAAGAAT+TGG 0.326747 5:-81004531 None:intergenic
AAGTACAACACCGAGGTAAT+TGG 0.329193 5:-81006437 None:intergenic
GGGTGGTAAGGCTGCTCTTA+AGG 0.331631 5:+81009356 MsaT029759.1:CDS
GAAGGTGTTCTGCGAAAATA+TGG 0.332772 5:+81009312 MsaT029759.1:CDS
TCTGAAAAGTCCGATGAAAA+AGG 0.335369 5:+81007854 MsaT029759.1:CDS
TTCTTATAGACAAGGATTCT+TGG 0.335599 5:+81008968 MsaT029759.1:CDS
TGGTTGGACAATTAAGAAAA+TGG 0.343061 5:+81006030 MsaT029759.1:CDS
GACTAGGCTAATCCGGTATA+TGG 0.343716 5:+81009545 MsaT029759.1:CDS
CGACAGTTTCACATGAGATT+AGG 0.345247 5:+81007516 MsaT029759.1:CDS
ATTTCCTTGGACAGCATTCT+GGG 0.350113 5:+81007122 MsaT029759.1:CDS
TGGACTTTAGTAGTTTAAAA+GGG 0.352298 5:-81006417 None:intergenic
ATTATTTGTCGGAAGCGTTT+AGG 0.353184 5:-81008040 None:intergenic
CGCTGTTCAGGCGTAGCATC+TGG 0.360966 5:-81006490 None:intergenic
TTTGTAAGTGAGCCTGAAAT+TGG 0.376302 5:+81008575 MsaT029759.1:CDS
TACCACTCGTGTGAGCAGTT+TGG 0.378780 5:-81007709 None:intergenic
ATGTACTAATGAAGCCTCTT+TGG 0.380444 5:+81009142 MsaT029759.1:CDS
CCTCCTTTCATCAATACAAT+TGG 0.381731 5:-81004397 None:intergenic
TTACCTAAAACACCATTCAT+TGG 0.382326 5:-81007542 None:intergenic
CAGAATGCTGTCCAAGGAAA+TGG 0.384642 5:-81007120 None:intergenic
TGAAAGTTCTCCATTCTGAT+AGG 0.384878 5:+81005987 MsaT029759.1:CDS
CGATGACAACTTCAGGTACT+TGG 0.385643 5:-81008012 None:intergenic
TTTATGTCAGTTGGAATCCT+TGG 0.387171 5:+81005510 MsaT029759.1:CDS
TGGGGCTAAGGTGGGTGGCC+AGG 0.387928 5:-81009080 None:intergenic
TCGGTACCATACATCGTAAT+AGG 0.388024 5:-81006924 None:intergenic
GGATCTCCAAATGCCAGAAA+TGG 0.388575 5:+81009416 MsaT029759.1:CDS
TTTAATTAAAGGATGTTGTT+TGG 0.394773 5:+81005314 MsaT029759.1:CDS
AATTGCTTGTGGGAAAGAAT+TGG 0.395615 5:+81009587 MsaT029759.1:CDS
TCTTATAGACAAGGATTCTT+GGG 0.395654 5:+81008969 MsaT029759.1:CDS
TCAAAAGCAGAAGGTAGTTA+AGG 0.396948 5:+81005395 MsaT029759.1:CDS
TGAAGAAGAGCAACTCTATA+CGG 0.397116 5:+81009734 MsaT029759.1:CDS
TCTTGTTGGAAATTCACTTA+AGG 0.397528 5:+81008069 MsaT029759.1:CDS
TACAGAGAATCCCTTGGAAC+TGG 0.398252 5:+81009186 MsaT029759.1:CDS
TCGCGAACATACGGTGGAAC+TGG 0.403424 5:+81008497 MsaT029759.1:CDS
ACATACGGTGGAACTGGAAT+AGG 0.407969 5:+81008503 MsaT029759.1:CDS
GGTACCGATGTTCTTGATAC+TGG 0.414059 5:+81006939 MsaT029759.1:CDS
AGCATACGCCTCCATTTCCT+TGG 0.415014 5:+81007109 MsaT029759.1:CDS
AAAGAATTGGCAGAGATGTT+TGG 0.417574 5:+81009600 MsaT029759.1:CDS
CGAGCAAGGGCATCTGGAAA+GGG 0.420999 5:+81006382 MsaT029759.1:CDS
AGAATGCTTAAACTTGAATC+AGG 0.423838 5:+81008268 MsaT029759.1:CDS
AAGTGAATTTCCAACAAGAT+TGG 0.429542 5:-81008065 None:intergenic
GGGACAGTATAATGCCAATA+AGG 0.430051 5:-81005338 None:intergenic
ATACTGTCCCACAAGTTGTC+TGG 0.431443 5:+81005351 MsaT029759.1:CDS
GAACAAAGTCATCAAGAATT+GGG 0.433982 5:-81004530 None:intergenic
TAGAGTTGCTCTTCTTCAAA+TGG 0.434245 5:-81009729 None:intergenic
TAAATTGATCTTGCAACATA+CGG 0.441964 5:-81005617 None:intergenic
GACAGCAATGGTTTGCTGAC+AGG 0.443901 5:-81006871 None:intergenic
CACAAGTGATGGAACATCAT+AGG 0.445281 5:-81006826 None:intergenic
AGCTTGGATTATCATTCCCT+TGG 0.446435 5:+81004559 MsaT029759.1:CDS
CTGCTCATTGGGGCTAAGGT+GGG 0.446711 5:-81009088 None:intergenic
GTATCCATCGACATGATGTC+AGG 0.447012 5:+81006166 MsaT029759.1:CDS
CCTTCTGCTCATTGGGGCTA+AGG 0.447909 5:-81009092 None:intergenic
GGATCGCCGATGACAACTTC+AGG 0.448585 5:-81008019 None:intergenic
GACTTTGTTCACATGAAGCT+TGG 0.449258 5:+81004543 MsaT029759.1:CDS
GTAGCTTCAATACGCTGTTC+AGG 0.450753 5:-81006502 None:intergenic
TGCAGATGTCGCAAAATCTC+AGG 0.453763 5:+81007269 MsaT029759.1:CDS
GAAGAATTTGGAGTTACAAA+AGG 0.453998 5:+81004473 MsaT029759.1:CDS
TAGGAAACAGATGGAAAAGC+TGG 0.456035 5:+81008333 MsaT029759.1:CDS
TCTGATCCTATTACGATGTA+TGG 0.457715 5:+81006918 MsaT029759.1:CDS
GATGAGGGTGTGTGCGATTG+TGG 0.459371 5:+81005272 MsaT029759.1:CDS
CAAGATTGGTAATTATTTGT+CGG 0.462240 5:-81008051 None:intergenic
TGGAGAATATACAGAGAGTT+CGG 0.464515 5:+81005925 MsaT029759.1:CDS
TTGAGGTGACTAGGCTAATC+CGG 0.464950 5:+81009538 MsaT029759.1:CDS
ACCAGTAGCCTTTGAATGAC+CGG 0.467332 5:-81005460 None:intergenic
TGGTGCATATTCATCTTGAA+TGG 0.472744 5:-81006110 None:intergenic
TCTGCTCATTGGGGCTAAGG+TGG 0.472855 5:-81009089 None:intergenic
CTCATTGGGGCTAAGGTGGG+TGG 0.473446 5:-81009085 None:intergenic
AGAATGTATTCCAGAAAACT+TGG 0.474067 5:+81006078 MsaT029759.1:CDS
AGGTATGCTGCAAATGTTGA+TGG 0.474230 5:+81007652 MsaT029759.1:intron
GTTTCTTGAGCAAATACAAC+TGG 0.474794 5:-81006130 None:intergenic
ATCAAAACACTTTGCCAAAG+AGG 0.475890 5:-81009156 None:intergenic
CATATTTACACCTTTCATGC+AGG 0.477916 5:+81008460 MsaT029759.1:CDS
ATCCTTGTCTATAAGAATCA+TGG 0.478300 5:-81008962 None:intergenic
CCATGTATTGGAAGGTATCT+GGG 0.482800 5:+81006798 MsaT029759.1:intron
GCGAGCAAGGGCATCTGGAA+AGG 0.484052 5:+81006381 MsaT029759.1:CDS
CTTGTTCTCAGGATCAGATC+AGG 0.487332 5:+81004035 MsaT029759.1:CDS
CAAGGACAGAATGGCGTCAA+AGG 0.488603 5:+81009027 MsaT029759.1:CDS
TTACCTCGTTCTCCATATAC+CGG 0.488691 5:-81009557 None:intergenic
AATCCATCTGATTGTCATCA+AGG 0.489725 5:-81007682 None:intergenic
CCCGGTCATTCAAAGGCTAC+TGG 0.491515 5:+81005459 MsaT029759.1:CDS
AATGTTACATATGCTTTCCA+AGG 0.494279 5:-81004576 None:intergenic
AATAAATCGAATAGCTAAAG+TGG 0.498068 5:+81007200 MsaT029759.1:CDS
ACGATATCACCTGCAGAGGT+TGG 0.499162 5:+81008745 MsaT029759.1:CDS
CCTCGACTGTTACTAGTAGT+TGG 0.500006 5:-81008400 None:intergenic
ACAGTCGAGGACACTGGTAT+AGG 0.500734 5:+81008413 MsaT029759.1:CDS
TGTCCAATTGTATTGATGAA+AGG 0.501841 5:+81004394 MsaT029759.1:CDS
CAGCATTCTGGGCATCCTTG+GGG 0.503392 5:+81007133 MsaT029759.1:CDS
AACAAAAGCCAGACAACTTG+TGG 0.503467 5:-81005359 None:intergenic
CGATATCACCTGCAGAGGTT+GGG 0.504786 5:+81008746 MsaT029759.1:CDS
ACATCCTTCCGCAGTTGACC+AGG 0.506511 5:+81005701 MsaT029759.1:CDS
TTACTAATGCTCTCAAACCC+TGG 0.510047 5:-81008691 None:intergenic
CGAATTCAACATTGTATGCC+CGG 0.511311 5:+81005441 MsaT029759.1:CDS
TTGGAATCCTTGGTTCTGCA+TGG 0.513364 5:+81005520 MsaT029759.1:CDS
GAAGAGTCCTCTTCACATCA+TGG 0.514625 5:+81008232 MsaT029759.1:CDS
CAAAATGCATCCTATCAGAA+TGG 0.515566 5:-81005997 None:intergenic
AGTTCCACATCGCGAACATA+CGG 0.515602 5:+81008488 MsaT029759.1:CDS
TACCTGTGTGGAGGGTGGTA+AGG 0.516659 5:+81009344 MsaT029759.1:CDS
AGTTGTATTTGCTCAAGAAA+CGG 0.523581 5:+81006132 MsaT029759.1:CDS
GTGGCAGAAGAATAACCCTT+TGG 0.524349 5:+81008658 MsaT029759.1:CDS
GAGCAAAACGTTAACCAAAT+CGG 0.525792 5:-81004616 None:intergenic
CCTGGAACTCAGAGGCCAAA+GGG 0.527638 5:-81008673 None:intergenic
CAGACATGCCCAACCTCTGC+AGG 0.530444 5:-81008754 None:intergenic
GGACTTTAGTAGTTTAAAAG+GGG 0.531138 5:-81006416 None:intergenic
GGTGCATATTCATCTTGAAT+GGG 0.531343 5:-81006109 None:intergenic
CTGATCTGATCCTGAGAACA+AGG 0.533568 5:-81004034 None:intergenic
AAATTGATCTTGCAACATAC+GGG 0.535060 5:-81005616 None:intergenic
GAGTGGTAAAGATCTTATAT+CGG 0.537674 5:+81007724 MsaT029759.1:CDS
GGCTCTCTCATCACACATAG+TGG 0.539504 5:-81005595 None:intergenic
CGCTACTACAGAGAATCCCT+TGG 0.539625 5:+81009180 MsaT029759.1:CDS
TATTCATCTTGAATGGGCGC+TGG 0.540279 5:-81006103 None:intergenic
TCCAAATGCCAGAAATGGAC+GGG 0.542668 5:+81009421 MsaT029759.1:CDS
CCTTTGGCCTCTGAGTTCCA+GGG 0.543146 5:+81008674 MsaT029759.1:CDS
ATCAAGAAACAAGGACAGAA+TGG 0.546253 5:+81009018 MsaT029759.1:CDS
CTTCAAATGTTGTTAAGTAG+TGG 0.546319 5:+81004420 MsaT029759.1:CDS
TAAATGTCTAGTTGACCTCA+TGG 0.554358 5:+81008538 MsaT029759.1:CDS
AGAAGCTTGTCCACAAGTGA+TGG 0.554573 5:-81006837 None:intergenic
TCAGCTGTCATAGCTAGTAT+TGG 0.556557 5:-81009645 None:intergenic
GGCCATGATTCTTATAGACA+AGG 0.556608 5:+81008960 MsaT029759.1:CDS
TTTCAGTATGTCGACGCAGT+TGG 0.556794 5:+81008939 MsaT029759.1:intron
CCAATTGTATTGATGAAAGG+AGG 0.556888 5:+81004397 MsaT029759.1:CDS
AAATATATATCCAAGAAGAA+AGG 0.557028 5:-81007169 None:intergenic
CTATGATGTTCCATCACTTG+TGG 0.557856 5:+81006827 MsaT029759.1:CDS
AAACAAGCATCAAAATTATG+TGG 0.560851 5:-81009390 None:intergenic
TTGGAAAGCATATGTAACAT+TGG 0.562808 5:+81004578 MsaT029759.1:CDS
CCAGTAGCCTTTGAATGACC+GGG 0.563893 5:-81005459 None:intergenic
GTCATACACATTGACAGCAA+TGG 0.567089 5:-81006883 None:intergenic
TAAGCAACACCACTAGTAAG+TGG 0.568808 5:-81005959 None:intergenic
AAAGCAAGCAGATTGAAGAC+TGG 0.571842 5:-81008789 None:intergenic
ACAAAAGCCAGACAACTTGT+GGG 0.571903 5:-81005358 None:intergenic
CCCTGGAACTCAGAGGCCAA+AGG 0.573447 5:-81008674 None:intergenic
ACACAAGAATAGCGAGAGCA+TGG 0.573638 5:-81005659 None:intergenic
CAGAAGGAGCTAAAGTCAGT+TGG 0.574474 5:+81009108 MsaT029759.1:CDS
AAATATGCGGCTTTGAGCAT+CGG 0.576359 5:-81008444 None:intergenic
TTGTATGCCCGGTCATTCAA+AGG 0.577652 5:+81005452 MsaT029759.1:CDS
AAAAGGAGAAACAGAATCTG+TGG 0.578251 5:+81008628 MsaT029759.1:CDS
GAGATTAGGACTCCAATGAA+TGG 0.579290 5:+81007530 MsaT029759.1:CDS
TATCAAGCTAAGATCGAGGC+AGG 0.579435 5:+81007764 MsaT029759.1:CDS
GAACTATCAGCCTGCATGAA+AGG 0.579814 5:-81008470 None:intergenic
GAAAACATGAGATGCACTGC+AGG 0.579994 5:+81007003 MsaT029759.1:CDS
AGAACATGCAATACATTGAG+TGG 0.580850 5:+81008302 MsaT029759.1:CDS
CCTTAGCCCCAATGAGCAGA+AGG 0.581226 5:+81009092 MsaT029759.1:CDS
CTCCAAATGCCAGAAATGGA+CGG 0.581688 5:+81009420 MsaT029759.1:CDS
TGGTTTGCTGACAGGCAAGC+TGG 0.582491 5:-81006863 None:intergenic
TTAGCTCCTTCTGCTCATTG+GGG 0.582725 5:-81009098 None:intergenic
GTCATTCAAAGGCTACTGGT+TGG 0.583595 5:+81005463 MsaT029759.1:CDS
CAGCGTATTGAAGCTACTGT+GGG 0.584573 5:+81006508 MsaT029759.1:CDS
ATTCAAAGGCTACTGGTTGG+CGG 0.585519 5:+81005466 MsaT029759.1:CDS
ACATTGAGTGGATTTCCTGT+AGG 0.587511 5:+81008314 MsaT029759.1:CDS
TTTCTGATACTTGCAACTCA+AGG 0.587800 5:+81008816 MsaT029759.1:CDS
AAGGAAGAATCAAAAGCAGA+AGG 0.589976 5:+81005386 MsaT029759.1:CDS
GATTTCCTGTAGGAAACAGA+TGG 0.596201 5:+81008324 MsaT029759.1:CDS
GATGCCCAGAATGCTGTCCA+AGG 0.597141 5:-81007126 None:intergenic
TGACCTTGATGACAATCAGA+TGG 0.600124 5:+81007679 MsaT029759.1:CDS
GCAGTAGTTACCTGTGTGGA+GGG 0.602358 5:+81009336 MsaT029759.1:CDS
GAATAGCGAGAGCATGGACA+TGG 0.603489 5:-81005653 None:intergenic
CAGAATCTGTGGATGAGAAG+TGG 0.603636 5:+81008639 MsaT029759.1:CDS
AGCAAGGGCATCTGGAAAGG+GGG 0.603947 5:+81006384 MsaT029759.1:CDS
ATGTTACATATGCTTTCCAA+GGG 0.605409 5:-81004575 None:intergenic
AGACAAGGATTCTTGGGACA+AGG 0.605502 5:+81008975 MsaT029759.1:CDS
GAGCAAGGGCATCTGGAAAG+GGG 0.605827 5:+81006383 MsaT029759.1:CDS
CTTCCCTGACATCATGTCGA+TGG 0.606250 5:-81006170 None:intergenic
AAATGTCTAGTTGACCTCAT+GGG 0.606651 5:+81008539 MsaT029759.1:CDS
AAACTTGAATCAGGACATGT+CGG 0.606659 5:+81008277 MsaT029759.1:CDS
TTTGCAATCACAGGTGATGA+GGG 0.607181 5:+81005257 MsaT029759.1:intron
ACAGAGAATCCCTTGGAACT+GGG 0.608161 5:+81009187 MsaT029759.1:CDS
AGCATTCTGGGCATCCTTGG+GGG 0.608710 5:+81007134 MsaT029759.1:CDS
CAAGTACCTGAAGTTGTCAT+CGG 0.609914 5:+81008013 MsaT029759.1:CDS
GACATTGTAACGTTGTAACA+AGG 0.610027 5:-81003983 None:intergenic
ACTGCATCCATGATGTGAAG+AGG 0.611159 5:-81008239 None:intergenic
GCCCAAACTGCTCACACGAG+TGG 0.611642 5:+81007707 MsaT029759.1:CDS
TCCACCGTATGTTCGCGATG+TGG 0.613281 5:-81008492 None:intergenic
GTGAACAGAAAAGTTGCAGA+AGG 0.613404 5:+81009294 MsaT029759.1:CDS
AGGAAACAGATGGAAAAGCT+GGG 0.614483 5:+81008334 MsaT029759.1:CDS
TCTTATATCGGTTATAAGCG+AGG 0.615675 5:+81007736 MsaT029759.1:CDS
CAAGCATCAAAATTATGTGG+TGG 0.622634 5:-81009387 None:intergenic
CTAGTAACAGTCGAGGACAC+TGG 0.623583 5:+81008407 MsaT029759.1:CDS
TCCACATCGCGAACATACGG+TGG 0.623794 5:+81008491 MsaT029759.1:CDS
TATCCATCGACATGATGTCA+GGG 0.623915 5:+81006167 MsaT029759.1:CDS
GTTACCTGGTCAACTGCGGA+AGG 0.629231 5:-81005705 None:intergenic
ATGCGGCTTTGAGCATCGGT+AGG 0.631149 5:-81008440 None:intergenic
CTTGTGTCTACATTTCACCA+TGG 0.631262 5:+81005675 MsaT029759.1:CDS
ACAGCGTATTGAAGCTACTG+TGG 0.633680 5:+81006507 MsaT029759.1:CDS
TAAATAACCATGCAGAACCA+AGG 0.637583 5:-81005527 None:intergenic
GGCAGGCAAACTAGAACTTG+AGG 0.639131 5:+81007781 MsaT029759.1:CDS
AGCCTTACCACCCTCCACAC+AGG 0.641150 5:-81009346 None:intergenic
AGAGCAACTCTATACGGCAG+TGG 0.641157 5:+81009740 MsaT029759.1:CDS
CTACTAAAGTCCAATTACCT+CGG 0.645725 5:+81006427 MsaT029759.1:CDS
TTCTTCGGACACATTAACTG+TGG 0.646243 5:-81004456 None:intergenic
AATGCTGTCCAAGGAAATGG+AGG 0.646257 5:-81007117 None:intergenic
TACAACGTTACAATGTCAGT+AGG 0.648876 5:+81003989 MsaT029759.1:CDS
GATATCGTGTAACCTCAGCC+CGG 0.651457 5:-81008730 None:intergenic
CATCGACATGATGTCAGGGA+AGG 0.652451 5:+81006171 MsaT029759.1:CDS
TGTTCAGGCGTAGCATCTGG+AGG 0.656731 5:-81006487 None:intergenic
GCATGAAAGGTGTAAATATG+CGG 0.658298 5:-81008457 None:intergenic
TGTCTAGTTGACCTCATGGG+AGG 0.658386 5:+81008542 MsaT029759.1:CDS
AGCAGTAGTTACCTGTGTGG+AGG 0.662067 5:+81009335 MsaT029759.1:CDS
TTTGAGAGACCACTTACTAG+TGG 0.664415 5:+81005950 MsaT029759.1:CDS
GAAAATGGAAACTGAGAACG+AGG 0.669396 5:+81006045 MsaT029759.1:CDS
ACTTCATCTAGAATTGCATG+TGG 0.670385 5:-81007818 None:intergenic
GTAGTTACCTGTGTGGAGGG+TGG 0.673674 5:+81009339 MsaT029759.1:CDS
CTGCGGAAGGATGTTTACCA+TGG 0.679151 5:-81005692 None:intergenic
ACTGGCTTACTACACGTAAG+CGG 0.680944 5:+81006957 MsaT029759.1:CDS
TAAGCCAGTATCAAGAACAT+CGG 0.681711 5:-81006943 None:intergenic
ATCCAATTTCTCCTCCCATG+AGG 0.687075 5:-81008553 None:intergenic
TTATACGCAATCAAGAAACA+AGG 0.692490 5:+81009009 MsaT029759.1:CDS
TTACACGATATCACCTGCAG+AGG 0.694581 5:+81008741 MsaT029759.1:CDS
TCTCAAACCCTGGAACTCAG+AGG 0.698392 5:-81008681 None:intergenic
TCTCTATCAAGCTAAGATCG+AGG 0.707670 5:+81007760 MsaT029759.1:CDS
TGGAGCAGTAGTTACCTGTG+TGG 0.714398 5:+81009332 MsaT029759.1:CDS
GTATGTTACCTGGTCAACTG+CGG 0.716416 5:-81005709 None:intergenic
CCAACTACTAGTAACAGTCG+AGG 0.735761 5:+81008400 MsaT029759.1:CDS
AATCCGGTATATGGAGAACG+AGG 0.748717 5:+81009554 MsaT029759.1:CDS
AGCGTATTGAAGCTACTGTG+GGG 0.753444 5:+81006509 MsaT029759.1:CDS
CAAATGTAAGTACAACACCG+AGG 0.806793 5:-81006444 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 81003919 81009791 81003919 ID=MsaG029759
Chr5 mRNA 81003919 81009791 81003919 ID=MsaT029759.1;Parent=MsaG029759
Chr5 exon 81003919 81004056 81003919 ID=MsaT029759.1.exon1;Parent=MsaT029759.1
Chr5 CDS 81003919 81004056 81003919 ID=cds.MsaT029759.1;Parent=MsaT029759.1
Chr5 exon 81004390 81004647 81004390 ID=MsaT029759.1.exon2;Parent=MsaT029759.1
Chr5 CDS 81004390 81004647 81004390 ID=cds.MsaT029759.1;Parent=MsaT029759.1
Chr5 exon 81005270 81005722 81005270 ID=MsaT029759.1.exon3;Parent=MsaT029759.1
Chr5 CDS 81005270 81005722 81005270 ID=cds.MsaT029759.1;Parent=MsaT029759.1
Chr5 exon 81005917 81006192 81005917 ID=MsaT029759.1.exon4;Parent=MsaT029759.1
Chr5 CDS 81005917 81006192 81005917 ID=cds.MsaT029759.1;Parent=MsaT029759.1
Chr5 exon 81006361 81006530 81006361 ID=MsaT029759.1.exon5;Parent=MsaT029759.1
Chr5 CDS 81006361 81006530 81006361 ID=cds.MsaT029759.1;Parent=MsaT029759.1
Chr5 exon 81006812 81007024 81006812 ID=MsaT029759.1.exon6;Parent=MsaT029759.1
Chr5 CDS 81006812 81007024 81006812 ID=cds.MsaT029759.1;Parent=MsaT029759.1
Chr5 exon 81007104 81007290 81007104 ID=MsaT029759.1.exon7;Parent=MsaT029759.1
Chr5 CDS 81007104 81007290 81007104 ID=cds.MsaT029759.1;Parent=MsaT029759.1
Chr5 exon 81007509 81007560 81007509 ID=MsaT029759.1.exon8;Parent=MsaT029759.1
Chr5 CDS 81007509 81007560 81007509 ID=cds.MsaT029759.1;Parent=MsaT029759.1
Chr5 exon 81007654 81007883 81007654 ID=MsaT029759.1.exon9;Parent=MsaT029759.1
Chr5 CDS 81007654 81007883 81007654 ID=cds.MsaT029759.1;Parent=MsaT029759.1
Chr5 exon 81007992 81008090 81007992 ID=MsaT029759.1.exon10;Parent=MsaT029759.1
Chr5 CDS 81007992 81008090 81007992 ID=cds.MsaT029759.1;Parent=MsaT029759.1
Chr5 exon 81008179 81008837 81008179 ID=MsaT029759.1.exon11;Parent=MsaT029759.1
Chr5 CDS 81008179 81008837 81008179 ID=cds.MsaT029759.1;Parent=MsaT029759.1
Chr5 exon 81008945 81009442 81008945 ID=MsaT029759.1.exon12;Parent=MsaT029759.1
Chr5 CDS 81008945 81009442 81008945 ID=cds.MsaT029759.1;Parent=MsaT029759.1
Chr5 exon 81009536 81009791 81009536 ID=MsaT029759.1.exon13;Parent=MsaT029759.1
Chr5 CDS 81009536 81009791 81009536 ID=cds.MsaT029759.1;Parent=MsaT029759.1
Gene Sequence

>MsaT029759.1

ATGTTTAGTACTTTCAACTTTAAGCATTTGATTAAAGAAACAGCTTGTGAAAAGAGTACACAAACCTTGTTACAACGTTACAATGTCAGTAGGAAACAGCTTCATGTTTTGGCTTCCTTGTTCTCAGGATCAGATCAGATTTTGTCCAATTGTATTGATGAAAGGAGGCTTCAAATGTTGTTAAGTAGTGGTACAGTGAACACTCCACAGTTAATGTGTCCGAAGAATTTGGAGTTACAAAAGGAGCATAGATATGTTGCAGAATCTGTAGAACAATGCCCAATTCTTGATGACTTTGTTCACATGAAGCTTGGATTATCATTCCCTTGGAAAGCATATGTAACATTGGACTCTGCACTTTTGTCCGATTTGGTTAACGTTTTGCTCCGTGGAAAGGTGATGAGGGTGTGTGCGATTGTGGATAATGTGAATTTATCTAGTTTAATTAAAGGATGTTGTTTGGTCCTTATTGGCATTATACTGTCCCACAAGTTGTCTGGCTTTTGTTTACTAAGGAAGAATCAAAAGCAGAAGGTAGTTAAGGAGCATCCACCTGCTCCACAAAAGCGAATTCAACATTGTATGCCCGGTCATTCAAAGGCTACTGGTTGGCGGAGAAAAAAGTTGCTAGTTATTTTTATGTCAGTTGGAATCCTTGGTTCTGCATGGTTATTTAGGTACATGAGTTCAGACATTGCGCAAAGAAGAGAAGAAATGCTTGCCACTATGTGTGATGAGAGAGCCCGTATGTTGCAAGATCAATTTAACGTGAGCATGAACCATGTCCATGCTCTCGCTATTCTTGTGTCTACATTTCACCATGGTAAACATCCTTCCGCAGTTGACCAGAAAATATTTGGAGAATATACAGAGAGTTCGGCATTTGAGAGACCACTTACTAGTGGTGTTGCTTATGCTTTGAAAGTTCTCCATTCTGATAGGATGCATTTTGAGAAGCAGCATGGTTGGACAATTAAGAAAATGGAAACTGAGAACGAGGCATTAGTTCAAGAATGTATTCCAGAAAACTTGGATCCAGCGCCCATTCAAGATGAATATGCACCAGTTGTATTTGCTCAAGAAACGGTTTCTCATATTGTATCCATCGACATGATGTCAGGGAAGGAGGATCGTGAGAATATTTTGCGAGCAAGGGCATCTGGAAAGGGGGTTCTGACATCCCCTTTTAAACTACTAAAGTCCAATTACCTCGGTGTTGTACTTACATTTGCTGTCTATGACTCTAAGCTTCCTCCAGATGCTACGCCTGAACAGCGTATTGAAGCTACTGTGGGGTATCTGGGTGCATCCTATGATGTTCCATCACTTGTGGACAAGCTTCTTCACCAGCTTGCCTGTCAGCAAACCATTGCTGTCAATGTGTATGACACAACTAATGCATCTGATCCTATTACGATGTATGGTACCGATGTTCTTGATACTGGCTTACTACACGTAAGCGGTCTAGATTTTGGGGATCCACTACGAAAACATGAGATGCACTGCAGGTTCAAGCATACGCCTCCATTTCCTTGGACAGCATTCTGGGCATCCTTGGGGGTATTTGTTATTACCTTTCTTCTTGGATATATATTTTATGCAGCAATAAATCGAATAGCTAAAGTGGAAGATGACTATTGTCAAATGAGAGAGCTGAAAGTTCGTGCTGAAGCTGCAGATGTCGCAAAATCTCAGTTTCTTGCGACAGTTTCACATGAGATTAGGACTCCAATGAATGGTGTTTTAGGTATGCTGCAAATGTTGATGGATACTGACCTTGATGACAATCAGATGGATTTTGCCCAAACTGCTCACACGAGTGGTAAAGATCTTATATCGGTTATAAGCGAGGTTCTCTATCAAGCTAAGATCGAGGCAGGCAAACTAGAACTTGAGGCTGTAGCTTTTCATCCACATGCAATTCTAGATGAAGTTCTATCACTTTTCTCTGAAAAGTCCGATGAAAAAGGAATTGAGTTGGCTGTTTATGCATCTAACCAAGTACCTGAAGTTGTCATCGGCGATCCTAAACGCTTCCGACAAATAATTACCAATCTTGTTGGAAATTCACTTAAGTTCATTCACAATAAAGGACATGTTTTTGTCTCGATTCATCTGGCAAATGAAGTGAAGAGTCCTCTTCACATCATGGATGCAGTGCTGAGAGAATGCTTAAACTTGAATCAGGACATGTCGGAAAGAACATGCAATACATTGAGTGGATTTCCTGTAGGAAACAGATGGAAAAGCTGGGAAAAGTTTCAAAAGTTAAATTTGGCGCATGAGTCAGAAACTATCCAACTACTAGTAACAGTCGAGGACACTGGTATAGGAATTCCTACCGATGCTCAAAGCCGCATATTTACACCTTTCATGCAGGCTGATAGTTCCACATCGCGAACATACGGTGGAACTGGAATAGGATTAAGCATTAGTAAATGTCTAGTTGACCTCATGGGAGGAGAAATTGGATTTGTAAGTGAGCCTGAAATTGGAAGTACTTTTTCATTTACTGGAAATTTTAGAAAAGGAGAAACAGAATCTGTGGATGAGAAGTGGCAGAAGAATAACCCTTTGGCCTCTGAGTTCCAGGGTTTGAGAGCATTAGTAATAGATAGAAGAAAAATCCGGGCTGAGGTTACACGATATCACCTGCAGAGGTTGGGCATGTCTGTAGATGTAAATTCCAGTCTTCAATCTGCTTGCTTTTCTATTTCTGATACTTGCAACTCAAGTATGTCGACGCAGTTGGCCATGATTCTTATAGACAAGGATTCTTGGGACAAGGAAAGTAGCATCTTATACGCAATCAAGAAACAAGGACAGAATGGCGTCAAAGGTGACTCGCTAAACTTTCCAAAGATTTTTCTCCTGGCCACCCACCTTAGCCCCAATGAGCAGAAGGAGCTAAAGTCAGTTGGTATCATAGATGATGTACTAATGAAGCCTCTTTGGCAAAGTGTTTTGATTCGCTACTACAGAGAATCCCTTGGAACTGGGAGAAAACAAATAGACAGAAAAAAAGTATCCCAACTTGAAAATTTATTGATACACAAACGAATTTTGGTGGTCGATGATAACGCAGTGAACAGAAAAGTTGCAGAAGGTGTTCTGCGAAAATATGGAGCAGTAGTTACCTGTGTGGAGGGTGGTAAGGCTGCTCTTAAGGTGCTTAAGCCACCACATAATTTTGATGCTTGTTTCATGGATCTCCAAATGCCAGAAATGGACGGTTTTGAGGTGACTAGGCTAATCCGGTATATGGAGAACGAGGTAAATGAAAAAATTGCTTGTGGGAAAGAATTGGCAGAGATGTTTGGTAACATTTCTTATTGGCACATACCAATACTAGCTATGACAGCTGATGTAACTCAAGCATCAAATGAAGAATGCAAAAAATGTGGGATGGATGATTTTGTGTCAAAGCCATTTGAAGAAGAGCAACTCTATACGGCAGTGGCACGTGTCTTCAGTTTTGGTTCAGAGTAA
Protein sequence

>MsaT029759.1

MFSTFNFKHLIKETACEKSTQTLLQRYNVSRKQLHVLASLFSGSDQILSNCIDERRLQMLLSSGTVNTPQLMCPKNLELQKEHRYVAESVEQCPILDDFVHMKLGLSFPWKAYVTLDSALLSDLVNVLLRGKVMRVCAIVDNVNLSSLIKGCCLVLIGIILSHKLSGFCLLRKNQKQKVVKEHPPAPQKRIQHCMPGHSKATGWRRKKLLVIFMSVGILGSAWLFRYMSSDIAQRREEMLATMCDERARMLQDQFNVSMNHVHALAILVSTFHHGKHPSAVDQKIFGEYTESSAFERPLTSGVAYALKVLHSDRMHFEKQHGWTIKKMETENEALVQECIPENLDPAPIQDEYAPVVFAQETVSHIVSIDMMSGKEDRENILRARASGKGVLTSPFKLLKSNYLGVVLTFAVYDSKLPPDATPEQRIEATVGYLGASYDVPSLVDKLLHQLACQQTIAVNVYDTTNASDPITMYGTDVLDTGLLHVSGLDFGDPLRKHEMHCRFKHTPPFPWTAFWASLGVFVITFLLGYIFYAAINRIAKVEDDYCQMRELKVRAEAADVAKSQFLATVSHEIRTPMNGVLGMLQMLMDTDLDDNQMDFAQTAHTSGKDLISVISEVLYQAKIEAGKLELEAVAFHPHAILDEVLSLFSEKSDEKGIELAVYASNQVPEVVIGDPKRFRQIITNLVGNSLKFIHNKGHVFVSIHLANEVKSPLHIMDAVLRECLNLNQDMSERTCNTLSGFPVGNRWKSWEKFQKLNLAHESETIQLLVTVEDTGIGIPTDAQSRIFTPFMQADSSTSRTYGGTGIGLSISKCLVDLMGGEIGFVSEPEIGSTFSFTGNFRKGETESVDEKWQKNNPLASEFQGLRALVIDRRKIRAEVTRYHLQRLGMSVDVNSSLQSACFSISDTCNSSMSTQLAMILIDKDSWDKESSILYAIKKQGQNGVKGDSLNFPKIFLLATHLSPNEQKELKSVGIIDDVLMKPLWQSVLIRYYRESLGTGRKQIDRKKVSQLENLLIHKRILVVDDNAVNRKVAEGVLRKYGAVVTCVEGGKAALKVLKPPHNFDACFMDLQMPEMDGFEVTRLIRYMENEVNEKIACGKELAEMFGNISYWHIPILAMTADVTQASNEECKKCGMDDFVSKPFEEEQLYTAVARVFSFGSE*