AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029770 MtrunA17_Chr5g0447691 99.081 544 5 0 1 544 1 544 0.0 1131
MsaG029770 MtrunA17_Chr3g0101141 57.532 551 218 5 1 544 1 542 0.0 649
MsaG029770 MtrunA17_Chr5g0445331 56.937 555 205 8 1 544 1 532 0.0 619
MsaG029770 MtrunA17_Chr4g0076091 57.350 551 218 8 1 543 1 542 0.0 608
MsaG029770 MtrunA17_Chr2g0319091 63.824 387 137 1 157 543 195 578 0.0 526
MsaG029770 MtrunA17_Chr2g0290241 56.667 420 180 2 126 544 7 425 5.75e-174 498
MsaG029770 MtrunA17_Chr1g0159461 56.216 370 162 0 175 544 223 592 2.67e-159 466
MsaG029770 MtrunA17_Chr3g0125811 54.278 374 171 0 171 544 197 570 5.42e-151 444
MsaG029770 MtrunA17_Chr7g0224271 35.603 514 271 9 49 544 202 673 2.44e-87 283
MsaG029770 MtrunA17_Chr3g0110971 36.923 390 209 7 174 544 216 587 2.13e-73 244
MsaG029770 MtrunA17_Chr8g0376381 36.968 376 213 8 174 544 166 522 5.69e-72 239
MsaG029770 MtrunA17_Chr7g0238891 34.048 373 245 1 173 544 368 740 2.38e-69 237
MsaG029770 MtrunA17_Chr4g0031721 32.796 372 249 1 173 543 312 683 2.19e-65 225
MsaG029770 MtrunA17_Chr2g0328381 29.150 494 310 10 74 543 163 640 6.55e-65 223
MsaG029770 MtrunA17_Chr4g0031711 32.447 376 249 3 173 543 357 732 8.37e-65 224
MsaG029770 MtrunA17_Chr8g0357601 33.594 384 234 7 173 544 178 552 9.02e-65 221
MsaG029770 MtrunA17_Chr2g0306641 36.010 386 220 11 170 544 118 487 2.10e-64 218
MsaG029770 MtrunA17_Chr4g0031741 33.245 376 246 4 170 543 277 649 3.59e-64 221
MsaG029770 MtrunA17_Chr7g0236851 33.494 415 255 7 140 543 252 656 3.93e-63 218
MsaG029770 MtrunA17_Chr2g0328341 28.279 488 317 9 74 543 165 637 6.33e-62 214
MsaG029770 MtrunA17_Chr3g0085681 31.265 419 228 7 178 544 26 436 8.00e-61 207
MsaG029770 MtrunA17_Chr6g0468561 35.354 396 204 13 175 544 293 662 1.47e-60 211
MsaG029770 MtrunA17_Chr4g0038851 30.787 432 231 7 174 544 45 469 2.50e-60 206
MsaG029770 MtrunA17_Chr2g0328391 31.117 376 252 4 173 543 295 668 6.83e-60 210
MsaG029770 MtrunA17_Chr4g0057891 32.292 384 238 6 174 544 146 520 1.03e-59 206
MsaG029770 MtrunA17_Chr4g0031701 29.904 418 282 4 129 541 212 623 1.22e-59 208
MsaG029770 MtrunA17_Chr2g0328411 31.095 402 270 4 143 543 258 653 3.03e-59 208
MsaG029770 MtrunA17_Chr4g0031731 31.903 373 249 3 173 540 250 622 3.25e-59 207
MsaG029770 MtrunA17_Chr5g0396491 29.792 433 235 7 174 544 53 478 4.64e-58 201
MsaG029770 MtrunA17_Chr2g0328431 32.976 373 244 5 173 543 285 653 5.37e-58 204
MsaG029770 MtrunA17_Chr7g0245601 34.511 368 220 7 181 544 442 792 1.06e-57 206
MsaG029770 MtrunA17_Chr4g0057291 32.461 382 237 10 174 543 289 661 7.30e-52 187
MsaG029770 MtrunA17_Chr5g0401131 29.592 392 253 8 169 544 106 490 7.34e-52 184
MsaG029770 MtrunA17_Chr2g0328401 31.686 344 231 3 173 515 286 626 8.38e-52 187
MsaG029770 MtrunA17_Chr3g0085361 32.749 342 170 7 254 544 18 350 6.64e-50 175
MsaG029770 MtrunA17_Chr1g0200401 31.778 450 254 16 127 544 31 459 1.50e-49 177
MsaG029770 MtrunA17_Chr2g0328361 29.144 374 233 5 173 543 270 614 2.25e-48 177
MsaG029770 MtrunA17_Chr4g0038761 32.791 369 229 8 181 544 88 442 2.66e-47 171
MsaG029770 MtrunA17_Chr4g0039661 32.979 376 237 8 174 544 190 555 5.70e-47 172
MsaG029770 MtrunA17_Chr5g0424131 31.008 387 244 10 174 543 118 498 5.01e-44 163
MsaG029770 MtrunA17_Chr3g0114841 29.426 401 250 10 167 543 108 499 3.11e-43 161
MsaG029770 MtrunA17_Chr2g0328371 32.245 245 159 3 258 500 12 251 2.92e-41 149
MsaG029770 MtrunA17_Chr2g0329591 28.974 390 243 9 177 544 74 451 2.86e-40 151
MsaG029770 MtrunA17_Chr1g0183641 30.053 376 240 10 173 543 119 476 7.71e-40 151
MsaG029770 MtrunA17_Chr2g0294461 25.964 389 260 10 174 544 207 585 8.36e-36 141
MsaG029770 MtrunA17_Chr4g0053901 26.794 418 252 9 174 543 56 467 2.28e-34 135
MsaG029770 MtrunA17_Chr5g0401421 25.806 434 238 12 174 543 32 445 2.51e-34 135
MsaG029770 MtrunA17_Chr2g0294471 24.074 378 270 7 178 544 205 576 1.78e-30 125
MsaG029770 MtrunA17_Chr4g0012071 24.595 370 261 8 178 544 197 551 1.15e-29 122
MsaG029770 MtrunA17_Chr7g0230771 23.944 355 253 7 193 544 391 731 5.57e-27 115
MsaG029770 MtrunA17_Chr7g0230731 23.662 355 254 7 193 544 388 728 9.85e-27 114
MsaG029770 MtrunA17_Chr7g0270031 23.770 366 248 10 190 544 209 554 5.78e-26 111
MsaG029770 MtrunA17_Chr8g0383981 28.186 408 249 19 154 543 114 495 8.42e-26 110
MsaG029770 MtrunA17_Chr1g0194621 23.143 350 246 10 208 544 179 518 1.90e-20 94.7
MsaG029770 MtrunA17_Chr8g0344101 25.679 405 241 17 175 544 221 600 6.52e-19 90.1
MsaG029770 MtrunA17_Chr2g0323111 25.862 174 127 2 371 543 133 305 3.70e-15 76.6
MsaG029770 MtrunA17_Chr4g0054071 33.333 111 71 1 175 282 131 241 1.06e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029770 AT5G48150.1 65.677 539 130 7 12 544 1 490 0.0 702
MsaG029770 AT5G48150.4 65.677 539 130 7 12 544 1 490 0.0 702
MsaG029770 AT5G48150.2 65.677 539 130 7 12 544 1 490 0.0 702
MsaG029770 AT5G48150.3 65.677 539 130 7 12 544 1 490 0.0 702
MsaG029770 AT1G50600.1 61.943 494 180 4 56 544 107 597 0.0 619
MsaG029770 AT1G50600.2 61.943 494 180 4 56 544 111 601 0.0 619
MsaG029770 AT1G50600.3 61.943 494 180 4 56 544 36 526 0.0 617
MsaG029770 AT2G04890.1 68.599 414 123 1 131 544 7 413 0.0 596
MsaG029770 AT4G17230.1 53.016 547 232 9 1 544 1 525 0.0 559
MsaG029770 AT4G17230.2 53.016 547 232 9 1 544 1 525 0.0 559
MsaG029770 AT1G21450.1 48.649 444 211 4 118 544 150 593 1.03e-150 445
MsaG029770 AT3G03450.1 36.800 375 224 7 174 544 180 545 1.51e-70 236
MsaG029770 AT1G66350.1 35.678 398 221 11 158 544 133 506 1.53e-70 235
MsaG029770 AT1G14920.1 36.061 391 225 9 163 544 155 529 3.28e-70 234
MsaG029770 AT2G01570.1 36.869 396 221 12 160 544 204 581 1.60e-68 231
MsaG029770 AT1G07530.1 33.333 375 248 2 171 543 390 764 3.38e-68 234
MsaG029770 AT5G52510.1 34.286 385 228 7 175 544 266 640 2.69e-66 227
MsaG029770 AT5G17490.1 34.933 375 225 9 174 544 157 516 1.52e-65 222
MsaG029770 AT2G29060.2 32.533 375 249 3 173 543 280 654 1.21e-64 223
MsaG029770 AT5G59450.1 31.383 376 254 3 173 544 223 598 1.53e-64 221
MsaG029770 AT2G29060.1 32.095 377 252 2 171 543 315 691 2.07e-64 223
MsaG029770 AT2G37650.2 31.903 373 251 2 173 544 331 701 3.13e-61 214
MsaG029770 AT2G37650.1 31.903 373 251 2 173 544 343 713 3.89e-61 214
MsaG029770 AT3G46600.2 31.452 372 253 2 174 544 1 371 4.61e-61 206
MsaG029770 AT1G50420.1 29.792 433 235 7 174 544 54 479 1.03e-60 208
MsaG029770 AT3G46600.1 31.367 373 254 2 173 544 208 579 4.69e-60 209
MsaG029770 AT3G46600.3 31.367 373 254 2 173 544 176 547 6.97e-60 207
MsaG029770 AT2G29065.1 32.461 382 245 7 173 543 248 627 3.97e-56 199
MsaG029770 AT3G54220.1 34.605 367 222 7 181 543 297 649 4.05e-56 199
MsaG029770 AT1G07520.2 30.446 381 253 5 173 543 150 528 3.63e-55 194
MsaG029770 AT1G07520.4 30.446 381 253 5 173 543 150 528 3.63e-55 194
MsaG029770 AT1G07520.3 30.446 381 253 5 173 543 315 693 1.15e-54 196
MsaG029770 AT1G07520.1 30.446 381 253 5 173 543 314 692 1.25e-54 196
MsaG029770 AT5G66770.1 34.595 370 227 8 181 544 224 584 1.27e-51 186
MsaG029770 AT5G41920.1 32.718 379 231 9 173 544 39 400 2.82e-48 172
MsaG029770 AT3G50650.1 35.294 357 207 9 200 544 198 542 9.61e-48 174
MsaG029770 AT4G37650.1 29.104 402 247 12 170 544 139 529 1.42e-47 174
MsaG029770 AT1G55580.1 30.994 342 204 8 226 544 113 445 1.36e-44 164
MsaG029770 AT1G63100.1 32.665 349 217 6 174 515 249 586 5.55e-44 165
MsaG029770 AT1G63100.2 32.665 349 217 6 174 515 274 611 7.19e-44 165
MsaG029770 AT4G08250.1 28.079 406 270 9 147 543 84 476 2.36e-39 150
MsaG029770 AT3G49950.1 25.389 386 267 9 174 543 27 407 3.25e-31 125
MsaG029770 AT2G45160.1 25.532 376 231 13 191 544 291 639 5.73e-27 115
MsaG029770 AT3G60630.1 28.571 301 190 8 256 544 335 622 9.29e-25 108
MsaG029770 AT3G13840.1 27.749 382 247 12 175 544 146 510 3.41e-24 106
MsaG029770 AT4G36710.1 25.000 380 252 12 178 544 126 485 1.22e-20 95.5
MsaG029770 AT4G00150.1 23.098 368 253 11 182 544 212 554 3.48e-20 94.4
MsaG029770 AT5G67411.1 33.929 112 72 2 370 481 89 198 1.19e-11 64.7

Find 122 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCAGATGGTGTCGGTTTC+TGG 0.116927 5:+81119116 MsaT029770.1:CDS
GTTTGCCCTTACTTCAAATT+TGG 0.195361 5:+81119284 MsaT029770.1:CDS
ATTGGCTTCCTTGAGCAATT+TGG 0.205067 5:-81119376 None:intergenic
CAACAAGTGTCACGACTTTC+GGG 0.226734 5:-81119733 None:intergenic
CATTTCAAGCGAAGCGAAAT+TGG 0.230694 5:-81118970 None:intergenic
CCAATGGATTTCGTTGATTC+AGG 0.238320 5:+81119394 MsaT029770.1:CDS
TCAACAAGTGTCACGACTTT+CGG 0.277772 5:-81119734 None:intergenic
TCCTTACCCTCTGAGTTCTT+TGG 0.281649 5:+81120003 MsaT029770.1:CDS
CAATTTCGCTTCGCTTGAAA+TGG 0.314726 5:+81118971 MsaT029770.1:CDS
CAAGCACAAGCTTAGAGAAT+TGG 0.336532 5:+81118881 MsaT029770.1:CDS
CCACCACACATCCGGATTAC+AGG 0.342966 5:+81119440 MsaT029770.1:CDS
TAGCATGAATGCGAAATTTA+CGG 0.352242 5:-81119636 None:intergenic
GTGTCAGCACTCAGAATCAT+AGG 0.362236 5:+81119678 MsaT029770.1:CDS
ATCATTGTCTGCAATTGCTT+TGG 0.368256 5:-81119060 None:intergenic
TCGATATTGCGGCAAGCAAT+AGG 0.368619 5:-81118580 None:intergenic
AATGGTTGATGGACGAATTA+CGG 0.368703 5:+81119096 MsaT029770.1:CDS
ATCTGGTTGCTTCTTGTGCT+TGG 0.382404 5:+81120122 MsaT029770.1:CDS
TGGTGAACCGATTCAAAGAT+TGG 0.393469 5:+81119136 MsaT029770.1:CDS
CTTGAAGCTACTTAAATCTT+CGG 0.395656 5:-81118862 None:intergenic
CCGGATGTGTGGTGGTCCCC+CGG 0.395807 5:-81119432 None:intergenic
CCTCTGAGTTCTTTGGTGAA+TGG 0.405916 5:+81120010 MsaT029770.1:CDS
GTTGCACGGTTGTCTGCATC+CGG 0.415709 5:+81119182 MsaT029770.1:CDS
TGCAGACAATGATTTGCTAA+TGG 0.419025 5:+81119070 MsaT029770.1:CDS
ATGTAACCAAATTTGAAGTA+AGG 0.431720 5:-81119290 None:intergenic
TTTGGTTACATGTCTGCAAA+TGG 0.433675 5:+81119302 MsaT029770.1:CDS
GTTATGCACGATCCACTCAT+TGG 0.435852 5:-81118840 None:intergenic
GCTAATGGCGCAATGGTTGA+TGG 0.437770 5:+81119085 MsaT029770.1:CDS
TGAGATTCTTGCTGCGACAT+TGG 0.437774 5:-81118768 None:intergenic
TTTCACGCTGCAGCTATCTC+TGG 0.438144 5:+81119563 MsaT029770.1:CDS
AAAGCTTGCTCGGCATTTCA+AGG 0.448893 5:+81119529 MsaT029770.1:CDS
AGATTTGTAGATTGAACTCC+CGG 0.451937 5:-81119201 None:intergenic
GGCGAGCCATAGGTATTGTC+GGG 0.452043 5:-81118819 None:intergenic
ACTCCAACGTGCAGTAATAA+AGG 0.452114 5:-81118674 None:intergenic
CATAACCTTGCAGTTCGGCC+AGG 0.453170 5:+81119602 MsaT029770.1:CDS
GAAATCAATTATATGAACTC+TGG 0.454657 5:-81119354 None:intergenic
TTGGAGTCGTCCTTGGCAGC+TGG 0.456230 5:+81118690 MsaT029770.1:CDS
TCAGCTTATGCCCGTGGAGG+TGG 0.460311 5:+81119479 MsaT029770.1:CDS
ATCCTCTCTTTATGCTCTCT+CGG 0.463692 5:-81119854 None:intergenic
AGGTCAAGATTTGCAATGGC+TGG 0.465717 5:+81119974 MsaT029770.1:CDS
ATCTTTAAGATGCAAAGAAC+CGG 0.467079 5:+81119220 MsaT029770.1:CDS
TGGCGAGCCATAGGTATTGT+CGG 0.470434 5:-81118820 None:intergenic
AGTTCGGCCAGGGGAAGCTC+TGG 0.474090 5:+81119613 MsaT029770.1:CDS
AACTGCATAACCTTGCAGTT+CGG 0.476621 5:+81119597 MsaT029770.1:CDS
TATGCTCCCAATCTTTGAAT+CGG 0.477205 5:-81119143 None:intergenic
AGAGATGGAGCTCTATATCT+TGG 0.480538 5:+81120085 MsaT029770.1:CDS
TAGGCTTCAATTTGCTGCAA+TGG 0.480682 5:-81118561 None:intergenic
TGAGTGCTGACACTCTCATC+TGG 0.480842 5:-81119668 None:intergenic
AGGCCAAGCAACTCAATTCT+CGG 0.481276 5:+81118635 MsaT029770.1:CDS
CAATGTTGAGCAGCATTGCT+TGG 0.481276 5:+81119877 MsaT029770.1:CDS
GGTGAACCGATTCAAAGATT+GGG 0.489961 5:+81119137 MsaT029770.1:CDS
CTGCACGTTGGAGTCGTCCT+TGG 0.491134 5:+81118683 MsaT029770.1:CDS
CTATCAAGATTATCAGAGTC+AGG 0.491387 5:-81118921 None:intergenic
GAGCCGAGAATTGAGTTGCT+TGG 0.498447 5:-81118638 None:intergenic
TAATAAAGGTCAGAAGAAGT+TGG 0.499526 5:-81118660 None:intergenic
CATCAGCTGTACAACAATGA+TGG 0.501721 5:+81118612 MsaT029770.1:CDS
AACAAAATTTCCAGCTGCCA+AGG 0.505944 5:-81118700 None:intergenic
CTTCGCTTGAAATGGACAGT+TGG 0.507924 5:+81118979 MsaT029770.1:CDS
ACCAAAGAACTCAGAGGGTA+AGG 0.509605 5:-81120004 None:intergenic
GATCATCAATACCTGTAATC+CGG 0.510867 5:-81119451 None:intergenic
GACACGAGCTGCTAGGGAAG+TGG 0.514693 5:+81119951 MsaT029770.1:CDS
GAGAGAAGCTCGGCACTCTC+CGG 0.516041 5:-81119239 None:intergenic
GTGTCACGACTTTCGGGGAT+AGG 0.518113 5:-81119727 None:intergenic
TCCACTCATTGGCGAGCCAT+AGG 0.519637 5:-81118829 None:intergenic
TGGAACGACACGAGCTGCTA+GGG 0.521228 5:+81119945 MsaT029770.1:CDS
GTGGAACGACACGAGCTGCT+AGG 0.524981 5:+81119944 MsaT029770.1:CDS
ATGGAGCTCTATATCTTGGT+TGG 0.526117 5:+81120089 MsaT029770.1:CDS
TGGTTGGATGAATAGAGATC+TGG 0.528352 5:+81120105 MsaT029770.1:CDS
TTGCTTTGGCACATGCAGTA+AGG 0.529396 5:-81119046 None:intergenic
ATAACCTTGCAGTTCGGCCA+GGG 0.532226 5:+81119603 MsaT029770.1:CDS
GTTAACATCATAGCTTGTGA+AGG 0.534917 5:+81119911 MsaT029770.1:CDS
CGAATTACGGCAGATGGTGT+CGG 0.542149 5:+81119109 MsaT029770.1:CDS
TGATTTAGATTTCGATATTG+CGG 0.543411 5:-81118591 None:intergenic
GCTGCAGCGTGAAACTCAAA+TGG 0.545283 5:-81119554 None:intergenic
TGTAACCAAATTTGAAGTAA+GGG 0.545514 5:-81119289 None:intergenic
ACCTATGGCTCGCCAATGAG+TGG 0.546193 5:+81118828 MsaT029770.1:CDS
ATTGATTTCCAAATTGCTCA+AGG 0.548833 5:+81119368 MsaT029770.1:CDS
GCACTCAGAATCATAGGGAT+AGG 0.551891 5:+81119684 MsaT029770.1:CDS
TTAACATCATAGCTTGTGAA+GGG 0.552742 5:+81119912 MsaT029770.1:CDS
CCTGAATCAACGAAATCCAT+TGG 0.554910 5:-81119394 None:intergenic
GGGGAATGATACTTATCAGA+TGG 0.562828 5:-81118798 None:intergenic
TGCGACATTGGACTATCGTT+AGG 0.563587 5:-81118756 None:intergenic
TTTACGGCCAGAGCTTCCCC+TGG 0.565827 5:-81119620 None:intergenic
GAATTGGAAAATGTAATGCT+TGG 0.570289 5:+81118897 MsaT029770.1:CDS
TTGCACGGTTGTCTGCATCC+GGG 0.585418 5:+81119183 MsaT029770.1:CDS
TGTTGGAAGGACTCGTTGCA+CGG 0.585796 5:+81119168 MsaT029770.1:CDS
TATATGTAGTCCACCTCCAC+GGG 0.589655 5:-81119489 None:intergenic
TATAGACTTGAAGAGAGAGA+TGG 0.590094 5:+81120070 MsaT029770.1:CDS
CTTCCCCTGGCCGAACTGCA+AGG 0.590913 5:-81119607 None:intergenic
TTGTGAAGGGGTCGAGAGAG+TGG 0.594827 5:+81119925 MsaT029770.1:CDS
GACTACATATAGTTGAAAAG+AGG 0.595404 5:+81119501 MsaT029770.1:CDS
TGTCAGCACTCAGAATCATA+GGG 0.595636 5:+81119679 MsaT029770.1:CDS
AGAGGTTATCAAAGCTTGCT+CGG 0.596778 5:+81119519 MsaT029770.1:CDS
AAGATTGGGAGCATACATGT+TGG 0.605912 5:+81119151 MsaT029770.1:CDS
CTATATGTAGTCCACCTCCA+CGG 0.608482 5:-81119490 None:intergenic
TCCATAGTTTCGAGAAAACG+TGG 0.611195 5:-81119791 None:intergenic
GTGGAGGTCAAGATTTGCAA+TGG 0.615832 5:+81119970 MsaT029770.1:CDS
TTGGGAGCATACATGTTGGA+AGG 0.617711 5:+81119155 MsaT029770.1:CDS
TACGACAGTGCTATCAGCAA+CGG 0.617830 5:+81118945 MsaT029770.1:CDS
TGACCTTTATTACTGCACGT+TGG 0.620270 5:+81118671 MsaT029770.1:CDS
GGCGAGAAACTAACAGTCGA+AGG 0.623572 5:-81118735 None:intergenic
AAATTGCTCAAGGAAGCCAA+TGG 0.626215 5:+81119378 MsaT029770.1:CDS
GATGGACGAATTACGGCAGA+TGG 0.627072 5:+81119103 MsaT029770.1:CDS
TTACATGAACATACTTTACG+AGG 0.627108 5:+81119262 MsaT029770.1:CDS
TAACCTTGCAGTTCGGCCAG+GGG 0.630683 5:+81119604 MsaT029770.1:CDS
ACGAGCTGCTAGGGAAGTGG+AGG 0.634312 5:+81119954 MsaT029770.1:CDS
CCACGGGCATAAGCTGATGT+CGG 0.634703 5:-81119473 None:intergenic
CATTCACCAAAGAACTCAGA+GGG 0.637813 5:-81120009 None:intergenic
CCGACATCAGCTTATGCCCG+TGG 0.638695 5:+81119473 MsaT029770.1:CDS
ATGATTTGCTAATGGCGCAA+TGG 0.646542 5:+81119078 MsaT029770.1:CDS
TTCCGAGAGAGCATAAAGAG+AGG 0.651166 5:+81119852 MsaT029770.1:CDS
GCGAGCCATAGGTATTGTCG+GGG 0.657718 5:-81118818 None:intergenic
CAGCTGTACAACAATGATGG+AGG 0.659045 5:+81118615 MsaT029770.1:CDS
ACATCAGCTTATGCCCGTGG+AGG 0.663564 5:+81119476 MsaT029770.1:CDS
TAACATCATAGCTTGTGAAG+GGG 0.668817 5:+81119913 MsaT029770.1:CDS
CCTGTAATCCGGATGTGTGG+TGG 0.675147 5:-81119440 None:intergenic
GGACAGTTGGAGACAAACAA+TGG 0.681512 5:+81118992 MsaT029770.1:CDS
AACAAGTGTCACGACTTTCG+GGG 0.682943 5:-81119732 None:intergenic
GTTCATGTAAGAGAGAAGCT+CGG 0.686963 5:-81119249 None:intergenic
CCATTCACCAAAGAACTCAG+AGG 0.710837 5:-81120010 None:intergenic
ATACCTGTAATCCGGATGTG+TGG 0.719840 5:-81119443 None:intergenic
CGAGCCATAGGTATTGTCGG+GGG 0.727707 5:-81118817 None:intergenic
AGAATCTAACACAAACACTG+CGG 0.784454 5:+81119760 MsaT029770.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 81118516 81120150 81118516 ID=MsaG029770
Chr5 mRNA 81118516 81120150 81118516 ID=MsaT029770.1;Parent=MsaG029770
Chr5 exon 81118516 81120150 81118516 ID=MsaT029770.1.exon1;Parent=MsaT029770.1
Chr5 CDS 81118516 81120150 81118516 ID=cds.MsaT029770.1;Parent=MsaT029770.1
Gene Sequence

>MsaT029770.1

ATGCAAGCATCAGAGCAGCATAGAAGTTCAAGTATGTACTATCAACCATTGCAGCAAATTGAAGCCTATTGCTTGCCGCAATATCGAAATCTAAATCATCAGCTGTACAACAATGATGGAGGCCAAGCAACTCAATTCTCGGCTCCAACTTCTTCTGACCTTTATTACTGCACGTTGGAGTCGTCCTTGGCAGCTGGAAATTTTGTTGTTTACAACTCTCCTTCGACTGTTAGTTTCTCGCCTAACGATAGTCCAATGTCGCAGCAAGAATCTCAGTCTTATCCATCTGATAAGTATCATTCCCCCGACAATACCTATGGCTCGCCAATGAGTGGATCGTGCATAACCGAAGATTTAAGTAGCTTCAAGCACAAGCTTAGAGAATTGGAAAATGTAATGCTTGGTCCTGACTCTGATAATCTTGATAGTTACGACAGTGCTATCAGCAACGGAACCAATTTCGCTTCGCTTGAAATGGACAGTTGGAGACAAACAATGGTAGCAATTTCGAGCAAAAATCTAAAGCACATCCTTACTGCATGTGCCAAAGCAATTGCAGACAATGATTTGCTAATGGCGCAATGGTTGATGGACGAATTACGGCAGATGGTGTCGGTTTCTGGTGAACCGATTCAAAGATTGGGAGCATACATGTTGGAAGGACTCGTTGCACGGTTGTCTGCATCCGGGAGTTCAATCTACAAATCTTTAAGATGCAAAGAACCGGAGAGTGCCGAGCTTCTCTCTTACATGAACATACTTTACGAGGTTTGCCCTTACTTCAAATTTGGTTACATGTCTGCAAATGGAGCCATTGCAGAAGCCATGAAAAATGAAGCCAGAGTTCATATAATTGATTTCCAAATTGCTCAAGGAAGCCAATGGATTTCGTTGATTCAGGCTTTTGCAGCTAGGCCCGGGGGACCACCACACATCCGGATTACAGGTATTGATGATCCGACATCAGCTTATGCCCGTGGAGGTGGACTACATATAGTTGAAAAGAGGTTATCAAAGCTTGCTCGGCATTTCAAGGTGCCATTTGAGTTTCACGCTGCAGCTATCTCTGGTTGTGATGTTCAACTGCATAACCTTGCAGTTCGGCCAGGGGAAGCTCTGGCCGTAAATTTCGCATTCATGCTACATCACATGCCAGATGAGAGTGTCAGCACTCAGAATCATAGGGATAGGCTTTTAAGGTTGGTTAAGAGCCTATCCCCGAAAGTCGTGACACTTGTTGAGCAAGAATCTAACACAAACACTGCGGCATTCTTTCCACGTTTTCTCGAAACTATGGACTATTACACAGCAATGTTCGAGTCAATAGACGTGACTCTTCCGAGAGAGCATAAAGAGAGGATCAATGTTGAGCAGCATTGCTTGGCAAGAGATTTAGTTAACATCATAGCTTGTGAAGGGGTCGAGAGAGTGGAACGACACGAGCTGCTAGGGAAGTGGAGGTCAAGATTTGCAATGGCTGGTTTCACTCCTTACCCTCTGAGTTCTTTGGTGAATGGTACCATAAAGAAATTGCTTGAAAACTACAGTGATAGATATAGACTTGAAGAGAGAGATGGAGCTCTATATCTTGGTTGGATGAATAGAGATCTGGTTGCTTCTTGTGCTTGGAAATGA
Protein sequence

>MsaT029770.1

MQASEQHRSSSMYYQPLQQIEAYCLPQYRNLNHQLYNNDGGQATQFSAPTSSDLYYCTLESSLAAGNFVVYNSPSTVSFSPNDSPMSQQESQSYPSDKYHSPDNTYGSPMSGSCITEDLSSFKHKLRELENVMLGPDSDNLDSYDSAISNGTNFASLEMDSWRQTMVAISSKNLKHILTACAKAIADNDLLMAQWLMDELRQMVSVSGEPIQRLGAYMLEGLVARLSASGSSIYKSLRCKEPESAELLSYMNILYEVCPYFKFGYMSANGAIAEAMKNEARVHIIDFQIAQGSQWISLIQAFAARPGGPPHIRITGIDDPTSAYARGGGLHIVEKRLSKLARHFKVPFEFHAAAISGCDVQLHNLAVRPGEALAVNFAFMLHHMPDESVSTQNHRDRLLRLVKSLSPKVVTLVEQESNTNTAAFFPRFLETMDYYTAMFESIDVTLPREHKERINVEQHCLARDLVNIIACEGVERVERHELLGKWRSRFAMAGFTPYPLSSLVNGTIKKLLENYSDRYRLEERDGALYLGWMNRDLVASCAWK*