AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029786

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029786 MtrunA17_Chr7g0214071 88.710 62 7 0 1 62 4 65 4.79e-36 124
MsaG029786 MtrunA17_Chr7g0214071 59.140 93 15 4 60 129 164 256 1.33e-13 65.1
MsaG029786 MtrunA17_Chr8g0385991 49.558 113 48 2 1 113 4 107 3.48e-27 100
MsaG029786 MtrunA17_Chr3g0131911 47.826 92 47 1 1 92 4 94 3.45e-23 90.5
MsaG029786 MtrunA17_Chr7g0263931 44.318 88 38 1 7 83 6 93 1.70e-19 82.0
MsaG029786 MtrunA17_Chr2g0318101 65.455 55 19 0 12 66 15 69 1.41e-18 79.7
MsaG029786 MtrunA17_Chr7g0252971 56.897 58 25 0 9 66 5 62 1.48e-18 79.7
MsaG029786 MtrunA17_Chr4g0047841 62.500 56 21 0 11 66 14 69 6.40e-18 77.8
MsaG029786 MtrunA17_Chr3g0124761 58.621 58 24 0 9 66 4 61 1.97e-17 75.9
MsaG029786 MtrunA17_Chr5g0417791 38.532 109 46 1 10 97 1 109 2.95e-17 75.1
MsaG029786 MtrunA17_Chr2g0326361 60.000 55 22 0 12 66 14 68 6.03e-17 75.1
MsaG029786 MtrunA17_Chr4g0042561 38.318 107 45 1 12 97 9 115 1.50e-16 73.6
MsaG029786 MtrunA17_Chr3g0109821 64.000 50 18 0 10 59 4 53 1.17e-15 72.0
MsaG029786 MtrunA17_Chr5g0417001 45.745 94 40 3 5 94 4 90 3.26e-15 70.5
MsaG029786 MtrunA17_Chr3g0091921 33.333 150 76 2 3 128 7 156 3.93e-15 69.7
MsaG029786 MtrunA17_Chr8g0361731 46.512 86 39 2 9 94 12 90 7.14e-15 69.7
MsaG029786 MtrunA17_Chr4g0060931 34.459 148 73 2 5 128 10 157 8.03e-15 69.3
MsaG029786 MtrunA17_Chr5g0433781 36.937 111 47 2 10 97 4 114 1.20e-14 69.3
MsaG029786 MtrunA17_Chr6g0453131 58.182 55 23 0 12 66 15 69 3.05e-14 67.8
MsaG029786 MtrunA17_Chr8g0384721 36.036 111 51 2 7 97 2 112 5.64e-14 66.6
MsaG029786 MtrunA17_Chr2g0311661 53.571 56 25 1 3 58 13 67 6.86e-14 66.2
MsaG029786 MtrunA17_Chr6g0484441 51.724 58 28 0 9 66 2 59 8.52e-14 66.2
MsaG029786 MtrunA17_Chr6g0453451 32.174 115 57 1 4 97 12 126 1.07e-13 65.9
MsaG029786 MtrunA17_Chr3g0113011 50.943 53 26 0 7 59 14 66 1.30e-13 61.6
MsaG029786 MtrunA17_Chr2g0316971 51.724 58 28 0 9 66 20 77 1.46e-13 65.9
MsaG029786 MtrunA17_Chr5g0442771 53.226 62 28 1 5 66 12 72 1.75e-13 65.5
MsaG029786 MtrunA17_Chr2g0311651 54.545 55 24 1 4 58 15 68 1.79e-13 65.5
MsaG029786 MtrunA17_Chr4g0018391 33.333 111 50 1 11 97 6 116 2.21e-13 65.1
MsaG029786 MtrunA17_Chr3g0139331 51.724 58 28 0 9 66 8 65 2.80e-13 65.1
MsaG029786 MtrunA17_Chr2g0309891 57.143 49 21 0 10 58 4 52 3.79e-13 64.7
MsaG029786 MtrunA17_Chr7g0253011 49.254 67 31 1 3 66 5 71 4.87e-13 64.3
MsaG029786 MtrunA17_Chr4g0054791 59.574 47 19 0 12 58 6 52 4.89e-13 64.3
MsaG029786 MtrunA17_Chr8g0359921 53.448 58 27 0 9 66 11 68 6.42e-13 63.9
MsaG029786 MtrunA17_Chr6g0481091 60.417 48 19 0 10 57 4 51 8.49e-13 62.8
MsaG029786 MtrunA17_Chr2g0318081 50.847 59 29 0 8 66 12 70 9.03e-13 63.5
MsaG029786 MtrunA17_Chr5g0400461 54.902 51 23 0 8 58 2 52 9.35e-13 63.5
MsaG029786 MtrunA17_Chr2g0309331 43.548 62 35 0 3 64 1 62 1.18e-12 63.2
MsaG029786 MtrunA17_Chr7g0217711 52.542 59 28 0 7 65 14 72 1.31e-12 61.2
MsaG029786 MtrunA17_Chr5g0405211 30.909 110 52 1 12 97 8 117 2.24e-12 62.4
MsaG029786 MtrunA17_Chr5g0398971 53.704 54 25 0 11 64 6 59 2.45e-12 62.4
MsaG029786 MtrunA17_Chr4g0056541 33.333 111 50 1 11 97 6 116 4.28e-12 61.6
MsaG029786 MtrunA17_Chr3g0130731 53.846 52 24 0 7 58 2 53 5.40e-12 61.2
MsaG029786 MtrunA17_Chr3g0113041 49.057 53 27 0 7 59 14 66 8.12e-12 57.0
MsaG029786 MtrunA17_Chr1g0197351 60.870 46 18 0 12 57 42 87 9.82e-12 60.8
MsaG029786 MtrunA17_Chr7g0217721 50.847 59 29 0 7 65 14 72 1.04e-11 60.5
MsaG029786 MtrunA17_Chr1g0147421 37.097 124 51 5 9 105 24 147 1.13e-11 60.5
MsaG029786 MtrunA17_Chr8g0372121 30.000 110 53 1 12 97 6 115 1.14e-11 60.5
MsaG029786 MtrunA17_Chr1g0200561 30.909 110 52 1 12 97 19 128 2.36e-11 59.7
MsaG029786 MtrunA17_Chr2g0312321 32.773 119 51 3 9 103 8 121 2.67e-11 59.3
MsaG029786 MtrunA17_Chr8g0345671 53.061 49 22 1 12 59 11 59 4.14e-11 58.9
MsaG029786 MtrunA17_Chr5g0428991 54.167 48 22 0 11 58 24 71 5.30e-11 58.5
MsaG029786 MtrunA17_Chr4g0050701 54.167 48 22 0 12 59 11 58 5.73e-11 57.4
MsaG029786 MtrunA17_Chr4g0017921 52.830 53 25 0 12 64 8 60 6.72e-11 58.2
MsaG029786 MtrunA17_Chr6g0461661 45.614 57 31 0 10 66 16 72 6.73e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029786 AT2G33480.2 47.321 112 41 1 4 97 7 118 2.04e-27 102
MsaG029786 AT2G33480.1 47.321 112 41 1 4 97 7 118 2.10e-27 102
MsaG029786 AT5G13180.1 75.000 56 14 0 7 62 9 64 9.02e-26 97.8
MsaG029786 AT1G61110.1 49.398 83 42 0 8 90 12 94 1.26e-18 80.1
MsaG029786 AT3G04070.1 63.636 55 20 0 12 66 10 64 3.90e-18 79.0
MsaG029786 AT3G04070.2 63.636 55 20 0 12 66 10 64 4.60e-18 78.6
MsaG029786 AT5G39610.1 37.288 118 53 1 1 97 9 126 1.24e-17 77.0
MsaG029786 AT1G69490.1 42.056 107 41 3 12 97 9 115 4.93e-17 75.1
MsaG029786 AT5G14000.2 45.455 77 42 0 7 83 10 86 5.51e-17 72.8
MsaG029786 AT1G77450.1 51.562 64 31 0 3 66 1 64 7.84e-17 74.3
MsaG029786 AT5G14000.1 45.455 77 42 0 7 83 10 86 2.70e-16 72.0
MsaG029786 AT3G04060.1 57.895 57 24 0 10 66 18 74 4.47e-16 73.2
MsaG029786 AT1G65910.1 60.000 50 20 0 10 59 4 53 4.96e-16 73.6
MsaG029786 AT5G61430.1 32.000 150 78 2 3 128 7 156 7.58e-16 72.4
MsaG029786 AT3G15510.1 64.706 51 18 0 16 66 21 71 8.78e-16 72.4
MsaG029786 AT3G29035.1 52.381 63 30 0 4 66 16 78 1.01e-15 72.0
MsaG029786 AT5G14000.3 56.604 53 23 0 7 59 10 62 1.35e-15 71.2
MsaG029786 AT1G52880.1 62.745 51 19 0 16 66 21 71 3.01e-15 70.9
MsaG029786 AT3G03200.1 58.000 50 21 0 10 59 4 53 5.65e-15 70.5
MsaG029786 AT1G33060.1 56.250 48 21 0 12 59 24 71 9.37e-15 69.7
MsaG029786 AT1G33060.2 56.250 48 21 0 12 59 24 71 9.65e-15 69.7
MsaG029786 AT5G07680.1 30.405 148 79 2 5 128 10 157 1.02e-14 69.3
MsaG029786 AT5G39820.1 55.385 65 28 1 2 66 11 74 1.24e-14 68.9
MsaG029786 AT5G46590.1 34.286 140 65 3 8 120 2 141 1.52e-14 68.6
MsaG029786 AT5G46590.2 34.286 140 65 3 8 120 2 141 1.60e-14 68.6
MsaG029786 AT5G07680.2 31.915 141 72 2 12 128 3 143 1.89e-14 68.6
MsaG029786 AT3G17730.1 31.944 144 74 2 10 129 4 147 2.61e-14 67.4
MsaG029786 AT5G17260.1 62.000 50 19 0 10 59 4 53 3.36e-14 68.2
MsaG029786 AT1G01720.1 54.386 57 26 0 10 66 5 61 4.75e-14 67.0
MsaG029786 AT1G26870.1 45.349 86 40 2 9 94 14 92 5.43e-14 67.4
MsaG029786 AT5G53950.1 54.545 55 25 0 12 66 17 71 6.08e-14 67.4
MsaG029786 AT5G63790.1 50.000 64 32 0 3 66 41 104 8.33e-14 66.6
MsaG029786 AT3G15170.1 53.448 58 27 0 9 66 17 74 8.48e-14 66.6
MsaG029786 AT5G63790.2 50.000 64 32 0 3 66 51 114 8.60e-14 66.6
MsaG029786 AT4G27410.2 51.724 58 28 0 9 66 11 68 9.57e-14 66.2
MsaG029786 AT1G54330.1 63.830 47 17 0 12 58 6 52 1.02e-13 66.2
MsaG029786 AT5G04395.1 57.143 49 21 0 9 57 25 73 1.56e-13 64.3
MsaG029786 AT5G18270.2 52.632 57 27 0 10 66 19 75 1.60e-13 65.9
MsaG029786 AT4G17980.2 34.483 116 51 2 8 98 2 117 1.71e-13 65.5
MsaG029786 AT4G27410.3 56.000 50 22 0 9 58 11 60 1.91e-13 65.9
MsaG029786 AT4G17980.1 34.483 116 51 2 8 98 2 117 2.04e-13 65.1
MsaG029786 AT5G18270.1 52.632 57 27 0 10 66 19 75 2.18e-13 65.5
MsaG029786 AT5G64530.2 56.000 50 22 0 10 59 1 50 5.51e-13 62.4
MsaG029786 AT5G64530.1 56.000 50 22 0 10 59 1 50 8.12e-13 62.4
MsaG029786 AT3G18400.1 50.000 58 29 0 9 66 2 59 1.06e-12 63.5
MsaG029786 AT1G76420.1 51.724 58 28 0 9 66 19 76 1.12e-12 63.5
MsaG029786 AT4G35580.3 54.167 48 22 0 12 59 9 56 1.24e-12 63.9
MsaG029786 AT4G35580.2 54.167 48 22 0 12 59 9 56 1.24e-12 63.9
MsaG029786 AT4G35580.1 54.167 48 22 0 12 59 9 56 1.60e-12 63.5
MsaG029786 AT5G66300.1 31.405 121 59 1 1 97 1 121 1.84e-12 62.8
MsaG029786 AT1G52890.1 48.276 58 30 0 9 66 11 68 1.85e-12 62.8
MsaG029786 AT3G61910.1 44.828 58 32 0 7 64 6 63 2.19e-12 62.8
MsaG029786 AT5G08790.1 53.448 58 27 0 9 66 4 61 2.31e-12 62.4
MsaG029786 AT4G36160.2 31.818 110 51 1 12 97 14 123 3.48e-12 62.4
MsaG029786 AT4G36160.1 31.818 110 51 1 12 97 10 119 3.70e-12 62.4
MsaG029786 AT4G36160.3 31.818 110 51 1 12 97 10 119 3.70e-12 62.4
MsaG029786 AT2G18060.3 30.909 110 52 1 12 97 9 118 4.33e-12 62.0
MsaG029786 AT2G18060.1 30.909 110 52 1 12 97 9 118 4.33e-12 62.0
MsaG029786 AT2G46770.1 48.276 58 30 0 7 64 11 68 5.53e-12 61.6
MsaG029786 AT1G12260.2 51.786 56 27 0 11 66 6 61 6.54e-12 61.6
MsaG029786 AT1G32770.1 29.932 147 76 2 7 129 11 154 8.39e-12 61.2
MsaG029786 AT1G79580.3 45.902 61 33 0 4 64 9 69 1.02e-11 60.8
MsaG029786 AT1G79580.5 45.902 61 33 0 4 64 9 69 1.02e-11 60.8
MsaG029786 AT1G79580.4 45.902 61 33 0 4 64 9 69 1.02e-11 60.8
MsaG029786 AT1G79580.2 45.902 61 33 0 4 64 9 69 1.02e-11 60.8
MsaG029786 AT1G79580.1 45.902 61 33 0 4 64 9 69 1.02e-11 60.8
MsaG029786 AT5G24590.2 53.191 47 22 0 12 58 13 59 1.35e-11 60.8
MsaG029786 AT5G50820.1 40.964 83 40 2 2 76 7 88 1.42e-11 59.3
MsaG029786 AT5G62380.2 32.743 113 50 3 10 97 5 116 1.80e-11 60.5
MsaG029786 AT5G62380.1 32.743 113 50 3 10 97 5 116 1.80e-11 60.5
MsaG029786 AT2G24430.2 36.607 112 48 3 9 97 13 124 2.43e-11 59.7
MsaG029786 AT2G24430.1 36.607 112 48 3 9 97 13 124 2.43e-11 59.7
MsaG029786 AT3G10490.1 58.696 46 19 0 12 57 27 72 2.95e-11 58.9
MsaG029786 AT5G04410.1 59.574 47 19 0 12 58 9 55 3.23e-11 59.7
MsaG029786 AT2G43000.1 56.000 50 21 1 9 58 16 64 3.71e-11 58.9
MsaG029786 AT3G15500.1 46.552 58 31 0 9 66 11 68 4.23e-11 59.3
MsaG029786 AT3G10500.1 57.447 47 20 0 12 58 9 55 4.30e-11 59.3
MsaG029786 AT1G71930.2 29.091 110 54 1 12 97 9 118 8.49e-11 58.2
MsaG029786 AT1G71930.1 29.091 110 54 1 12 97 9 118 8.49e-11 58.2

Find 29 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCACTCCCATCCTCTTT+TGG 0.255864 5:+81270323 MsaT029786.1:CDS
ATCTTTGCAAGTCTGATCCT+TGG 0.370348 5:+81269542 MsaT029786.1:CDS
TCTTTGCAAGTCTGATCCTT+GGG 0.393386 5:+81269543 MsaT029786.1:CDS
GAATGATGGAAGCGGGTAAA+GGG 0.423136 5:-81269510 None:intergenic
CTTCATCTGTTGGATGAAAA+CGG 0.429980 5:-81269447 None:intergenic
CCAATGTTGCTGCCACTGCT+TGG 0.457753 5:-81270182 None:intergenic
ACAACAAGTTCTTCATCTGT+TGG 0.464543 5:-81269457 None:intergenic
AAGATCAACTTGAGGAATGA+TGG 0.468508 5:-81269524 None:intergenic
TTCATTTGTGATTGAGGAGG+AGG 0.474326 5:-81270291 None:intergenic
CCAAGCAGTGGCAGCAACAT+TGG 0.482032 5:+81270182 MsaT029786.1:CDS
GGAATGATGGAAGCGGGTAA+AGG 0.497273 5:-81269511 None:intergenic
TGTTCAATATTTGAAGCCTA+AGG 0.506309 5:+81269477 MsaT029786.1:CDS
CTCAGCTTCGAGTTCAAAAG+TGG 0.508234 5:+81270223 MsaT029786.1:CDS
GCTGTCTTCATTTGTGATTG+AGG 0.509175 5:-81270297 None:intergenic
TAAGCATTTCCAAAAGAGGA+TGG 0.518802 5:-81270332 None:intergenic
GGATTAAGCATTTCCAAAAG+AGG 0.521576 5:-81270336 None:intergenic
AAAGGGAAAGAGAAGACCTT+AGG 0.551985 5:-81269493 None:intergenic
AAGCATTTCCAAAAGAGGAT+GGG 0.563423 5:-81270331 None:intergenic
AATGGAGAAGAGACTGTGAA+AGG 0.563453 5:+81270152 MsaT029786.1:CDS
TCTGATCCTTGGGATTTACC+TGG 0.570788 5:+81269553 MsaT029786.1:CDS
GGATGAAAACGGAAACCTGC+TGG 0.571341 5:-81269436 None:intergenic
GTCTTCATTTGTGATTGAGG+AGG 0.581319 5:-81270294 None:intergenic
ACTTGAGGAATGATGGAAGC+GGG 0.582789 5:-81269517 None:intergenic
AAAGGAGTGAAGCCAAGCAG+TGG 0.603258 5:+81270170 MsaT029786.1:CDS
GGTGAAGTGAGATTGCCAGC+AGG 0.604676 5:+81269421 MsaT029786.1:CDS
TACATACCAGGTAAATCCCA+AGG 0.614952 5:-81269559 None:intergenic
AACTTGAGGAATGATGGAAG+CGG 0.644118 5:-81269518 None:intergenic
CAGTGGCAGCAACATTGGTG+AGG 0.667609 5:+81270187 MsaT029786.1:CDS
GACTTGCAAAGATCAACTTG+AGG 0.673094 5:-81269532 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 81269400 81270355 81269400 ID=MsaG029786
Chr5 mRNA 81269400 81270355 81269400 ID=MsaT029786.1;Parent=MsaG029786
Chr5 exon 81269400 81269574 81269400 ID=MsaT029786.1.exon1;Parent=MsaT029786.1
Chr5 CDS 81269400 81269574 81269400 ID=cds.MsaT029786.1;Parent=MsaT029786.1
Chr5 exon 81270141 81270355 81270141 ID=MsaT029786.1.exon2;Parent=MsaT029786.1
Chr5 CDS 81270141 81270355 81270141 ID=cds.MsaT029786.1;Parent=MsaT029786.1
Gene Sequence

>MsaT029786.1

ATGAATTTTGTGAAGAATAATGGTGAAGTGAGATTGCCAGCAGGTTTCCGTTTTCATCCAACAGATGAAGAACTTGTTGTTCAATATTTGAAGCCTAAGGTCTTCTCTTTCCCTTTACCCGCTTCCATCATTCCTCAAGTTGATCTTTGCAAGTCTGATCCTTGGGATTTACCTGGTGGTGTTAAAAATGGAGAAGAGACTGTGAAAGGAGTGAAGCCAAGCAGTGGCAGCAACATTGGTGAGGTGAAGAAGAAGAACTCAGCTTCGAGTTCAAAAGTGGTTGTTTTCTATGATTTCTTCGCAGAGAAGAGCAATACTGATGCATCCTCCTCCTCAATCACAAATGAAGACAGCAGTAACTCACTCCCATCCTCTTTTGGAAATGCTTAA
Protein sequence

>MsaT029786.1

MNFVKNNGEVRLPAGFRFHPTDEELVVQYLKPKVFSFPLPASIIPQVDLCKSDPWDLPGGVKNGEETVKGVKPSSGSNIGEVKKKNSASSSKVVVFYDFFAEKSNTDASSSSITNEDSSNSLPSSFGNA*