AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029810


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029810 MtrunA17_Chr5g0447971 76.672 583 71 4 7 588 14 532 0.0 834
MsaG029810 MtrunA17_Chr3g0086321 68.240 233 70 1 360 588 161 393 1.13e-100 311
MsaG029810 MtrunA17_Chr3g0086321 76.613 124 25 1 65 188 80 199 2.51e-56 194
MsaG029810 MtrunA17_Chr3g0086321 32.766 235 110 6 296 490 161 387 1.63e-17 85.1
MsaG029810 MtrunA17_Chr3g0086321 30.120 166 92 3 417 580 82 225 3.35e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029810 AT5G61190.10 27.128 188 106 5 393 580 240 396 1.78e-11 67.8
MsaG029810 AT5G61190.4 26.702 191 109 5 393 583 240 399 2.06e-11 67.4
MsaG029810 AT5G61190.2 26.702 191 109 5 393 583 240 399 2.06e-11 67.4
MsaG029810 AT5G61190.1 27.128 188 106 5 393 580 240 396 2.07e-11 67.8
MsaG029810 AT5G61190.7 27.128 188 106 5 393 580 146 302 2.17e-11 67.4
MsaG029810 AT5G61190.3 27.128 188 106 5 393 580 146 302 2.17e-11 67.4
MsaG029810 AT5G61190.11 27.128 188 106 5 393 580 240 396 2.78e-11 67.0
MsaG029810 AT5G61190.8 27.128 188 106 5 393 580 240 396 2.88e-11 67.0
MsaG029810 AT5G61190.5 27.128 188 106 5 393 580 240 396 2.90e-11 67.0
MsaG029810 AT5G61190.6 27.128 188 106 5 393 580 240 396 2.93e-11 67.0
MsaG029810 AT5G61190.9 27.128 188 106 5 393 580 240 396 3.12e-11 67.0

Find 124 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTGGCAACTTCAAATTTA+TGG 0.103793 5:-81542570 MsaT029810.1:CDS
CTTCTTAGGCTCTGATTGTT+TGG 0.209399 5:+81542158 None:intergenic
ACAGAATCAACCTGTTTCTT+AGG 0.226882 5:+81542048 None:intergenic
TTCGGCTAAAGGACCAATTA+AGG 0.271027 5:+81541792 None:intergenic
ACAAAATCAACCTGCTTCTT+AGG 0.280074 5:+81542144 None:intergenic
TTCAATGCTTCTAATATTGC+AGG 0.282177 5:-81542829 None:intergenic
TGCGCAGAAGGCAAACTTGC+AGG 0.288386 5:+81542687 None:intergenic
TTCTTAGGCTCTGATTGTTT+GGG 0.304970 5:+81542159 None:intergenic
TTTCTTAGGCTCTGATTGTT+TGG 0.305574 5:+81541870 None:intergenic
ATACTATCAACCTGTTTCTT+AGG 0.309233 5:+81542306 None:intergenic
CCGCTGCCAACACCGACTTT+TGG 0.320862 5:+81539984 None:intergenic
ACAGAATCAACCTGTTTCTT+AGG 0.324318 5:+81541664 None:intergenic
ACAGAATCAACCTGTTTCTT+AGG 0.324318 5:+81541760 None:intergenic
ACAGAATCAACCTGTTTCTT+AGG 0.324318 5:+81541856 None:intergenic
ATCCAAGGACAAACATTAAT+TGG 0.324738 5:-81542093 MsaT029810.1:CDS
CTTCTGCACGTAATGGTTCC+TGG 0.342961 5:-81542587 MsaT029810.1:CDS
ACCGCAATAGGCACTTCAAT+TGG 0.345252 5:-81539945 MsaT029810.1:CDS
TCACCATAGCAGCATGATTA+TGG 0.346041 5:+81539745 None:intergenic
ACGGAATCAACCTGTTTCTT+AGG 0.353929 5:+81542240 None:intergenic
CGTGCAGAAGGCAAACTTGC+AGG 0.359812 5:+81542600 None:intergenic
GTTCTTCAAGTGTTTCTTAC+CGG 0.360756 5:+81540025 None:intergenic
ATCCAAGGACAAGCGTTAAT+TGG 0.362815 5:-81541709 MsaT029810.1:CDS
GCCAATGCTTCTAATATTGC+CGG 0.369202 5:-81542655 MsaT029810.1:CDS
ATCCAAGGACAAGCATTAAT+TGG 0.369738 5:-81541901 MsaT029810.1:CDS
ATCCAATCACAAGCCTTAAT+TGG 0.371873 5:-81541805 MsaT029810.1:CDS
GAAACAGGTTGATTCTGTTA+AGG 0.378526 5:-81541659 MsaT029810.1:CDS
ATCCAAGGTCAAGCGTTAAT+TGG 0.378876 5:-81542351 MsaT029810.1:CDS
CGCTTGAGTTCGGCAATAGA+TGG 0.381144 5:+81540164 None:intergenic
GGCTCTGATTGTTCGGCTAC+AGG 0.383641 5:+81541973 None:intergenic
TTTCTTAGGCTCTGATTGTT+CGG 0.390268 5:+81541678 None:intergenic
TTTCTTAGGCTCTGATTGTT+CGG 0.390268 5:+81541774 None:intergenic
TTTCTTAGGCTCTGATTGTT+CGG 0.390268 5:+81542254 None:intergenic
GGCTCTGATTGTTCGGCTAC+AGG 0.393801 5:+81542261 None:intergenic
TGAACAGAAGGCAAACTTGC+AGG 0.394062 5:+81542774 None:intergenic
GGCTCTGATTGTTCGGCTAT+AGG 0.407509 5:+81541685 None:intergenic
GCAATCAGAGCCTAAGAAAC+AGG 0.410131 5:-81542316 MsaT029810.1:CDS
AAGCTTTGCTGACTTTGGTT+TGG 0.410273 5:+81542520 None:intergenic
TCTGAAAGCTTTGCTGACTT+TGG 0.410594 5:+81542515 None:intergenic
GCCATAAATTTGAAGTTGCC+AGG 0.413010 5:+81542569 None:intergenic
GGCTCTGATTGTTCGGCTAA+AGG 0.413854 5:+81541781 None:intergenic
GACTGCAAAATTACAAGCAT+TGG 0.415529 5:+81542741 None:intergenic
GACCAATTAATGTTTGTCCT+TGG 0.416054 5:+81542091 None:intergenic
ACTACCACCACCACCGCAAT+AGG 0.416616 5:-81539957 MsaT029810.1:CDS
ACAATCAGAGCCTAAGAAAC+AGG 0.424333 5:-81541866 MsaT029810.1:CDS
TTCGCATGAGATGCAATTGT+AGG 0.424733 5:+81542396 None:intergenic
GACCAATTAAGGCTTGTGAT+TGG 0.426163 5:+81541803 None:intergenic
GGTGATTATTTAAATTTGCC+TGG 0.434831 5:+81539775 None:intergenic
TTGCAGGCGCTGCTGAAGTC+TGG 0.435502 5:+81542616 None:intergenic
GCAATCAGATCCTAAGAAAC+AGG 0.437003 5:-81542058 MsaT029810.1:CDS
ACATTAATTGGTCCTGTAGC+CGG 0.438110 5:-81542081 MsaT029810.1:CDS
ACAATCAGAGCCTAAGAAAC+AGG 0.441726 5:-81541674 MsaT029810.1:CDS
ACAATCAGAGCCTAAGAAAC+AGG 0.441726 5:-81541770 MsaT029810.1:CDS
ACAATCAGAGCCTAAGAAAC+AGG 0.441726 5:-81542250 MsaT029810.1:CDS
GACCAATTAACGCTTGTCCT+TGG 0.442236 5:+81541707 None:intergenic
GGCTCTGATTGCTCGCCTAC+AGG 0.444213 5:+81542327 None:intergenic
AATGCCATCATTAGACTTCA+AGG 0.445716 5:+81540190 None:intergenic
GACCAATTAATGCTTGTCCT+TGG 0.452219 5:+81541899 None:intergenic
GGATCTGATTGCCCGGCTAC+AGG 0.453935 5:+81542069 None:intergenic
ACATATAACAAACATGTTGC+TGG 0.455549 5:-81541598 MsaT029810.1:CDS
GACCGATTAACGCTTGTCCT+TGG 0.456245 5:+81541995 None:intergenic
TCCTTGGATCCTACTTGTGT+CGG 0.456469 5:+81542011 None:intergenic
TCCTTCAATACTTCTTGTGT+CGG 0.457555 5:+81542203 None:intergenic
AGCTACACTGCACACTTGTC+CGG 0.457647 5:-81540044 MsaT029810.1:CDS
ATCCAAGGACAAGCGTTAAT+CGG 0.462764 5:-81541997 MsaT029810.1:CDS
ATCACCGTTACGCTTGAGTT+CGG 0.470795 5:+81540154 None:intergenic
ACCGACACAAGAAGTATTGA+AGG 0.474071 5:-81542204 MsaT029810.1:CDS
CTTCTAATATTGCCGGCTGT+TGG 0.475497 5:-81542648 MsaT029810.1:CDS
CTTCTAATATTGCAGGCTGT+TGG 0.480500 5:-81542822 MsaT029810.1:CDS
GCCGGCAATATTAGAAGCAT+TGG 0.481716 5:+81542654 None:intergenic
CATTAATTGGTCCTGTAGCC+GGG 0.486089 5:-81542080 MsaT029810.1:CDS
TTGCTGACTTTGGTTTGGTT+AGG 0.492552 5:+81542525 None:intergenic
CCGCAATAGGCACTTCAATT+GGG 0.497753 5:-81539944 MsaT029810.1:CDS
TATTGCGGTGGTGGTGGTAG+TGG 0.498143 5:+81539959 None:intergenic
AGTTTGCCTTCTGCACGTAA+TGG 0.498221 5:-81542594 MsaT029810.1:CDS
CTTGCAAAATTCACACCATA+CGG 0.505040 5:+81540088 None:intergenic
GACCAATTAACGCTTGACCT+TGG 0.509177 5:+81542349 None:intergenic
AGTTTGCCTTCTGTTCAGAA+TGG 0.514605 5:-81542768 MsaT029810.1:CDS
CATATAACAAACATGTTGCT+GGG 0.516681 5:-81541597 MsaT029810.1:CDS
AGGCACTTCAATTGGGCCAC+AGG 0.517728 5:-81539937 MsaT029810.1:CDS
TGAAGTGCCTATTGCGGTGG+TGG 0.518303 5:+81539950 None:intergenic
CAGCATGATTATGGTCACTA+AGG 0.520547 5:+81539754 None:intergenic
TCATGCTGCTATGGTGAAGA+AGG 0.523999 5:-81539739 MsaT029810.1:CDS
GGCATACCATTCTGCGCAGA+AGG 0.536308 5:+81542675 None:intergenic
TTTCTTAGGATCTGATTGCC+CGG 0.536513 5:+81542062 None:intergenic
GCTGCTATGGTGAAGAAGGC+AGG 0.537781 5:-81539735 MsaT029810.1:CDS
CCAAAAGTCGGTGTTGGCAG+CGG 0.541495 5:-81539984 MsaT029810.1:CDS
ACAATCAGAGCCTAAGAAGC+AGG 0.543600 5:-81542154 MsaT029810.1:CDS
AAAAGCTGCTAAGAAAATCA+AGG 0.554241 5:-81540120 MsaT029810.1:CDS
TGGGAGAAAGCATGCTGCTA+AGG 0.554649 5:-81541578 MsaT029810.1:intron
AAACGTAAAGCTGTGGAAAG+AGG 0.559914 5:-81539852 MsaT029810.1:CDS
AAGAAGGCAGGGTTAATCAC+TGG 0.566902 5:-81539723 MsaT029810.1:CDS
GTCGGTTTGTACATTCTCAA+CGG 0.567858 5:+81542221 None:intergenic
TGACCATAATCATGCTGCTA+TGG 0.569530 5:-81539748 MsaT029810.1:CDS
GGAATACCATTCTGAACAGA+AGG 0.573481 5:+81542762 None:intergenic
AGTGCCTATTGCGGTGGTGG+TGG 0.576879 5:+81539953 None:intergenic
AGTTTGCCTTCTGCGCAGAA+TGG 0.578105 5:-81542681 MsaT029810.1:CDS
TCAAATCCAAAAGTCGGTGT+TGG 0.581990 5:-81539990 MsaT029810.1:CDS
ATGTACAAACCGACACAAGT+AGG 0.584340 5:-81542020 MsaT029810.1:CDS
CAATGTGGTGTGTAATAGCC+AGG 0.584891 5:-81539793 MsaT029810.1:CDS
AAACAGGTTGATAGTATACA+AGG 0.585879 5:-81542300 MsaT029810.1:CDS
GTTGCCTTGAAGTCTAATGA+TGG 0.591860 5:-81540194 MsaT029810.1:CDS
ACATGCAATGTTGTATGCGT+TGG 0.594466 5:-81541628 MsaT029810.1:CDS
GCAGGGTTAATCACTGGTTG+AGG 0.595222 5:-81539717 None:intergenic
CTGCTATGGTGAAGAAGGCA+GGG 0.602051 5:-81539734 MsaT029810.1:CDS
GACAGAGAAACGTAAAGCTG+TGG 0.602727 5:-81539859 MsaT029810.1:CDS
CAGGAACCATTACGTGCAGA+AGG 0.603247 5:+81542588 None:intergenic
CAAGCGTTAATTGGTCCTGT+AGG 0.605906 5:-81542342 MsaT029810.1:CDS
TTGAAGTCTAATGATGGCAT+TGG 0.606462 5:-81540188 MsaT029810.1:CDS
ACTGACACAAGTAGTATCCA+AGG 0.607332 5:-81541916 MsaT029810.1:CDS
ACTGACACAAGTAGTATCCA+AGG 0.607332 5:-81542108 MsaT029810.1:CDS
TCAAGGTTGATGAATCCGTA+TGG 0.610274 5:-81540103 MsaT029810.1:CDS
ACTGACACAAGTAGTATCCA+AGG 0.612630 5:-81542366 MsaT029810.1:CDS
TGGTGTGATGAACCAACAGC+CGG 0.618587 5:+81542636 None:intergenic
ACCGACACAAGTAGGATCCA+AGG 0.619525 5:-81542012 MsaT029810.1:CDS
ACTAACTCAAGTAATATCCA+AGG 0.626304 5:-81541724 MsaT029810.1:CDS
TTCAGATAAACCGAAAACAA+AGG 0.627362 5:-81539904 MsaT029810.1:CDS
CCCAATTGAAGTGCCTATTG+CGG 0.632472 5:+81539944 None:intergenic
AAACTGTCAAATCCAAAAGT+CGG 0.636332 5:-81539996 MsaT029810.1:CDS
TAAGAAACACTTGAAGAACA+TGG 0.658000 5:-81540021 MsaT029810.1:CDS
AACACAAGAAGAATTTGCAA+CGG 0.663613 5:-81542428 MsaT029810.1:CDS
AATTGAAGTGCCTATTGCGG+TGG 0.664606 5:+81539947 None:intergenic
ATTGCCGAACTCAAGCGTAA+CGG 0.670672 5:-81540158 MsaT029810.1:CDS
AGATAAACCGAAAACAAAGG+AGG 0.682827 5:-81539901 MsaT029810.1:CDS
ATTGTGCACTTTATGCAATG+TGG 0.685678 5:-81539808 MsaT029810.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 81539719 81542847 81539719 ID=MsaG029810
Chr5 mRNA 81539719 81542847 81539719 ID=MsaT029810.1;Parent=MsaG029810
Chr5 exon 81539719 81540216 81539719 ID=MsaT029810.1.exon2;Parent=MsaT029810.1
Chr5 CDS 81539719 81540216 81539719 ID=cds.MsaT029810.1;Parent=MsaT029810.1
Chr5 exon 81541579 81542847 81541579 ID=MsaT029810.1.exon1;Parent=MsaT029810.1
Chr5 CDS 81541579 81542847 81541579 ID=cds.MsaT029810.1;Parent=MsaT029810.1
Gene Sequence

>MsaT029810.1

ATGCTTCTAATATTGCAGGCTGTTGGTTCATCACACGAGACTTCAGCAGTGCCTGCAAGTTTGCCTTCTGTTCAGAATGGTATTCCAATGCTTGTAATTTTGCAGTCTGCTGGTTCATCACACGAGACTTCAGCAGCGCCTGCAAGTTTGCCTTCTGCGCAGAATGGTATGCCAATGCTTCTAATATTGCCGGCTGTTGGTTCATCACACCAGACTTCAGCAGCGCCTGCAAGTTTGCCTTCTGCACGTAATGGTTCCTGGCAACTTCAAATTTATGGCAATCAGACTACAATAATGTTGCCTAACCAAACCAAAGTCAGCAAAGCTTTCAGATGTGAAGTGTGTGAAATTGATGTCAATAGCCAAGTCTCTTTTGAAAAGCACATAGCTGGAAAGAAACACAAGAAGAATTTGCAACGGCAGACTAATCCTACAATTGCATCTCATGCGAATTTACAAACTGACACAAGTAGTATCCAAGGTCAAGCGTTAATTGGTCCTGTAGGCGAGCAATCAGAGCCTAAGAAACAGGTTGATAGTATACAAGGACAAGCTTTAATCTGTCCTGTAGCCGAACAATCAGAGCCTAAGAAACAGGTTGATTCCGTTGAGAATGTACAAACCGACACAAGAAGTATTGAAGGACAAGCGTTAATTGTTGCTGTACCCAAACAATCAGAGCCTAAGAAGCAGGTTGATTTTGTTAAGAATGTACAAACTGACACAAGTAGTATCCAAGGACAAACATTAATTGGTCCTGTAGCCGGGCAATCAGATCCTAAGAAACAGGTTGATTCTGTTAAGAATGTACAAACCGACACAAGTAGGATCCAAGGACAAGCGTTAATCGGTCCTGTAGCCGAACAATCAGAGCCTAAAAAACAGGTTGATTCTGTTAAGAATGTACAAACTGACACAAGTAGTATCCAAGGACAAGCATTAATTGGTCTTGTAGCCAAACAATCAGAGCCTAAGAAACAGGTTGATTCTGTTAAGAATGTACAAACTGACACAAGTAGTATCCAATCACAAGCCTTAATTGGTCCTTTAGCCGAACAATCAGAGCCTAAGAAACAGGTTGATTCTGTTAAGAATGTACAAACTAACTCAAGTAATATCCAAGGACAAGCGTTAATTGGTCCTATAGCCGAACAATCAGAGCCTAAGAAACAGGTTGATTCTGTTAAGGTTTGCTCAACATGCAATGTTGTATGCGTTGGTCAAGATACATATAACAAACATGTTGCTGGGAGAAAGCATGCTGCTAAGGTTGCCTTGAAGTCTAATGATGGCATTGGTCCATCTATTGCCGAACTCAAGCGTAACGGTGATGCTCTAATTGAAAAAGCTGCTAAGAAAATCAAGGTTGATGAATCCGTATGGTGTGAATTTTGCAAGATTAATTGTAACAGCAAGTATAGCTACACTGCACACTTGTCCGGTAAGAAACACTTGAAGAACATGGAGAAACTGTCAAATCCAAAAGTCGGTGTTGGCAGCGGTGCCACTACCACCACCACCGCAATAGGCACTTCAATTGGGCCACAGGAAAAGCCAGATTCAGATAAACCGAAAACAAAGGAGGTATCAGTACTTGATATCGAGACAGAGAAACGTAAAGCTGTGGAAAGAGGAGCTGCAGTCGATGATATTAAATTGTGCACTTTATGCAATGTGGTGTGTAATAGCCAGGCAAATTTAAATAATCACCTTAGTGACCATAATCATGCTGCTATGGTGAAGAAGGCAGGGTTAATCACTGGTTGA

Protein sequence

>MsaT029810.1

MLLILQAVGSSHETSAVPASLPSVQNGIPMLVILQSAGSSHETSAAPASLPSAQNGMPMLLILPAVGSSHQTSAAPASLPSARNGSWQLQIYGNQTTIMLPNQTKVSKAFRCEVCEIDVNSQVSFEKHIAGKKHKKNLQRQTNPTIASHANLQTDTSSIQGQALIGPVGEQSEPKKQVDSIQGQALICPVAEQSEPKKQVDSVENVQTDTRSIEGQALIVAVPKQSEPKKQVDFVKNVQTDTSSIQGQTLIGPVAGQSDPKKQVDSVKNVQTDTSRIQGQALIGPVAEQSEPKKQVDSVKNVQTDTSSIQGQALIGLVAKQSEPKKQVDSVKNVQTDTSSIQSQALIGPLAEQSEPKKQVDSVKNVQTNSSNIQGQALIGPIAEQSEPKKQVDSVKVCSTCNVVCVGQDTYNKHVAGRKHAAKVALKSNDGIGPSIAELKRNGDALIEKAAKKIKVDESVWCEFCKINCNSKYSYTAHLSGKKHLKNMEKLSNPKVGVGSGATTTTTAIGTSIGPQEKPDSDKPKTKEVSVLDIETEKRKAVERGAAVDDIKLCTLCNVVCNSQANLNNHLSDHNHAAMVKKAGLITG*