AlfalfaGEDB | MsaG029859

M. sativa ssp. caerulea / MsaG029859

KEGG: -
GO: -
PFAM: PF13912.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG029859	tr\|G7K0M6\|G7K0M6_MEDTR	89.630	270	26	1	1	268	1	270	5.59e-173	487

TFs/TRs:

Gene ID	Type	Classification
MsaG029859	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000034	MsaG029859	0.809184	3.659188e-50	6.126905e-48
MsaG001156	MsaG029859	0.806047	1.684371e-49	2.616804e-47
MsaG001179	MsaG029859	0.830256	5.850773e-55	1.705662e-52
MsaG002016	MsaG029859	0.807092	1.015795e-49	1.617557e-47
MsaG004202	MsaG029859	0.800473	2.370952e-48	3.240108e-46
MsaG006456	MsaG029859	0.800950	1.896587e-48	2.619889e-46
MsaG006533	MsaG029859	0.814640	2.402036e-51	4.601300e-49
MsaG007429	MsaG029859	0.813157	5.080753e-51	9.377436e-49
MsaG008465	MsaG029859	0.801243	1.653286e-48	2.298921e-46
MsaG011114	MsaG029859	0.800979	1.871599e-48	2.587005e-46
MsaG014956	MsaG029859	0.812768	6.176947e-51	1.129027e-48
MsaG016724	MsaG029859	0.826836	3.884306e-54	1.028132e-51
MsaG018452	MsaG029859	0.802162	1.073177e-48	1.523784e-46
MsaG019182	MsaG029859	0.802519	9.068533e-49	1.298238e-46
MsaG020314	MsaG029859	0.823312	2.617535e-53	6.288916e-51
MsaG021405	MsaG029859	0.816999	7.195212e-52	1.463610e-49
MsaG021896	MsaG029859	0.809871	2.609659e-50	4.442658e-48
MsaG022438	MsaG029859	0.815846	1.299920e-51	2.567258e-49
MsaG022441	MsaG029859	0.834115	6.564582e-56	2.141356e-53
MsaG023591	MsaG029859	0.809780	2.729593e-50	4.636735e-48
MsaG023882	MsaG029859	0.809107	3.799955e-50	6.351074e-48
MsaG024015	MsaG029859	0.810041	2.399182e-50	4.101243e-48
MsaG029859	MsaG029862	0.954922	3.339776e-112	1.123357e-106
MsaG029859	MsaG029910	0.800001	2.954186e-48	3.994521e-46
MsaG029859	MsaG030840	0.832983	1.254184e-55	3.957151e-53
MsaG029859	MsaG031436	0.825907	6.452019e-54	1.664277e-51
MsaG029859	MsaG034658	0.811664	1.073060e-50	1.908527e-48
MsaG029859	MsaG037853	0.815193	1.813571e-51	3.523010e-49
MsaG029859	MsaG040775	0.820430	1.207563e-52	2.686380e-50
MsaG029859	MsaG041368	0.830040	6.601612e-55	1.912771e-52
MsaG029859	MsaG044131	0.819916	1.581514e-52	3.470540e-50
MsaG029859	MsaG044352	0.800477	2.366341e-48	3.234117e-46
MsaG029859	MsaG044354	0.822432	4.187944e-53	9.825315e-51
MsaG029859	MsaG046307	0.832171	1.990202e-55	6.132705e-53
MsaG029859	MsaG046630	0.803629	5.358700e-49	7.869868e-47
MsaG029859	MsaG019037	0.816446	9.560966e-52	1.917284e-49
MsaG029859	MsaG019111	0.843268	2.900283e-58	1.256140e-55
MsaG029859	MsaG019920	0.839076	3.624477e-57	1.374607e-54
MsaG029859	MsaG032859	0.811712	1.047574e-50	1.865407e-48
MsaG029859	MsaG032481	0.806265	1.515728e-49	2.367152e-47
MsaG029859	MsaG038716	0.826387	4.965688e-54	1.298066e-51
MsaG029859	MsaG037163	0.804179	4.124705e-49	6.135432e-47
MsaG029859	MsaG045256	0.814760	2.259910e-51	4.342306e-49
MsaG029859	MsaG043831	0.831466	2.964361e-55	8.948421e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG029859	MtrunA17_Chr5g0448221	89.630	270	26	1	1	268	1	270	1.08e-176	487
MsaG029859	MtrunA17_Chr1g0169561	32.039	206	84	9	44	202	14	210	1.63e-13	69.3
MsaG029859	MtrunA17_Chr2g0331601	30.000	170	75	5	62	207	138	287	1.64e-13	69.3
MsaG029859	MtrunA17_Chr1g0169521	31.282	195	85	7	49	202	24	210	3.78e-12	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG029859	AT5G56200.1	44.048	84	41	1	60	137	77	160	1.22e-12	67.8
MsaG029859	AT2G26940.1	33.981	103	43	1	62	139	49	151	6.46e-12	64.7

Find 51 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAATATTATTATCCTTATT+AGG	0.131951	5:+82388144	None:intergenic
ATACCCCTCCATTCCCTTTC+AGG	0.172402	5:+82388171	None:intergenic
GTGACCATGTAAAGCCTTAT+TGG	0.177322	5:+82388206	None:intergenic
ATCATCAATAACCAATTCTT+TGG	0.220906	5:+82388074	None:intergenic
ATCAAAAGGTAATGTCTTAT+TGG	0.231745	5:-82387779	MsaT029859.1:CDS
CAATATTATTATCCTTATTA+GGG	0.247792	5:+82388145	None:intergenic
CATCAAACTTATTCTTCTTT+TGG	0.256389	5:+82388634	None:intergenic
AATAACCAATTCTTTGGAAT+TGG	0.272420	5:+82388080	None:intergenic
TCATCTTGATGATGATCCTT+TGG	0.317224	5:+82388420	None:intergenic
TTTAGGTGTGGAAAAGGATT+AGG	0.327731	5:-82388348	MsaT029859.1:CDS
TGCATCCAATTCCAAAGAAT+TGG	0.334606	5:-82388085	MsaT029859.1:CDS
CTTTCCCTAGAGCTTGACTA+TGG	0.341322	5:+82388310	None:intergenic
TGACAACTTCAATGAGGATT+TGG	0.347180	5:-82387935	MsaT029859.1:CDS
TTGATGATGAATCAAAATCT+TGG	0.371768	5:-82388059	MsaT029859.1:CDS
GAGGGGTATGAACCCTAATA+AGG	0.375613	5:-82388157	MsaT029859.1:CDS
TACCCCTCCATTCCCTTTCA+GGG	0.375926	5:+82388172	None:intergenic
AAAGAACTTCCAATCCAATA+AGG	0.383910	5:-82388220	MsaT029859.1:CDS
GTTGTCATGTAAATGTTTAT+TGG	0.397142	5:+82387951	None:intergenic
TGATGATGAATCAAAATCTT+GGG	0.427675	5:-82388058	MsaT029859.1:CDS
CATGTAAAGCCTTATTGGAT+TGG	0.438633	5:+82388211	None:intergenic
CAATCCAATAAGGCTTTACA+TGG	0.457233	5:-82388210	MsaT029859.1:CDS
GCTAATTGCAATTCTAATGA+TGG	0.457837	5:-82388267	MsaT029859.1:CDS
GCACGAAGATTGTTTAAAAC+CGG	0.468350	5:-82388036	MsaT029859.1:CDS
CCTAGTACTAGTGGTCTTGT+TGG	0.468849	5:-82387883	MsaT029859.1:CDS
CAAAAGAAGAATAAGTTTGA+TGG	0.471977	5:-82388633	MsaT029859.1:CDS
AGAATCCATAGTCAAGCTCT+AGG	0.477759	5:-82388315	MsaT029859.1:CDS
AGTCCATGTTCAACATCAAA+AGG	0.485835	5:-82387793	MsaT029859.1:CDS
TTTGGATCATGAGGATAAGA+AGG	0.488315	5:-82387917	MsaT029859.1:CDS
CATGAGATCTCACCCTGAAA+GGG	0.502988	5:-82388184	MsaT029859.1:CDS
CCAACAAGACCACTAGTACT+AGG	0.509402	5:+82387883	None:intergenic
TTGTTTAAAACCGGTAAAAG+AGG	0.519380	5:-82388027	MsaT029859.1:CDS
ACATGAGATCTCACCCTGAA+AGG	0.521597	5:-82388185	MsaT029859.1:CDS
AAGGATCATCATCAAGATGA+AGG	0.523519	5:-82388417	MsaT029859.1:CDS
TGATGGTGGTAGGATACATC+AGG	0.528563	5:-82388616	MsaT029859.1:intron
TAGTCAAGCTCTAGGGAAAG+AGG	0.530133	5:-82388307	MsaT029859.1:CDS
AGAATGACAATAACAAAAGA+AGG	0.532053	5:-82387837	MsaT029859.1:CDS
AGGTGTGGAAAAGGATTAGG+TGG	0.533754	5:-82388345	MsaT029859.1:CDS
AGAATAAGTTTGATGGTGGT+AGG	0.553715	5:-82388626	MsaT029859.1:CDS
CAATGAGGATTTGGATCATG+AGG	0.559595	5:-82387926	MsaT029859.1:CDS
GATCTCACCCTGAAAGGGAA+TGG	0.563550	5:-82388179	MsaT029859.1:CDS
GAATCCATAGTCAAGCTCTA+GGG	0.567403	5:-82388314	MsaT029859.1:CDS
TCACCCTGAAAGGGAATGGA+GGG	0.580204	5:-82388175	MsaT029859.1:CDS
CTCACCCTGAAAGGGAATGG+AGG	0.583418	5:-82388176	MsaT029859.1:CDS
TAACAAAAGAAGGAAGAAGC+TGG	0.586820	5:-82387827	MsaT029859.1:CDS
TTTACATGACAACTTCAATG+AGG	0.602810	5:-82387941	MsaT029859.1:CDS
GGTAAAAGAGGTCGTTCGAG+TGG	0.603484	5:-82388015	MsaT029859.1:CDS
AGTCAAGCTCTAGGGAAAGA+GGG	0.609576	5:-82388306	MsaT029859.1:CDS
AATATTGTACCTAGTACTAG+TGG	0.612564	5:-82387892	MsaT029859.1:CDS
AAGAAGAATAAGTTTGATGG+TGG	0.622051	5:-82388630	MsaT029859.1:CDS
CACCCTGAAAGGGAATGGAG+GGG	0.655548	5:-82388174	MsaT029859.1:CDS
AGAGGGTGAGTCGAAAGCAA+AGG	0.661254	5:-82388289	MsaT029859.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	82387774	82388670	82387774	ID=MsaG029859
Chr5	mRNA	82387774	82388670	82387774	ID=MsaT029859.1;Parent=MsaG029859
Chr5	exon	82387774	82388526	82387774	ID=MsaT029859.1.exon2;Parent=MsaT029859.1
Chr5	CDS	82387774	82388526	82387774	ID=cds.MsaT029859.1;Parent=MsaT029859.1
Chr5	exon	82388617	82388670	82388617	ID=MsaT029859.1.exon1;Parent=MsaT029859.1
Chr5	CDS	82388617	82388670	82388617	ID=cds.MsaT029859.1;Parent=MsaT029859.1

Gene Sequence

>MsaT029859.1

ATGGATCCCAATGACCAAAAGAAGAATAAGTTTGATGGTGGTAGGATACATCAGAATTCTAGGGCAAATTCCATGAAAACCATAGAGAAGAAGAAAAGTTTAGCATCAATTTCTAAGTCTTGTAAAGTTTATGAAAAACCAAAGGATCATCATCAAGATGAAGGATCAACAAGTTCAAAAATTCACAAGTGTGAATTTTGTAACAAAATTTTTAGGTGTGGAAAAGGATTAGGTGGTCACAAAAGAATCCATAGTCAAGCTCTAGGGAAAGAGGGTGAGTCGAAAGCAAAGGCTAATTGCAATTCTAATGATGGAAAATTAAGTTGTGATGTTTGCAAAAAGAACTTCCAATCCAATAAGGCTTTACATGGTCACATGAGATCTCACCCTGAAAGGGAATGGAGGGGTATGAACCCTAATAAGGATAATAATATTGATTATTATAATGATGATCAAGATCAATATGTTCTTAGTGCATCCAATTCCAAAGAATTGGTTATTGATGATGAATCAAAATCTTGGGCACGAAGATTGTTTAAAACCGGTAAAAGAGGTCGTTCGAGTGGTGAAGTTGTTATTGCTGCTCAAATACTCGTGTATATGTCTACCAATAAACATTTACATGACAACTTCAATGAGGATTTGGATCATGAGGATAAGAAGGAGAATATTGTACCTAGTACTAGTGGTCTTGTTGGTGACATTGATGAAACAAAATTGAAGAATGACAATAACAAAAGAAGGAAGAAGCTGGTGAAATTGATGAGTCCATGTTCAACATCAAAAGGTAATGTCTTATTGGACTAA

Protein sequence

>MsaT029859.1

MDPNDQKKNKFDGGRIHQNSRANSMKTIEKKKSLASISKSCKVYEKPKDHHQDEGSTSSKIHKCEFCNKIFRCGKGLGGHKRIHSQALGKEGESKAKANCNSNDGKLSCDVCKKNFQSNKALHGHMRSHPEREWRGMNPNKDNNIDYYNDDQDQYVLSASNSKELVIDDESKSWARRLFKTGKRGRSSGEVVIAAQILVYMSTNKHLHDNFNEDLDHEDKKENIVPSTSGLVGDIDETKLKNDNNKRRKKLVKLMSPCSTSKGNVLLD*