AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029878


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029878 MtrunA17_Chr5g0448431 98.780 164 2 0 1 164 1 164 1.00e-120 337
MsaG029878 MtrunA17_Chr5g0395301 27.632 152 87 3 14 142 17 168 4.98e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029878 AT5G11340.1 78.049 164 36 0 1 164 1 164 2.53e-97 278
MsaG029878 AT5G16800.1 23.926 163 101 3 4 143 20 182 2.16e-12 63.2
MsaG029878 AT5G16800.2 23.926 163 101 3 4 143 20 182 4.01e-12 62.8
MsaG029878 AT5G16800.3 23.926 163 101 3 4 143 20 182 5.87e-12 62.4

Find 28 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTTCTTATAAAAGTTAA+TGG 0.199299 5:+82674802 None:intergenic
TGATGTTTACGTAATAGTTC+TGG 0.333615 5:+82674756 None:intergenic
CGGGTTTACATCATGACTTT+AGG 0.340929 5:-82675198 MsaT029878.1:CDS
AGAAGGAAGGTGGGCAAGTT+CGG 0.349914 5:-82675218 MsaT029878.1:CDS
GAAGGAAGGTGGGCAAGTTC+GGG 0.372201 5:-82675217 MsaT029878.1:CDS
TATTTGTCGTTGTAACGAAC+AGG 0.385879 5:+82677988 None:intergenic
GGATAGGAAAGAACCTAGTT+TGG 0.407491 5:+82677933 None:intergenic
CCTTACCGTGGACTTGGTAT+TGG 0.408366 5:-82675165 MsaT029878.1:intron
GAAGTATCAATCTCATTAGA+TGG 0.418274 5:-82678063 MsaT029878.1:CDS
TTAGCGCCTTACCGTGGACT+TGG 0.429752 5:-82675171 MsaT029878.1:CDS
TGCGTGCCGGCTTGAGAAGA+AGG 0.483070 5:-82675235 MsaT029878.1:CDS
TCCGCTGATTTCACCAAACT+AGG 0.503020 5:-82677946 MsaT029878.1:intron
GTGTTGGCGCAATTGCGTGC+CGG 0.514629 5:-82675248 MsaT029878.1:CDS
CACCTTCCTTCTTCTCAAGC+CGG 0.529491 5:+82675229 None:intergenic
CGCGAAGCAGAACATTTCTG+AGG 0.551841 5:-82674858 MsaT029878.1:CDS
TACTACAGTGACATTTGTGT+TGG 0.556487 5:-82675264 MsaT029878.1:CDS
TTTGGGTTTATGCAGGAACA+AGG 0.566199 5:-82674907 MsaT029878.1:intron
GAAGTAGAATCAATGGGAGC+TGG 0.568816 5:-82678090 None:intergenic
CGGCTTGAGAAGAAGGAAGG+TGG 0.571876 5:-82675228 MsaT029878.1:CDS
GGCTTGAGAAGAAGGAAGGT+GGG 0.574382 5:-82675227 MsaT029878.1:CDS
CTTGTGACAACATAACAGTC+TGG 0.600627 5:+82674728 None:intergenic
TGCCGGCTTGAGAAGAAGGA+AGG 0.603686 5:-82675231 MsaT029878.1:CDS
CCAATACCAAGTCCACGGTA+AGG 0.611714 5:+82675165 None:intergenic
ATTTGTCGTTGTAACGAACA+GGG 0.627678 5:+82677989 None:intergenic
TCTTACCAATACCAAGTCCA+CGG 0.630487 5:+82675160 None:intergenic
ACCTAGTTTGGTGAAATCAG+CGG 0.645414 5:+82677945 None:intergenic
CAGACTGTTATGTTGTCACA+AGG 0.649227 5:-82674727 MsaT029878.1:CDS
GTGACAACATAACAGTCTGG+TGG 0.757743 5:+82674731 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 82674697 82678100 82674697 ID=MsaG029878
Chr5 mRNA 82674697 82678100 82674697 ID=MsaT029878.1;Parent=MsaG029878
Chr5 exon 82674697 82674914 82674697 ID=MsaT029878.1.exon3;Parent=MsaT029878.1
Chr5 CDS 82674697 82674914 82674697 ID=cds.MsaT029878.1;Parent=MsaT029878.1
Chr5 exon 82675166 82675288 82675166 ID=MsaT029878.1.exon2;Parent=MsaT029878.1
Chr5 CDS 82675166 82675288 82675166 ID=cds.MsaT029878.1;Parent=MsaT029878.1
Chr5 exon 82677947 82678100 82677947 ID=MsaT029878.1.exon1;Parent=MsaT029878.1
Chr5 CDS 82677947 82678100 82677947 ID=cds.MsaT029878.1;Parent=MsaT029878.1
Gene Sequence

>MsaT029878.1

ATGGGAGCTGGTCGTGAAGTATCAATCTCATTAGATGGAGTAAGAGACAAGAACATTATGCAACTCAAGAAGCTTAATCTTGCTCTTTTCCCTGTTCGTTACAACGACAAATACTATGCTGATGCTCTTGCTTCCGCTGATTTCACCAAACTAGCGTACTACAGTGACATTTGTGTTGGCGCAATTGCGTGCCGGCTTGAGAAGAAGGAAGGTGGGCAAGTTCGGGTTTACATCATGACTTTAGGTGTTTTAGCGCCTTACCGTGGACTTGGTATTGGAACAAGGCTGCTGAATCATGTTATTGATCTTTGCGCGAAGCAGAACATTTCTGAGGTTTACTTGCATGTGCAGACAAACAACGAAGATGCCATTAACTTTTATAAGAAATTTGAGTTTGAAATTACAGAAACTATCCAGAACTATTACGTAAACATCACACCACCAGACTGTTATGTTGTCACAAGGCACATAGCACCTAGCCCAACAAAAAAATAA

Protein sequence

>MsaT029878.1

MGAGREVSISLDGVRDKNIMQLKKLNLALFPVRYNDKYYADALASADFTKLAYYSDICVGAIACRLEKKEGGQVRVYIMTLGVLAPYRGLGIGTRLLNHVIDLCAKQNISEVYLHVQTNNEDAINFYKKFEFEITETIQNYYVNITPPDCYVVTRHIAPSPTKK*