AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029931

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029931 MtrunA17_Chr5g0448761 98.267 404 7 0 222 625 383 786 0.0 833
MsaG029931 MtrunA17_Chr5g0448761 94.595 222 9 2 1 222 1 219 7.90e-140 426
MsaG029931 MtrunA17_Chr5g0448511 52.109 403 177 3 225 623 158 548 4.14e-142 424
MsaG029931 MtrunA17_Chr3g0118611 49.387 326 161 2 215 540 254 575 7.20e-101 324
MsaG029931 MtrunA17_Chr5g0440451 44.478 335 182 2 215 549 357 687 2.06e-91 302
MsaG029931 MtrunA17_Chr5g0440451 32.778 180 95 6 26 205 15 168 1.03e-18 90.9
MsaG029931 MtrunA17_Chr1g0202341 38.835 309 176 2 222 523 367 669 7.03e-67 231
MsaG029931 MtrunA17_Chr1g0202341 41.667 168 93 3 56 223 44 206 7.78e-30 125
MsaG029931 MtrunA17_Chr3g0080351 34.591 318 201 3 226 543 386 696 1.68e-58 211
MsaG029931 MtrunA17_Chr3g0080351 38.519 135 62 3 55 174 68 196 3.57e-20 95.1
MsaG029931 MtrunA17_Chr2g0329031 33.746 323 202 4 222 544 321 631 2.94e-55 200
MsaG029931 MtrunA17_Chr2g0329031 45.833 120 57 4 62 181 13 124 3.98e-21 98.2
MsaG029931 MtrunA17_Chr1g0207551 34.426 305 194 2 222 526 348 646 1.17e-53 196
MsaG029931 MtrunA17_Chr1g0207551 32.955 176 111 3 44 219 15 183 1.55e-22 102
MsaG029931 MtrunA17_Chr2g0287061 37.241 290 175 3 237 525 476 759 3.49e-51 189
MsaG029931 MtrunA17_Chr2g0287061 38.889 108 49 2 61 168 181 271 4.77e-15 79.0
MsaG029931 MtrunA17_Chr2g0287061 36.449 107 51 2 62 168 28 117 7.52e-14 75.1
MsaG029931 MtrunA17_Chr5g0441581 34.295 312 182 8 249 552 363 659 1.32e-43 166
MsaG029931 MtrunA17_Chr2g0291581 32.203 295 190 5 244 530 394 686 4.77e-40 155
MsaG029931 MtrunA17_Chr6g0477261 38.034 234 139 3 242 469 217 450 5.27e-40 155
MsaG029931 MtrunA17_Chr3g0082121 29.651 344 234 4 188 530 320 656 2.80e-39 154
MsaG029931 MtrunA17_Chr3g0082121 38.037 163 98 1 60 222 40 199 9.59e-27 115
MsaG029931 MtrunA17_Chr2g0296781 37.333 225 129 3 254 469 340 561 7.56e-39 152
MsaG029931 MtrunA17_Chr1g0196541 35.965 228 140 3 251 472 337 564 2.30e-36 145
MsaG029931 MtrunA17_Chr8g0363841 31.739 230 155 2 238 465 349 578 3.94e-32 132
MsaG029931 MtrunA17_Chr6g0458391 30.126 239 166 1 226 464 528 765 5.46e-31 129
MsaG029931 MtrunA17_Chr6g0458391 45.161 124 64 2 63 186 225 344 1.12e-25 112
MsaG029931 MtrunA17_Chr6g0458391 34.694 147 84 4 61 200 89 230 9.19e-18 87.4
MsaG029931 MtrunA17_Chr1g0203721 25.879 313 217 3 222 527 382 686 6.96e-31 129
MsaG029931 MtrunA17_Chr1g0203721 32.847 137 88 2 66 202 66 198 6.84e-15 78.6
MsaG029931 MtrunA17_Chr2g0324011 24.290 317 218 5 152 467 276 571 1.58e-29 123
MsaG029931 MtrunA17_Chr2g0324011 33.742 163 103 4 60 222 7 164 1.98e-17 85.9
MsaG029931 MtrunA17_Chr4g0033611 42.857 154 76 4 24 170 30 178 1.43e-28 114
MsaG029931 MtrunA17_Chr3g0078041 52.778 108 48 2 62 169 49 153 3.54e-28 112
MsaG029931 MtrunA17_Chr1g0198761 29.508 244 161 4 234 476 387 620 1.13e-26 115
MsaG029931 MtrunA17_Chr1g0198761 31.937 191 105 7 37 208 14 198 1.13e-12 71.2
MsaG029931 MtrunA17_Chr2g0329421 54.128 109 45 3 62 170 26 129 2.13e-26 107
MsaG029931 MtrunA17_Chr2g0276841 28.912 294 188 8 234 523 405 681 2.49e-26 114
MsaG029931 MtrunA17_Chr2g0276841 32.432 148 91 4 63 205 70 213 1.00e-14 77.8
MsaG029931 MtrunA17_Chr2g0287651 27.632 228 164 1 238 464 405 632 4.27e-26 114
MsaG029931 MtrunA17_Chr2g0287651 35.032 157 100 2 67 223 79 233 4.81e-21 98.2
MsaG029931 MtrunA17_Chr2g0294841 25.424 295 209 5 234 528 383 666 5.88e-26 113
MsaG029931 MtrunA17_Chr2g0294841 33.529 170 104 5 60 222 42 209 3.22e-16 82.4
MsaG029931 MtrunA17_Chr7g0233911 51.852 108 49 3 62 169 33 137 6.31e-26 106
MsaG029931 MtrunA17_Chr2g0329361 41.985 131 67 3 61 191 59 180 7.93e-26 107
MsaG029931 MtrunA17_Chr2g0291591 31.351 185 123 2 220 400 370 554 2.24e-23 104
MsaG029931 MtrunA17_Chr2g0291591 33.333 141 76 6 62 197 63 190 1.11e-13 73.9
MsaG029931 MtrunA17_Chr2g0308841 23.982 221 167 1 244 463 6 226 3.46e-23 99.8
MsaG029931 MtrunA17_Chr1g0203051 25.346 217 161 1 237 452 406 622 4.04e-22 100
MsaG029931 MtrunA17_Chr1g0203051 31.410 156 103 3 69 223 85 237 2.66e-15 79.3
MsaG029931 MtrunA17_Chr2g0290391 25.068 367 241 11 181 533 330 676 8.17e-22 100
MsaG029931 MtrunA17_Chr2g0290391 35.829 187 102 6 37 213 22 200 6.37e-18 87.8
MsaG029931 MtrunA17_Chr7g0258861 33.537 164 105 3 60 223 7 166 8.14e-21 93.6
MsaG029931 MtrunA17_Chr5g0401121 39.640 111 64 2 63 173 56 163 1.18e-16 77.8
MsaG029931 MtrunA17_Chr2g0331371 39.683 126 68 4 61 182 51 172 3.27e-13 69.3
MsaG029931 MtrunA17_Chr1g0171741 33.621 116 70 3 67 180 137 247 6.93e-13 69.3
MsaG029931 MtrunA17_Chr5g0431001 27.624 181 117 5 55 223 50 228 3.11e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029931 AT4G38180.1 64.319 426 141 2 204 625 370 788 0.0 565
MsaG029931 AT4G38180.1 71.488 242 37 6 1 222 2 231 6.38e-95 309
MsaG029931 AT4G38170.1 43.925 428 222 4 201 623 131 545 4.25e-121 370
MsaG029931 AT2G27110.3 51.402 321 145 3 215 532 187 499 4.55e-107 339
MsaG029931 AT2G27110.2 51.572 318 149 2 215 532 332 644 8.89e-106 339
MsaG029931 AT2G27110.2 33.083 133 70 3 57 189 43 156 1.06e-14 78.2
MsaG029931 AT2G27110.1 51.572 318 149 2 215 532 332 644 8.89e-106 339
MsaG029931 AT2G27110.1 33.083 133 70 3 57 189 43 156 1.06e-14 78.2
MsaG029931 AT4G15090.1 33.333 330 214 2 222 551 368 691 1.70e-58 211
MsaG029931 AT4G15090.1 33.889 180 111 4 43 219 28 202 4.88e-23 104
MsaG029931 AT4G15090.5 33.333 330 214 2 222 551 368 691 1.70e-58 211
MsaG029931 AT4G15090.5 33.889 180 111 4 43 219 28 202 4.88e-23 104
MsaG029931 AT4G15090.3 33.333 330 214 2 222 551 368 691 1.70e-58 211
MsaG029931 AT4G15090.3 33.889 180 111 4 43 219 28 202 4.88e-23 104
MsaG029931 AT4G15090.2 33.333 330 214 2 222 551 368 691 1.70e-58 211
MsaG029931 AT4G15090.2 33.889 180 111 4 43 219 28 202 4.88e-23 104
MsaG029931 AT4G15090.4 33.333 330 214 2 222 551 368 691 1.70e-58 211
MsaG029931 AT4G15090.4 33.889 180 111 4 43 219 28 202 4.88e-23 104
MsaG029931 AT5G18960.2 37.072 321 195 4 204 523 470 784 1.91e-58 210
MsaG029931 AT5G18960.2 39.837 123 48 4 46 168 35 131 3.65e-17 85.9
MsaG029931 AT5G18960.2 38.532 109 50 2 62 170 210 301 2.74e-15 80.1
MsaG029931 AT5G18960.1 37.072 321 195 4 204 523 470 784 1.91e-58 210
MsaG029931 AT5G18960.1 39.837 123 48 4 46 168 35 131 3.65e-17 85.9
MsaG029931 AT5G18960.1 38.532 109 50 2 62 170 210 301 2.74e-15 80.1
MsaG029931 AT3G22170.2 34.219 301 191 2 226 526 381 674 3.48e-58 210
MsaG029931 AT3G22170.2 36.527 167 84 4 24 174 30 190 5.88e-21 98.2
MsaG029931 AT3G22170.1 34.219 301 191 2 226 526 381 674 3.48e-58 210
MsaG029931 AT3G22170.1 36.527 167 84 4 24 174 30 190 5.88e-21 98.2
MsaG029931 AT3G06250.2 37.586 290 174 4 235 523 477 760 8.56e-56 202
MsaG029931 AT3G06250.2 41.667 108 46 3 61 168 26 116 4.54e-17 85.9
MsaG029931 AT3G06250.2 35.172 145 61 4 40 170 155 280 4.54e-16 82.4
MsaG029931 AT3G06250.1 37.586 290 174 4 235 523 477 760 8.56e-56 202
MsaG029931 AT3G06250.1 41.667 108 46 3 61 168 26 116 4.54e-17 85.9
MsaG029931 AT3G06250.1 35.172 145 61 4 40 170 155 280 4.54e-16 82.4
MsaG029931 AT3G06250.3 37.586 290 174 4 235 523 477 760 8.56e-56 202
MsaG029931 AT3G06250.3 41.667 108 46 3 61 168 26 116 4.54e-17 85.9
MsaG029931 AT3G06250.3 35.172 145 61 4 40 170 155 280 4.54e-16 82.4
MsaG029931 AT1G76320.3 34.091 308 184 4 228 524 300 599 7.01e-53 192
MsaG029931 AT1G76320.3 46.087 115 54 4 67 181 1 107 2.39e-19 92.8
MsaG029931 AT1G76320.1 34.091 308 184 4 228 524 300 599 1.06e-52 193
MsaG029931 AT1G76320.1 46.087 115 54 4 67 181 1 107 1.96e-19 93.2
MsaG029931 AT1G76320.4 34.091 308 184 4 228 524 300 599 1.06e-52 193
MsaG029931 AT1G76320.4 46.087 115 54 4 67 181 1 107 1.96e-19 93.2
MsaG029931 AT1G76320.2 34.091 308 184 4 228 524 300 599 1.16e-52 193
MsaG029931 AT1G76320.2 46.087 115 54 4 67 181 1 107 2.06e-19 93.2
MsaG029931 AT4G19990.1 33.775 302 193 4 223 522 260 556 2.37e-48 180
MsaG029931 AT4G19990.3 33.775 302 193 4 223 522 317 613 4.17e-48 180
MsaG029931 AT4G19990.3 36.620 142 69 4 60 188 18 151 2.04e-15 80.5
MsaG029931 AT4G19990.4 33.775 302 193 4 223 522 317 613 4.17e-48 180
MsaG029931 AT4G19990.4 36.620 142 69 4 60 188 18 151 2.04e-15 80.5
MsaG029931 AT4G19990.5 33.775 302 193 4 223 522 317 613 4.30e-48 180
MsaG029931 AT4G19990.5 36.620 142 69 4 60 188 18 151 2.02e-15 80.5
MsaG029931 AT4G19990.7 33.775 302 193 4 223 522 331 627 5.84e-48 180
MsaG029931 AT4G19990.7 36.620 142 69 4 60 188 32 165 1.90e-15 80.5
MsaG029931 AT4G19990.6 33.775 302 193 4 223 522 317 613 6.65e-48 180
MsaG029931 AT4G19990.6 36.620 142 69 4 60 188 18 151 2.73e-15 80.1
MsaG029931 AT4G19990.2 33.775 302 193 4 223 522 317 613 6.65e-48 180
MsaG029931 AT4G19990.2 36.620 142 69 4 60 188 18 151 2.73e-15 80.1
MsaG029931 AT2G32250.2 34.034 238 156 1 226 463 316 552 4.71e-41 160
MsaG029931 AT2G32250.2 38.889 162 91 4 25 186 1 154 8.33e-21 97.8
MsaG029931 AT2G32250.4 34.034 238 156 1 226 463 316 552 4.71e-41 160
MsaG029931 AT2G32250.4 38.889 162 91 4 25 186 1 154 8.33e-21 97.8
MsaG029931 AT2G32250.8 34.034 238 156 1 226 463 275 511 4.88e-41 159
MsaG029931 AT2G32250.8 40.833 120 64 3 67 186 1 113 3.24e-19 92.4
MsaG029931 AT2G32250.7 34.034 238 156 1 226 463 275 511 5.26e-41 159
MsaG029931 AT2G32250.7 40.833 120 64 3 67 186 1 113 3.14e-19 92.4
MsaG029931 AT2G32250.6 34.034 238 156 1 226 463 316 552 5.72e-41 160
MsaG029931 AT2G32250.6 38.889 162 91 4 25 186 1 154 8.49e-21 97.8
MsaG029931 AT2G32250.1 34.034 238 156 1 226 463 316 552 5.72e-41 160
MsaG029931 AT2G32250.1 38.889 162 91 4 25 186 1 154 8.49e-21 97.8
MsaG029931 AT2G32250.3 34.034 238 156 1 226 463 316 552 5.72e-41 160
MsaG029931 AT2G32250.3 38.889 162 91 4 25 186 1 154 8.49e-21 97.8
MsaG029931 AT2G32250.5 34.034 238 156 1 226 463 316 552 6.38e-41 159
MsaG029931 AT2G32250.5 38.889 162 91 4 25 186 1 154 5.81e-21 98.2
MsaG029931 AT1G52520.1 30.278 360 222 12 194 530 349 702 1.29e-36 146
MsaG029931 AT1G52520.1 32.593 135 82 3 35 168 56 182 5.80e-12 69.3
MsaG029931 AT1G80010.1 28.188 298 205 5 238 529 429 723 1.28e-31 131
MsaG029931 AT1G80010.1 35.714 112 56 4 63 170 95 194 1.74e-11 67.8
MsaG029931 AT3G59470.3 43.429 175 78 5 12 170 3 172 2.31e-30 120
MsaG029931 AT3G59470.1 43.429 175 78 5 12 170 3 172 2.31e-30 120
MsaG029931 AT3G59470.2 43.429 175 78 5 12 170 15 184 3.19e-30 120
MsaG029931 AT2G43280.1 45.455 143 68 4 37 177 1 135 2.75e-29 115
MsaG029931 AT3G07500.1 46.667 135 67 3 37 170 6 136 4.80e-28 112
MsaG029931 AT1G10240.2 26.948 308 205 6 234 541 383 670 7.85e-28 119
MsaG029931 AT1G10240.2 26.638 229 157 6 18 241 3 225 4.04e-16 82.4
MsaG029931 AT1G10240.1 26.948 308 205 6 234 541 383 670 7.85e-28 119
MsaG029931 AT1G10240.1 26.638 229 157 6 18 241 3 225 4.04e-16 82.4
MsaG029931 AT2G43280.2 48.039 102 48 3 78 177 2 100 1.70e-21 92.4
MsaG029931 AT5G28530.4 26.780 295 192 8 179 459 269 553 3.17e-21 98.6
MsaG029931 AT5G28530.4 36.364 165 97 5 63 223 55 215 2.52e-17 86.3
MsaG029931 AT5G28530.1 26.780 295 192 8 179 459 332 616 3.95e-21 98.6
MsaG029931 AT5G28530.1 36.364 165 97 5 63 223 55 215 2.74e-17 86.3
MsaG029931 AT5G28530.5 26.780 295 188 9 179 459 269 549 5.56e-21 97.8
MsaG029931 AT5G28530.5 36.364 165 97 5 63 223 55 215 2.57e-17 86.3
MsaG029931 AT5G28530.3 26.780 295 188 9 179 459 332 612 6.36e-21 97.8
MsaG029931 AT5G28530.3 36.364 165 97 5 63 223 55 215 2.52e-17 86.3
MsaG029931 AT5G28530.2 26.780 295 188 9 179 459 332 612 6.36e-21 97.8
MsaG029931 AT5G28530.2 36.364 165 97 5 63 223 55 215 2.52e-17 86.3
MsaG029931 AT4G12850.2 42.982 114 55 3 58 168 3 109 7.09e-21 89.7
MsaG029931 AT4G12850.1 42.982 114 55 3 58 168 3 109 1.22e-20 90.5
MsaG029931 AT4G12850.4 42.982 114 55 3 58 168 3 109 1.55e-20 88.6

Find 146 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTCCCACCGGCAAATTTC+TGG 0.095237 5:-83427516 MsaT029931.1:CDS
ACCTTTATCAACAAATTTAA+AGG 0.150987 5:+83426072 None:intergenic
TCATGTATATACTTACTATT+TGG 0.183092 5:-83426134 MsaT029931.1:CDS
ACATATCATGTAGCTAAATT+TGG 0.219908 5:-83426367 MsaT029931.1:CDS
CCTTCTTTGCAGACATATTC+TGG 0.237940 5:-83426260 MsaT029931.1:CDS
AGAAGAAGAAGATGATGATT+TGG 0.241377 5:-83427937 MsaT029931.1:CDS
TTTGCAACATCATAAGTTTC+AGG 0.247657 5:+83426040 None:intergenic
GCCTTTAAATTTGTTGATAA+AGG 0.252707 5:-83426073 MsaT029931.1:CDS
AACCCTAATTGACACTTTAC+AGG 0.254986 5:-83427484 MsaT029931.1:CDS
TGAAACTCGGCTTCAAAGTT+TGG 0.256048 5:+83426850 None:intergenic
CAGCCTGTAAAGTGTCAATT+AGG 0.264947 5:+83427481 None:intergenic
TTGCAAACTGACGTTGCTTT+TGG 0.278488 5:+83426168 None:intergenic
TAATATCTTGCAGGATGATA+TGG 0.290977 5:-83422551 MsaT029931.1:intron
CACTTTACAGGCTGCTGGTA+TGG 0.294110 5:-83427472 MsaT029931.1:CDS
TCTGCCAAGTGGATCGTATC+TGG 0.294800 5:-83427594 MsaT029931.1:CDS
GTAGAATTTGATATTGGTTT+GGG 0.307344 5:-83428032 MsaT029931.1:CDS
GGTAGAATTTGATATTGGTT+TGG 0.326592 5:-83428033 MsaT029931.1:CDS
CTTAAAGAACTGATTTAAAC+TGG 0.329541 5:+83426603 None:intergenic
GCGTTTGATCTCTCTGTCTT+TGG 0.332960 5:+83427677 None:intergenic
AAGCAATACATTCCGCTTGC+AGG 0.336808 5:-83426732 MsaT029931.1:CDS
TTGACGAATTTGAGTCTTGC+TGG 0.341126 5:-83426801 MsaT029931.1:CDS
CTCCCCATCATCCTCAGCTT+TGG 0.350878 5:+83426396 None:intergenic
GAAGAAGAAGATGATGATTT+GGG 0.351944 5:-83427936 MsaT029931.1:CDS
TACATTCCGCTTGCAGGCAC+TGG 0.358152 5:-83426726 MsaT029931.1:CDS
AGCATGAACTCTTACTTTGA+TGG 0.369267 5:-83426643 MsaT029931.1:CDS
TTGCAGATCCAGAAATTTGC+CGG 0.376547 5:+83427508 None:intergenic
GGAGATGGGGTCCTCAATAC+TGG 0.377437 5:+83426508 None:intergenic
GAAACTCGGCTTCAAAGTTT+GGG 0.402169 5:+83426851 None:intergenic
CGGCTTCAAAGTTTGGGAAC+TGG 0.404355 5:+83426857 None:intergenic
TCTGCACTCATCAAAGAGTA+TGG 0.412149 5:-83427432 MsaT029931.1:CDS
GGTTGGCACGCTAACATTCA+TGG 0.415317 5:-83426422 MsaT029931.1:CDS
AGGTTCCAGATCTCCGGCCT+CGG 0.416546 5:+83427881 None:intergenic
GCTTGCCTCTCCATCGGAGA+TGG 0.422404 5:+83426493 None:intergenic
CTTGCCTCTCCATCGGAGAT+GGG 0.427432 5:+83426494 None:intergenic
TGGTTTGGGAGGAGAATATG+AGG 0.434179 5:-83428018 MsaT029931.1:CDS
ATGAGCTTGAGTGTGCAAAT+AGG 0.437500 5:-83422504 MsaT029931.1:CDS
GGCCTGAAAACTCAAACATT+TGG 0.440126 5:+83426281 None:intergenic
GAGGTGGTAGAATTTGATAT+TGG 0.442215 5:-83428038 MsaT029931.1:CDS
CCATCAGCAATGGGAAGGTT+AGG 0.443830 5:-83425955 MsaT029931.1:CDS
CGAAGGCAGTGAACCTGATC+GGG 0.445748 5:+83427537 None:intergenic
CCAGAATATGTCTGCAAAGA+AGG 0.449302 5:+83426260 None:intergenic
CCAACCCTTGTGACTGTTCG+TGG 0.450668 5:+83427654 None:intergenic
CGAACTTGTTCCACCCGATC+AGG 0.450679 5:-83427550 MsaT029931.1:CDS
TCTGGAACCTTCCGAGGGTA+TGG 0.455551 5:-83427868 MsaT029931.1:CDS
TGAGATGATGGACGAGGATG+AGG 0.456458 5:-83427979 MsaT029931.1:CDS
CACTCGATTCCAAGAGGAGT+TGG 0.457309 5:-83426443 MsaT029931.1:CDS
TTGGGAGAAAGCAGCGGCAG+CGG 0.460216 5:-83427918 MsaT029931.1:CDS
TAATTACACTAGTGGCTGTC+AGG 0.463053 5:-83425903 MsaT029931.1:CDS
AGCCTGTAAAGTGTCAATTA+GGG 0.465989 5:+83427482 None:intergenic
GCGTGCCAACCAACTCCTCT+TGG 0.469006 5:+83426434 None:intergenic
TTGCAACATCATAAGTTTCA+GGG 0.472255 5:+83426041 None:intergenic
GGCTGTCAGGATGAAAGCTT+GGG 0.472435 5:-83425890 MsaT029931.1:CDS
AATGTGTCTCGCAAATATAC+AGG 0.473103 5:+83426700 None:intergenic
GCATCCAAAGCTGAGGATGA+TGG 0.473156 5:-83426400 MsaT029931.1:CDS
TGGCTGTCAGGATGAAAGCT+TGG 0.476969 5:-83425891 MsaT029931.1:CDS
ACTTTACAGGCTGCTGGTAT+GGG 0.477051 5:-83427471 MsaT029931.1:CDS
CATCCAAAGCTGAGGATGAT+GGG 0.487357 5:-83426399 MsaT029931.1:CDS
CCTAACCTTCCCATTGCTGA+TGG 0.492796 5:+83425955 None:intergenic
TATAATGAGATGGAAGAGTA+AGG 0.493011 5:+83426215 None:intergenic
TGAATACTTTGCCAGTATTG+AGG 0.495207 5:-83426519 MsaT029931.1:CDS
TTCACTGCCTTCGTTCCCAC+CGG 0.499535 5:-83427527 MsaT029931.1:CDS
CGAGTCTGAAGAGGCTGCTA+AGG 0.500073 5:-83427841 MsaT029931.1:CDS
ATGATCTCAGGGACAATGAA+TGG 0.501996 5:-83426759 MsaT029931.1:CDS
GGTTCCAGATCTCCGGCCTC+GGG 0.504308 5:+83427882 None:intergenic
AATTCCTATGCTCGTCGTGT+TGG 0.505166 5:-83427810 MsaT029931.1:CDS
GAATTTGATATTGGTTTGGG+AGG 0.506640 5:-83428029 MsaT029931.1:CDS
TAAGCATATCACGAAACTTA+CGG 0.506729 5:-83422527 MsaT029931.1:CDS
CTCATTATATACTCAAACGC+TGG 0.519018 5:-83426201 MsaT029931.1:CDS
GAGACAATTCGTTTGTGCTA+AGG 0.519061 5:-83427730 MsaT029931.1:CDS
AAATGTGAGACAACTTCTCC+TGG 0.528603 5:+83426884 None:intergenic
GGAGAACTCCCATCAGCAAT+GGG 0.531715 5:-83425964 MsaT029931.1:CDS
ATTGACACTTTACAGGCTGC+TGG 0.532057 5:-83427477 MsaT029931.1:CDS
GTTAGAGTTGTCAGTAAAGG+TGG 0.534829 5:-83422422 MsaT029931.1:CDS
CTCGGAAGGTTCCAGATCTC+CGG 0.536526 5:+83427875 None:intergenic
CAATTCGTTTGTGCTAAGGA+AGG 0.537258 5:-83427726 MsaT029931.1:CDS
GAAACCAACACGACGAGCAT+AGG 0.538449 5:+83427806 None:intergenic
ACGAAGGCAGTGAACCTGAT+CGG 0.539597 5:+83427536 None:intergenic
TATGGTGGTATCAGCAAGGT+TGG 0.539841 5:-83427414 MsaT029931.1:CDS
CAAGTTAGAGTTGTCAGTAA+AGG 0.539938 5:-83422425 MsaT029931.1:CDS
CGGAGATCTGGAACCTTCCG+AGG 0.543379 5:-83427874 MsaT029931.1:CDS
TCTCGTCGTTCTCGCCGCGA+CGG 0.544434 5:-83427765 MsaT029931.1:CDS
GGGAGAACTCCCATCAGCAA+TGG 0.547438 5:-83425965 MsaT029931.1:CDS
GCCGCCGCATATTGAGATGA+TGG 0.551096 5:-83427991 MsaT029931.1:CDS
GTTAACACATTTGTGAAACT+CGG 0.558202 5:+83426837 None:intergenic
TCGGATGCTTGCCTCTCCAT+CGG 0.558570 5:+83426487 None:intergenic
AATTCGTTTGTGCTAAGGAA+GGG 0.559501 5:-83427725 MsaT029931.1:CDS
GCGCAGGTAATGCGAAAGGA+GGG 0.559887 5:-83425986 MsaT029931.1:CDS
GATGATGGACGAGGATGAGG+AGG 0.560621 5:-83427976 MsaT029931.1:CDS
GCTATCACTTCGCTGAGTGA+TGG 0.562682 5:+83426663 None:intergenic
CGATTCCAAGAGGAGTTGGT+TGG 0.567024 5:-83426439 MsaT029931.1:CDS
GCATATCTTTAAGCAATGCC+AGG 0.569808 5:-83426902 MsaT029931.1:intron
CTAACCTTCCCATTGCTGAT+GGG 0.576542 5:+83425956 None:intergenic
ATAGGAAGTGTGAAATGTAC+CGG 0.577419 5:-83422486 MsaT029931.1:CDS
AAATTTGCCGGTGGGAACGA+AGG 0.578586 5:+83427520 None:intergenic
TGCACGATTCTTCTGCCAAG+TGG 0.580316 5:-83427605 MsaT029931.1:CDS
ACATTCCGCTTGCAGGCACT+GGG 0.581089 5:-83426725 MsaT029931.1:CDS
ATTGAGGACCCCATCTCCGA+TGG 0.581233 5:-83426503 MsaT029931.1:CDS
CCACGAACAGTCACAAGGGT+TGG 0.582221 5:-83427654 MsaT029931.1:CDS
ATCATATCTGTCTAAAAGTG+TGG 0.585384 5:+83426777 None:intergenic
TGCAACATCATAAGTTTCAG+GGG 0.590648 5:+83426042 None:intergenic
CTTCAAAGTTTGGGAACTGG+AGG 0.598863 5:+83426860 None:intergenic
AGATGGAAGAGTAAGGACAT+TGG 0.600676 5:+83426222 None:intergenic
CGTTTGAGTATATAATGAGA+TGG 0.602082 5:+83426205 None:intergenic
CGCAGGTAATGCGAAAGGAG+GGG 0.602168 5:-83425985 MsaT029931.1:CDS
AACTCCCATCAGCAATGGGA+AGG 0.604290 5:-83425960 MsaT029931.1:CDS
AAACAGAGAGCAAGTTAGAC+CGG 0.606350 5:+83422467 None:intergenic
CAACCCTTGTGACTGTTCGT+GGG 0.608141 5:+83427655 None:intergenic
TGCGCAGGTAATGCGAAAGG+AGG 0.609788 5:-83425987 MsaT029931.1:CDS
TTGCCTCTCCATCGGAGATG+GGG 0.612305 5:+83426495 None:intergenic
CAGATCCAGAAATTTGCCGG+TGG 0.612578 5:+83427511 None:intergenic
TGTGGTTTGGATGGAGAACG+AGG 0.614308 5:-83428057 None:intergenic
AGACTCGAATTCCATACCCT+CGG 0.617359 5:+83427857 None:intergenic
TTTAGACAGATATGATCTCA+GGG 0.617719 5:-83426770 MsaT029931.1:CDS
AACGCCCACGAACAGTCACA+AGG 0.617810 5:-83427659 MsaT029931.1:CDS
AATCATGCTAATTACACTAG+TGG 0.620695 5:-83425911 MsaT029931.1:CDS
ATCCAAAGCTGAGGATGATG+GGG 0.622295 5:-83426398 MsaT029931.1:CDS
AGATCCAGAAATTTGCCGGT+GGG 0.622920 5:+83427512 None:intergenic
TATGGAATTCGAGTCTGAAG+AGG 0.624053 5:-83427850 MsaT029931.1:CDS
CTTGGGAGAGCATATGAATG+AGG 0.624492 5:-83425873 MsaT029931.1:intron
CAAATTTAAAGGCTTCATGT+CGG 0.625446 5:+83426083 None:intergenic
CAGCAAGGTTGGCTTTACTG+AGG 0.625913 5:-83427403 MsaT029931.1:intron
GGACCCCATCTCCGATGGAG+AGG 0.629360 5:-83426498 MsaT029931.1:CDS
ATTAAGATAAGCATGAAAGA+CGG 0.631649 5:-83422394 MsaT029931.1:CDS
AGGCAGTGAACCTGATCGGG+TGG 0.632274 5:+83427540 None:intergenic
AGGTACCCAGTGCCTGCAAG+CGG 0.638903 5:+83426720 None:intergenic
TTTGGGAGGAGAATATGAGG+AGG 0.639911 5:-83428015 MsaT029931.1:CDS
TCGAATTCCATACCCTCGGA+AGG 0.640288 5:+83427861 None:intergenic
CAAAGCTGAGGATGATGGGG+AGG 0.640907 5:-83426395 MsaT029931.1:CDS
GCATATTGAGATGATGGACG+AGG 0.650585 5:-83427985 MsaT029931.1:CDS
TGAAACTTATGATGTTGCAA+AGG 0.650987 5:-83426038 MsaT029931.1:CDS
AGCATGAAAGACGGCCTCTG+AGG 0.655501 5:-83422385 None:intergenic
AGTTGCGCAGGTAATGCGAA+AGG 0.657991 5:-83425990 MsaT029931.1:CDS
TCATGTAGCTAAATTTGGAG+AGG 0.661413 5:-83426362 MsaT029931.1:CDS
GGAGATCTGGAACCTTCCGA+GGG 0.667168 5:-83427873 MsaT029931.1:CDS
ATTCATGGCATCCAAAGCTG+AGG 0.668434 5:-83426407 MsaT029931.1:CDS
GAAGCCAGATACGATCCACT+TGG 0.669193 5:+83427590 None:intergenic
TCGTCCATCATCTCAATATG+CGG 0.670750 5:+83427987 None:intergenic
AGAGTATGGTGGTATCAGCA+AGG 0.675333 5:-83427418 MsaT029931.1:CDS
TCCATCATCTCAATATGCGG+CGG 0.677985 5:+83427990 None:intergenic
GCACTCATCAAAGAGTATGG+TGG 0.683994 5:-83427429 MsaT029931.1:CDS
CAATGAGAAAGAAGTAAGAG+CGG 0.684122 5:-83426554 MsaT029931.1:CDS
GATGATTTGGGAGAAAGCAG+CGG 0.686560 5:-83427924 MsaT029931.1:CDS
ACGCCCACGAACAGTCACAA+GGG 0.715179 5:-83427658 MsaT029931.1:CDS
GGTTTGGATGGAGAACGAGG+TGG 0.749123 5:-83428054 None:intergenic
TCTGAATGATAGCTCCGTCG+CGG 0.754566 5:+83427751 None:intergenic
AGTGCAGACATAATTCTGCG+AGG 0.756220 5:+83427447 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 83422387 83428069 83422387 ID=MsaG029931
Chr5 mRNA 83422387 83428069 83422387 ID=MsaT029931.1;Parent=MsaG029931
Chr5 exon 83422387 83422560 83422387 ID=MsaT029931.1.exon3;Parent=MsaT029931.1
Chr5 CDS 83422387 83422560 83422387 ID=cds.MsaT029931.1;Parent=MsaT029931.1
Chr5 exon 83425874 83426911 83425874 ID=MsaT029931.1.exon2;Parent=MsaT029931.1
Chr5 CDS 83425874 83426911 83425874 ID=cds.MsaT029931.1;Parent=MsaT029931.1
Chr5 exon 83427404 83428069 83427404 ID=MsaT029931.1.exon1;Parent=MsaT029931.1
Chr5 CDS 83427404 83428069 83427404 ID=cds.MsaT029931.1;Parent=MsaT029931.1
Gene Sequence

>MsaT029931.1

ATGGAGAACGAGGTGGTAGAATTTGATATTGGTTTGGGAGGAGAATATGAGGAGGAGCCGCCGCATATTGAGATGATGGACGAGGATGAGGAGGAAGAATATGAAGAAGTAGAAGAAGAAGATGATGATTTGGGAGAAAGCAGCGGCAGCGGTGCTGTTTATTTTCCCGAGGCCGGAGATCTGGAACCTTCCGAGGGTATGGAATTCGAGTCTGAAGAGGCTGCTAAGGCTTTCTATAATTCCTATGCTCGTCGTGTTGGTTTCAGTACTCGAGTCAGTTCTTCTCGTCGTTCTCGCCGCGACGGAGCTATCATTCAGAGACAATTCGTTTGTGCTAAGGAAGGGTTTCGCAATTTGAACGAGAAACGTACCAAAGACAGAGAGATCAAACGCCCACGAACAGTCACAAGGGTTGGATGCAAAGCCTCTTTATCTGTCAAAATGCACGATTCTTCTGCCAAGTGGATCGTATCTGGCTTCGTCAGAGAACACAATCACGAACTTGTTCCACCCGATCAGGTTCACTGCCTTCGTTCCCACCGGCAAATTTCTGGATCTGCAAAAACCCTAATTGACACTTTACAGGCTGCTGGTATGGGTCCTCGCAGAATTATGTCTGCACTCATCAAAGAGTATGGTGGTATCAGCAAGGTTGGCTTTACTGAGCAATGCCAGGAGAAGTTGTCTCACATTTTCCTCCAGTTCCCAAACTTTGAAGCCGAGTTTCACAAATGTGTTAACTTGACTGACTCAATTGACGAATTTGAGTCTTGCTGGTCCACACTTTTAGACAGATATGATCTCAGGGACAATGAATGGCTTCAAGCAATACATTCCGCTTGCAGGCACTGGGTACCTGTATATTTGCGAGACACATTCTTTGCAGAGATGTCCATCACTCAGCGAAGTGATAGCATGAACTCTTACTTTGATGGCTATGTGAATGCTTCAACCAGTTTAAATCAGTTCTTTAAGCTTTATGAGAAAACGCTTGAAAGTCGCAATGAGAAAGAAGTAAGAGCGGATTATGACACAATGAATACTTTGCCAGTATTGAGGACCCCATCTCCGATGGAGAGGCAAGCATCCGAGCTTTACACAAGAAAAATTTTCACTCGATTCCAAGAGGAGTTGGTTGGCACGCTAACATTCATGGCATCCAAAGCTGAGGATGATGGGGAGGTCATTACATATCATGTAGCTAAATTTGGAGAGGATCATAAAGCATTCAATGTTAGATTCAATGTTTTAGAGATGAAAGCGACTTGCAGTTGCCAAATGTTTGAGTTTTCAGGCCTTCTTTGCAGACATATTCTGGCAGTATTTAGAGTTACCAATGTCCTTACTCTTCCATCTCATTATATACTCAAACGCTGGACAAAAAATGCCAAAAGCAACGTCAGTTTGCAAGAACATTCTTCTCATGTATATACTTACTATTTGGAATCTCATACAGTTAGATATAATACTCTCCGACATGAAGCCTTTAAATTTGTTGATAAAGGTGCAAGCTCCCCTGAAACTTATGATGTTGCAAAGGATGCTTTACAAGAAGCTGCAAAAAGAGTTGCGCAGGTAATGCGAAAGGAGGGGAGAACTCCCATCAGCAATGGGAAGGTTAGGAGTCACTTGCTGAATGATGAAAATCATGCTAATTACACTAGTGGCTGTCAGGATGAAAGCTTGGGAGAGCATATGAATGAGGATGATATGGATAAGCATATCACGAAACTTACGGATGAGCTTGAGTGTGCAAATAGGAAGTGTGAAATGTACCGGTCTAACTTGCTCTCTGTTTTGAAAGCTGTTGAGGATCACAAGTTAGAGTTGTCAGTAAAGGTGGAAAACATTAAGATAAGCATGAAAGACGGCCTCTGA
Protein sequence

>MsaT029931.1

MENEVVEFDIGLGGEYEEEPPHIEMMDEDEEEEYEEVEEEDDDLGESSGSGAVYFPEAGDLEPSEGMEFESEEAAKAFYNSYARRVGFSTRVSSSRRSRRDGAIIQRQFVCAKEGFRNLNEKRTKDREIKRPRTVTRVGCKASLSVKMHDSSAKWIVSGFVREHNHELVPPDQVHCLRSHRQISGSAKTLIDTLQAAGMGPRRIMSALIKEYGGISKVGFTEQCQEKLSHIFLQFPNFEAEFHKCVNLTDSIDEFESCWSTLLDRYDLRDNEWLQAIHSACRHWVPVYLRDTFFAEMSITQRSDSMNSYFDGYVNASTSLNQFFKLYEKTLESRNEKEVRADYDTMNTLPVLRTPSPMERQASELYTRKIFTRFQEELVGTLTFMASKAEDDGEVITYHVAKFGEDHKAFNVRFNVLEMKATCSCQMFEFSGLLCRHILAVFRVTNVLTLPSHYILKRWTKNAKSNVSLQEHSSHVYTYYLESHTVRYNTLRHEAFKFVDKGASSPETYDVAKDALQEAAKRVAQVMRKEGRTPISNGKVRSHLLNDENHANYTSGCQDESLGEHMNEDDMDKHITKLTDELECANRKCEMYRSNLLSVLKAVEDHKLELSVKVENIKISMKDGL*