AlfalfaGEDB | MsaG029951

M. sativa ssp. caerulea / MsaG029951

KEGG: K14400
GO: GO:0000993 GO:0001098 GO:0001099 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005849 GO:0006139 GO:0006351 GO:0006353 GO:0006366 GO:0006369 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009909 GO:0009911 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0019899 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0046483 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0070013 GO:0070063 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:2000026 GO:2000241 GO:2000243
PFAM: PF04818.16

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG029951	sp\|Q0WPF2\|PCFS4_ARATH	45.561	856	368	20	1	827	22	808	0.0	629

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG029951	tr\|A0A072U797\|A0A072U797_MEDTR	96.905	840	13	1	1	827	1	840	0.0	1674

TFs/TRs:

Gene ID	Type	Classification
MsaG029951	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000225	MsaG029951	0.817044	7.030881e-52	1.431860e-49
MsaG004039	MsaG029951	0.806125	1.621526e-49	2.523876e-47
MsaG004178	MsaG029951	0.806321	1.475502e-49	2.307341e-47
MsaG004353	MsaG029951	0.822526	3.982810e-53	9.367901e-51
MsaG004517	MsaG029951	0.829518	8.835879e-55	2.522111e-52
MsaG004958	MsaG029951	0.821297	7.647394e-53	1.740640e-50
MsaG005830	MsaG029951	0.802472	9.273954e-49	1.326184e-46
MsaG006046	MsaG029951	0.824280	1.557033e-53	3.840408e-51
MsaG007744	MsaG029951	0.816511	9.245520e-52	1.857133e-49
MsaG008558	MsaG029951	0.811523	1.151128e-50	2.040293e-48
MsaG011082	MsaG029951	0.823708	2.116548e-53	5.140413e-51
MsaG012500	MsaG029951	0.804658	3.281741e-49	4.935860e-47
MsaG016424	MsaG029951	0.851264	1.898219e-60	1.074642e-57
MsaG017173	MsaG029951	0.819202	2.297935e-52	4.948878e-50
MsaG017505	MsaG029951	0.839811	2.339811e-57	9.080254e-55
MsaG018834	MsaG029951	0.807787	7.245311e-50	1.173080e-47
MsaG019288	MsaG029951	0.805265	2.452675e-49	3.741325e-47
MsaG019442	MsaG029951	0.819262	2.226334e-52	4.802342e-50
MsaG019594	MsaG029951	0.815903	1.262596e-51	2.497164e-49
MsaG019925	MsaG029951	0.834419	5.512217e-56	1.814648e-53
MsaG020020	MsaG029951	0.808054	6.361129e-50	1.036545e-47
MsaG021448	MsaG029951	0.829529	8.782452e-55	2.507648e-52
MsaG023762	MsaG029951	0.806696	1.230550e-49	1.941414e-47
MsaG023778	MsaG029951	0.848108	1.430478e-59	7.270335e-57
MsaG027491	MsaG029951	0.818756	2.898828e-52	6.170059e-50
MsaG027708	MsaG029951	0.812325	7.712981e-51	1.394333e-48
MsaG028498	MsaG029951	0.806258	1.520831e-49	2.374701e-47
MsaG029951	MsaG033793	0.844177	1.661018e-58	7.408189e-56
MsaG029951	MsaG036251	0.815696	1.403239e-51	2.760840e-49
MsaG029951	MsaG038016	0.803134	6.780750e-49	9.845034e-47
MsaG029951	MsaG039921	0.822724	3.584869e-53	8.477110e-51
MsaG029951	MsaG041734	0.806588	1.296927e-49	2.040904e-47
MsaG029951	MsaG041905	0.861952	1.412599e-63	1.183383e-60
MsaG029951	MsaG043987	0.801189	1.695800e-48	2.355178e-46
MsaG029951	MsaG044663	0.822667	3.693945e-53	8.721699e-51
MsaG029951	MsaG044664	0.835996	2.213293e-56	7.638308e-54
MsaG029951	MsaG044888	0.877855	9.407230e-69	1.537993e-65
MsaG029951	MsaG046029	0.821226	7.936687e-53	1.803153e-50
MsaG029951	MsaG046770	0.826656	4.288184e-54	1.129340e-51
MsaG029951	MsaG001844	0.837242	1.069535e-56	3.832450e-54
MsaG029951	MsaG004535	0.836634	1.526595e-56	5.370477e-54
MsaG029951	MsaG028289	0.804543	3.466159e-49	5.199544e-47
MsaG029951	MsaG027475	0.821260	7.798607e-53	1.773352e-50
MsaG029951	MsaG034730	0.819722	1.751198e-52	3.822982e-50
MsaG029951	MsaG033321	0.837095	1.165701e-56	4.158513e-54
MsaG029951	MsaG037679	0.806035	1.693889e-49	2.630870e-47
MsaG029951	MsaG036985	0.819977	1.531825e-52	3.366995e-50
MsaG029951	MsaG043457	0.831342	3.179500e-55	9.562884e-53
MsaG029951	MsaG045434	0.804282	3.926776e-49	5.855111e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG029951	MtrunA17_Chr6g0452881	96.905	840	13	1	1	827	1	840	0.0	1674
MsaG029951	MtrunA17_Chr6g0452891	73.469	245	37	3	556	800	1	217	7.22e-120	360
MsaG029951	MtrunA17_Chr4g0037071	89.286	196	21	0	618	813	1	196	1.57e-113	345
MsaG029951	MtrunA17_Chr4g0037081	83.117	154	23	1	296	449	29	179	1.53e-80	256
MsaG029951	MtrunA17_Chr7g0265781	39.031	351	176	9	37	375	78	402	4.47e-61	224
MsaG029951	MtrunA17_Chr7g0265781	37.356	174	105	3	632	803	847	1018	2.46e-33	138
MsaG029951	MtrunA17_Chr1g0179281	38.068	352	189	10	40	381	80	412	1.13e-57	214
MsaG029951	MtrunA17_Chr1g0179281	36.207	174	105	4	632	801	838	1009	2.39e-31	132

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG029951	AT4G04885.1	45.561	856	368	20	1	827	22	808	0.0	629
MsaG029951	AT2G36480.2	36.522	345	182	9	41	366	88	414	7.66e-51	193
MsaG029951	AT2G36480.2	33.140	172	106	3	632	803	785	947	1.75e-25	114
MsaG029951	AT2G36480.1	36.522	345	182	9	41	366	88	414	7.66e-51	193
MsaG029951	AT2G36480.1	33.140	172	106	3	632	803	785	947	1.75e-25	114
MsaG029951	AT1G66500.1	35.326	368	157	16	483	808	68	396	6.36e-50	182
MsaG029951	AT5G43620.1	45.355	183	79	4	635	808	220	390	8.82e-46	170

Find 220 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGATTATGGCCTAGTTAA+AGG	0.199059	6:-286483	MsaT029951.1:CDS
CACATAATGCACATGTATTT+TGG	0.204278	6:+284955	None:intergenic
TCTATTTGTCTTACGTATCT+TGG	0.237116	6:+287191	None:intergenic
CGGCGTCACAATTTACTTAT+AGG	0.255733	6:-285707	MsaT029951.1:CDS
AAGAGTTGTTTCCGATTCTT+TGG	0.278868	6:+285604	None:intergenic
CCCCATCATGCTGTTGATTA+TGG	0.284990	6:-286495	MsaT029951.1:CDS
AGCAGAAGCCCTCTCGTAAA+TGG	0.298225	6:-285109	MsaT029951.1:CDS
TCAGTGTCAAGGGTGTTTCC+TGG	0.301890	6:-286083	MsaT029951.1:CDS
GCTTACTGCCAGAAGTTTCT+TGG	0.305569	6:+286306	None:intergenic
CCTGATGGTTGATTATTAAC+TGG	0.309564	6:+287272	None:intergenic
TAGGCATATGCCCTCATATA+TGG	0.310130	6:-286568	MsaT029951.1:CDS
GCATTGGCTGCAACAGTAAC+AGG	0.313592	6:+286113	None:intergenic
TCTTTGACTGCCTTCTTCAT+AGG	0.318589	6:+284133	None:intergenic
ATGCCTGTTGTTATTCCATT+AGG	0.320007	6:+284858	None:intergenic
GATTGGGATGAGGTGGCATC+TGG	0.331410	6:+285523	None:intergenic
TGAACAATTTCTTCTGTTGA+AGG	0.331657	6:+289827	None:intergenic
GATTCGAATGGATTCCTAAC+AGG	0.333615	6:+285939	None:intergenic
CTGCATTTAGCATGAATAAT+GGG	0.336904	6:+284816	None:intergenic
TGATGAAGAGTTGGCTGTTC+CGG	0.339955	6:-284988	MsaT029951.1:CDS
TCAAAAGCTGCAGGCAAAAC+TGG	0.340119	6:+286032	None:intergenic
TGACGAACCTTCAGAGTATC+TGG	0.351247	6:+285269	None:intergenic
TCTAGGAAGATTACCGTATA+AGG	0.356434	6:+285229	None:intergenic
TCTGCTATGCGACATCTCTT+TGG	0.357635	6:-287362	MsaT029951.1:CDS
TTATTCCATTAGGCGCATTA+AGG	0.358655	6:+284868	None:intergenic
TATGGCCTAGTTAAAGGATT+AGG	0.367937	6:-286477	MsaT029951.1:CDS
TCTGCATTTAGCATGAATAA+TGG	0.369592	6:+284815	None:intergenic
TGCCTATCAAGTCCAATTCT+TGG	0.377024	6:+286585	None:intergenic
CTATTTGTCTTACGTATCTT+GGG	0.379077	6:+287192	None:intergenic
GTTTCATCCTCACAGGTTGC+AGG	0.381654	6:-286714	MsaT029951.1:intron
TGGGATGGAGCGTGTGGGCC+AGG	0.382563	6:+285543	None:intergenic
TACGAGAGGGCTTCTGCTTA+CGG	0.385096	6:+285114	None:intergenic
TAGGCATCAATTAGTGCTCT+TGG	0.388516	6:+286344	None:intergenic
ATGACTCACAATGGTACTAT+TGG	0.398029	6:+285908	None:intergenic
TCTTCGTCACCACCAAGAAT+TGG	0.400029	6:-286597	MsaT029951.1:CDS
GGCTGTTGGCTTGGTACAAA+TGG	0.400747	6:+285495	None:intergenic
GGCATAACAGGTCGTACATT+TGG	0.402384	6:+285642	None:intergenic
CAGCTACCTAATCAGCTTCC+TGG	0.402588	6:-285801	MsaT029951.1:CDS
TCTGCAATCAGTGCCTTATA+CGG	0.402883	6:-285242	MsaT029951.1:CDS
ACGTGGAGCATGCACATTTA+CGG	0.404948	6:+285998	None:intergenic
GCTGATTAGGTAGCTGATAC+AGG	0.407419	6:+285808	None:intergenic
GTGACTGCTGTGCTCGTCTT+GGG	0.407489	6:+285175	None:intergenic
CGATTCTTTGGCCTGGCAAA+GGG	0.408289	6:+285616	None:intergenic
AGAAACCGATAGTTGGTTGC+AGG	0.410939	6:+286147	None:intergenic
CGCATAGCAGAATGTAAACT+TGG	0.411538	6:+287374	None:intergenic
TAAATATTCATCCATATATG+AGG	0.415257	6:+286557	None:intergenic
GACAAAAGCCAAGAAACTTC+TGG	0.416293	6:-286314	MsaT029951.1:CDS
GAAGATCTTCCAACCCTACT+TGG	0.416371	6:+286615	None:intergenic
GGAGCGTGTGGGCCAGGTGG+AGG	0.420614	6:+285549	None:intergenic
GGCAAAGGGAGTGGCATAAC+AGG	0.420754	6:+285630	None:intergenic
GACGGTGTTTGTACACTGTT+TGG	0.421276	6:+285759	None:intergenic
AGAAGTATCGAACATATTCC+TGG	0.424947	6:+287522	None:intergenic
TGTGACTGCTGTGCTCGTCT+TGG	0.427292	6:+285174	None:intergenic
GGATTCTTCGATGGAAATAT+AGG	0.431337	6:+285960	None:intergenic
CTTAGTGGTGCAGAGGCATT+GGG	0.433928	6:-285065	MsaT029951.1:CDS
GACGTGGTTGTCCGTTACTA+AGG	0.434804	6:+286372	None:intergenic
ATCAGACCAGGAAGCTGATT+AGG	0.435569	6:+285795	None:intergenic
GCTTCCTTCACTCTATCTCT+TGG	0.436037	6:-287567	MsaT029951.1:CDS
GTATACCTTAATGCGCCTAA+TGG	0.441302	6:-284873	MsaT029951.1:CDS
CCGATTCTTTGGCCTGGCAA+AGG	0.444884	6:+285615	None:intergenic
CATCAGGCGTTAATTCCCTT+AGG	0.446704	6:-287256	MsaT029951.1:CDS
AATCCTCGTTGGTCGATTCA+AGG	0.447934	6:-289900	MsaT029951.1:CDS
GCCATAATCAACAGCATGAT+GGG	0.449248	6:+286494	None:intergenic
GCTTAGTGGTGCAGAGGCAT+TGG	0.449928	6:-285066	MsaT029951.1:CDS
ACTGTTGGAAATGCAAATTT+CGG	0.451845	6:-286669	MsaT029951.1:CDS
AATCGACCAACGAGGATTGA+TGG	0.453327	6:+289905	None:intergenic
GCTCGTCTTGGGATTTGAAT+CGG	0.457134	6:+285186	None:intergenic
GCCTTTGTTAGTTGAGCGGC+TGG	0.457470	6:-286285	MsaT029951.1:CDS
AGCCAATGAGATTACACCTT+GGG	0.459714	6:+285434	None:intergenic
TGGAAATATAGGAGTTATGC+TGG	0.461860	6:+285971	None:intergenic
TAACAGGTCGTACATTTGGC+AGG	0.462054	6:+285646	None:intergenic
TCTTCCCTCCTTCTGTCTTA+CGG	0.462978	6:-287325	MsaT029951.1:CDS
TTGGGAGCCGAATCAGCTCC+AGG	0.463136	6:-285047	MsaT029951.1:CDS
TTCCATCGAAGAATCCTGTT+AGG	0.463431	6:-285953	MsaT029951.1:CDS
CCAACAGTTGGCTGTTGGCT+TGG	0.468279	6:+285486	None:intergenic
AAAGAAAGATGATGAAGAGT+TGG	0.468658	6:-284997	MsaT029951.1:CDS
GATTGTTCATGCACAGGAAA+AGG	0.472985	6:+285870	None:intergenic
GTTATGCCACTCCCTTTGCC+AGG	0.474289	6:-285627	MsaT029951.1:CDS
GAATCTCCTCGACCAACTCA+TGG	0.475030	6:-287227	MsaT029951.1:CDS
TGTGGGCCAGGTGGAGGTAA+AGG	0.475834	6:+285555	None:intergenic
CTTTGGCCTGGCAAAGGGAG+TGG	0.476898	6:+285621	None:intergenic
CTCTACCTAATCCTTTAACT+AGG	0.477129	6:+286472	None:intergenic
TGGTACAAATGGACTAGGAT+TGG	0.478050	6:+285506	None:intergenic
GGTGAAAGGTTAGACTCAAC+AGG	0.478337	6:-286693	MsaT029951.1:CDS
TTGGCATTTATTGATTCGAA+TGG	0.478491	6:+285927	None:intergenic
GGCCCAAGGTGTAATCTCAT+TGG	0.478710	6:-285436	MsaT029951.1:CDS
TTGAACAATCTCATTGCTCA+TGG	0.479291	6:+289937	None:intergenic
ACTTATAGGCCATCTTTGCC+AGG	0.479835	6:-285693	MsaT029951.1:CDS
CCAGTTAATAATCAACCATC+AGG	0.481608	6:-287272	MsaT029951.1:CDS
ATCAGGCGTTAATTCCCTTA+GGG	0.483589	6:-287255	MsaT029951.1:CDS
GGCATCTGGGATGGAGCGTG+TGG	0.487332	6:+285537	None:intergenic
GGATAGAAATGGTATGGATA+AGG	0.494929	6:-286264	MsaT029951.1:CDS
CAACATCGTAATCATGCTAA+AGG	0.497052	6:-289728	MsaT029951.1:CDS
GCGCCTAATGGAATAACAAC+AGG	0.497466	6:-284861	MsaT029951.1:CDS
TATTTAGCTGACAATTCTAC+TGG	0.498608	6:-286540	MsaT029951.1:CDS
GGCATAGACAGGTCACAGTT+AGG	0.499248	6:-284840	MsaT029951.1:CDS
TATTGTGAAGAATGTTGGCC+AGG	0.499257	6:-287540	MsaT029951.1:CDS
TTAGACTCAACAGGAACTGT+TGG	0.499630	6:-286684	MsaT029951.1:CDS
ATAGTAAGATCAGTGATGAT+AGG	0.500445	6:+289761	None:intergenic
GGTACAAATGGACTAGGATT+GGG	0.500633	6:+285507	None:intergenic
TAAGTAAATTGTGACGCCGC+TGG	0.501369	6:+285711	None:intergenic
GGCCATAATCAACAGCATGA+TGG	0.502882	6:+286493	None:intergenic
ACGGAGGAGTGGATGTATAG+AGG	0.502902	6:-284900	MsaT029951.1:CDS
CTGAAGAGGAGGAGTTCGAT+TGG	0.502985	6:-286217	MsaT029951.1:CDS
CCACAAGTTGTGCACTGTCT+AGG	0.505428	6:+285212	None:intergenic
ATAGATTTCGTTACCTGTGA+AGG	0.506777	6:+285402	None:intergenic
TCACTGTAAAACTCCTCAAA+AGG	0.506831	6:+284927	None:intergenic
GGATGAGGTGGCATCTGGGA+TGG	0.507178	6:+285528	None:intergenic
GGACCATGTCCTGGCAAAGA+TGG	0.508872	6:+285684	None:intergenic
TGCGACATCTCTTTGGTACC+TGG	0.510544	6:-287355	MsaT029951.1:CDS
TAATTTGATTAATTCACTCA+TGG	0.510613	6:-285457	MsaT029951.1:CDS
CACCAAGAATTGGACTTGAT+AGG	0.511974	6:-286587	MsaT029951.1:CDS
TCTGATGAGAAACCGATAGT+TGG	0.513144	6:+286140	None:intergenic
TAATCAATTTGTCCCAAGTA+GGG	0.514638	6:-286628	MsaT029951.1:CDS
CCTTTGCCAGGCCAAAGAAT+CGG	0.515014	6:-285615	MsaT029951.1:CDS
CAATTTGTCCCAAGTAGGGT+TGG	0.520932	6:-286624	MsaT029951.1:CDS
AGGCCATCTTTGCCAGGACA+TGG	0.522510	6:-285687	MsaT029951.1:CDS
TTGCGACCTTTACCTCCACC+TGG	0.523511	6:-285561	MsaT029951.1:CDS
GGAGTTATGCTGGGAGGACG+TGG	0.523802	6:+285981	None:intergenic
TTGTTTCCGATTCTTTGGCC+TGG	0.524049	6:+285609	None:intergenic
GTGTTTGTACACTGTTTGGC+GGG	0.527616	6:+285763	None:intergenic
AGAGCACTAATTGATGCCTA+TGG	0.528940	6:-286341	MsaT029951.1:CDS
TGCCTCATTTCAAAAGCTGC+AGG	0.529200	6:+286023	None:intergenic
CAAAATACATGTGCATTATG+TGG	0.529245	6:-284954	MsaT029951.1:CDS
GTTGAGCGGCTGGATAGAAA+TGG	0.532637	6:-286275	MsaT029951.1:CDS
AAGGTCGCAAAGCTCCTCCC+TGG	0.535607	6:+285574	None:intergenic
TGAAGAGGAGGAGTTCGATT+GGG	0.536389	6:-286216	MsaT029951.1:CDS
TAGATTTCGTTACCTGTGAA+GGG	0.539547	6:+285403	None:intergenic
GAATAACAACAGGCATAGAC+AGG	0.540275	6:-284851	MsaT029951.1:CDS
GGTTTCCTGCAACCAACTAT+CGG	0.543300	6:-286152	MsaT029951.1:CDS
CCATCACAAAATCTTCAGAC+GGG	0.543390	6:+284781	None:intergenic
GAGGAGATTCAGAAGCCCTA+AGG	0.544074	6:+287240	None:intergenic
AGCACAGCAGTCACATGGAT+TGG	0.544101	6:-285166	MsaT029951.1:CDS
AATATCCAACAGTTGGCTGT+TGG	0.544187	6:+285481	None:intergenic
AAATTAGAATATCCAACAGT+TGG	0.545058	6:+285474	None:intergenic
GGTGTTTGTACACTGTTTGG+CGG	0.545314	6:+285762	None:intergenic
TTAATCAATTTGTCCCAAGT+AGG	0.545867	6:-286629	MsaT029951.1:CDS
CTCATAGCAGGACCATGTCC+TGG	0.548016	6:+285675	None:intergenic
GACAGTATTGTGAAGAATGT+TGG	0.548430	6:-287545	MsaT029951.1:CDS
TGACACTGAAGAAGTTGCAT+TGG	0.548483	6:+286097	None:intergenic
TGGCTTGGTACAAATGGACT+AGG	0.548518	6:+285501	None:intergenic
GGCTAATCAAGCCCCTTCAC+AGG	0.551075	6:-285415	MsaT029951.1:intron
GCGGCTGGATAGAAATGGTA+TGG	0.551376	6:-286270	MsaT029951.1:CDS
GTAGACCATGGTAGAAGTAA+TGG	0.552062	6:-286173	MsaT029951.1:CDS
CCTCACAGGTTGCAGGTGAA+AGG	0.553428	6:-286707	MsaT029951.1:intron
ATGCAACTTCTTCAGTGTCA+AGG	0.556856	6:-286094	MsaT029951.1:CDS
CTACCATGGTCTACTACTGC+AGG	0.557738	6:+286182	None:intergenic
GCATCTGGGATGGAGCGTGT+GGG	0.559981	6:+285538	None:intergenic
ATCTTCCGTAAGACAGAAGG+AGG	0.562765	6:+287320	None:intergenic
TTCCTAACAGGATTCTTCGA+TGG	0.563320	6:+285951	None:intergenic
CTAAGGGAATTAACGCCTGA+TGG	0.563337	6:+287257	None:intergenic
ATTGGGATGAGGTGGCATCT+GGG	0.564645	6:+285524	None:intergenic
TGCAACTTCTTCAGTGTCAA+GGG	0.565136	6:-286093	MsaT029951.1:CDS
CCAAGCCAACAGCCAACTGT+TGG	0.566173	6:-285486	MsaT029951.1:CDS
TATTTGCAACCGTATTGTTG+AGG	0.569428	6:-289693	MsaT029951.1:intron
ATGCAGGGCACCTATGAAGA+AGG	0.570350	6:-284143	MsaT029951.1:intron
CTATCAAGTCCAATTCTTGG+TGG	0.571361	6:+286588	None:intergenic
TCCATCACAAAATCTTCAGA+CGG	0.571706	6:+284780	None:intergenic
TCAATATTTGAATTGAGACC+AGG	0.575901	6:+286065	None:intergenic
ATTAATTCACTCATGGCCCA+AGG	0.576294	6:-285450	MsaT029951.1:CDS
AAGATCTTCCAACCCTACTT+GGG	0.576601	6:+286616	None:intergenic
GTAAGCCTTTGTTAGTTGAG+CGG	0.577243	6:-286289	MsaT029951.1:CDS
AACAGGTCGTACATTTGGCA+GGG	0.583155	6:+285647	None:intergenic
TAGCTGACAATTCTACTGGT+AGG	0.585559	6:-286536	MsaT029951.1:CDS
CCTAGACAGTGCACAACTTG+TGG	0.586792	6:-285212	MsaT029951.1:CDS
AATAGTACCATTGTGAGTCA+TGG	0.588735	6:-285906	MsaT029951.1:CDS
ACAGGTCGTACATTTGGCAG+GGG	0.590048	6:+285648	None:intergenic
GATGGAGCGTGTGGGCCAGG+TGG	0.590396	6:+285546	None:intergenic
TGCAACGATTGTTCATGCAC+AGG	0.594090	6:+285864	None:intergenic
GGACTAGGATTGGGATGAGG+TGG	0.594447	6:+285516	None:intergenic
AGACGAGCACAGCAGTCACA+TGG	0.599845	6:-285171	MsaT029951.1:CDS
AGATTTCGTTACCTGTGAAG+GGG	0.600998	6:+285404	None:intergenic
AGTGAGACGATGTGGCTTAG+TGG	0.601641	6:-285080	MsaT029951.1:CDS
AATCTCCTCGACCAACTCAT+GGG	0.602277	6:-287226	MsaT029951.1:CDS
GTTTGATCCAGATACTCTGA+AGG	0.602293	6:-285276	MsaT029951.1:CDS
AGGAGATTCAGAAGCCCTAA+GGG	0.603089	6:+287241	None:intergenic
TCAATCTTCCGTAAGACAGA+AGG	0.606789	6:+287317	None:intergenic
CTCACAAACCATTTACGAGA+GGG	0.607148	6:+285101	None:intergenic
GGAAACCATTACTTCTACCA+TGG	0.608961	6:+286168	None:intergenic
TCCAGCCGCTCAACTAACAA+AGG	0.610598	6:+286284	None:intergenic
GGGTTAGATGAAATCAGACC+AGG	0.611439	6:+285783	None:intergenic
GGAAATATAGGAGTTATGCT+GGG	0.611878	6:+285972	None:intergenic
TTAACATGTATCCCATGAGT+TGG	0.613297	6:+287215	None:intergenic
AACGTCGTGAAGAAGATCCT+CGG	0.613895	6:-289865	MsaT029951.1:CDS
CCTCCACCATCAATCCTCGT+TGG	0.618578	6:-289911	MsaT029951.1:CDS
TGTATCCCATGAGTTGGTCG+AGG	0.619165	6:+287221	None:intergenic
AAAGCTGCAGGCAAAACTGG+TGG	0.620190	6:+286035	None:intergenic
AAATATTCATCCATATATGA+GGG	0.620513	6:+286558	None:intergenic
CGACCAACGAGGATTGATGG+TGG	0.625674	6:+289908	None:intergenic
TGTGACGCCGCTGGATCCTG+AGG	0.629147	6:+285720	None:intergenic
CCAACGAGGATTGATGGTGG+AGG	0.631737	6:+289911	None:intergenic
ATGGCAGAATACTGAAGAGG+AGG	0.632607	6:-286228	MsaT029951.1:CDS
TGCATTTAGCATGAATAATG+GGG	0.635399	6:+284817	None:intergenic
ACAGTGATGAAACGGAGGAG+TGG	0.635697	6:-284911	MsaT029951.1:CDS
CGTCTTGGGATTTGAATCGG+AGG	0.638892	6:+285189	None:intergenic
CTGTCCAAGAGATAGAGTGA+AGG	0.641585	6:+287563	None:intergenic
AATGGACTAGGATTGGGATG+AGG	0.642770	6:+285513	None:intergenic
GTTGGCTGTTCCGGCCGACG+AGG	0.646045	6:-284979	MsaT029951.1:CDS
TGTCTTACGGAAGATTGACG+TGG	0.654293	6:-287312	MsaT029951.1:CDS
ATAAGGTTATGACAACATCA+TGG	0.656509	6:-286247	MsaT029951.1:CDS
AATTAGACCATGACTCACAA+TGG	0.658655	6:+285899	None:intergenic
CTGCAGGCAAAACTGGTGGA+CGG	0.660560	6:+286039	None:intergenic
ATGACATATAGCCTTAGTAA+CGG	0.660592	6:-286383	MsaT029951.1:CDS
TAGCCAATGAGATTACACCT+TGG	0.661273	6:+285433	None:intergenic
GATGTGGCTTAGTGGTGCAG+AGG	0.663497	6:-285072	MsaT029951.1:CDS
ATCATGGCAGAATACTGAAG+AGG	0.665757	6:-286231	MsaT029951.1:CDS
TAACCTACTTCGGGTAGACG+CGG	0.669168	6:+287497	None:intergenic
CATCACAAAATCTTCAGACG+GGG	0.674739	6:+284782	None:intergenic
CCATAATCAACAGCATGATG+GGG	0.676024	6:+286495	None:intergenic
CAGGTCGTACATTTGGCAGG+GGG	0.677425	6:+285649	None:intergenic
ACTCACAAACCATTTACGAG+AGG	0.681960	6:+285100	None:intergenic
GACGCCGCTGGATCCTGAGG+TGG	0.683714	6:+285723	None:intergenic
GTGCATGAACAATCGTTGCA+CGG	0.685001	6:-285861	MsaT029951.1:CDS
AGTCCTGCAGTAGTAGACCA+TGG	0.689778	6:-286185	MsaT029951.1:CDS
GGTTGCGAATTTCTCAACCG+AGG	0.700396	6:+289848	None:intergenic
GCAGTCAAAGAAAACGAATG+CGG	0.700511	6:-284121	MsaT029951.1:CDS
GGTTTGTGAGTGAGACGATG+TGG	0.720083	6:-285088	MsaT029951.1:CDS
TCTTCCGTAAGACAGAAGGA+GGG	0.728444	6:+287321	None:intergenic
AATATAGGAGTTATGCTGGG+AGG	0.735215	6:+285975	None:intergenic
TTTGCCAACTGAGACCACTG+AGG	0.763418	6:-285021	MsaT029951.1:CDS
AAGCCTTGAATCGACCAACG+AGG	0.765396	6:+289897	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	284115	289957	284115	ID=MsaG029951
Chr6	mRNA	284115	289957	284115	ID=MsaT029951.1;Parent=MsaG029951
Chr6	exon	284115	284159	284115	ID=MsaT029951.1.exon7;Parent=MsaT029951.1
Chr6	CDS	284115	284159	284115	ID=cds.MsaT029951.1;Parent=MsaT029951.1
Chr6	exon	284773	285315	284773	ID=MsaT029951.1.exon6;Parent=MsaT029951.1
Chr6	CDS	284773	285315	284773	ID=cds.MsaT029951.1;Parent=MsaT029951.1
Chr6	exon	285416	286427	285416	ID=MsaT029951.1.exon5;Parent=MsaT029951.1
Chr6	CDS	285416	286427	285416	ID=cds.MsaT029951.1;Parent=MsaT029951.1
Chr6	exon	286465	286721	286465	ID=MsaT029951.1.exon4;Parent=MsaT029951.1
Chr6	CDS	286465	286721	286465	ID=cds.MsaT029951.1;Parent=MsaT029951.1
Chr6	exon	287166	287426	287166	ID=MsaT029951.1.exon3;Parent=MsaT029951.1
Chr6	CDS	287166	287426	287166	ID=cds.MsaT029951.1;Parent=MsaT029951.1
Chr6	exon	287505	287606	287505	ID=MsaT029951.1.exon2;Parent=MsaT029951.1
Chr6	CDS	287505	287606	287505	ID=cds.MsaT029951.1;Parent=MsaT029951.1
Chr6	exon	289694	289957	289694	ID=MsaT029951.1.exon1;Parent=MsaT029951.1
Chr6	CDS	289694	289957	289694	ID=cds.MsaT029951.1;Parent=MsaT029951.1

Gene Sequence

>MsaT029951.1

ATGAGCAATGAGATTGTTCAAAAGCCTCCACCATCAATCCTCGTTGGTCGATTCAAGGCTTTGTTGAATCAACGTCGTGAAGAAGATCCTCGGTTGAGAAATTCGCAACCTTCAACAGAAGAAATTGTTCAGATTTACGAGCTTTTGTTGTCTGAACTCACGTGTAATGTTAAACCTATCATCACTGATCTTACTATCATTGCTGAACAACATCGTAATCATGCTAAAGGAATCGCTTTTGCTATTTGCAACCGTATTGTTGAGGTGTCTGCAGATCAAAAGCTTCCTTCACTCTATCTCTTGGACAGTATTGTGAAGAATGTTGGCCAGGAATATGTTCGATACTTCTCACCGCGTCTACCCGAAGTTTTCTGCGTGGCATACAGACAAGTTGATCCAAGTTTACATTCTGCTATGCGACATCTCTTTGGTACCTGGTCAAAAGTCTTCCCTCCTTCTGTCTTACGGAAGATTGACGTGGAATTGCAATTTTCTAAACCAGTTAATAATCAACCATCAGGCGTTAATTCCCTTAGGGCTTCTGAATCTCCTCGACCAACTCATGGGATACATGTTAACCCAAGATACGTAAGACAAATAGAACACTCTACTTCAGTTATTGATAGTGTTGCAGGTGAAAGGTTAGACTCAACAGGAACTGTTGGAAATGCAAATTTCGGTCTTCTTGCTAGTAAGATTAATCAATTTGTCCCAAGTAGGGTTGGAAGATCTTCGTCACCACCAAGAATTGGACTTGATAGGCATATGCCCTCATATATGGATGAATATTTAGCTGACAATTCTACTGGTAGGACAGTTGAGAGAGAATCTCCCCATCATGCTGTTGATTATGGCCTAGTTAAAGGATTAGGTAGAGAAGAAGATCGGAAGAGATTTCAAACTTCTATGACATATAGCCTTAGTAACGGACAACCACGTCAAAATCCAAGAGCACTAATTGATGCCTATGGTAGTGACAAAAGCCAAGAAACTTCTGGCAGTAAGCCTTTGTTAGTTGAGCGGCTGGATAGAAATGGTATGGATAAGGTTATGACAACATCATGGCAGAATACTGAAGAGGAGGAGTTCGATTGGGAAGATATGAGTCCTGCAGTAGTAGACCATGGTAGAAGTAATGGTTTCCTGCAACCAACTATCGGTTTCTCATCAGAAAAGCCTGTTACTGTTGCAGCCAATGCAACTTCTTCAGTGTCAAGGGTGTTTCCTGGTCTCAATTCAAATATTGAGTACCGTCCACCAGTTTTGCCTGCAGCTTTTGAAATGAGGCACTCCGTAAATGTGCATGCTCCACGTCCTCCCAGCATAACTCCTATATTTCCATCGAAGAATCCTGTTAGGAATCCATTCGAATCAATAAATGCCAATAGTACCATTGTGAGTCATGGTCTAATTAATAGACCTTTTCCTGTGCATGAACAATCGTTGCACGGTGTTGAGAACAATGATATCAATAAAAGAAACCTGTATCAGCTACCTAATCAGCTTCCTGGTCTGATTTCATCTAACCCGCCAAACAGTGTACAAACACCGTCATTCCAGTTTTTTCCACCTCAGGATCCAGCGGCGTCACAATTTACTTATAGGCCATCTTTGCCAGGACATGGTCCTGCTATGAGTAACCCCCTGCCAAATGTACGACCTGTTATGCCACTCCCTTTGCCAGGCCAAAGAATCGGAAACAACTCTTTTCATTTCCAGGGAGGAGCTTTGCGACCTTTACCTCCACCTGGCCCACACGCTCCATCCCAGATGCCACCTCATCCCAATCCTAGTCCATTTGTACCAAGCCAACAGCCAACTGTTGGATATTCTAATTTGATTAATTCACTCATGGCCCAAGGTGTAATCTCATTGGCTAATCAAGCCCCTTCACAGGATTTTGTTGGCATTGAGTTTGATCCAGATACTCTGAAGGTTCGTCATGAATCTGCAATCAGTGCCTTATACGGTAATCTTCCTAGACAGTGCACAACTTGTGGCCTCCGATTCAAATCCCAAGACGAGCACAGCAGTCACATGGATTGGCATGTGACTAAGAACCGTATGTCTAAAAACCGTAAGCAGAAGCCCTCTCGTAAATGGTTTGTGAGTGAGACGATGTGGCTTAGTGGTGCAGAGGCATTGGGAGCCGAATCAGCTCCAGGTTTTTTGCCAACTGAGACCACTGAGGAAAAGAAAGATGATGAAGAGTTGGCTGTTCCGGCCGACGAGGACCAAAATACATGTGCATTATGTGGAGAACCTTTTGAGGAGTTTTACAGTGATGAAACGGAGGAGTGGATGTATAGAGGAGCTGTATACCTTAATGCGCCTAATGGAATAACAACAGGCATAGACAGGTCACAGTTAGGCCCCATTATTCATGCTAAATGCAGATCTGAATCTACCCCGTCTGAAGATTTTGTGATGGACGAAGGGGGCACCTATGAAGAAGGCAGTCAAAGAAAACGAATGCGGAGCTGA

Protein sequence

>MsaT029951.1

MSNEIVQKPPPSILVGRFKALLNQRREEDPRLRNSQPSTEEIVQIYELLLSELTCNVKPIITDLTIIAEQHRNHAKGIAFAICNRIVEVSADQKLPSLYLLDSIVKNVGQEYVRYFSPRLPEVFCVAYRQVDPSLHSAMRHLFGTWSKVFPPSVLRKIDVELQFSKPVNNQPSGVNSLRASESPRPTHGIHVNPRYVRQIEHSTSVIDSVAGERLDSTGTVGNANFGLLASKINQFVPSRVGRSSSPPRIGLDRHMPSYMDEYLADNSTGRTVERESPHHAVDYGLVKGLGREEDRKRFQTSMTYSLSNGQPRQNPRALIDAYGSDKSQETSGSKPLLVERLDRNGMDKVMTTSWQNTEEEEFDWEDMSPAVVDHGRSNGFLQPTIGFSSEKPVTVAANATSSVSRVFPGLNSNIEYRPPVLPAAFEMRHSVNVHAPRPPSITPIFPSKNPVRNPFESINANSTIVSHGLINRPFPVHEQSLHGVENNDINKRNLYQLPNQLPGLISSNPPNSVQTPSFQFFPPQDPAASQFTYRPSLPGHGPAMSNPLPNVRPVMPLPLPGQRIGNNSFHFQGGALRPLPPPGPHAPSQMPPHPNPSPFVPSQQPTVGYSNLINSLMAQGVISLANQAPSQDFVGIEFDPDTLKVRHESAISALYGNLPRQCTTCGLRFKSQDEHSSHMDWHVTKNRMSKNRKQKPSRKWFVSETMWLSGAEALGAESAPGFLPTETTEEKKDDEELAVPADEDQNTCALCGEPFEEFYSDETEEWMYRGAVYLNAPNGITTGIDRSQLGPIIHAKCRSESTPSEDFVMDEGGTYEEGSQRKRMRS*