Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG030050 | XP_003607052.1 | 98.013 | 151 | 3 | 0 | 1 | 151 | 1 | 151 | 1.02e-105 | 308 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG030050 | sp|F4JZT3|ARR24_ARATH | 32.143 | 112 | 76 | 0 | 33 | 144 | 18 | 129 | 1.38e-16 | 74.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG030050 | tr|G7JJU6|G7JJU6_MEDTR | 98.013 | 151 | 3 | 0 | 1 | 151 | 1 | 151 | 4.89e-106 | 308 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG030050 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG030050 | MtrunA17_Chr6g0451941 | 98.013 | 151 | 3 | 0 | 1 | 151 | 1 | 151 | 9.42e-110 | 308 |
| MsaG030050 | MtrunA17_Chr7g0262351 | 34.513 | 113 | 73 | 1 | 25 | 137 | 14 | 125 | 8.24e-18 | 74.7 |
| MsaG030050 | MtrunA17_Chr8g0364781 | 35.185 | 108 | 68 | 1 | 33 | 138 | 12 | 119 | 1.80e-15 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG030050 | AT5G26594.1 | 32.143 | 112 | 76 | 0 | 33 | 144 | 18 | 129 | 1.41e-17 | 74.7 |
Find 29 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTCTCGGGTCAAAGGTTT+TGG | 0.171590 | 6:+1810550 | None:intergenic |
| TACCTCGGGTCCATTGATTA+TGG | 0.250485 | 6:+1811846 | None:intergenic |
| AGCAATGCATTCATTGGATA+TGG | 0.328332 | 6:+1812021 | None:intergenic |
| GATCAAAACGTGCAAATGTT+TGG | 0.372265 | 6:-1810598 | MsaT030050.1:CDS |
| CATCATATGCTTGAATCAAT+AGG | 0.390164 | 6:-1811964 | MsaT030050.1:CDS |
| TTTGATTTGCCCATAATCAA+TGG | 0.409566 | 6:-1811856 | MsaT030050.1:CDS |
| CACTATGTGCAGGCTCTGTT+CGG | 0.424190 | 6:+1811938 | None:intergenic |
| ACCTCGGGTCCATTGATTAT+GGG | 0.437317 | 6:+1811847 | None:intergenic |
| TCTACTAGCAATGCATTCAT+TGG | 0.442738 | 6:+1812015 | None:intergenic |
| GTGAGGCAGATGCGTGCCAT+GGG | 0.476591 | 6:-1810661 | MsaT030050.1:CDS |
| CATATGATGCTCTAAGCGTC+TGG | 0.510351 | 6:+1811978 | None:intergenic |
| TGATGAACACAAAACTCTCT+CGG | 0.519844 | 6:+1810536 | None:intergenic |
| ACAAAACTCTCTCGGGTCAA+AGG | 0.520328 | 6:+1810544 | None:intergenic |
| ATGAATGCATTGCTAGTAGA+TGG | 0.520529 | 6:-1812012 | MsaT030050.1:CDS |
| GATGAACACAAAACTCTCTC+GGG | 0.532140 | 6:+1810537 | None:intergenic |
| GCTTCTTGAGCACTATGTGC+AGG | 0.556886 | 6:+1811928 | None:intergenic |
| AAGTTGCAATCAACAATGGA+TGG | 0.566106 | 6:-1812123 | None:intergenic |
| TGCCATGGGGATACAGAGCA+AGG | 0.576127 | 6:-1810647 | MsaT030050.1:CDS |
| GGTGAGGCAGATGCGTGCCA+TGG | 0.582675 | 6:-1810662 | MsaT030050.1:CDS |
| GGGATACAGAGCAAGGTGGT+TGG | 0.588650 | 6:-1810640 | MsaT030050.1:CDS |
| CACCTTGCTCTGTATCCCCA+TGG | 0.599686 | 6:+1810645 | None:intergenic |
| AACAATGGATGGAGGAACCA+CGG | 0.600766 | 6:-1812112 | None:intergenic |
| ATCTGCATTAGTTTCACATG+CGG | 0.612322 | 6:+1810500 | None:intergenic |
| ACAATGGATGGAGGAACCAC+GGG | 0.635016 | 6:-1812111 | MsaT030050.1:CDS |
| GCCCATAATCAATGGACCCG+AGG | 0.655096 | 6:-1811848 | MsaT030050.1:intron |
| GAGGAACCACGGGATATAAG+CGG | 0.658571 | 6:-1812101 | MsaT030050.1:CDS |
| TTGCAATCAACAATGGATGG+AGG | 0.668830 | 6:-1812120 | None:intergenic |
| CATGGGGATACAGAGCAAGG+TGG | 0.752735 | 6:-1810644 | MsaT030050.1:CDS |
| TGAGGCAGATGCGTGCCATG+GGG | 0.759926 | 6:-1810660 | MsaT030050.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 1810513 | 1812130 | 1810513 | ID=MsaG030050 |
| Chr6 | mRNA | 1810513 | 1812130 | 1810513 | ID=MsaT030050.1;Parent=MsaG030050 |
| Chr6 | exon | 1810513 | 1810686 | 1810513 | ID=MsaT030050.1.exon2;Parent=MsaT030050.1 |
| Chr6 | CDS | 1810513 | 1810686 | 1810513 | ID=cds.MsaT030050.1;Parent=MsaT030050.1 |
| Chr6 | exon | 1811849 | 1812130 | 1811849 | ID=MsaT030050.1.exon1;Parent=MsaT030050.1 |
| Chr6 | CDS | 1811849 | 1812130 | 1811849 | ID=cds.MsaT030050.1;Parent=MsaT030050.1 |
| Gene Sequence |
| Protein sequence |
