AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG030060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030060 MtrunA17_Chr6g0451711 92.216 501 37 1 1 499 1 501 0.0 959
MsaG030060 MtrunA17_Chr5g0425371 69.920 502 143 4 1 499 1 497 0.0 728
MsaG030060 MtrunA17_Chr7g0236991 69.261 501 148 3 1 499 1 497 0.0 727
MsaG030060 MtrunA17_Chr7g0237001 69.062 501 149 3 1 499 1 497 0.0 725
MsaG030060 MtrunA17_Chr7g0237041 69.062 501 149 3 1 499 1 497 0.0 725
MsaG030060 MtrunA17_Chr7g0237081 69.062 501 149 3 1 499 1 497 0.0 725
MsaG030060 MtrunA17_Chr7g0237051 69.062 501 149 3 1 499 1 497 0.0 725
MsaG030060 MtrunA17_Chr6g0465911 69.610 487 143 3 16 499 13 497 0.0 717
MsaG030060 MtrunA17_Chr6g0466051 69.405 487 144 3 16 499 13 497 0.0 714
MsaG030060 MtrunA17_Chr6g0465441 67.901 486 153 2 16 499 13 497 0.0 711
MsaG030060 MtrunA17_Chr3g0105071 60.958 543 197 5 1 529 1 542 0.0 683
MsaG030060 MtrunA17_Chr4g0024691 65.121 496 170 2 6 499 11 505 0.0 678
MsaG030060 MtrunA17_Chr6g0465851 69.397 464 137 3 39 499 1 462 0.0 675
MsaG030060 MtrunA17_Chr7g0236961 70.227 440 129 1 62 499 3 442 0.0 654
MsaG030060 MtrunA17_Chr7g0237011 70.000 440 130 1 62 499 3 442 0.0 651
MsaG030060 MtrunA17_Chr3g0082691 63.265 490 176 3 11 497 7 495 0.0 649
MsaG030060 MtrunA17_Chr5g0431571 62.781 489 178 3 12 497 4 491 0.0 637
MsaG030060 MtrunA17_Chr5g0431651 60.816 490 189 2 11 497 5 494 0.0 630
MsaG030060 MtrunA17_Chr4g0042111 54.268 492 219 4 10 497 15 504 0.0 558
MsaG030060 MtrunA17_Chr6g0465981 71.823 362 101 1 16 377 13 373 0.0 550
MsaG030060 MtrunA17_Chr6g0465871 71.823 362 101 1 16 377 13 373 0.0 550
MsaG030060 MtrunA17_Chr6g0466081 71.823 362 101 1 16 377 13 373 0.0 550
MsaG030060 MtrunA17_Chr6g0466111 71.681 339 95 1 39 377 1 338 2.27e-175 508
MsaG030060 MtrunA17_Chr6g0465931 71.386 339 96 1 39 377 1 338 2.14e-174 505
MsaG030060 MtrunA17_Chr7g0236971 68.406 345 107 1 157 499 1 345 3.87e-170 497
MsaG030060 MtrunA17_Chr7g0236951 73.418 316 84 0 62 377 3 318 8.79e-170 493
MsaG030060 MtrunA17_Chr7g0237031 73.101 316 85 0 62 377 3 318 6.81e-169 490
MsaG030060 MtrunA17_Chr8g0336181 45.418 491 259 6 13 496 9 497 1.15e-149 450
MsaG030060 MtrunA17_Chr2g0319041 43.561 528 270 10 11 515 8 530 2.31e-146 442
MsaG030060 MtrunA17_Chr2g0319051 44.273 454 248 5 48 497 4 456 7.82e-133 405
MsaG030060 MtrunA17_Chr4g0008621 46.447 394 206 5 14 403 18 410 4.11e-124 380
MsaG030060 MtrunA17_Chr5g0445511 63.866 238 86 0 153 390 2 239 2.84e-106 326
MsaG030060 MtrunA17_Chr7g0237071 75.000 200 50 0 62 261 3 202 1.14e-103 318
MsaG030060 MtrunA17_Chr4g0023911 35.271 499 303 6 16 505 44 531 2.31e-94 306
MsaG030060 MtrunA17_Chr6g0466021 72.632 190 51 1 16 205 13 201 3.44e-93 290
MsaG030060 MtrunA17_Chr2g0310331 35.800 500 309 6 2 497 38 529 1.16e-92 303
MsaG030060 MtrunA17_Chr2g0323131 37.055 421 249 4 68 482 84 494 7.12e-90 294
MsaG030060 MtrunA17_Chr2g0323151 37.046 413 251 3 72 482 4 409 4.16e-89 290
MsaG030060 MtrunA17_Chr2g0319071 36.188 467 247 11 43 504 18 438 9.64e-88 286
MsaG030060 MtrunA17_Chr8g0357411 35.354 495 307 7 8 497 46 532 7.58e-87 287
MsaG030060 MtrunA17_Chr6g0466031 62.745 204 72 2 299 499 2 204 1.94e-84 270
MsaG030060 MtrunA17_Chr4g0048911 29.712 451 307 6 55 499 61 507 8.21e-67 232
MsaG030060 MtrunA17_Chr2g0310401 34.677 372 237 3 131 497 5 375 2.01e-66 227
MsaG030060 MtrunA17_Chr6g0460371 95.536 112 4 1 627 738 110 220 2.78e-64 229
MsaG030060 MtrunA17_Chr4g0048921 29.842 506 326 10 43 528 63 559 4.21e-64 225
MsaG030060 MtrunA17_Chr7g0237061 61.728 162 60 1 299 458 2 163 6.53e-61 206
MsaG030060 MtrunA17_Chr7g0236981 66.912 136 41 2 1 136 1 132 7.82e-59 196
MsaG030060 MtrunA17_Chr2g0280581 26.424 439 313 4 62 493 23 458 8.77e-54 195
MsaG030060 MtrunA17_Chr3g0109001 26.068 468 331 6 62 519 19 481 1.12e-49 183
MsaG030060 MtrunA17_Chr3g0109031 25.367 477 322 7 62 519 19 480 1.46e-49 182
MsaG030060 MtrunA17_Chr7g0233861 51.366 183 63 3 188 357 11 180 2.38e-49 172
MsaG030060 MtrunA17_Chr2g0319081 37.091 275 154 6 226 496 109 368 1.22e-48 178
MsaG030060 MtrunA17_Chr2g0319081 43.262 141 74 3 1 136 1 140 3.77e-26 112
MsaG030060 MtrunA17_Chr3g0108981 25.918 463 322 8 70 519 2 456 3.17e-48 178
MsaG030060 MtrunA17_Chr3g0108961 25.918 463 322 8 70 519 2 456 3.17e-48 178
MsaG030060 MtrunA17_Chr3g0108941 25.482 467 335 5 62 519 19 481 3.30e-48 179
MsaG030060 MtrunA17_Chr3g0109021 25.417 480 318 10 62 519 19 480 3.86e-47 176
MsaG030060 MtrunA17_Chr3g0105691 25.482 467 334 6 62 519 52 513 1.33e-46 175
MsaG030060 MtrunA17_Chr3g0109011 25.758 462 323 8 70 519 2 455 6.53e-46 172
MsaG030060 MtrunA17_Chr3g0108991 25.462 487 309 10 62 519 19 480 9.53e-46 172
MsaG030060 MtrunA17_Chr3g0108951 25.462 487 309 10 62 519 19 480 9.53e-46 172
MsaG030060 MtrunA17_Chr6g0465941 64.103 117 38 2 386 499 1 116 1.99e-45 160
MsaG030060 MtrunA17_Chr6g0466121 64.103 117 38 2 386 499 1 116 1.99e-45 160
MsaG030060 MtrunA17_Chr6g0465991 64.103 117 38 2 386 499 1 116 1.99e-45 160
MsaG030060 MtrunA17_Chr6g0466091 64.103 117 38 2 386 499 1 116 1.99e-45 160
MsaG030060 MtrunA17_Chr3g0099031 26.490 453 301 9 65 497 22 462 2.06e-45 171
MsaG030060 MtrunA17_Chr6g0465881 64.103 117 38 2 386 499 1 116 2.15e-45 160
MsaG030060 MtrunA17_Chr3g0108971 25.470 479 303 10 70 519 2 455 2.98e-45 170
MsaG030060 MtrunA17_Chr6g0477111 25.455 440 304 6 74 497 6 437 2.77e-44 167
MsaG030060 MtrunA17_Chr7g0237021 63.793 116 40 1 386 499 1 116 5.08e-44 156
MsaG030060 MtrunA17_Chr7g0236941 63.793 116 40 1 386 499 1 116 5.08e-44 156
MsaG030060 MtrunA17_Chr6g0477121 24.877 406 274 5 134 522 10 401 3.43e-40 153
MsaG030060 MtrunA17_Chr5g0445401 71.277 94 25 2 167 258 1 94 9.18e-37 133
MsaG030060 MtrunA17_Chr7g0217361 32.932 249 160 3 256 499 8 254 1.50e-36 140
MsaG030060 MtrunA17_Chr8g0357221 36.179 246 147 5 9 252 47 284 8.50e-36 137
MsaG030060 MtrunA17_Chr5g0445521 61.224 98 37 1 1 98 1 97 1.49e-35 130
MsaG030060 MtrunA17_Chr2g0310121 25.806 434 267 13 71 466 275 691 1.04e-33 139
MsaG030060 MtrunA17_Chr5g0445501 52.991 117 51 2 386 499 1 116 9.14e-33 124
MsaG030060 MtrunA17_Chr3g0101681 26.887 424 275 14 71 466 276 692 1.55e-31 132
MsaG030060 MtrunA17_Chr7g0236921 60.784 102 34 2 404 499 1 102 4.11e-31 119
MsaG030060 MtrunA17_Chr8g0357231 33.168 202 132 2 299 497 1 202 2.32e-30 120
MsaG030060 MtrunA17_Chr7g0236931 55.102 98 39 2 404 499 1 95 9.15e-27 106
MsaG030060 MtrunA17_Chr7g0218001 30.303 264 156 6 70 306 345 607 3.53e-23 105
MsaG030060 MtrunA17_Chr7g0259881 27.636 275 165 6 70 314 228 498 1.52e-22 103
MsaG030060 MtrunA17_Chr7g0217351 28.497 193 132 3 70 259 84 273 1.01e-18 87.8
MsaG030060 MtrunA17_Chr4g0034381 78.723 47 10 0 547 593 134 180 2.48e-17 82.8
MsaG030060 MtrunA17_Chr2g0281651 25.194 258 158 6 230 470 39 278 1.52e-16 82.8
MsaG030060 MtrunA17_Chr7g0218041 31.950 241 135 8 121 335 3 240 3.52e-16 82.0
MsaG030060 MtrunA17_Chr5g0396491 60.294 68 26 1 492 558 122 189 1.30e-15 80.5
MsaG030060 MtrunA17_Chr2g0328811 97.143 35 1 0 758 792 93 127 4.51e-15 78.2
MsaG030060 MtrunA17_Chr4g0038851 68.750 48 14 1 512 558 134 181 2.42e-13 73.2
MsaG030060 MtrunA17_Chr7g0258001 80.000 35 7 0 758 792 93 127 2.66e-12 69.3
MsaG030060 MtrunA17_Chr1g0173711 77.143 35 8 0 758 792 93 127 4.01e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030060 AT5G23960.2 38.900 491 289 6 13 497 3 488 7.22e-116 362
MsaG030060 AT5G23960.1 38.945 493 288 7 13 497 3 490 1.16e-114 359
MsaG030060 AT3G14490.1 36.905 504 306 7 17 513 60 558 2.71e-109 347
MsaG030060 AT3G14540.1 35.700 493 299 6 22 504 65 549 1.99e-100 324
MsaG030060 AT1G31950.1 34.286 490 310 6 22 504 70 554 5.91e-100 323
MsaG030060 AT1G70080.1 36.345 487 292 7 17 491 66 546 6.37e-100 323
MsaG030060 AT1G31950.3 34.420 491 309 7 22 504 70 555 1.16e-99 322
MsaG030060 AT3G14520.1 35.306 490 305 6 22 504 68 552 2.00e-98 318
MsaG030060 AT3G29410.1 35.167 509 307 8 17 513 64 561 4.21e-98 318
MsaG030060 AT1G33750.1 34.455 505 319 8 5 504 55 552 8.76e-98 317
MsaG030060 AT4G20230.1 35.305 524 316 10 27 541 72 581 6.52e-95 309
MsaG030060 AT4G20230.2 35.305 524 316 10 27 541 84 593 1.19e-94 309
MsaG030060 AT3G29410.2 35.857 449 280 5 70 513 11 456 6.05e-92 298
MsaG030060 AT3G32030.1 34.292 487 308 8 22 501 68 549 7.74e-92 301
MsaG030060 AT4G20200.1 35.246 488 293 8 36 504 70 553 8.92e-90 296
MsaG030060 AT3G14540.2 35.477 451 273 6 22 462 65 507 1.53e-89 292
MsaG030060 AT5G44630.1 35.464 485 295 9 18 490 14 492 2.55e-88 290
MsaG030060 AT1G31950.2 34.157 445 280 7 22 458 70 509 4.38e-88 289
MsaG030060 AT4G20210.1 32.772 534 332 10 17 533 58 581 2.97e-87 289
MsaG030060 AT4G16730.1 34.205 459 286 5 52 499 27 480 4.47e-82 273
MsaG030060 AT4G15870.1 33.535 495 299 12 5 490 59 532 3.26e-81 273
MsaG030060 AT4G13300.1 33.263 475 304 8 27 493 22 491 3.88e-81 271
MsaG030060 AT1G66020.1 34.631 488 309 7 27 507 66 550 4.82e-81 272
MsaG030060 AT3G29190.1 32.389 494 324 7 27 513 69 559 9.12e-81 271
MsaG030060 AT3G29110.1 33.333 495 320 8 17 504 55 546 2.26e-80 270
MsaG030060 AT4G16730.2 34.325 437 273 4 74 499 1 434 5.76e-80 266
MsaG030060 AT3G25810.1 33.333 498 309 9 14 496 44 533 2.43e-79 268
MsaG030060 AT2G24210.1 32.657 493 313 9 15 496 44 528 1.19e-78 265
MsaG030060 AT2G23230.2 33.608 485 307 9 16 490 55 534 1.32e-77 263
MsaG030060 AT4G16740.1 32.049 493 313 8 15 496 24 505 4.77e-77 260
MsaG030060 AT3G25820.1 32.129 498 313 9 14 496 44 531 5.01e-77 261
MsaG030060 AT3G25830.1 32.129 498 313 9 14 496 44 531 5.01e-77 261
MsaG030060 AT4G13280.2 32.983 476 308 7 25 493 20 491 1.57e-76 259
MsaG030060 AT1G48800.1 32.150 507 331 9 16 513 62 564 8.24e-76 258
MsaG030060 AT2G23230.1 33.264 484 305 9 16 490 55 529 4.04e-75 256
MsaG030060 AT4G13280.1 31.646 474 287 7 25 493 20 461 8.53e-69 237
MsaG030060 AT1G61680.1 29.451 455 304 7 54 499 77 523 1.01e-67 235
MsaG030060 AT1G48820.1 29.795 537 321 12 17 546 63 550 1.90e-66 231
MsaG030060 AT1G66020.2 37.607 351 211 6 27 372 66 413 5.99e-65 223
MsaG030060 AT1G61680.2 29.231 455 298 8 54 499 77 516 6.58e-65 227
MsaG030060 AT4G16740.2 34.037 379 229 7 15 382 24 392 6.20e-64 220
MsaG030060 AT3G25820.2 33.248 391 239 8 14 392 44 424 1.09e-63 221
MsaG030060 AT5G48110.1 35.691 311 197 3 217 525 2 311 4.40e-60 207
MsaG030060 AT4G16740.4 36.280 328 188 7 15 331 24 341 1.27e-55 196
MsaG030060 AT4G16740.3 36.280 328 188 7 15 331 24 341 1.27e-55 196
MsaG030060 AT3G25820.4 35.152 330 192 8 14 331 44 363 2.87e-54 193
MsaG030060 AT3G25820.3 35.152 330 192 8 14 331 44 363 2.87e-54 193
MsaG030060 AT1G17760.1 71.429 112 31 1 627 738 110 220 4.39e-48 182
MsaG030060 AT1G79460.1 24.832 447 301 13 71 489 274 713 6.01e-26 114
MsaG030060 AT4G02780.1 28.090 267 164 6 70 309 330 595 8.31e-21 98.6
MsaG030060 AT1G61120.1 23.222 478 296 18 72 508 289 736 7.35e-19 92.4
MsaG030060 AT4G34360.1 68.085 47 15 0 547 593 129 175 7.14e-15 75.9
MsaG030060 AT1G51260.1 82.857 35 6 0 758 792 93 127 1.48e-12 70.5
MsaG030060 AT1G50420.1 71.795 39 11 0 512 550 145 183 1.18e-11 68.6
MsaG030060 AT3G57650.1 74.286 35 9 0 758 792 92 126 2.69e-11 67.0

Find 152 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTACTCACTTTCGCAAAAT+TGG 0.149036 6:+1998068 MsaT030060.1:CDS
GCCCTCGCACTATTAAATTT+TGG 0.193492 6:-2011201 None:intergenic
GGTATATAGCCTTCCTTCTT+TGG 0.243745 6:-1998659 None:intergenic
GATACTTTCCTGAATAAATT+AGG 0.247966 6:+2009853 MsaT030060.1:CDS
GCACTCTTTCTGTGCTATTC+AGG 0.251378 6:+1997431 MsaT030060.1:CDS
TTCTACTATTTGTAGGCTAA+TGG 0.251691 6:+2000021 MsaT030060.1:CDS
TGCTAACACCTAATTTATTC+AGG 0.253669 6:-2009861 None:intergenic
AGTGACATTGATTGGCTTAT+TGG 0.258738 6:+2012723 MsaT030060.1:CDS
CAGATTTCCACCCTTGCGTT+TGG 0.263752 6:+1996797 MsaT030060.1:CDS
TTTAAATTCTTTCTTCATAA+TGG 0.266444 6:-2012944 None:intergenic
CACAAGAGCTATGTTGAAAA+TGG 0.276972 6:+1997382 MsaT030060.1:CDS
AGGATAAAATTGACAAAATT+AGG 0.280570 6:+1998363 MsaT030060.1:CDS
TATATCAGTCTTGAAGGTTT+TGG 0.282424 6:+1998615 MsaT030060.1:CDS
AGATTTCCACCCTTGCGTTT+GGG 0.302050 6:+1996798 MsaT030060.1:CDS
TCAAGCTATTGTTGAGTCTA+TGG 0.304822 6:+2009388 MsaT030060.1:CDS
GCATTTATCTCGATAACTTT+TGG 0.306930 6:+2009683 MsaT030060.1:CDS
AATTGATTCAATATGTCGTT+TGG 0.310092 6:+1997309 MsaT030060.1:CDS
GCATTGGCATTGGTATCTAA+TGG 0.316482 6:+1999977 MsaT030060.1:CDS
AGGAACAGAAATTTGCCTTT+TGG 0.316696 6:-2011296 None:intergenic
ATCATCAATGATCGATAATA+AGG 0.323892 6:-1998388 None:intergenic
AGGACATGTTTGCTCATTGC+TGG 0.326523 6:+2000198 MsaT030060.1:CDS
ATGCAATTTCTACTATTTGT+AGG 0.334193 6:+2000014 MsaT030060.1:CDS
GTCTATGGAAGGTGAGAAAA+TGG 0.336276 6:+2009403 MsaT030060.1:CDS
TTCCTTGTTCATCCTTGAAT+TGG 0.336424 6:-1997780 None:intergenic
CTACATTGAAAGGTGTTCAT+AGG 0.336704 6:+2009642 MsaT030060.1:CDS
ATTTCTTTGCAAGTCAGATT+TGG 0.353526 6:-1996770 None:intergenic
AAAGATTCTTCTTGCAAAAG+AGG 0.356688 6:+2008646 MsaT030060.1:CDS
CAGGATATATTACCAACTTC+TGG 0.358258 6:-2011075 None:intergenic
TAGGAACTTGCCTAGGTTAG+AGG 0.360571 6:+1997996 MsaT030060.1:CDS
CCCATCACATTGAACAACTT+TGG 0.363703 6:+2011020 MsaT030060.1:CDS
TTGGCTTATTGGATGGGTCT+TGG 0.364550 6:+2012734 MsaT030060.1:CDS
ATCTGATGTGTTTCTTCTTG+TGG 0.368845 6:-2010953 None:intergenic
TATAAGCTTGGATGACCATT+TGG 0.370342 6:-1999794 None:intergenic
AACGACATATTGAATCAATT+AGG 0.373042 6:-1997306 None:intergenic
CTGCAAGAAAAGGACTCAAT+TGG 0.379067 6:-1997939 None:intergenic
TATTGGATGGGTCTTGGCTC+AGG 0.379258 6:+2012740 MsaT030060.1:CDS
TGCTATTCAAGAATGCAAAA+AGG 0.392074 6:+2000258 MsaT030060.1:CDS
GATTGCTATATGAAGCAATA+TGG 0.394990 6:+2000220 MsaT030060.1:CDS
AATATATATATCAGTCTTGA+AGG 0.395546 6:+1998609 MsaT030060.1:CDS
ATCACAGGAGTGACATTGAT+TGG 0.396953 6:+2012715 MsaT030060.1:intron
ATTCCTTTCGATGAAGACTT+TGG 0.397735 6:-1998104 None:intergenic
AGCCTTTCTAAAGTCATTGC+CGG 0.398907 6:+2010830 MsaT030060.1:CDS
GGTTCATGGAGAGGAGATCT+TGG 0.401047 6:+1997870 MsaT030060.1:CDS
CATTGGCATTGGTATCTAAT+GGG 0.402353 6:+1999978 MsaT030060.1:CDS
AAAAGGAAATTGCAATTGCT+TGG 0.411682 6:+2000275 MsaT030060.1:CDS
TCCAAAGTTGTTCAATGTGA+TGG 0.413856 6:-2011021 None:intergenic
ATGCTTGCTGTACCACCAAC+TGG 0.416506 6:+2011270 MsaT030060.1:CDS
AGTCTTCATCGAAAGGAATT+CGG 0.418759 6:+1998108 MsaT030060.1:CDS
ATTGATTCAATATGTCGTTT+GGG 0.419389 6:+1997310 MsaT030060.1:CDS
ATACTGAGGCTCAAAATATA+CGG 0.420977 6:-1998331 None:intergenic
CTAACCTAGGCAAGTTCCTA+TGG 0.422143 6:-1997993 None:intergenic
CTTATATAACTGAAGCTAGA+TGG 0.426963 6:+1999811 MsaT030060.1:CDS
GCCTCACATATGATGGTTCA+TGG 0.441351 6:+1997856 MsaT030060.1:CDS
TATATTAATTTCATGCAGTC+AGG 0.445660 6:-1998592 None:intergenic
GGATTTGAAATCAAAGCTAT+AGG 0.445820 6:-2009803 None:intergenic
AATCAACCTTTCAATTGCCT+TGG 0.449411 6:-1998454 None:intergenic
TAGCTTCAGTTATATAAGCT+TGG 0.457467 6:-1999806 None:intergenic
CTGTGCTATTCAGGCTGTTG+AGG 0.467904 6:+1997440 MsaT030060.1:CDS
GTTTCAGACTTCGGATTCCA+TGG 0.472468 6:-2009602 None:intergenic
ACAATCCTATCTCGTACATA+TGG 0.476526 6:-1998285 None:intergenic
ATGACTGAAGCAAGCTGCAT+TGG 0.480305 6:-1996743 None:intergenic
ACCTACCATATGTACGAGAT+AGG 0.481504 6:+1998280 MsaT030060.1:CDS
TCACAGCGCACGGGTTGCCT+TGG 0.481689 6:+2012910 MsaT030060.1:intron
TACATTGAAAGGTGTTCATA+GGG 0.482070 6:+2009643 MsaT030060.1:CDS
TGAAGCAATATGGTATGTCT+AGG 0.485271 6:+2000230 MsaT030060.1:CDS
CATTGATTGGCTTATTGGAT+GGG 0.486891 6:+2012728 MsaT030060.1:CDS
AGATGTGTTCAACCAATTCA+AGG 0.491227 6:+1997768 MsaT030060.1:intron
TTGACTTGTGCTGCAAGAAA+AGG 0.492272 6:-1997949 None:intergenic
CAGATTCTGAATCATATTGA+AGG 0.493714 6:-1996831 None:intergenic
AGCATCAGGCCCTGTGTGGA+TGG 0.495690 6:+2010800 MsaT030060.1:CDS
ACATAGTTTGAACATAAAAG+AGG 0.496267 6:+2000178 MsaT030060.1:intron
GAAGAAGATATGACCAAAGA+AGG 0.498444 6:+1998646 MsaT030060.1:CDS
TTATTCCAGGTTTCATCCAC+GGG 0.502504 6:+2013321 MsaT030060.1:intron
GATTTCCACCCTTGCGTTTG+GGG 0.503881 6:+1996799 MsaT030060.1:CDS
CCAAAGTTGTTCAATGTGAT+GGG 0.504943 6:-2011020 None:intergenic
TGCAAGAAAAGGACTCAATT+GGG 0.509534 6:-1997938 None:intergenic
GTGTTTATGGCAGTTCCAAA+TGG 0.510808 6:+1999779 MsaT030060.1:intron
TTCCTTGAGTCCCTCTTGTG+AGG 0.511801 6:-2013370 None:intergenic
AACAAGTTCTTACATTGGCA+TGG 0.512477 6:+1999911 MsaT030060.1:CDS
GGATCAGACATAATAGCATC+AGG 0.520650 6:+2010786 MsaT030060.1:intron
TGGAGAGGAGATCTTGGAAA+AGG 0.522985 6:+1997876 MsaT030060.1:CDS
TGCATGTATTCTTCTACCGT+TGG 0.524315 6:-1999852 None:intergenic
CACGGGCTCTTCACAAGAAG+TGG 0.524867 6:+2013338 MsaT030060.1:CDS
ACCACCAACTGGATCCCAAA+AGG 0.534706 6:+2011281 MsaT030060.1:CDS
AAAAGAGCAATCCATTGTGC+AGG 0.535750 6:-2009455 None:intergenic
GAAAATGTTGATGATACAGC+AGG 0.537015 6:+2009886 MsaT030060.1:CDS
TTGGACATAGTTTCAGACTT+CGG 0.539407 6:-2009611 None:intergenic
ACATTGATTGGCTTATTGGA+TGG 0.541072 6:+2012727 MsaT030060.1:CDS
AACCGGCAATGACTTTAGAA+AGG 0.541989 6:-2010832 None:intergenic
AAGTTCTGTTAGCCAGAAGT+TGG 0.544913 6:+2011063 MsaT030060.1:CDS
CTTATTCCAGGTTTCATCCA+CGG 0.547800 6:+2013320 MsaT030060.1:intron
CTCACAACAAGTTCTTACAT+TGG 0.548128 6:+1999906 MsaT030060.1:CDS
AGACTTATCAGAGATGTTGA+GGG 0.548429 6:+1997814 MsaT030060.1:CDS
GGAGATCTTGGAAAAGGCAT+TGG 0.550396 6:+1997882 MsaT030060.1:CDS
CACTTCTTGTGAAGAGCCCG+TGG 0.552358 6:-2013337 None:intergenic
AACCAAAATTTAATAGTGCG+AGG 0.552564 6:+2011199 MsaT030060.1:CDS
TTTCGTTGGTCACAGCGCAC+GGG 0.554146 6:+2012901 MsaT030060.1:intron
AAGACTTATCAGAGATGTTG+AGG 0.554482 6:+1997813 MsaT030060.1:CDS
AGCCTCAGTATTCTCAAGCA+AGG 0.554659 6:+1998343 MsaT030060.1:CDS
TGAACTTTATAGATGGTGGA+AGG 0.555716 6:+1998239 MsaT030060.1:intron
GATGATACATATGATGCATA+CGG 0.559369 6:+1998405 MsaT030060.1:CDS
GTATGAAGCCTCACATATGA+TGG 0.561116 6:+1997849 MsaT030060.1:CDS
AACATTATTAACATTTGCAG+AGG 0.563575 6:-2011316 None:intergenic
CGAGGGCAGTATACAGAGAA+CGG 0.564609 6:+2011217 MsaT030060.1:CDS
AAGGTCATGTCTACATTGAA+AGG 0.565200 6:+2009632 MsaT030060.1:CDS
TACCTTCGTGCCTCTAACCT+AGG 0.565456 6:-1998006 None:intergenic
TTCAGGCTGTTGAGGCAACA+AGG 0.566827 6:+1997448 MsaT030060.1:CDS
AGCCTCACAAGAGGGACTCA+AGG 0.568328 6:+2013368 MsaT030060.1:CDS
AGGACTCAATTGGGTAACAA+TGG 0.569957 6:-1997929 None:intergenic
TTTCAGACTTCGGATTCCAT+GGG 0.572108 6:-2009601 None:intergenic
ATTTCCACCCTTGCGTTTGG+GGG 0.574731 6:+1996800 MsaT030060.1:CDS
TCTCCAAAGTCTTCATCGAA+AGG 0.575901 6:+1998101 MsaT030060.1:CDS
ACCAAAATTTAATAGTGCGA+GGG 0.577057 6:+2011200 MsaT030060.1:CDS
GAAGCAATATGGTATGTCTA+GGG 0.580976 6:+2000231 MsaT030060.1:CDS
TTTCAATGTAGACATGACCT+TGG 0.582305 6:-2009630 None:intergenic
AGTCAAACATAGCTTAAAGC+AGG 0.582484 6:+1997966 MsaT030060.1:CDS
TATAGGATAGTGGTGACCCA+TGG 0.588816 6:+2009585 MsaT030060.1:intron
GCTTAAAGCAGGCTCTCCAT+AGG 0.589217 6:+1997977 MsaT030060.1:CDS
TTTCCACCCTTGCGTTTGGG+GGG 0.591414 6:+1996801 MsaT030060.1:CDS
TCAATATGATTCAGAATCTG+TGG 0.591957 6:+1996834 MsaT030060.1:CDS
AAGATATGACCAAAGAAGGA+AGG 0.593350 6:+1998650 MsaT030060.1:CDS
AGTTGTTCAATGTGATGGGT+AGG 0.596093 6:-2011016 None:intergenic
ACAAGTTCTTACATTGGCAT+GGG 0.598085 6:+1999912 MsaT030060.1:CDS
AGTAGAGTTTCATTGTGTGA+AGG 0.598718 6:-1998051 None:intergenic
ATTGGGTAACAATGGACTCA+AGG 0.606105 6:-1997921 None:intergenic
TTGTGAGGAAAGTCTATCAG+AGG 0.607948 6:+2010981 MsaT030060.1:CDS
GATGCATACTAGGTGATACA+TGG 0.610298 6:-1997474 None:intergenic
GAAGTCTGAAACTATGTCCA+AGG 0.615623 6:+2009613 MsaT030060.1:CDS
GCTATTGTTGAGTCTATGGA+AGG 0.617292 6:+2009392 MsaT030060.1:CDS
ACATATGATGGTTCATGGAG+AGG 0.617690 6:+1997861 MsaT030060.1:CDS
TAATGGCCATCGTGCTACCA+AGG 0.618127 6:-2012927 None:intergenic
CTCTCCATAGGAACTTGCCT+AGG 0.625089 6:+1997989 MsaT030060.1:CDS
GCTATGTACTCCATCCACAC+AGG 0.627247 6:-2010810 None:intergenic
ATCCTTGCTTGAGAATACTG+AGG 0.627415 6:-1998345 None:intergenic
CACATCAGATGACTGTTGTG+AGG 0.630283 6:+2010966 MsaT030060.1:CDS
CAACAATAACTATGTACCAA+CGG 0.630829 6:+1999836 MsaT030060.1:CDS
AACCAATTCAAGGATGAACA+AGG 0.633577 6:+1997778 MsaT030060.1:CDS
CTATGTACTCCATCCACACA+GGG 0.637483 6:-2010809 None:intergenic
GAGATCAAAGCCTCACAAGA+GGG 0.639033 6:+2013360 MsaT030060.1:CDS
GGAGATCAAAGCCTCACAAG+AGG 0.641381 6:+2013359 MsaT030060.1:CDS
CAGGTGGGATATCAGCAGCA+TGG 0.642839 6:+1998561 MsaT030060.1:intron
TAATAGCATCAGGCCCTGTG+TGG 0.645875 6:+2010796 MsaT030060.1:CDS
GACTTATCAGAGATGTTGAG+GGG 0.646184 6:+1997815 MsaT030060.1:CDS
TCCATGAACCATCATATGTG+AGG 0.647503 6:-1997857 None:intergenic
ATGCTGCTGAACCTGCACAA+TGG 0.650823 6:+2009444 MsaT030060.1:CDS
TCCTATCTCGTACATATGGT+AGG 0.653713 6:-1998281 None:intergenic
ATATGTACGAGATAGGATTG+TGG 0.678344 6:+1998287 MsaT030060.1:CDS
TGTGAGGAAAGTCTATCAGA+GGG 0.681127 6:+2010982 MsaT030060.1:CDS
ATAGCACAGAAAGAGTGCAA+AGG 0.691827 6:-1997426 None:intergenic
GGGTTGCCTTGGTAGCACGA+TGG 0.694385 6:+2012921 MsaT030060.1:CDS
TGCCTAGGTTAGAGGCACGA+AGG 0.706271 6:+1998004 MsaT030060.1:CDS
GGATGAAATTGTGACCAGTG+AGG 0.787653 6:+2000042 MsaT030060.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 1996739 2013404 1996739 ID=MsaG030060
Chr6 mRNA 1996739 2013404 1996739 ID=MsaT030060.1;Parent=MsaG030060
Chr6 exon 1996739 1996855 1996739 ID=MsaT030060.1.exon1;Parent=MsaT030060.1
Chr6 CDS 1996739 1996855 1996739 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 1997205 1997487 1997205 ID=MsaT030060.1.exon2;Parent=MsaT030060.1
Chr6 CDS 1997205 1997487 1997205 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 1997770 1998142 1997770 ID=MsaT030060.1.exon3;Parent=MsaT030060.1
Chr6 CDS 1997770 1998142 1997770 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 1998251 1998469 1998251 ID=MsaT030060.1.exon4;Parent=MsaT030060.1
Chr6 CDS 1998251 1998469 1998251 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 1998564 1998702 1998564 ID=MsaT030060.1.exon5;Parent=MsaT030060.1
Chr6 CDS 1998564 1998702 1998564 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 1999792 1999933 1999792 ID=MsaT030060.1.exon6;Parent=MsaT030060.1
Chr6 CDS 1999792 1999933 1999792 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 1999963 2000063 1999963 ID=MsaT030060.1.exon7;Parent=MsaT030060.1
Chr6 CDS 1999963 2000063 1999963 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 2000184 2000303 2000184 ID=MsaT030060.1.exon8;Parent=MsaT030060.1
Chr6 CDS 2000184 2000303 2000184 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 2008626 2008667 2008626 ID=MsaT030060.1.exon9;Parent=MsaT030060.1
Chr6 CDS 2008626 2008667 2008626 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 2009368 2009481 2009368 ID=MsaT030060.1.exon10;Parent=MsaT030060.1
Chr6 CDS 2009368 2009481 2009368 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 2009590 2009709 2009590 ID=MsaT030060.1.exon11;Parent=MsaT030060.1
Chr6 CDS 2009590 2009709 2009590 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 2009799 2009907 2009799 ID=MsaT030060.1.exon12;Parent=MsaT030060.1
Chr6 CDS 2009799 2009907 2009799 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 2010787 2010851 2010787 ID=MsaT030060.1.exon13;Parent=MsaT030060.1
Chr6 CDS 2010787 2010851 2010787 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 2010944 2011084 2010944 ID=MsaT030060.1.exon14;Parent=MsaT030060.1
Chr6 CDS 2010944 2011084 2010944 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 2011174 2011326 2011174 ID=MsaT030060.1.exon15;Parent=MsaT030060.1
Chr6 CDS 2011174 2011326 2011174 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 2011807 2011841 2011807 ID=MsaT030060.1.exon16;Parent=MsaT030060.1
Chr6 CDS 2011807 2011841 2011807 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 2012722 2012761 2012722 ID=MsaT030060.1.exon17;Parent=MsaT030060.1
Chr6 CDS 2012722 2012761 2012722 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 2012916 2012975 2012916 ID=MsaT030060.1.exon18;Parent=MsaT030060.1
Chr6 CDS 2012916 2012975 2012916 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Chr6 exon 2013330 2013404 2013330 ID=MsaT030060.1.exon19;Parent=MsaT030060.1
Chr6 CDS 2013330 2013404 2013330 ID=cds.MsaT030060.1;Parent=MsaT030060.1
Gene Sequence

>MsaT030060.1

ATGTCCAATGCAGCTTGCTTCAGTCATGATGCCAAATCTGACTTGCAAAGAAATCTTGCAGATTTCCACCCTTGCGTTTGGGGGGATTATTTCCTTCAATATGATTCAGAATCTGTGGAGCTTGATCACAATATCTCAGCACAAATTGATGCACTAAAAAAGGAAGTGAGAAACATGTTTAATATCTCAAAAACTGAAAAGCCCTCGGAAAAAGTTAACCTAATTGATTCAATATGTCGTTTGGGTGTGAGCTATCATTACGAAAATGAGATTGATGAATTTATGCAATACATTCACAAGAGCTATGTTGAAAATGGAGAAATAACTCTTGAAGACAACCTTTGCACTCTTTCTGTGCTATTCAGGCTGTTGAGGCAACAAGGATTCCATGTATCACCTAATGTGTTCAACCAATTCAAGGATGAACAAGGAAACTTTAGTGAAAGACTTATCAGAGATGTTGAGGGGATGTTAAGCTTGTATGAAGCCTCACATATGATGGTTCATGGAGAGGAGATCTTGGAAAAGGCATTGGCATTTACTTCAACTCACCTTGAGTCCATTGTTACCCAATTGAGTCCTTTTCTTGCAGCACAAGTCAAACATAGCTTAAAGCAGGCTCTCCATAGGAACTTGCCTAGGTTAGAGGCACGAAGGTACATTTCAATCTATGAGAAAGATCCTTCACACAATGAAACTCTACTCACTTTCGCAAAATTGGATTTCAATATTCTCCAAAGTCTTCATCGAAAGGAATTCGGAAATATTTGCAAATGGTGGAAGGAGTTCGATATCCCTAAAAACCTACCATATGTACGAGATAGGATTGTGGAAATGTGTTTTTGGGTCTTGGCCGTATATTTTGAGCCTCAGTATTCTCAAGCAAGGATAAAATTGACAAAATTAGGTGCCTTATTATCGATCATTGATGATACATATGATGCATACGGAAGCATTAATGAACTAGAACTTTTTACCAAGGCAATTGAAAGGTGGGATATCAGCAGCATGGACGATCTTCCTGACTGCATGAAATTAATATATATATCAGTCTTGAAGGTTTTGGAAGAAGTAGAAGAAGATATGACCAAAGAAGGAAGGCTATATACCCTTAAATACTATAAAAAAGAATTCCAAATGGTCATCCAAGCTTATATAACTGAAGCTAGATGGTTCAACAATAACTATGTACCAACGGTAGAAGAATACATGCACATATCAAAAAGAACATGTTGTCAATCATTACTCACAACAAGTTCTTACATTGGCATGGTGGGCCAAAAATTGGCATTGGCATTGGTATCTAATGGGCCAAAAATTGTGGATGCAATTTCTACTATTTGTAGGCTAATGGATGAAATTGTGACCAGTGAGTTTGAACATAAAAGAGGACATGTTTGCTCATTGCTGGATTGCTATATGAAGCAATATGGTATGTCTAGGGAAGCTGCTATTCAAGAATGCAAAAAGGAAATTGCAATTGCTTGGAAATATATCTTGGACAATTATATCATAAAGATTCTTCTTGCAAAAGAGGTAGCATATATTTTTACAAATCAAGCTATTGTTGAGTCTATGGAAGGTGAGAAAATGGTTCATATAATTGATCTTAATGCTGCTGAACCTGCACAATGGATTGCTCTTTTACAAGATAGTGGTGACCCATGGAATCCGAAGTCTGAAACTATGTCCAAGGTCATGTCTACATTGAAAGGTGTTCATAGGGTTTTGAAAGCAAATGGCGCATTTATCTCGATAACTTTTGGCCAGGCGTCCTATAGCTTTGATTTCAAATCCTTTTTTCATGCAGTACTTGATTTAAGCGATACTTTCCTGAATAAATTAGGTGTTAGCAGAGAAAATGTTGATGATACAGCAGGATCAGACATAATAGCATCAGGCCCTGTGTGGATGGAGTACATAGCCTTTCTAAAGTCATTGCCGGCTGCACATCCACAAGAAGAAACACATCAGATGACTGTTGTGAGGAAAGTCTATCAGAGGGCTATTATTACTCCTACCCATCACATTGAACAACTTTGGAAAGACTATGATAGTTTTGAAAGTTCTGTTAGCCAGAAGTTGGCTAGAGGGCTTATATCCGAATATCAACCAAAATTTAATAGTGCGAGGGCAGTATACAGAGAACGGAAGAAGTTTTTTGATGAAATTGATTGGAATATGCTTGCTGTACCACCAACTGGATCCCAAAAGGCAAATTTCTGTTCCTCTGCAAATATAGAAACTGAGTTTGTTGAAGTTTTAGCATTCAGGAGTGACATTGATTGGCTTATTGGATGGGTCTTGGCTCAGCGCACGGGTTGCCTTGGTAGCACGATGGCCATTATGAAGAAAGAATTTAAATACCTCCCTGTTTCATCCACGGGCTCTTCACAAGAAGTGGAGATCAAAGCCTCACAAGAGGGACTCAAGGAATTTCAAACCTGA
Protein sequence

>MsaT030060.1

MSNAACFSHDAKSDLQRNLADFHPCVWGDYFLQYDSESVELDHNISAQIDALKKEVRNMFNISKTEKPSEKVNLIDSICRLGVSYHYENEIDEFMQYIHKSYVENGEITLEDNLCTLSVLFRLLRQQGFHVSPNVFNQFKDEQGNFSERLIRDVEGMLSLYEASHMMVHGEEILEKALAFTSTHLESIVTQLSPFLAAQVKHSLKQALHRNLPRLEARRYISIYEKDPSHNETLLTFAKLDFNILQSLHRKEFGNICKWWKEFDIPKNLPYVRDRIVEMCFWVLAVYFEPQYSQARIKLTKLGALLSIIDDTYDAYGSINELELFTKAIERWDISSMDDLPDCMKLIYISVLKVLEEVEEDMTKEGRLYTLKYYKKEFQMVIQAYITEARWFNNNYVPTVEEYMHISKRTCCQSLLTTSSYIGMVGQKLALALVSNGPKIVDAISTICRLMDEIVTSEFEHKRGHVCSLLDCYMKQYGMSREAAIQECKKEIAIAWKYILDNYIIKILLAKEVAYIFTNQAIVESMEGEKMVHIIDLNAAEPAQWIALLQDSGDPWNPKSETMSKVMSTLKGVHRVLKANGAFISITFGQASYSFDFKSFFHAVLDLSDTFLNKLGVSRENVDDTAGSDIIASGPVWMEYIAFLKSLPAAHPQEETHQMTVVRKVYQRAIITPTHHIEQLWKDYDSFESSVSQKLARGLISEYQPKFNSARAVYRERKKFFDEIDWNMLAVPPTGSQKANFCSSANIETEFVEVLAFRSDIDWLIGWVLAQRTGCLGSTMAIMKKEFKYLPVSSTGSSQEVEIKASQEGLKEFQT*