AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG030090


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030090 MtrunA17_Chr6g0451441 93.654 457 18 1 1 446 1 457 0.0 884
MsaG030090 MtrunA17_Chr5g0441581 51.550 258 71 3 1 256 1 206 2.53e-74 246
MsaG030090 MtrunA17_Chr6g0451421 55.714 140 54 1 307 446 6 137 4.63e-41 143
MsaG030090 MtrunA17_Chr6g0451411 51.049 143 62 1 304 446 171 305 8.06e-36 134
MsaG030090 MtrunA17_Chr2g0325301 44.138 145 73 1 302 446 3 139 3.82e-33 122
MsaG030090 MtrunA17_Chr2g0329701 47.101 138 65 1 309 446 234 363 1.13e-30 122
MsaG030090 MtrunA17_Chr7g0264421 46.377 138 66 1 309 446 204 333 2.87e-29 117
MsaG030090 MtrunA17_Chr2g0296781 32.919 161 101 4 80 236 1 158 2.36e-17 84.7
MsaG030090 MtrunA17_Chr6g0451451 48.684 76 39 0 371 446 1 76 1.80e-15 71.6
MsaG030090 MtrunA17_Chr7g0258861 26.857 175 110 3 74 236 8 176 3.30e-15 76.3
MsaG030090 MtrunA17_Chr2g0324011 27.219 169 116 3 74 236 8 175 8.43e-14 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030090 AT3G01410.2 47.222 144 68 1 303 446 148 283 3.28e-31 122
MsaG030090 AT3G01410.1 47.222 144 68 1 303 446 148 283 3.28e-31 122
MsaG030090 AT5G51080.3 46.043 139 67 1 309 447 122 252 1.75e-27 110
MsaG030090 AT5G51080.1 46.043 139 67 1 309 447 185 315 6.57e-27 110
MsaG030090 AT5G51080.2 46.043 139 67 1 309 447 185 315 6.57e-27 110
MsaG030090 AT1G24090.1 44.667 150 73 2 299 446 204 345 1.14e-25 107

Find 89 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGGATAGTTCAAAGAATT+TGG 0.188161 6:-2365258 MsaT030090.1:CDS
TGGAGAGTATGCTTTGAAAA+AGG 0.195927 6:-2364982 MsaT030090.1:CDS
CCCGCATCTCGTTTGCAAAA+TGG 0.205635 6:+2364594 None:intergenic
TATGTTCCTTTGTAGCTCTA+TGG 0.232618 6:-2362830 MsaT030090.1:intron
TTGAACAATCTTCCCTTTGC+AGG 0.266353 6:+2364738 None:intergenic
GAGTATCGGGCTTTACTCTT+AGG 0.286694 6:-2362767 MsaT030090.1:CDS
GAAATTATGTGCTGAGGCTT+TGG 0.322454 6:-2362278 MsaT030090.1:CDS
CTCCTGGATCATCAGTGCTT+TGG 0.328920 6:+2364676 None:intergenic
TCCGCATCCAACACATTTCC+AGG 0.335217 6:-2362231 MsaT030090.1:CDS
AACATTTCTCACCTGTTGTT+TGG 0.340587 6:+2364139 None:intergenic
CTCGGAACTTGTTATCAATC+AGG 0.350472 6:-2362693 MsaT030090.1:intron
GCACGTTGGAAGCCTGAAAC+AGG 0.364470 6:-2365044 MsaT030090.1:CDS
CAACCTCTTGTTAAAGATGT+AGG 0.366945 6:-2364633 MsaT030090.1:CDS
ATGTTTAAGGAACGTAACTA+TGG 0.371416 6:-2361777 MsaT030090.1:intron
CTTTCTTGTATCCTTGAGTT+TGG 0.375582 6:-2363020 MsaT030090.1:CDS
ATGTTCCTTTGTAGCTCTAT+GGG 0.375904 6:-2362829 MsaT030090.1:intron
ACATCAAGAAAAGGAGATAA+TGG 0.382507 6:-2364939 MsaT030090.1:CDS
TTCCAACTTAATGCAAATCT+TGG 0.384721 6:+2364818 None:intergenic
GAAGATTGTTCAAGGCTAAC+AGG 0.389692 6:-2364728 MsaT030090.1:CDS
CTATGGGTTTCGCCAAGGAC+TGG 0.394553 6:-2362813 MsaT030090.1:CDS
GGTCTAACCGTTGAGATTGA+TGG 0.403981 6:+2361731 None:intergenic
TGAGTTTGGCGGTGTATCCA+TGG 0.404103 6:-2363006 MsaT030090.1:CDS
GCTTTACTGCTTGCTGAAGA+TGG 0.412974 6:-2362933 MsaT030090.1:CDS
TACAAGGAACGACATGCAAT+TGG 0.415503 6:-2364561 MsaT030090.1:CDS
AACAAGTTCCGAGTCTCCTT+TGG 0.419868 6:+2362703 None:intergenic
GAGTTTGGCGGTGTATCCAT+GGG 0.427150 6:-2363005 MsaT030090.1:CDS
AAAAGAGTCCGCTGAGTATC+GGG 0.427646 6:-2362780 MsaT030090.1:CDS
GATGCCTTTGGTACACTTCA+AGG 0.433112 6:-2364197 MsaT030090.1:CDS
TTTCTCACCTGTTGTTTGGT+TGG 0.444170 6:+2364143 None:intergenic
AACTTAATGCAAATCTTGGC+TGG 0.446598 6:+2364822 None:intergenic
TTGTTTGGTGACCCAGTCCT+TGG 0.447831 6:+2362801 None:intergenic
AGCCAAGATTTGCATTAAGT+TGG 0.449507 6:-2364820 MsaT030090.1:CDS
TGCAACAGATTCTGGATCCC+TGG 0.450769 6:-2362324 MsaT030090.1:intron
GTCTTCTTGATCCTCACTCC+TGG 0.453631 6:+2364660 None:intergenic
AACCGTTGAGATTGATGGCC+CGG 0.454084 6:+2361736 None:intergenic
GAGCTGTTCGTAATGCAAAC+CGG 0.456358 6:-2361755 MsaT030090.1:CDS
CAAAAGAGTCCGCTGAGTAT+CGG 0.473133 6:-2362781 MsaT030090.1:CDS
AACATTAATCGTGGACCAGC+AGG 0.474775 6:-2362963 MsaT030090.1:CDS
CTCAGAGCTGCTAGAAAAGA+TGG 0.475776 6:-2364252 MsaT030090.1:CDS
TATGGGTTTCGCCAAGGACT+GGG 0.476797 6:-2362812 MsaT030090.1:CDS
CTTTACTGCTTGCTGAAGAT+GGG 0.482053 6:-2362932 MsaT030090.1:CDS
GCTTGCTGAAGATGGGAGTC+TGG 0.485253 6:-2362925 MsaT030090.1:intron
TCTTGTATCCTTGAGTTTGG+CGG 0.487937 6:-2363017 MsaT030090.1:CDS
AATCGAATCACAGATGCCTT+TGG 0.490469 6:-2364209 MsaT030090.1:CDS
CAGGGCTAGTCTCGTGAGAA+TGG 0.497072 6:+2364757 None:intergenic
AGTTGGAAGACGATGGTTTG+TGG 0.499523 6:-2364803 MsaT030090.1:CDS
TCAAAGAATTTGGTAGATTG+TGG 0.500305 6:-2365248 MsaT030090.1:CDS
AAGATTAGAACATCAAGAAA+AGG 0.501587 6:-2364948 MsaT030090.1:CDS
CGACATGCAATTGGAAAAGA+AGG 0.505598 6:-2364552 MsaT030090.1:intron
TGCAAAGGGAAGATTGTTCA+AGG 0.507962 6:-2364736 MsaT030090.1:CDS
AGTTAAACCAACCAAACAAC+AGG 0.510461 6:-2364150 MsaT030090.1:intron
TTTAAGTATGTTACTGCCAA+AGG 0.514586 6:-2362719 MsaT030090.1:CDS
TTGAAACATGCGAGTATGAA+AGG 0.526301 6:-2362743 MsaT030090.1:CDS
AATCGTGGACCAGCAGGTGC+AGG 0.529975 6:-2362957 MsaT030090.1:CDS
GATAACTCTAATGATAATGA+AGG 0.530992 6:-2365131 MsaT030090.1:CDS
TAAATACCCTGGAAATGTGT+TGG 0.533351 6:+2362224 None:intergenic
TGCGGGAAATTATGTTTACA+AGG 0.537391 6:-2364577 MsaT030090.1:CDS
TGAACAATCTTCCCTTTGCA+GGG 0.541033 6:+2364739 None:intergenic
ATCCAAAGCACTGATGATCC+AGG 0.543376 6:-2364678 MsaT030090.1:CDS
TTTAAAGAAATTATGTGCTG+AGG 0.544923 6:-2362284 MsaT030090.1:CDS
TAGCTCTATGGGTTTCGCCA+AGG 0.553648 6:-2362818 MsaT030090.1:CDS
TGTGGTACTATTCATGATGA+AGG 0.556353 6:-2365230 MsaT030090.1:CDS
CACGAGACTAGCCCTGCAAA+GGG 0.557494 6:-2364750 MsaT030090.1:CDS
TCACGAGACTAGCCCTGCAA+AGG 0.558154 6:-2364751 MsaT030090.1:CDS
AGTAAAGCTCCTGCACCTGC+TGG 0.558433 6:+2362948 None:intergenic
GATAACTCGAATGATAATGA+AGG 0.559715 6:-2365164 MsaT030090.1:CDS
GATAACTCGAATGATAATGA+AGG 0.559715 6:-2365197 MsaT030090.1:CDS
TGGATACACCGCCAAACTCA+AGG 0.570316 6:+2363009 None:intergenic
AGACAACAACACAAGAATGA+TGG 0.575149 6:-2364324 MsaT030090.1:CDS
AATTCTTGCTTGCTCAAGAG+AGG 0.580330 6:-2364901 MsaT030090.1:CDS
ATGCCTTTGGTACACTTCAA+GGG 0.587883 6:-2364196 MsaT030090.1:CDS
TGCATTAAGTTGGAAGACGA+TGG 0.588214 6:-2364810 MsaT030090.1:CDS
AGCTGTTCGTAATGCAAACC+GGG 0.588529 6:-2361754 MsaT030090.1:CDS
AGCTGCTAGAAAAGATGGAG+TGG 0.602196 6:-2364247 MsaT030090.1:CDS
ATTCTTGCTTGCTCAAGAGA+GGG 0.604718 6:-2364900 MsaT030090.1:CDS
CCCTGGAAATGTGTTGGATG+CGG 0.605910 6:+2362230 None:intergenic
GCTCCTACATCTTTAACAAG+AGG 0.607465 6:+2364630 None:intergenic
TGTTACTGCCAAAGGAGACT+CGG 0.617844 6:-2362711 MsaT030090.1:CDS
CCGCATCCAACACATTTCCA+GGG 0.618262 6:-2362230 MsaT030090.1:intron
AGTTTGGCGGTGTATCCATG+GGG 0.626952 6:-2363004 MsaT030090.1:CDS
CACGTTGGAAGCCTGAAACA+GGG 0.628231 6:-2365043 MsaT030090.1:CDS
AACCGGGCCATCAATCTCAA+CGG 0.633568 6:-2361738 MsaT030090.1:CDS
GGTGATCTAAACATTAATCG+TGG 0.639032 6:-2362972 MsaT030090.1:CDS
ACACTTCAAGGGTATGCGCA+TGG 0.648714 6:-2364185 MsaT030090.1:CDS
GCACTGATGATCCAGGAGTG+AGG 0.654669 6:-2364671 MsaT030090.1:CDS
ATACCCTTGAAGTGTACCAA+AGG 0.664715 6:+2364193 None:intergenic
CTAATGATGATGAAGCACGT+TGG 0.674855 6:-2365058 MsaT030090.1:CDS
GTTTGGCGGTGTATCCATGG+GGG 0.709941 6:-2363003 MsaT030090.1:CDS
GAGTAAAGCCCGATACTCAG+CGG 0.716233 6:+2362772 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 2361734 2365279 2361734 ID=MsaG030090
Chr6 mRNA 2361734 2365279 2361734 ID=MsaT030090.1;Parent=MsaG030090
Chr6 exon 2361734 2361790 2361734 ID=MsaT030090.1.exon6;Parent=MsaT030090.1
Chr6 CDS 2361734 2361790 2361734 ID=cds.MsaT030090.1;Parent=MsaT030090.1
Chr6 exon 2362231 2362338 2362231 ID=MsaT030090.1.exon5;Parent=MsaT030090.1
Chr6 CDS 2362231 2362338 2362231 ID=cds.MsaT030090.1;Parent=MsaT030090.1
Chr6 exon 2362694 2362837 2362694 ID=MsaT030090.1.exon4;Parent=MsaT030090.1
Chr6 CDS 2362694 2362837 2362694 ID=cds.MsaT030090.1;Parent=MsaT030090.1
Chr6 exon 2362926 2363042 2362926 ID=MsaT030090.1.exon3;Parent=MsaT030090.1
Chr6 CDS 2362926 2363042 2362926 ID=cds.MsaT030090.1;Parent=MsaT030090.1
Chr6 exon 2364151 2364344 2364151 ID=MsaT030090.1.exon2;Parent=MsaT030090.1
Chr6 CDS 2364151 2364344 2364151 ID=cds.MsaT030090.1;Parent=MsaT030090.1
Chr6 exon 2364553 2365279 2364553 ID=MsaT030090.1.exon1;Parent=MsaT030090.1
Chr6 CDS 2364553 2365279 2364553 ID=cds.MsaT030090.1;Parent=MsaT030090.1
Gene Sequence

>MsaT030090.1

ATGGATAGTTCAAAGAATTTGGTAGATTGTGGTACTATTCATGATGAAGGTATAGAAGTTGATAACTCGAATGATAATGAAGGTATAGAAGTTGATAACTCGAATGATAATGAAGGTATAGAAGTTGATAACTCTAATGATAATGAAGGTATAGAAGTTAATAACTCTAATGATAATGAAGTTATAGAAGTTGATAACTCTAATGATGATGAAGCACGTTGGAAGCCTGAAACAGGGATGTGTTTTTCTTGCATAGATGATGTTAAAACATTTTATGGAGAGTATGCTTTGAAAAAGGGTTTTAGGTGGAAGATTAGAACATCAAGAAAAGGAGATAATGGAGAGATCTGCTATGTAATTCTTGCTTGCTCAAGAGAGGGATCTAGATTGTCAAAAAGTTCGTTAAAAACTCTCCCATCTAAGGAAAAAAATTGTCCAGCCAAGATTTGCATTAAGTTGGAAGACGATGGTTTGTGGTACATTAAAAGATTTGAATCTAGCCATTCTCACGAGACTAGCCCTGCAAAGGGAAGATTGTTCAAGGCTAACAGGAAAATGAACTTGCGTGTAAAGAGAACAATCCAAAGCACTGATGATCCAGGAGTGAGGATCAAGAAGACTTTTCAACCTCTTGTTAAAGATGTAGGAGCGCATGAAATTACACCATTTTGCAAACGAGATGCGGGAAATTATGTTTACAAGGAACGACATGCAATTGGAAAAGAAGACAACAACACAAGAATGATGGAACTCGAAGCTGAAGTGAAGCGAATAAGTGAAAAAATGGAGAAGTACCACTCAGAGCTGCTAGAAAAGATGGAGTGGATGAATGTCAAGATCAATCGAATCACAGATGCCTTTGGTACACTTCAAGGGTATGCGCATGGTACTCAATCACAAGTTAAACCAACCAAACAACAGCTTTCTTGTATCCTTGAGTTTGGCGGTGTATCCATGGGGGTTGAGTATGGTGATCTAAACATTAATCGTGGACCAGCAGGTGCAGGAGCTTTACTGCTTGCTGAAGATGGGAGTCTGCTCTATGGGTTTCGCCAAGGACTGGGTCACCAAACAAAAGAGTCCGCTGAGTATCGGGCTTTACTCTTAGGATTGAAACATGCGAGTATGAAAGGATTTAAGTATGTTACTGCCAAAGGAGACTCGGAACTTGTTATCAATCAGATTCTGGATCCCTGGAAAATCAAAGACGAACATTTAAAGAAATTATGTGCTGAGGCTTTGGAGCTGAGTGATAACTTTCATTCATTCCGCATCCAACACATTTCCAGGGAACGTAACTATGGAGCTGTTCGTAATGCAAACCGGGCCATCAATCTCAACGGTTAG

Protein sequence

>MsaT030090.1

MDSSKNLVDCGTIHDEGIEVDNSNDNEGIEVDNSNDNEGIEVDNSNDNEGIEVNNSNDNEVIEVDNSNDDEARWKPETGMCFSCIDDVKTFYGEYALKKGFRWKIRTSRKGDNGEICYVILACSREGSRLSKSSLKTLPSKEKNCPAKICIKLEDDGLWYIKRFESSHSHETSPAKGRLFKANRKMNLRVKRTIQSTDDPGVRIKKTFQPLVKDVGAHEITPFCKRDAGNYVYKERHAIGKEDNNTRMMELEAEVKRISEKMEKYHSELLEKMEWMNVKINRITDAFGTLQGYAHGTQSQVKPTKQQLSCILEFGGVSMGVEYGDLNINRGPAGAGALLLAEDGSLLYGFRQGLGHQTKESAEYRALLLGLKHASMKGFKYVTAKGDSELVINQILDPWKIKDEHLKKLCAEALELSDNFHSFRIQHISRERNYGAVRNANRAINLNG*