Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG030105 | XP_013450862.1 | 94.536 | 1574 | 59 | 3 | 1 | 1547 | 16 | 1589 | 0.0 | 3021 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG030105 | tr|A0A072U5H4|A0A072U5H4_MEDTR | 94.536 | 1574 | 59 | 3 | 1 | 1547 | 16 | 1589 | 0.0 | 3021 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG030105 | MtrunA17_Chr6g0451341 | 94.536 | 1574 | 59 | 3 | 1 | 1547 | 16 | 1589 | 0.0 | 2944 |
| MsaG030105 | MtrunA17_Chr8g0386831 | 34.014 | 147 | 91 | 4 | 24 | 164 | 99 | 245 | 3.50e-17 | 87.0 |
| MsaG030105 | MtrunA17_Chr5g0397411 | 33.333 | 141 | 90 | 3 | 24 | 160 | 96 | 236 | 3.42e-16 | 84.0 |
| MsaG030105 | MtrunA17_Chr6g0474401 | 32.331 | 133 | 86 | 3 | 32 | 160 | 12 | 144 | 4.81e-14 | 74.3 |
| MsaG030105 | MtrunA17_Chr1g0156791 | 34.667 | 150 | 90 | 5 | 239 | 382 | 1 | 148 | 1.54e-13 | 76.3 |
| MsaG030105 | MtrunA17_Chr8g0386841 | 34.043 | 141 | 89 | 3 | 24 | 160 | 77 | 217 | 9.55e-11 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG030105 | AT3G48050.1 | 51.629 | 798 | 339 | 18 | 23 | 790 | 46 | 826 | 0.0 | 658 |
| MsaG030105 | AT3G48050.2 | 51.629 | 798 | 339 | 18 | 23 | 790 | 46 | 826 | 0.0 | 658 |
| MsaG030105 | AT3G48060.1 | 51.189 | 799 | 341 | 19 | 23 | 790 | 46 | 826 | 0.0 | 646 |
| MsaG030105 | AT3G48060.1 | 38.715 | 576 | 269 | 21 | 1028 | 1534 | 1046 | 1606 | 1.94e-84 | 306 |
| MsaG030105 | AT3G48060.3 | 51.189 | 799 | 341 | 19 | 23 | 790 | 46 | 826 | 0.0 | 646 |
| MsaG030105 | AT3G48060.3 | 38.715 | 576 | 269 | 21 | 1028 | 1534 | 1046 | 1606 | 1.94e-84 | 306 |
| MsaG030105 | AT3G48060.2 | 51.064 | 799 | 342 | 19 | 23 | 790 | 47 | 827 | 0.0 | 645 |
| MsaG030105 | AT3G48060.2 | 38.715 | 576 | 269 | 21 | 1028 | 1534 | 1047 | 1607 | 1.81e-84 | 306 |
| MsaG030105 | AT4G11560.1 | 36.667 | 120 | 73 | 2 | 44 | 160 | 148 | 267 | 8.37e-16 | 83.2 |
| MsaG030105 | AT4G11560.2 | 36.220 | 127 | 76 | 3 | 39 | 160 | 141 | 267 | 9.21e-16 | 82.8 |
Find 427 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAACCAGTTCTTCAAC+TGG | 0.416688 | 6:+2516684 | MsaT030105.1:CDS |
| AAAAGGAAGGAACCTGATGG+CGG | 0.689775 | 6:+2519248 | MsaT030105.1:CDS |
| AAAATGGGGAAGTCAGAGTT+TGG | 0.408671 | 6:+2519085 | MsaT030105.1:CDS |
| AAACAGCCTTCATTATCCAG+TGG | 0.636195 | 6:+2518465 | MsaT030105.1:CDS |
| AAACGGTGACTACAGTGGTC+GGG | 0.565568 | 6:-2513253 | None:intergenic |
| AAAGAGAGAGCGAGGAGATC+AGG | 0.345060 | 6:+2515231 | MsaT030105.1:CDS |
| AAAGGAGGCGAGTCCTGGGG+AGG | 0.646644 | 6:-2514637 | None:intergenic |
| AAAGGAGTCTCATGCAGAGG+CGG | 0.538075 | 6:+2517232 | MsaT030105.1:CDS |
| AAAGGTGTTGAACTTCCTTC+AGG | 0.354723 | 6:+2514862 | MsaT030105.1:CDS |
| AAAGTCGCATTTCTCCGTAA+AGG | 0.569245 | 6:+2514844 | MsaT030105.1:CDS |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 2513233 | 2519322 | 2513233 | ID=MsaG030105 |
| Chr6 | mRNA | 2513233 | 2519322 | 2513233 | ID=MsaT030105.1;Parent=MsaG030105 |
| Chr6 | exon | 2513233 | 2513301 | 2513233 | ID=MsaT030105.1.exon1;Parent=MsaT030105.1 |
| Chr6 | CDS | 2513233 | 2513301 | 2513233 | ID=cds.MsaT030105.1;Parent=MsaT030105.1 |
| Chr6 | exon | 2514595 | 2514966 | 2514595 | ID=MsaT030105.1.exon2;Parent=MsaT030105.1 |
| Chr6 | CDS | 2514595 | 2514966 | 2514595 | ID=cds.MsaT030105.1;Parent=MsaT030105.1 |
| Chr6 | exon | 2515052 | 2518140 | 2515052 | ID=MsaT030105.1.exon3;Parent=MsaT030105.1 |
| Chr6 | CDS | 2515052 | 2518140 | 2515052 | ID=cds.MsaT030105.1;Parent=MsaT030105.1 |
| Chr6 | exon | 2518177 | 2518313 | 2518177 | ID=MsaT030105.1.exon4;Parent=MsaT030105.1 |
| Chr6 | CDS | 2518177 | 2518313 | 2518177 | ID=cds.MsaT030105.1;Parent=MsaT030105.1 |
| Chr6 | exon | 2518346 | 2519322 | 2518346 | ID=MsaT030105.1.exon5;Parent=MsaT030105.1 |
| Chr6 | CDS | 2518346 | 2519322 | 2518346 | ID=cds.MsaT030105.1;Parent=MsaT030105.1 |
| Gene Sequence |
>MsaT030105.1 ATGTGGCCTGTACCTTCTAACCCGACCACTGTAGTCACCGTTTCTTCACCGTCAAAATTTATATGCAAAGATGGACGCAAGATTCGTGTTGGTGACTGTGCCCTATTCAAACCTCCCCAGGACTCGCCTCCTTTTATTGGAATAATTCGCAAGTTAATATTTAATAAAGAAGAAAGTCCAAGTTTAGAAGTGAATTGGTTTTACCGACCTGCTGATTTGAAGCTCTCTAAAGGGATTGCAGTGGAAGCTGCACCAAACGAAGTCTTCTACTCCTTTCACAAGGATGAGACACATGCTGCATCATTACTCCATCCTTGTAAAGTCGCATTTCTCCGTAAAGGTGTTGAACTTCCTTCAGGGATATCCGCATTTGTTTGTAGACGAGTGTACGACATTGACAACAATTGTTTACGGTGGCTAACTGACAAGGACTTCGTTAATGATCAGCAGGAAGAAGTTGACCAACTATTAGACAAGACAAAACTAGAAATGCATGGGGCTGTGCAGTCAGGAGGCCGTTCTCCAAAACCCTTGAATGGTCCAACGTCAACACAGTCATTGAAATCTAGTTCTGATAACATTCAAAATAGTTCTTCCTTTGGTGTACAGGGTAAGGGAAAAAAGAGAGAGCGAGGAGATCAGGGATCTGATTCATCTAAAAAAGAGCGATTATTTAAGGTAGAAGTTGGAGATTCTGGTCAGTTTAGGCTGGAGATGCTAAGGACCGAGATTGCTAGAATCACTGAAAAAGGCGGGCTTGTGGACTTTGAAGGAGTTGAAAAATTTGTACAGCTCATGCAACCTGATAGTGCTGATAAGAAAATTGATTTGGCTGGGAGAACGATGCTTGTTGACGTCATATCTCTCACGGAACATTATGACTGTCTTGGCTGGTTTGTACAGCTTCGTGGTTTGCCTGTGTTAGATGAATGGCTACAAGAGGTCCATAAGGGGAAAATTGGCGATGGTAATGTTGATAAGTCTGAAGAGTTTTTGTTAGCTTTACTCCGTGCATTGGATAAGCTTCCTGTGAACCTTCATGCACTACAAACCTGCAATGTTGGAAAATCTGTTAATAATTTACGCAGCCACAAAAATTCTGAAATTCAGAGGAAAGCCAGGAGTTTGGTAGACACATGGAAAAAGCGTGTTGAAGCTGAAATGAATATGACTGATTCAAAACCTGGTTCAACCCGTGCTGTTTCTTGGCCCACAAAGCCATCACCGTCAGATGTTTCTCACTCTGGAAATAGGAGAACCGGAGGATCCTCTGAGAATGTTGCGAAGAGCTCTCCTATTCAACCTTCAGCATCCAAAAATTCCCAATCTAAACATAATTCTGGAGAAGTACTATCCAAGTTCTCTTCATCCCCTGGCTCAACTAAATCAATGACTGCCTCAGTAGGCAGCAACACAAAAGATCAAAATGTTAAACTCATTGTTGGTGCAACGACATCAGATCTACCTCTGACACCTATCAAGGAGGAGAGGAGCAGCAGTTCTAGTCAATCTCAAAACAACAGCAGATCTTGTTCTAGTGAACATGCGAAGAGCTCCACTGCTGGGTCAATAAGTGGGAGCAAAGTTCCTGGTAGTGCATCCCGATCTCGAAAGTCGAGTAATGGTATACATGCAGCTGGCGTTGCTGTAGTTCTGAAGGATCATAGCTCTGCCAAGAATTCAACAAGAAATTCATCTTCTGATAAAGTTTCACCGACCAGAATGTCCCATGAAAAACCATCTGATCAGCCCCTTACTGATCAAGGGCATAATCAGCGATTGATTGTGAGGTTGCCGAACACTGGGCGCAGTCCTTCCAGGGGAGCTAGTGGGGGCACTTTTGAAGAACCTGCTATTACATGTGGCAAAACATCACCTCCTACCGACAAGAATGATAATCAGGATAGGAGAGTTAAAGCTAAAACTGATTGTTTGCAAACCCATGTGTCGAATGTGATGAATGATGCATCCAATGCTAAGGAAATTACTGGTTGTGATGATGCTAAGTGCTCTCCACGAGTGGATGTACGGTGCAGGGCTAATGAAGATGGTGATAAAGTAGCAGAAACGTCAAAAACAACCAGTTCTTCAACTGGATTTGTTTCCAGATCAGGGCAGACTTATGATGCTTCTTTAAGCCCCATGAATGCATTAGTTGAGAGCTGTGTGAAATTTTCCGAAGCAAGTTCCTCTGTGTCCCCTGGAGATGATGGTATGAATCTGCTTGCTTCTGTGGCTGCTGGAGAAATGTCTAGATGTGAAAATGTCTCACCTCTGCCTTCACCCGAGAGGAAGTCTCCTGCAGCTGATGAATCAAGCTCAGGGAATGATCGACATTCTTTTGAAGCAGCTGGTCGCACGCAGAGACAGTCTGATGGCGGGGCTACCGGAGAGCATCCTGTGAATACTTTGCAATTCAAAAATAATTCTAGGCATCTGGTGACTTCAGTATCGCGTGATTTTTCAGATGGTGAAGCCGTTTCATCTAGCTGTGTGGAGAAAACTGGTGATGGTAAAACTCAATTAAATTTCTCTACTACGGACACGATACAAAATGCTGGAGGTCCATGCTTACGGCCTGACACCAAAGAGGATACGTCTGAAACTGTATTTCCAGGCAGAAAGGAGTCTCATGCAGAGGCGGGAGGCGCAGAGGGTTTTCACGAGAGGAGGGAACTGGGAACTCAATGGCCTAAAAGCAGCAGTAGTCCGGGCTCTAAAAAATCGAAGACTTCCTCTTTTGATGATGATCAGAAGACTGATAACATGGATGGCGGTCTCACAGAGCACGGGAAAATGTTAGTTTCAGAAACCGTTGCAAGTGGTATGATTGAAAATGAACCAGGTAAAAAATCCCCCGAGTTATCTTCAGGTGTAGATAGTAAGAGCCAGTTCAGTGCGGAGAAAATGATGGGTAGTGTGCCAATGCAAAAAGGCTCTCCAGTTGCTGATACCTGTGAGTCCATAGATGTGAAAAGGGAGGATGTTATGTTACCCGCTTCTGGTAGTGCTTTGAAAAGGGAGGATGTTGCTAATAATGTTATGGCAGTTGAGATCAAGCCTGCTGAGAAACAAATGGATCTGGATTCAGCAGTTGCAGATGGTGTTGATGAGCGTTGTGAAGGGAATTCTGTTAAAAAAGAGTTCATTGGCGGTTCATCTGTTCCCTCAGATATACCAATTACTTCTGAAAAGGAGAGCGACGTGCCCGAAACATGTGATTCTAATATAGAAGGAAAGAAATCTGTAGTTTCTGCAGAGTTGAATGCGGTCAACACTAATCCATCCACAATTGCTAGTGGTTCTGATGCAGCTGTAAAACTGGACTTTGATTTAAATGAGGGATACCCAGTTGAAGATGCAGGCCAAGGGGATATTGTTAGACAGGAAGATCCTACCAGTTCATCTGCAGTTCATGTTCCTTGCCCATTGCCTTTTCCTATTCCATCCCTCTCTGGGGCCTTCCATGCTTCTATTACAGTGGCTTCTGCTACCAAAGGACCAGTTGTTCTACCAGAAAATCCATTGGATGCATTCCGGCCAGCGGAACCTAGGAAAAATGCTGAGATGCCATCAAATACTGGTGATATTCCTTCCGTTGAAACAACATCAGTCAAACAAGGCCGTGCTCCTTTAGATTTTGATTTAAATGTGGCAGATGAAATAGAGTCTGGAGCACATGACCGCAGTGCTGTGGGACTTGATCTTGATCTTAACAGGTTAGATGAGACTCCTGAAGCTGGATCTTTCTCTATGGGTAAAATGGATATTCCTTCTTTACCAAGTAAACAGCCTTCATTATCCAGTGGGTTATCTAATGGCGGAAGTGTCTCAAGAGACTTTGATTTAAATAATGGGCCAGGCCTTGACGAAGTTAGCACTGAAGTCCCTGCTCGTAGTCTGCAGATGAAAGGTGCTGTGCCATTTTCAAATTCTGTTCATGGCACCAGAACAAATAACGCTGAATTTGGGAACTATTCTTCATGGTTTCCTCCAGGTAATTCGTATTCTGCAATTACTGTCCCACCACTTCTGCCTGGTAGAGGGGAACAGAGCTATGTTGGTGCTTCTGGACCACAGCGGATCATAGGTTCGACAGGTAGCTCACCCTTTAGTCCTGAAATGTACAGAGGGCCAGTACTTTCATCCTCTCCTGCTGTTGCATATCCTCCTACAACCCCCTTTCCATATCCGGGATTTCCATTTGAAACCAATTTTCCATTATCTTCAAACGCCTTTTCTGGTTGTTCAACACCATTTATGGATTCATCAACTGTTGGTGGACTTTGCTTTCCTACTATGCCCTCGCAGCCAGTTGGGCCTGGAGGCATTGTTTCATCTACATATCCCCGTCCTTATGTTATGAGTCTTCCAGGCAGTACAAGTAACGTAATTCCCGACAGCAGAAAATGGGGAAGTCAGAGTTTGGATCTTAATTCAGGCCCTGGTGGTACAGACGTCGAGCGCAGAGACGACAGATTGCCTTCAGGGTTGAGGCAGATGCCTGTCCCCAGTCCACAAGCCTTGATGGAGGATCACTTGAAGATGTTTCAGATGGCCGGTGCATTAAAAAGGAAGGAACCTGATGGCGGTTGGGATGGAACTGATAGATTCAGTTACAAACATCCCCCCTCATGGCAGTAG |
| Protein sequence |
>MsaT030105.1 MWPVPSNPTTVVTVSSPSKFICKDGRKIRVGDCALFKPPQDSPPFIGIIRKLIFNKEESPSLEVNWFYRPADLKLSKGIAVEAAPNEVFYSFHKDETHAASLLHPCKVAFLRKGVELPSGISAFVCRRVYDIDNNCLRWLTDKDFVNDQQEEVDQLLDKTKLEMHGAVQSGGRSPKPLNGPTSTQSLKSSSDNIQNSSSFGVQGKGKKRERGDQGSDSSKKERLFKVEVGDSGQFRLEMLRTEIARITEKGGLVDFEGVEKFVQLMQPDSADKKIDLAGRTMLVDVISLTEHYDCLGWFVQLRGLPVLDEWLQEVHKGKIGDGNVDKSEEFLLALLRALDKLPVNLHALQTCNVGKSVNNLRSHKNSEIQRKARSLVDTWKKRVEAEMNMTDSKPGSTRAVSWPTKPSPSDVSHSGNRRTGGSSENVAKSSPIQPSASKNSQSKHNSGEVLSKFSSSPGSTKSMTASVGSNTKDQNVKLIVGATTSDLPLTPIKEERSSSSSQSQNNSRSCSSEHAKSSTAGSISGSKVPGSASRSRKSSNGIHAAGVAVVLKDHSSAKNSTRNSSSDKVSPTRMSHEKPSDQPLTDQGHNQRLIVRLPNTGRSPSRGASGGTFEEPAITCGKTSPPTDKNDNQDRRVKAKTDCLQTHVSNVMNDASNAKEITGCDDAKCSPRVDVRCRANEDGDKVAETSKTTSSSTGFVSRSGQTYDASLSPMNALVESCVKFSEASSSVSPGDDGMNLLASVAAGEMSRCENVSPLPSPERKSPAADESSSGNDRHSFEAAGRTQRQSDGGATGEHPVNTLQFKNNSRHLVTSVSRDFSDGEAVSSSCVEKTGDGKTQLNFSTTDTIQNAGGPCLRPDTKEDTSETVFPGRKESHAEAGGAEGFHERRELGTQWPKSSSSPGSKKSKTSSFDDDQKTDNMDGGLTEHGKMLVSETVASGMIENEPGKKSPELSSGVDSKSQFSAEKMMGSVPMQKGSPVADTCESIDVKREDVMLPASGSALKREDVANNVMAVEIKPAEKQMDLDSAVADGVDERCEGNSVKKEFIGGSSVPSDIPITSEKESDVPETCDSNIEGKKSVVSAELNAVNTNPSTIASGSDAAVKLDFDLNEGYPVEDAGQGDIVRQEDPTSSSAVHVPCPLPFPIPSLSGAFHASITVASATKGPVVLPENPLDAFRPAEPRKNAEMPSNTGDIPSVETTSVKQGRAPLDFDLNVADEIESGAHDRSAVGLDLDLNRLDETPEAGSFSMGKMDIPSLPSKQPSLSSGLSNGGSVSRDFDLNNGPGLDEVSTEVPARSLQMKGAVPFSNSVHGTRTNNAEFGNYSSWFPPGNSYSAITVPPLLPGRGEQSYVGASGPQRIIGSTGSSPFSPEMYRGPVLSSSPAVAYPPTTPFPYPGFPFETNFPLSSNAFSGCSTPFMDSSTVGGLCFPTMPSQPVGPGGIVSSTYPRPYVMSLPGSTSNVIPDSRKWGSQSLDLNSGPGGTDVERRDDRLPSGLRQMPVPSPQALMEDHLKMFQMAGALKRKEPDGGWDGTDRFSYKHPPSWQ* |
