AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG030139

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030139 MtrunA17_Chr6g0451001 95.035 141 2 3 1 140 1 137 3.36e-84 268
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030139 AT3G01460.1 36.957 138 67 7 7 132 4 133 6.87e-14 68.2

Find 61 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTCCTGCGCCTCTTCC+TGG 0.258264 6:+3112677 MsaT030139.1:CDS
TAAGTCCGGCGCCGGTGGTT+TGG 0.372683 6:+3112727 MsaT030139.1:CDS
GTTACCTGCGGCGGTGGAAT+CGG 0.405682 6:-3112483 None:intergenic
AGGAAGAGGCGCAGGACCAC+CGG 0.435115 6:-3112675 None:intergenic
GGAGTTCGTGGTGGAAGAAT+TGG 0.435748 6:+3112791 MsaT030139.1:CDS
GGACCACCGGGAGGAGGAGC+GGG 0.442926 6:-3112662 None:intergenic
GTGGTTGTTGCGGCGGCGGA+AGG 0.454858 6:-3112508 None:intergenic
AGAATTGGAGCGGCGAGTAT+AGG 0.461015 6:+3112806 MsaT030139.1:CDS
GGCGCAGGACCACCGGGAGG+AGG 0.465429 6:-3112668 None:intergenic
AAGGTTGTTACCTGCGGCGG+TGG 0.470430 6:-3112489 None:intergenic
ACGATGTCGACGGTGAAGTC+AGG 0.471202 6:-3112620 None:intergenic
TGCGCTGTTTGTTCTAAGTC+CGG 0.471685 6:+3112713 MsaT030139.1:CDS
GGTGTGTGGTGAGTGTTTAG+AGG 0.473513 6:+3112835 MsaT030139.1:CDS
GAAGTGACACCGCCAGGAAG+AGG 0.473868 6:-3112689 None:intergenic
GCGCAGGAAGTGACACCGCC+AGG 0.487448 6:-3112695 None:intergenic
AAGAAGAAGGTGAAGGTGAA+GGG 0.487631 6:-3112580 None:intergenic
GGAAGAGGCGCAGGACCACC+GGG 0.493728 6:-3112674 None:intergenic
GAGAATCCCGCTCCTCCTCC+CGG 0.498098 6:+3112656 MsaT030139.1:CDS
GTTTGTTCTAAGTCCGGCGC+CGG 0.499686 6:+3112719 MsaT030139.1:CDS
TGAGGTGGTTGTTGCGGCGG+CGG 0.499971 6:-3112512 None:intergenic
AGGACCACCGGGAGGAGGAG+CGG 0.509301 6:-3112663 None:intergenic
GAAGAAGAAGGTGAAGGTGA+AGG 0.514948 6:-3112581 None:intergenic
CTAACTCACTCCGATGATCA+TGG 0.522517 6:+3112451 None:intergenic
TTGGCGTGTGCTGGAGTTCG+TGG 0.522800 6:+3112779 MsaT030139.1:CDS
GTTAGTTCCGCCATGATCAT+CGG 0.526042 6:-3112461 None:intergenic
TGTTCTAAGTCCGGCGCCGG+TGG 0.533415 6:+3112722 MsaT030139.1:CDS
ACACCGCCAGGAAGAGGCGC+AGG 0.541866 6:-3112683 None:intergenic
GTGTGTGGTGAGTGTTTAGA+GGG 0.544832 6:+3112836 MsaT030139.1:CDS
CGCGTGTCAATTTGATGAGG+TGG 0.545133 6:-3112527 None:intergenic
TGTTTACTCTTCACACCCAA+CGG 0.559421 6:-3112877 None:intergenic
AAGGTGAAGGGATCTCGTTA+AGG 0.562146 6:-3112568 None:intergenic
ATTTGATGAGGTGGTTGTTG+CGG 0.564670 6:-3112518 None:intergenic
GCCGGTGGTTTGGTGTGTGA+TGG 0.576668 6:+3112737 MsaT030139.1:CDS
TCCATCACACACCAAACCAC+CGG 0.577419 6:-3112738 None:intergenic
TGTCGACGGTGAAGTCAGGT+AGG 0.582244 6:-3112616 None:intergenic
ACACACCAAACCACCGGCGC+CGG 0.584128 6:-3112732 None:intergenic
GAGGGTGTGAAAAGTAAAGA+TGG 0.595817 6:+3112854 MsaT030139.1:CDS
GAAAAGTAAAGATGGCCGTT+GGG 0.597829 6:+3112862 MsaT030139.1:CDS
CGGCGGAAGGTTGTTACCTG+CGG 0.600798 6:-3112495 None:intergenic
TGAAAAGTAAAGATGGCCGT+TGG 0.601270 6:+3112861 MsaT030139.1:CDS
GTCAGGTAGGGTTTCAACGA+AGG 0.601624 6:-3112603 None:intergenic
GAGCGGCGAGTATAGGAGAG+TGG 0.606273 6:+3112813 MsaT030139.1:CDS
AGCGGCGAGTATAGGAGAGT+GGG 0.615377 6:+3112814 MsaT030139.1:CDS
TGATGAGGTGGTTGTTGCGG+CGG 0.616771 6:-3112515 None:intergenic
CGGAAGGTTGTTACCTGCGG+CGG 0.621332 6:-3112492 None:intergenic
TGGTGTGTGATGGATGTGAA+CGG 0.622459 6:+3112747 MsaT030139.1:CDS
TCTCGTTAAGGTCGATGCAG+AGG 0.625670 6:-3112556 None:intergenic
GTCGACGGTGAAGTCAGGTA+GGG 0.629490 6:-3112615 None:intergenic
GGACTTAGAACAAACAGCGC+AGG 0.633289 6:-3112711 None:intergenic
GTTTCAACGAAGGAAGAAGA+AGG 0.638653 6:-3112593 None:intergenic
AGTATAGGAGAGTGGGTGTG+TGG 0.639379 6:+3112821 MsaT030139.1:CDS
CTAACCGATTCCACCGCCGC+AGG 0.641238 6:+3112479 MsaT030139.1:CDS
AGAGGCGCAGGACCACCGGG+AGG 0.666010 6:-3112671 None:intergenic
ACGAAGGAAGAAGAAGGTGA+AGG 0.668161 6:-3112587 None:intergenic
GCGTGTGCTGGAGTTCGTGG+TGG 0.673316 6:+3112782 MsaT030139.1:CDS
AATCCCGCTCCTCCTCCCGG+TGG 0.675488 6:+3112659 MsaT030139.1:CDS
GGTCCTGCGCCTCTTCCTGG+CGG 0.686107 6:+3112680 MsaT030139.1:CDS
TCGTGGTGGAAGAATTGGAG+CGG 0.690037 6:+3112796 MsaT030139.1:CDS
GAACGCGTGTCAATTTGATG+AGG 0.700418 6:-3112530 None:intergenic
ATAAGTACGAACGATGTCGA+CGG 0.701679 6:-3112630 None:intergenic
ACTCACTCCGATGATCATGG+CGG 0.776957 6:+3112454 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 3112464 3112889 3112464 ID=MsaG030139
Chr6 mRNA 3112464 3112889 3112464 ID=MsaT030139.1;Parent=MsaG030139
Chr6 exon 3112464 3112889 3112464 ID=MsaT030139.1.exon1;Parent=MsaT030139.1
Chr6 CDS 3112464 3112889 3112464 ID=cds.MsaT030139.1;Parent=MsaT030139.1
Gene Sequence

>MsaT030139.1

ATGATCATGGCGGAACTAACCGATTCCACCGCCGCAGGTAACAACCTTCCGCCGCCGCAACAACCACCTCATCAAATTGACACGCGTTCAATCCTCTGCATCGACCTTAACGAGATCCCTTCACCTTCACCTTCTTCTTCCTTCGTTGAAACCCTACCTGACTTCACCGTCGACATCGTTCGTACTTATCACGAGAATCCCGCTCCTCCTCCCGGTGGTCCTGCGCCTCTTCCTGGCGGTGTCACTTCCTGCGCTGTTTGTTCTAAGTCCGGCGCCGGTGGTTTGGTGTGTGATGGATGTGAACGGAGTTTTCATTTGGCGTGTGCTGGAGTTCGTGGTGGAAGAATTGGAGCGGCGAGTATAGGAGAGTGGGTGTGTGGTGAGTGTTTAGAGGGTGTGAAAAGTAAAGATGGCCGTTGGGTGTGA
Protein sequence

>MsaT030139.1

MIMAELTDSTAAGNNLPPPQQPPHQIDTRSILCIDLNEIPSPSPSSSFVETLPDFTVDIVRTYHENPAPPPGGPAPLPGGVTSCAVCSKSGAGGLVCDGCERSFHLACAGVRGGRIGAASIGEWVCGECLEGVKSKDGRWV*