AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG030141

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030141 MtrunA17_Chr6g0450991 98.958 288 2 1 1 288 1 287 0.0 583
MsaG030141 MtrunA17_Chr5g0415941 43.621 243 91 8 30 232 39 275 8.54e-46 157
MsaG030141 MtrunA17_Chr4g0060031 42.922 219 92 5 40 229 64 278 5.38e-44 152
MsaG030141 MtrunA17_Chr8g0362751 55.944 143 56 3 29 164 43 185 2.22e-42 147
MsaG030141 MtrunA17_Chr8g0362151 64.948 97 34 0 67 163 76 172 5.76e-39 138
MsaG030141 MtrunA17_Chr8g0381741 52.113 142 55 1 33 174 32 160 1.01e-38 138
MsaG030141 MtrunA17_Chr6g0453021 62.162 111 41 1 53 163 82 191 2.16e-38 136
MsaG030141 MtrunA17_Chr5g0416391 57.031 128 47 1 44 163 45 172 4.92e-38 135
MsaG030141 MtrunA17_Chr3g0123691 62.264 106 40 0 67 172 59 164 1.07e-36 132
MsaG030141 MtrunA17_Chr3g0119291 59.664 119 46 1 48 164 37 155 1.65e-36 130
MsaG030141 MtrunA17_Chr7g0265521 58.824 102 35 1 67 168 62 156 4.46e-32 118
MsaG030141 MtrunA17_Chr7g0217111 56.250 112 45 2 53 163 67 175 3.11e-31 117
MsaG030141 MtrunA17_Chr1g0179581 59.341 91 30 1 67 157 27 110 1.92e-29 108
MsaG030141 MtrunA17_Chr8g0346251 54.255 94 43 0 72 165 45 138 3.50e-29 111
MsaG030141 MtrunA17_Chr3g0127511 57.143 91 39 0 70 160 40 130 1.10e-28 110
MsaG030141 MtrunA17_Chr5g0403691 47.748 111 56 1 68 176 53 163 7.76e-23 94.0
MsaG030141 MtrunA17_Chr5g0403701 48.182 110 48 2 68 175 53 155 2.48e-22 92.0
MsaG030141 MtrunA17_Chr4g0055851 48.148 108 47 2 53 160 107 205 3.93e-21 90.5
MsaG030141 MtrunA17_Chr1g0151971 50.000 104 45 1 65 168 127 223 4.73e-21 90.5
MsaG030141 MtrunA17_Chr4g0044091 43.089 123 58 3 65 187 62 172 1.07e-19 84.7
MsaG030141 MtrunA17_Chr5g0399491 40.260 154 88 3 55 206 125 276 1.36e-19 86.7
MsaG030141 MtrunA17_Chr4g0054421 50.538 93 39 1 68 160 127 212 2.39e-19 85.5
MsaG030141 MtrunA17_Chr8g0335131 50.538 93 39 1 68 160 146 231 2.96e-19 85.9
MsaG030141 MtrunA17_Chr7g0258831 42.500 120 69 0 62 181 154 273 4.74e-19 85.1
MsaG030141 MtrunA17_Chr5g0400781 49.462 93 40 1 68 160 123 208 1.57e-18 83.2
MsaG030141 MtrunA17_Chr2g0308611 42.105 114 66 0 68 181 200 313 3.26e-18 83.2
MsaG030141 MtrunA17_Chr3g0135331 42.593 108 61 1 68 175 145 251 6.57e-18 82.0
MsaG030141 MtrunA17_Chr1g0175651 40.833 120 71 0 62 181 148 267 9.23e-18 81.6
MsaG030141 MtrunA17_Chr4g0071411 47.778 90 40 1 71 160 91 173 1.62e-17 79.3
MsaG030141 MtrunA17_Chr2g0299031 43.011 93 46 1 68 160 82 167 1.88e-13 68.2
MsaG030141 MtrunA17_Chr8g0350321 32.558 129 63 4 57 183 105 211 9.21e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030141 AT3G01470.1 57.143 287 101 8 1 282 1 270 1.28e-97 288
MsaG030141 AT5G65310.2 60.909 110 43 0 65 174 51 160 2.11e-38 137
MsaG030141 AT5G65310.1 62.037 108 41 0 67 174 71 178 3.13e-38 137
MsaG030141 AT2G22430.1 68.367 98 31 0 67 164 61 158 3.70e-38 137
MsaG030141 AT4G40060.1 49.315 146 67 1 54 192 45 190 5.02e-38 136
MsaG030141 AT1G69780.1 50.355 141 62 2 57 189 72 212 6.97e-38 135
MsaG030141 AT5G15150.1 70.213 94 28 0 67 160 114 207 1.17e-37 135
MsaG030141 AT3G01220.1 65.979 97 33 0 67 163 86 182 1.76e-35 129
MsaG030141 AT1G26960.1 46.622 148 71 2 29 174 36 177 2.84e-33 122
MsaG030141 AT1G27045.5 62.000 100 38 0 68 167 25 124 7.01e-33 119
MsaG030141 AT1G27045.3 62.000 100 38 0 68 167 25 124 7.01e-33 119
MsaG030141 AT1G27045.2 62.000 100 38 0 68 167 17 116 7.34e-33 119
MsaG030141 AT1G27045.1 62.000 100 38 0 68 167 68 167 8.82e-33 120
MsaG030141 AT2G46680.1 51.961 102 49 0 69 170 33 134 5.89e-31 116
MsaG030141 AT1G27045.4 62.766 94 35 0 68 161 68 161 7.89e-31 116
MsaG030141 AT3G61890.1 53.684 95 44 0 71 165 33 127 2.14e-30 114
MsaG030141 AT2G46680.2 55.556 90 40 0 69 158 33 122 3.15e-29 112
MsaG030141 AT4G36740.1 56.701 97 35 1 68 164 55 144 1.02e-27 107
MsaG030141 AT4G36740.2 56.701 97 35 1 68 164 56 145 1.05e-27 107
MsaG030141 AT5G03790.2 55.914 93 34 1 68 160 77 162 5.24e-26 102
MsaG030141 AT5G03790.1 53.465 101 40 1 68 168 77 170 5.88e-26 102
MsaG030141 AT2G18550.1 54.639 97 37 1 68 164 61 150 6.85e-26 102
MsaG030141 AT5G66700.1 49.524 105 46 1 68 165 71 175 2.43e-24 98.6
MsaG030141 AT2G36610.1 45.902 122 43 3 67 180 70 176 3.25e-24 97.1
MsaG030141 AT5G53980.1 45.455 99 54 0 59 157 2 100 3.12e-20 85.5
MsaG030141 AT4G17460.1 47.115 104 48 1 57 160 124 220 4.25e-19 85.5
MsaG030141 AT2G22800.1 38.710 124 73 1 59 179 101 224 5.75e-19 84.7
MsaG030141 AT5G06710.1 42.500 120 69 0 62 181 184 303 7.84e-19 85.5
MsaG030141 AT2G01430.1 47.115 104 45 2 57 157 125 221 2.35e-18 83.2
MsaG030141 AT5G47370.1 44.348 115 52 2 51 160 108 215 2.38e-18 83.2
MsaG030141 AT4G37790.1 44.762 105 49 2 56 160 116 211 2.33e-17 80.5
MsaG030141 AT3G60390.1 47.917 96 43 1 65 160 159 247 3.87e-17 80.5
MsaG030141 AT2G44910.1 48.387 93 41 1 68 160 163 248 4.23e-17 80.5
MsaG030141 AT4G16780.1 46.237 93 43 1 68 160 129 214 4.98e-17 79.7
MsaG030141 AT2G01430.2 52.239 67 29 1 57 120 125 191 1.34e-14 71.6
MsaG030141 AT1G70920.1 46.237 93 43 1 68 160 69 154 3.62e-14 70.5
MsaG030141 AT1G70920.2 46.591 88 47 0 68 155 39 126 4.16e-14 69.3
MsaG030141 AT5G06710.4 56.897 58 25 0 62 119 184 241 3.75e-13 68.6
MsaG030141 AT1G73360.1 34.653 101 50 2 67 165 32 118 5.44e-11 63.5

Find 73 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTGAAGCTTCTCATTTA+AGG 0.097700 6:+3127584 None:intergenic
AACATGAACATGCTGTTTCT+TGG 0.235472 6:-3129856 MsaT030141.1:CDS
ATCCTAGTTTCTTGGCCAAC+TGG 0.259750 6:+3128416 None:intergenic
TCTGATTGAACTCTTTCAAT+AGG 0.332216 6:+3127342 None:intergenic
TTTCTTGGAAATGCTGATCT+TGG 0.335848 6:-3129841 MsaT030141.1:CDS
GTGGATGACCACATGAGTTC+TGG 0.344344 6:-3127474 MsaT030141.1:CDS
TCAGTGCCTCTCCCTCTTCA+TGG 0.347311 6:+3127247 None:intergenic
AGGTTGCAATCCTAGTTTCT+TGG 0.355925 6:+3128408 None:intergenic
AACATATGATTCCATTACTA+AGG 0.392206 6:-3128281 MsaT030141.1:CDS
GGAATCATATGTTGAAAGTA+GGG 0.419448 6:+3128291 None:intergenic
TTCAGAACCGTAGGGCTAGA+TGG 0.424104 6:-3128366 MsaT030141.1:CDS
GTGAGGCGGCGCTTCTTCTC+CGG 0.438471 6:+3129118 None:intergenic
GAAGATGATGGGAGTGATGA+TGG 0.439088 6:-3127318 MsaT030141.1:CDS
TGGAATCATATGTTGAAAGT+AGG 0.440148 6:+3128290 None:intergenic
TGTTGAAAGTAGGGAATCAT+AGG 0.441815 6:+3128300 None:intergenic
TTTCTCATTCTCCTTAGTAA+TGG 0.443693 6:+3128270 None:intergenic
CAAAGCTCTTCTCCAACATA+TGG 0.449623 6:+3128473 None:intergenic
AACCTAGGCAGGTGGCTGTA+TGG 0.452751 6:-3128390 MsaT030141.1:CDS
AAGAGGGAGAGGCACTGAAT+TGG 0.468712 6:-3127242 MsaT030141.1:CDS
TCCTAGTTTCTTGGCCAACT+GGG 0.472188 6:+3128417 None:intergenic
AGGGAGAGGCACTGAATTGG+TGG 0.476724 6:-3127239 MsaT030141.1:CDS
TCCTCATCATACAGTTCATC+CGG 0.477560 6:+3129160 None:intergenic
TGAGCTATGTCCACTGGAAG+TGG 0.478436 6:+3127513 None:intergenic
GTTCAATCAGAGGAAGATGA+TGG 0.478448 6:-3127330 MsaT030141.1:CDS
ACTAGGATTGCAACCTAGGC+AGG 0.478866 6:-3128401 MsaT030141.1:CDS
AGAAACTAGGATTGCAACCT+AGG 0.487565 6:-3128405 MsaT030141.1:CDS
CTGTATGGTTTCAGAACCGT+AGG 0.504508 6:-3128375 MsaT030141.1:CDS
CACATGAGTTCTGGAAGCGT+TGG 0.505249 6:-3127465 MsaT030141.1:CDS
TTTGATTCTTCTGCTTGTCG+TGG 0.507243 6:-3129883 MsaT030141.1:CDS
ACCCAGTTGGCCAAGAAACT+AGG 0.526542 6:-3128418 MsaT030141.1:CDS
AACAATGACACCAACGACAC+GGG 0.527406 6:+3127416 None:intergenic
TTGATTCTTCTGCTTGTCGT+GGG 0.527464 6:-3129882 MsaT030141.1:CDS
CATCCAGCATAGTTGTCAGC+TGG 0.530440 6:+3127372 None:intergenic
TTGTCTTCCATCTAGCCCTA+CGG 0.531725 6:+3128359 None:intergenic
TTTCCAGCTGACAACTATGC+TGG 0.536407 6:-3127375 MsaT030141.1:CDS
GCGCCGCCTCACTTCCGAGC+AGG 0.539627 6:-3129107 MsaT030141.1:intron
AAGATGATGGGAGTGATGAT+GGG 0.542417 6:-3127317 MsaT030141.1:CDS
GGAAGGTCAATGATGAGCAT+GGG 0.542750 6:-3129217 MsaT030141.1:CDS
TTCAATCAGAGGAAGATGAT+GGG 0.547251 6:-3127329 MsaT030141.1:CDS
AAAATCTGAGCTATGTCCAC+TGG 0.550742 6:+3127507 None:intergenic
AGGCAAGCAACCAACCTGCT+CGG 0.554902 6:+3129093 None:intergenic
AGGAAGGTCAATGATGAGCA+TGG 0.567797 6:-3129218 MsaT030141.1:CDS
GAGACCTTTCTTTAGCTCAC+CGG 0.569954 6:-3129179 MsaT030141.1:CDS
CAACAATGACACCAACGACA+CGG 0.575613 6:+3127415 None:intergenic
AACCATACAGCCACCTGCCT+AGG 0.579439 6:+3128388 None:intergenic
TATGTTGGAGAAGAGCTTTG+AGG 0.579800 6:-3128470 MsaT030141.1:CDS
GGGAGAGGCACTGAATTGGT+GGG 0.586237 6:-3127238 MsaT030141.1:CDS
TCATCCGGTGAGCTAAAGAA+AGG 0.588529 6:+3129175 None:intergenic
TGATGAATAGGTCCATATGT+TGG 0.590134 6:-3128485 MsaT030141.1:intron
AACCAACCTGCTCGGAAGTG+AGG 0.594175 6:+3129101 None:intergenic
ACATAACCATGAAGAGGGAG+AGG 0.603541 6:-3127253 MsaT030141.1:CDS
GAAAGCTGATCCACTTCCAG+TGG 0.611490 6:-3127523 MsaT030141.1:CDS
AGAACAAACTTGAGCCAGAG+AGG 0.615094 6:-3128444 MsaT030141.1:CDS
CAACCTGCTCGGAAGTGAGG+CGG 0.622855 6:+3129104 None:intergenic
GAAGGTCAATGATGAGCATG+GGG 0.623217 6:-3129216 MsaT030141.1:CDS
ACAATGACACCAACGACACG+GGG 0.624556 6:+3127417 None:intergenic
GCCAGAGAGGAAAACCCAGT+TGG 0.627216 6:-3128431 MsaT030141.1:CDS
TGAGCAACATAACCATGAAG+AGG 0.628182 6:-3127259 MsaT030141.1:CDS
GAATGAGAAACTCAAATCTG+AGG 0.629755 6:-3128257 MsaT030141.1:intron
GGAGAGGCACTGAATTGGTG+GGG 0.631899 6:-3127237 MsaT030141.1:CDS
AAGCGTTGGAAGTGCAGTGG+TGG 0.633588 6:-3127451 MsaT030141.1:CDS
CAACGCTTCCAGAACTCATG+TGG 0.634732 6:+3127466 None:intergenic
GAGCAACATAACCATGAAGA+GGG 0.640168 6:-3127258 MsaT030141.1:CDS
ACCGGATGAACTGTATGATG+AGG 0.641946 6:-3129161 MsaT030141.1:CDS
TGACAACTATGCTGGATGTG+TGG 0.645273 6:-3127367 MsaT030141.1:CDS
GTACTACGAAGAACAGTCAC+CGG 0.657008 6:-3129137 MsaT030141.1:CDS
AGGATTGCAACCTAGGCAGG+TGG 0.665303 6:-3128398 MsaT030141.1:CDS
TGGAAGCGTTGGAAGTGCAG+TGG 0.670661 6:-3127454 MsaT030141.1:CDS
ATGATGGGAGTGATGATGGG+AGG 0.679048 6:-3127314 MsaT030141.1:CDS
TATTGAAAGAGTTCAATCAG+AGG 0.686434 6:-3127340 MsaT030141.1:CDS
TGTATGGTTTCAGAACCGTA+GGG 0.689319 6:-3128374 MsaT030141.1:CDS
CAATGACACCAACGACACGG+GGG 0.756439 6:+3127418 None:intergenic
AAGGTCAATGATGAGCATGG+GGG 0.757009 6:-3129215 MsaT030141.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 3127215 3129926 3127215 ID=MsaG030141
Chr6 mRNA 3127215 3129926 3127215 ID=MsaT030141.1;Parent=MsaG030141
Chr6 exon 3127215 3127613 3127215 ID=MsaT030141.1.exon4;Parent=MsaT030141.1
Chr6 CDS 3127215 3127613 3127215 ID=cds.MsaT030141.1;Parent=MsaT030141.1
Chr6 exon 3128258 3128497 3128258 ID=MsaT030141.1.exon3;Parent=MsaT030141.1
Chr6 CDS 3128258 3128497 3128258 ID=cds.MsaT030141.1;Parent=MsaT030141.1
Chr6 exon 3129108 3129241 3129108 ID=MsaT030141.1.exon2;Parent=MsaT030141.1
Chr6 CDS 3129108 3129241 3129108 ID=cds.MsaT030141.1;Parent=MsaT030141.1
Chr6 exon 3129833 3129926 3129833 ID=MsaT030141.1.exon1;Parent=MsaT030141.1
Chr6 CDS 3129833 3129926 3129833 ID=cds.MsaT030141.1;Parent=MsaT030141.1
Gene Sequence

>MsaT030141.1

ATGGAGTCTGGTCGTCTTTACTTTGATTCTTCTGCTTGTCGTGGGAATAACATGAACATGCTGTTTCTTGGAAATGCTGATCTTGGTTTTCGAGCAGGAAGGTCAATGATGAGCATGGGGGAAGGCTCAAAAAGGAGACCTTTCTTTAGCTCACCGGATGAACTGTATGATGAGGAGTACTACGAAGAACAGTCACCGGAGAAGAAGCGCCGCCTCACTTCCGAGCAGGTCCATATGTTGGAGAAGAGCTTTGAGGAAGAGAACAAACTTGAGCCAGAGAGGAAAACCCAGTTGGCCAAGAAACTAGGATTGCAACCTAGGCAGGTGGCTGTATGGTTTCAGAACCGTAGGGCTAGATGGAAGACAAAACAACTTGAAAGAGATTATGATGTTCTCAAGTCTTCCTATGATTCCCTACTTTCAACATATGATTCCATTACTAAGGAGAATGAGAAACTCAAATCTGAGGTGGTATCCTTAAATGAGAAGCTTCAAGTTCAAGCTAAAGATATGCTTGAAGAACCTTTGTCAGAAAAGAAAGCTGATCCACTTCCAGTGGACATAGCTCAGATTTTCAGCATCAAGGTGGATGACCACATGAGTTCTGGAAGCGTTGGAAGTGCAGTGGTGGACGAGAGTAGCCCCCGTGTCGTTGGTGTCATTGTTGACAGCGTCGATTCATACTTTCCAGCTGACAACTATGCTGGATGTGTGGCTCCTATTGAAAGAGTTCAATCAGAGGAAGATGATGGGAGTGATGATGGGAGGAACTACTTTGATGTGTTTGTTGCATCTGAAACTGAGCAACATAACCATGAAGAGGGAGAGGCACTGAATTGGTGGGGTAATATGTATTATGTTGCATAA
Protein sequence

>MsaT030141.1

MESGRLYFDSSACRGNNMNMLFLGNADLGFRAGRSMMSMGEGSKRRPFFSSPDELYDEEYYEEQSPEKKRRLTSEQVHMLEKSFEEENKLEPERKTQLAKKLGLQPRQVAVWFQNRRARWKTKQLERDYDVLKSSYDSLLSTYDSITKENEKLKSEVVSLNEKLQVQAKDMLEEPLSEKKADPLPVDIAQIFSIKVDDHMSSGSVGSAVVDESSPRVVGVIVDSVDSYFPADNYAGCVAPIERVQSEEDDGSDDGRNYFDVFVASETEQHNHEEGEALNWWGNMYYVA*