AlfalfaGEDB | MsaG030467

M. sativa ssp. caerulea / MsaG030467

KEGG: K21630
GO: -
PFAM: PF00320.30

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG030467	sp\|O82632\|GATA9_ARATH	45.652	184	44	8	14	197	136	263	2.05e-36	132

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG030467	tr\|G7KQ55\|G7KQ55_MEDTR	92.000	200	4	2	1	200	74	261	9.58e-129	372

TFs/TRs:

Gene ID	Type	Classification
MsaG030467	TF	C2C2-GATA

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG005367	MsaG030467	0.827246	3.103460e-54	8.309039e-52
MsaG007176	MsaG030467	0.800309	2.559420e-48	3.484669e-46
MsaG007848	MsaG030467	0.824059	1.753707e-53	4.299665e-51
MsaG007852	MsaG030467	0.849288	6.757713e-60	3.574741e-57
MsaG008287	MsaG030467	0.826556	4.528240e-54	1.189242e-51
MsaG011294	MsaG030467	0.825754	7.012002e-54	1.800945e-51
MsaG015386	MsaG030467	0.800543	2.294490e-48	3.140615e-46
MsaG022789	MsaG030467	0.807315	9.118102e-50	1.459641e-47
MsaG025778	MsaG030467	0.821234	7.904609e-53	1.796224e-50
MsaG027484	MsaG030467	0.802991	7.256450e-49	1.050097e-46
MsaG028241	MsaG030467	0.803627	5.365457e-49	7.879213e-47
MsaG028652	MsaG030467	0.805588	2.100947e-49	3.228899e-47
MsaG030467	MsaG038035	0.834801	4.423510e-56	1.472904e-53
MsaG030467	MsaG038394	0.810682	1.747659e-50	3.034465e-48
MsaG030467	MsaG045937	0.819704	1.767667e-52	3.857175e-50
MsaG030467	MsaG004914	0.804840	3.007076e-49	4.542104e-47
MsaG030467	MsaG013013	0.819673	1.796258e-52	3.916335e-50
MsaG030467	MsaG035822	0.806447	1.388100e-49	2.177196e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG030467	MtrunA17_Chr6g0461591	92.000	200	4	2	1	200	74	261	1.84e-132	372
MsaG030467	MtrunA17_Chr7g0253101	58.049	205	48	7	1	199	84	256	4.12e-69	211
MsaG030467	MtrunA17_Chr4g0035891	81.538	65	12	0	131	195	211	275	1.01e-34	124
MsaG030467	MtrunA17_Chr1g0199671	80.000	65	13	0	131	195	242	306	3.25e-34	124
MsaG030467	MtrunA17_Chr1g0146231	77.612	67	15	0	131	197	234	300	6.68e-34	123
MsaG030467	MtrunA17_Chr2g0326771	77.143	70	16	0	131	200	216	285	7.30e-34	122
MsaG030467	MtrunA17_Chr8g0372291	73.333	75	18	1	121	195	210	282	9.90e-34	121
MsaG030467	MtrunA17_Chr8g0340401	73.913	69	18	0	129	197	224	292	1.92e-33	122
MsaG030467	MtrunA17_Chr1g0213161	70.270	74	22	0	123	196	144	217	2.21e-33	119
MsaG030467	MtrunA17_Chr3g0140331	68.750	80	20	1	118	197	245	319	8.75e-33	120
MsaG030467	MtrunA17_Chr3g0145121	78.462	65	14	0	131	195	215	279	1.13e-32	119
MsaG030467	MtrunA17_Chr1g0169431	76.471	68	16	0	130	197	171	238	2.17e-32	117
MsaG030467	MtrunA17_Chr5g0404981	80.000	65	13	0	131	195	300	364	2.46e-32	120
MsaG030467	MtrunA17_Chr4g0028961	76.923	65	15	0	131	195	232	296	1.03e-31	117
MsaG030467	MtrunA17_Chr5g0395391	72.059	68	19	0	130	197	269	336	6.35e-31	115
MsaG030467	MtrunA17_Chr5g0395391	70.769	65	19	0	130	194	167	231	9.81e-29	109
MsaG030467	MtrunA17_Chr7g0232671	57.576	66	28	0	127	192	183	248	2.96e-21	88.2
MsaG030467	MtrunA17_Chr7g0232701	56.452	62	27	0	131	192	73	134	1.71e-19	80.5
MsaG030467	MtrunA17_Chr7g0232681	54.839	62	28	0	131	192	132	193	8.82e-19	80.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG030467	AT4G32890.1	45.652	184	44	8	14	197	136	263	2.09e-37	132
MsaG030467	AT3G60530.1	79.710	69	14	0	129	197	156	224	2.19e-36	127
MsaG030467	AT2G45050.1	41.989	181	59	3	17	197	111	245	1.24e-35	126
MsaG030467	AT5G25830.1	38.265	196	90	5	2	197	121	285	6.43e-35	126
MsaG030467	AT3G24050.1	75.000	68	17	0	130	197	193	260	2.07e-34	123
MsaG030467	AT3G54810.1	67.470	83	26	1	113	195	212	293	3.46e-33	121
MsaG030467	AT3G54810.2	67.470	83	26	1	113	195	212	293	3.46e-33	121
MsaG030467	AT5G66320.2	80.000	65	13	0	131	195	249	313	5.08e-33	121
MsaG030467	AT5G66320.1	80.000	65	13	0	131	195	249	313	5.08e-33	121
MsaG030467	AT3G51080.1	81.538	65	12	0	131	195	221	285	5.08e-33	120
MsaG030467	AT4G36240.1	72.464	69	19	0	131	199	164	232	6.25e-33	118
MsaG030467	AT1G08010.2	71.212	66	19	0	130	195	219	284	1.97e-32	119
MsaG030467	AT1G08010.1	71.212	66	19	0	130	195	219	284	1.97e-32	119
MsaG030467	AT1G08010.3	71.212	66	19	0	130	195	219	284	1.97e-32	119
MsaG030467	AT4G34680.1	73.846	65	17	0	131	195	180	244	2.18e-31	115
MsaG030467	AT4G34680.2	73.846	65	17	0	131	195	180	244	2.18e-31	115
MsaG030467	AT1G08000.2	72.727	66	18	0	130	195	217	282	3.24e-31	115
MsaG030467	AT1G08000.1	72.727	66	18	0	130	195	217	282	3.24e-31	115
MsaG030467	AT2G28340.1	66.154	65	22	0	133	197	193	257	1.84e-27	105
MsaG030467	AT3G45170.1	61.538	65	25	0	133	197	117	181	4.70e-26	99.8
MsaG030467	AT5G49300.1	46.296	54	22	1	113	166	25	71	6.14e-11	58.5

Find 34 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTGTTGAAACAAGCTTAT+TGG	0.158008	6:-8347699	MsaT030467.1:CDS
CAGCTCCTAGTACCAAAATT+TGG	0.286985	6:-8347768	MsaT030467.1:CDS
ATGTGAATGTGACCAAATTT+TGG	0.288556	6:+8347756	None:intergenic
CATGCATTGCATAGTGTCTT+TGG	0.309772	6:+8347403	None:intergenic
ATGGTCCTGCCCTCCATTGT+GGG	0.318440	6:+8347431	None:intergenic
TGAGTGCACCTTCTTGCATT+TGG	0.340927	6:+8347472	None:intergenic
ACAATGGAGGGCAGGACCAT+TGG	0.400380	6:-8347428	MsaT030467.1:CDS
AATGGTCCTGCCCTCCATTG+TGG	0.428822	6:+8347430	None:intergenic
GAGAATGCAATCTATTAAGT+AGG	0.465726	6:-8347272	None:intergenic
CAATGGAGGGCAGGACCATT+GGG	0.466961	6:-8347427	MsaT030467.1:CDS
CGGAGCAATGAGTTCGCTGT+CGG	0.489222	6:+8347672	None:intergenic
GTAGGTTGTTGCCAGAGTAT+AGG	0.492955	6:-8347360	MsaT030467.1:CDS
CATAGTGTCTTTGGTCCCAA+TGG	0.499888	6:+8347412	None:intergenic
TGCAAGTAGCTAACAAAAGT+AGG	0.538933	6:+8347325	None:intergenic
CTCTTCGCTGGCCTATACTC+TGG	0.539921	6:+8347349	None:intergenic
AGGACCCCACAATGGAGGGC+AGG	0.548944	6:-8347436	MsaT030467.1:CDS
TGGTCCTGCCCTCCATTGTG+GGG	0.559124	6:+8347432	None:intergenic
AACATATTCCAAATGCAAGA+AGG	0.563153	6:-8347480	MsaT030467.1:CDS
AAGCTTGTTTCAACAAAGGA+GGG	0.570788	6:+8347704	None:intergenic
AGAATGCAATCTATTAAGTA+GGG	0.572327	6:-8347271	None:intergenic
TAAGCTTGTTTCAACAAAGG+AGG	0.573541	6:+8347703	None:intergenic
CATTCACATCTTATAAGTGA+TGG	0.584037	6:-8347742	MsaT030467.1:CDS
TGTGGTGTGAGGTACAAGTC+TGG	0.585109	6:-8347382	MsaT030467.1:CDS
CAATAAGCTTGTTTCAACAA+AGG	0.588114	6:+8347700	None:intergenic
ACAAAGGACCCCACAATGGA+GGG	0.595325	6:-8347440	MsaT030467.1:CDS
GTGTGAGGTACAAGTCTGGT+AGG	0.597780	6:-8347378	MsaT030467.1:CDS
GCACTCATTGTTTGTCACAA+AGG	0.603068	6:-8347456	MsaT030467.1:CDS
CACAAAGGACCCCACAATGG+AGG	0.605327	6:-8347441	MsaT030467.1:CDS
ACAAAAGTAGGACTCTTCGC+TGG	0.624466	6:+8347337	None:intergenic
AAAGTCTCTGCAGTTGCGTG+CGG	0.637759	6:-8347637	MsaT030467.1:CDS
TGATGTTAATGATGATGATG+AGG	0.646926	6:-8347503	MsaT030467.1:CDS
TGTCACAAAGGACCCCACAA+TGG	0.681006	6:-8347444	MsaT030467.1:CDS
TATGCAATGCATGTGGTGTG+AGG	0.734829	6:-8347393	MsaT030467.1:CDS
AAGACACTATGCAATGCATG+TGG	0.764250	6:-8347400	MsaT030467.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	8347273	8347875	8347273	ID=MsaG030467
Chr6	mRNA	8347273	8347875	8347273	ID=MsaT030467.1;Parent=MsaG030467
Chr6	exon	8347273	8347875	8347273	ID=MsaT030467.1.exon1;Parent=MsaT030467.1
Chr6	CDS	8347273	8347875	8347273	ID=cds.MsaT030467.1;Parent=MsaT030467.1

Gene Sequence

>MsaT030467.1

ATGCAAAAACCTCAACAACAAAATCAATCTTATTTGCAAAATTTTGTTGTTCCTGGAAAAGCAAGAAGCAAGAGAAAAAGACTTTCAGCTCCTAGTACCAAAATTTGGTCACATTCACATCTTATAAGTGATGGTAATTTGATTTCTGACCCTCCTTTGTTGAAACAAGCTTATTGGTTAGCCGACAGCGAACTCATTGCTCCGAAAAATGAGCAAAAAGTCTCTGCAGTTGCGTGCGGAGATCAGACAGAAGCAAAAAGAAGGGTGAAGAGAGTGGTAAAAATGGAGAAAGAGAAAGAGAGTTTTGAGGTTGGAATTATTCAAGTTAAGAATAGTGAGAATAATAATGATGATGTTAATGATGATGATGAGGAACATATTCCAAATGCAAGAAGGTGCACTCATTGTTTGTCACAAAGGACCCCACAATGGAGGGCAGGACCATTGGGACCAAAGACACTATGCAATGCATGTGGTGTGAGGTACAAGTCTGGTAGGTTGTTGCCAGAGTATAGGCCAGCGAAGAGTCCTACTTTTGTTAGCTACTTGCATTCAAATTCACACAAGAAAGTTCTTGAGATGAGAATGCAATCTATTAAGTAG

Protein sequence

>MsaT030467.1

MQKPQQQNQSYLQNFVVPGKARSKRKRLSAPSTKIWSHSHLISDGNLISDPPLLKQAYWLADSELIAPKNEQKVSAVACGDQTEAKRRVKRVVKMEKEKESFEVGIIQVKNSENNNDDVNDDDEEHIPNARRCTHCLSQRTPQWRAGPLGPKTLCNACGVRYKSGRLLPEYRPAKSPTFVSYLHSNSHKKVLEMRMQSIK*