AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG030526


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030526 MtrunA17_Chr6g0460951 98.678 227 3 0 1 227 1 227 4.35e-168 462
MsaG030526 MtrunA17_Chr1g0184271 46.277 188 88 4 40 220 12 193 7.14e-49 160
MsaG030526 MtrunA17_Chr7g0260971 67.961 103 33 0 39 141 13 115 3.88e-47 155
MsaG030526 MtrunA17_Chr7g0261031 64.815 108 38 0 39 146 7 114 4.47e-47 155
MsaG030526 MtrunA17_Chr8g0351701 66.667 102 34 0 40 141 23 124 3.27e-46 153
MsaG030526 MtrunA17_Chr5g0435641 54.701 117 53 0 41 157 8 124 3.81e-45 149
MsaG030526 MtrunA17_Chr1g0184281 64.356 101 36 0 40 140 15 115 1.07e-44 148
MsaG030526 MtrunA17_Chr7g0245871 62.745 102 38 0 40 141 27 128 2.50e-44 148
MsaG030526 MtrunA17_Chr8g0351721 60.504 119 46 1 40 157 18 136 2.83e-44 147
MsaG030526 MtrunA17_Chr6g0460941 68.182 88 28 0 40 127 7 94 8.39e-42 137
MsaG030526 MtrunA17_Chr7g0245861 67.778 90 27 2 40 127 12 101 6.73e-36 124
MsaG030526 MtrunA17_Chr4g0058651 52.294 109 51 1 39 147 10 117 4.03e-35 123
MsaG030526 MtrunA17_Chr2g0311601 47.706 109 56 1 38 146 11 118 6.79e-35 122
MsaG030526 MtrunA17_Chr4g0043421 49.074 108 54 1 39 146 14 120 7.05e-35 122
MsaG030526 MtrunA17_Chr3g0114061 43.382 136 72 2 39 170 7 141 8.51e-35 121
MsaG030526 MtrunA17_Chr3g0129071 51.376 109 52 1 38 146 45 152 2.22e-34 122
MsaG030526 MtrunA17_Chr3g0106101 50.000 104 51 1 39 142 6 108 5.26e-34 120
MsaG030526 MtrunA17_Chr6g0449641 48.276 116 57 2 39 154 8 120 5.53e-34 119
MsaG030526 MtrunA17_Chr3g0117121 51.818 110 50 2 39 148 9 115 7.43e-34 120
MsaG030526 MtrunA17_Chr7g0227511 51.923 104 49 1 39 142 10 112 8.63e-34 119
MsaG030526 MtrunA17_Chr5g0438091 49.107 112 56 1 39 150 38 148 1.31e-33 119
MsaG030526 MtrunA17_Chr5g0437401 50.495 101 49 1 41 141 12 111 1.66e-33 119
MsaG030526 MtrunA17_Chr5g0437571 49.038 104 52 1 39 142 5 107 1.68e-32 115
MsaG030526 MtrunA17_Chr5g0402611 52.427 103 48 1 39 141 7 108 3.41e-32 119
MsaG030526 MtrunA17_Chr4g0030001 42.963 135 68 4 37 164 21 153 2.11e-31 114
MsaG030526 MtrunA17_Chr3g0115431 50.485 103 50 1 39 141 8 109 3.18e-31 114
MsaG030526 MtrunA17_Chr6g0449651 45.690 116 60 2 39 154 66 178 3.77e-31 113
MsaG030526 MtrunA17_Chr3g0113891 47.917 96 49 1 41 136 15 109 9.23e-31 112
MsaG030526 MtrunA17_Chr3g0097221 45.299 117 60 2 39 155 6 118 8.13e-30 112
MsaG030526 MtrunA17_Chr5g0432451 52.778 108 50 1 38 145 41 147 1.07e-29 110
MsaG030526 MtrunA17_Chr1g0200201 44.348 115 61 2 41 154 73 185 1.39e-28 107
MsaG030526 MtrunA17_Chr5g0444351 45.283 106 57 1 41 146 6 110 2.61e-28 104
MsaG030526 MtrunA17_Chr8g0353981 56.098 82 35 1 39 120 6 86 5.65e-27 103
MsaG030526 MtrunA17_Chr7g0225111 40.000 110 65 1 37 146 2 110 3.73e-26 98.2
MsaG030526 MtrunA17_Chr8g0374601 44.762 105 57 1 37 141 23 126 5.83e-26 102
MsaG030526 MtrunA17_Chr8g0374591 39.815 108 64 1 34 141 2 108 4.15e-24 93.2
MsaG030526 MtrunA17_Chr8g0374581 40.367 109 63 2 35 142 3 110 1.11e-20 86.7
MsaG030526 MtrunA17_Chr4g0059901 41.584 101 58 1 41 141 11 110 1.54e-20 87.0
MsaG030526 MtrunA17_Chr2g0330201 36.634 101 63 1 41 141 20 119 2.37e-19 84.7
MsaG030526 MtrunA17_Chr8g0342351 37.624 101 62 1 41 141 13 112 7.82e-19 82.4
MsaG030526 MtrunA17_Chr4g0040831 36.190 105 63 2 41 142 16 119 1.77e-17 77.8
MsaG030526 MtrunA17_Chr6g0460931 49.524 105 33 3 140 227 6 107 3.62e-17 74.3
MsaG030526 MtrunA17_Chr6g0452671 34.653 101 65 1 41 141 12 111 4.13e-17 76.6
MsaG030526 MtrunA17_Chr6g0452691 38.235 102 61 2 41 141 10 110 5.51e-17 75.9
MsaG030526 MtrunA17_Chr6g0452661 33.028 109 68 3 37 141 3 110 6.98e-16 74.3
MsaG030526 MtrunA17_Chr4g0055441 41.975 81 45 2 41 120 3 82 9.42e-15 69.3
MsaG030526 MtrunA17_Chr5g0439111 30.488 164 98 6 37 190 3 160 1.50e-13 67.4
MsaG030526 MtrunA17_Chr2g0326041 52.000 50 23 1 37 86 30 78 1.93e-13 63.9
MsaG030526 MtrunA17_Chr2g0326051 35.294 102 56 2 86 181 5 102 7.14e-12 60.8
MsaG030526 MtrunA17_Chr4g0046201 27.072 181 99 5 39 191 5 180 1.27e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030526 AT3G03760.1 67.785 149 43 1 39 187 50 193 3.29e-62 196
MsaG030526 AT2G42430.1 48.889 180 84 2 39 218 14 185 3.49e-49 161
MsaG030526 AT3G58190.1 61.864 118 44 1 40 157 11 127 3.78e-48 158
MsaG030526 AT2G42440.1 48.571 140 60 1 39 178 6 133 6.16e-44 148
MsaG030526 AT2G45420.1 65.385 104 36 0 43 146 40 143 1.20e-43 147
MsaG030526 AT5G06080.1 53.226 124 56 1 39 162 6 127 1.27e-43 145
MsaG030526 AT4G00220.1 62.500 104 39 0 43 146 20 123 3.43e-43 145
MsaG030526 AT4G00210.1 63.725 102 37 0 40 141 11 112 1.09e-42 144
MsaG030526 AT2G31310.1 56.604 106 46 0 39 144 6 111 1.14e-42 143
MsaG030526 AT2G45410.1 50.000 148 58 4 43 189 19 151 3.71e-40 136
MsaG030526 AT3G27650.1 52.885 104 48 1 39 142 38 140 1.08e-34 121
MsaG030526 AT5G63090.4 42.675 157 80 4 39 190 10 161 4.83e-34 120
MsaG030526 AT5G63090.3 42.675 157 80 4 39 190 10 161 4.83e-34 120
MsaG030526 AT5G63090.2 42.675 157 80 4 39 190 10 161 4.83e-34 120
MsaG030526 AT5G63090.1 42.675 157 80 4 39 190 10 161 4.83e-34 120
MsaG030526 AT2G30340.1 47.863 117 58 2 38 154 50 163 2.05e-33 121
MsaG030526 AT2G30340.2 47.863 117 58 2 38 154 51 164 2.05e-33 121
MsaG030526 AT2G30130.1 46.774 124 63 2 36 159 4 124 3.11e-33 119
MsaG030526 AT1G07900.1 45.378 119 62 2 37 154 30 146 3.45e-33 119
MsaG030526 AT3G11090.1 48.039 102 52 1 41 142 12 112 2.78e-32 115
MsaG030526 AT2G28500.1 47.748 111 55 2 37 146 52 160 5.86e-32 117
MsaG030526 AT1G16530.1 46.789 109 56 2 38 145 12 119 8.86e-32 114
MsaG030526 AT5G66870.1 41.290 155 79 3 39 193 6 148 1.73e-31 117
MsaG030526 AT1G31320.1 48.148 108 55 1 39 146 12 118 1.97e-30 111
MsaG030526 AT2G23660.2 46.154 104 55 1 39 142 4 106 4.63e-29 111
MsaG030526 AT2G23660.3 46.154 104 55 1 39 142 4 106 4.63e-29 111
MsaG030526 AT2G23660.1 46.154 104 55 1 39 142 4 106 4.63e-29 111
MsaG030526 AT2G40470.2 51.282 117 56 1 38 154 18 133 6.02e-29 108
MsaG030526 AT2G40470.1 51.282 117 56 1 38 154 43 158 6.10e-29 108
MsaG030526 AT1G65620.1 53.398 103 47 1 39 141 8 109 7.21e-29 107
MsaG030526 AT1G65620.4 53.398 103 47 1 39 141 8 109 7.21e-29 107
MsaG030526 AT1G65620.3 53.398 103 47 1 39 141 8 109 7.21e-29 107
MsaG030526 AT1G65620.5 53.398 103 47 1 39 141 8 109 7.21e-29 107
MsaG030526 AT1G65620.2 53.398 103 47 1 39 141 8 109 7.21e-29 107
MsaG030526 AT3G26660.1 44.444 108 52 2 41 141 6 112 2.16e-28 104
MsaG030526 AT3G26620.1 44.444 108 52 2 41 141 6 112 2.94e-28 103
MsaG030526 AT3G50510.1 38.532 109 66 1 33 141 5 112 5.78e-24 95.1
MsaG030526 AT3G50510.2 38.532 109 66 1 33 141 5 112 5.78e-24 95.1
MsaG030526 AT1G72980.1 41.818 110 62 2 39 147 12 120 6.75e-21 87.4
MsaG030526 AT3G13850.1 30.409 171 102 4 41 210 37 191 2.28e-19 84.3
MsaG030526 AT5G35900.1 33.981 103 67 1 39 141 4 105 1.01e-16 76.3
MsaG030526 AT3G47870.1 32.673 101 67 1 41 141 37 136 7.51e-15 72.8
MsaG030526 AT1G06280.1 41.333 75 43 1 41 115 25 98 3.05e-14 69.3
MsaG030526 AT4G22700.1 31.579 95 62 2 41 134 6 98 2.28e-12 63.9

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTGAAACGGCGGCTGATA+TGG 0.243600 6:-9454965 None:intergenic
AACAACTTCAACCCTGGTTT+TGG 0.268597 6:+9455073 MsaT030526.1:CDS
AGGCTAGCTGATCCAGTTTA+TGG 0.286193 6:+9454543 MsaT030526.1:CDS
CCTTCTTGCTTTGCAGCAAC+AGG 0.312094 6:+9454578 MsaT030526.1:CDS
AAATTGTTGCAGCAGCTTCC+TGG 0.332792 6:-9454503 None:intergenic
GACTGTGATGGCTGAGTCAC+AGG 0.379048 6:+9454215 None:intergenic
GGTGGTGATGGTAGTAGTTC+GGG 0.392136 6:+9454237 MsaT030526.1:CDS
GTGGACACACAGCCATAAAC+TGG 0.395590 6:-9454555 None:intergenic
GAAGCACTTGTCCAACATTC+CGG 0.397219 6:+9454470 MsaT030526.1:CDS
TGCATCTTTGCACCCTACTT+TGG 0.399115 6:+9454384 MsaT030526.1:CDS
TGAAGTTGTTCATGTTCATA+AGG 0.399289 6:-9455060 None:intergenic
TAGCCTAGCTTGAGCCTCAT+AGG 0.409914 6:-9454526 None:intergenic
ATGGTAGTAGTTCGGGAAAC+AGG 0.414773 6:+9454244 MsaT030526.1:CDS
TGCACAGGTACATTGAAGTT+TGG 0.417807 6:-9454998 None:intergenic
GTTGAAACGGCGGCTGATAT+GGG 0.423151 6:-9454964 None:intergenic
TGCAAACTCAATTGATGAAT+AGG 0.424003 6:+9454906 MsaT030526.1:CDS
GCGGCTGTGCACAAGGTGTT+TGG 0.424834 6:+9454432 MsaT030526.1:CDS
GGGTGGTGATGGTAGTAGTT+CGG 0.433902 6:+9454236 MsaT030526.1:CDS
GCACCACACGGCGTTATAAC+CGG 0.444947 6:-9454327 None:intergenic
ATATCAGCCGCCGTTTCAAC+TGG 0.453062 6:+9454967 MsaT030526.1:CDS
TTGGAATATGCTGGCTGCAC+AGG 0.466534 6:-9455013 None:intergenic
TTGCAGCAGCTTCCTGGCGA+TGG 0.481070 6:-9454497 None:intergenic
GGCTCTAAGCTCTGTGATGA+AGG 0.484149 6:-9455115 None:intergenic
TTTCTCAAAACGAGACATCC+TGG 0.484547 6:-9455149 None:intergenic
GTTGTTCATGTTCATAAGGT+TGG 0.485083 6:-9455056 None:intergenic
GCTTCCTGGCGATGGCTAGC+CGG 0.485119 6:-9454489 None:intergenic
TTGAGGAGGAAGTGTAGTAG+TGG 0.488789 6:+9454360 MsaT030526.1:CDS
CCAAGGTGCGGCTAAGTTTG+CGG 0.493536 6:+9454413 MsaT030526.1:CDS
AAGAGCAACACAAGACGAAC+CGG 0.499358 6:+9454308 MsaT030526.1:CDS
GGTTGCTGTACCAGTTGAAA+CGG 0.507203 6:-9454977 None:intergenic
GGTGGAAGCAGATGAGTTGT+TGG 0.513070 6:-9455032 None:intergenic
TCCATTGTGAGGCCAAAACC+AGG 0.515508 6:-9455085 None:intergenic
CCTGTTGCTGCAAAGCAAGA+AGG 0.518305 6:-9454578 None:intergenic
GCGATGGCTAGCCGGAATGT+TGG 0.521327 6:-9454481 None:intergenic
AACATGAACAACTTCAACCC+TGG 0.523993 6:+9455067 MsaT030526.1:CDS
TGCTGCAACAATTTCCTATG+AGG 0.529239 6:+9454512 MsaT030526.1:CDS
GTTCATGTTCATAAGGTTGG+TGG 0.542354 6:-9455053 None:intergenic
CTGTTGCTGCAAAGCAAGAA+GGG 0.544374 6:-9454577 None:intergenic
ACCTTGGTCAGTACCAAAGT+AGG 0.553693 6:-9454397 None:intergenic
GCTGAGTCACAGGGTGGTGA+TGG 0.557362 6:+9454225 MsaT030526.1:CDS
CATTCCGGCTAGCCATCGCC+AGG 0.568999 6:+9454485 MsaT030526.1:CDS
AAACTCAATTGATGAATAGG+AGG 0.570071 6:+9454909 MsaT030526.1:CDS
TAAGTTTGCGGCTGTGCACA+AGG 0.570935 6:+9454425 MsaT030526.1:CDS
CCGCAAACTTAGCCGCACCT+TGG 0.577994 6:-9454413 None:intergenic
CATGTTCATAAGGTTGGTGG+TGG 0.580499 6:-9455050 None:intergenic
TTTCCTATGAGGCTCAAGCT+AGG 0.591556 6:+9454523 MsaT030526.1:CDS
CCTTGGTCAGTACCAAAGTA+GGG 0.599955 6:-9454396 None:intergenic
GATGAGTTGTTGGAATATGC+TGG 0.605347 6:-9455022 None:intergenic
TTGAAACGGCGGCTGATATG+GGG 0.606891 6:-9454963 None:intergenic
CCCTACTTTGGTACTGACCA+AGG 0.613176 6:+9454396 MsaT030526.1:CDS
GGTGGCAGCTATGCAAGCAG+AGG 0.634590 6:+9454874 MsaT030526.1:intron
GTGGTGCTTGCAAGTTCTTG+AGG 0.646814 6:+9454343 MsaT030526.1:CDS
CTTTGGTACTGACCAAGGTG+CGG 0.655594 6:+9454401 MsaT030526.1:CDS
TTGCTGCAAAGCAAGAAGGG+TGG 0.671870 6:-9454574 None:intergenic
AAGGTGCCGTCTCCATTGTG+AGG 0.672207 6:-9455096 None:intergenic
ACTGTGATGGCTGAGTCACA+GGG 0.673977 6:+9454216 None:intergenic
GTGCTTGCAAGTTCTTGAGG+AGG 0.680170 6:+9454346 MsaT030526.1:CDS
TGCTGTACCAGTTGAAACGG+CGG 0.689555 6:-9454974 None:intergenic
AAGAACTTGCAAGCACCACA+CGG 0.689772 6:-9454339 None:intergenic
CCATTGTGAGGCCAAAACCA+GGG 0.697828 6:-9455084 None:intergenic
GAACCGGTTATAACGCCGTG+TGG 0.738703 6:+9454324 MsaT030526.1:CDS
GTGATGGCTGAGTCACAGGG+TGG 0.776633 6:+9454219 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 9454222 9455180 9454222 ID=MsaG030526
Chr6 mRNA 9454222 9455180 9454222 ID=MsaT030526.1;Parent=MsaG030526
Chr6 exon 9454222 9454599 9454222 ID=MsaT030526.1.exon1;Parent=MsaT030526.1
Chr6 CDS 9454222 9454599 9454222 ID=cds.MsaT030526.1;Parent=MsaT030526.1
Chr6 exon 9454875 9455180 9454875 ID=MsaT030526.1.exon2;Parent=MsaT030526.1
Chr6 CDS 9454875 9455180 9454875 ID=cds.MsaT030526.1;Parent=MsaT030526.1
Gene Sequence

>MsaT030526.1

ATGGCTGAGTCACAGGGTGGTGATGGTAGTAGTTCGGGAAACAGGCGCAATAAAAGCGCAAATGCTGCTGCAAAGCGTGCAATAGCAAGAGCAACACAAGACGAACCGGTTATAACGCCGTGTGGTGCTTGCAAGTTCTTGAGGAGGAAGTGTAGTAGTGGATGCATCTTTGCACCCTACTTTGGTACTGACCAAGGTGCGGCTAAGTTTGCGGCTGTGCACAAGGTGTTTGGTGCTAGCAATGTGTCGAAGCACTTGTCCAACATTCCGGCTAGCCATCGCCAGGAAGCTGCTGCAACAATTTCCTATGAGGCTCAAGCTAGGCTAGCTGATCCAGTTTATGGCTGTGTGTCCACCCTTCTTGCTTTGCAGCAACAGGTGGCAGCTATGCAAGCAGAGGTCTCGATGATGCAAACTCAATTGATGAATAGGAGGCTTGCATATGCAAATGCAAGTACTCTTCAAGCCCCATATCAGCCGCCGTTTCAACTGGTACAGCAACCAAACTTCAATGTACCTGTGCAGCCAGCATATTCCAACAACTCATCTGCTTCCACCACCAACCTTATGAACATGAACAACTTCAACCCTGGTTTTGGCCTCACAATGGAGACGGCACCTTCATCACAGAGCTTAGAGCCTTTTCAAAACTCCAGGATGTCTCGTTTTGAGAAAGATGATTAA

Protein sequence

>MsaT030526.1

MAESQGGDGSSSGNRRNKSANAAAKRAIARATQDEPVITPCGACKFLRRKCSSGCIFAPYFGTDQGAAKFAAVHKVFGASNVSKHLSNIPASHRQEAAATISYEAQARLADPVYGCVSTLLALQQQVAAMQAEVSMMQTQLMNRRLAYANASTLQAPYQPPFQLVQQPNFNVPVQPAYSNNSSASTTNLMNMNNFNPGFGLTMETAPSSQSLEPFQNSRMSRFEKDD*