AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG030557

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030557 MtrunA17_Chr6g0460721 89.941 338 25 2 1 338 1 329 0.0 614
MsaG030557 MtrunA17_Chr6g0460731 76.540 341 66 2 1 327 1 341 0.0 520
MsaG030557 MtrunA17_Chr7g0254161 49.422 346 137 9 10 337 8 333 1.66e-96 292
MsaG030557 MtrunA17_Chr7g0217581 46.524 187 78 5 48 231 191 358 2.40e-44 160
MsaG030557 MtrunA17_Chr4g0042481 48.045 179 72 5 51 228 85 243 3.39e-42 152
MsaG030557 MtrunA17_Chr6g0453321 39.344 244 119 6 47 270 96 330 4.72e-42 152
MsaG030557 MtrunA17_Chr3g0092111 38.462 273 128 10 48 319 105 338 5.88e-41 149
MsaG030557 MtrunA17_Chr8g0355261 32.877 292 129 8 10 268 11 268 2.08e-35 133
MsaG030557 MtrunA17_Chr5g0417571 51.351 111 45 2 47 151 99 206 5.01e-31 119
MsaG030557 MtrunA17_Chr2g0283811 57.895 95 32 3 55 142 7 100 3.18e-29 110
MsaG030557 MtrunA17_Chr5g0417551 45.802 131 64 3 45 173 88 213 3.16e-27 110
MsaG030557 MtrunA17_Chr5g0417601 50.476 105 49 1 46 150 100 201 3.24e-27 110
MsaG030557 MtrunA17_Chr8g0346551 63.492 63 23 0 84 146 19 81 8.66e-26 103
MsaG030557 MtrunA17_Chr5g0417561 52.041 98 42 2 45 142 80 172 9.81e-26 105
MsaG030557 MtrunA17_Chr4g0047001 49.474 95 37 2 61 144 16 110 2.66e-25 105
MsaG030557 MtrunA17_Chr3g0127201 59.155 71 29 0 73 143 11 81 2.66e-25 103
MsaG030557 MtrunA17_Chr8g0374251 50.000 88 44 0 89 176 68 155 3.46e-25 103
MsaG030557 MtrunA17_Chr8g0367161 57.534 73 25 1 72 144 21 87 4.61e-25 103
MsaG030557 MtrunA17_Chr8g0374111 50.000 86 43 0 89 174 29 114 7.14e-25 100
MsaG030557 MtrunA17_Chr2g0326001 55.844 77 33 1 68 143 18 94 9.87e-25 101
MsaG030557 MtrunA17_Chr4g0010151 62.903 62 23 0 82 143 75 136 1.16e-24 102
MsaG030557 MtrunA17_Chr4g0030051 57.895 76 31 1 67 141 18 93 2.29e-24 100
MsaG030557 MtrunA17_Chr4g0031411 48.387 93 43 1 82 174 68 155 2.72e-24 101
MsaG030557 MtrunA17_Chr2g0328021 47.475 99 51 1 76 174 49 146 3.01e-24 100
MsaG030557 MtrunA17_Chr2g0308011 75.510 49 12 0 89 137 37 85 4.80e-24 97.8
MsaG030557 MtrunA17_Chr2g0282051 70.690 58 16 1 87 144 44 100 1.77e-23 97.8
MsaG030557 MtrunA17_Chr1g0188851 50.000 80 35 1 67 141 16 95 2.53e-23 97.8
MsaG030557 MtrunA17_Chr4g0047761 52.747 91 27 2 69 144 37 126 3.35e-23 98.2
MsaG030557 MtrunA17_Chr2g0282441 59.375 64 26 0 77 140 13 76 6.08e-23 97.4
MsaG030557 MtrunA17_Chr4g0061761 67.925 53 17 0 87 139 38 90 7.22e-23 97.1
MsaG030557 MtrunA17_Chr7g0237921 55.556 72 30 1 74 143 59 130 7.85e-23 97.4
MsaG030557 MtrunA17_Chr8g0341671 69.231 52 16 0 89 140 20 71 1.36e-22 94.4
MsaG030557 MtrunA17_Chr7g0223311 70.909 55 15 1 90 143 78 132 5.47e-22 95.5
MsaG030557 MtrunA17_Chr5g0411951 63.636 55 20 0 85 139 39 93 7.81e-22 94.4
MsaG030557 MtrunA17_Chr3g0126201 56.164 73 30 1 73 143 33 105 8.78e-22 94.4
MsaG030557 MtrunA17_Chr2g0282511 71.429 49 14 0 89 137 45 93 1.54e-21 93.2
MsaG030557 MtrunA17_Chr1g0177251 66.038 53 18 0 91 143 14 66 4.71e-21 91.7
MsaG030557 MtrunA17_Chr3g0117601 58.065 62 25 1 79 139 50 111 7.59e-21 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030557 AT3G47500.1 47.368 152 62 3 45 196 71 204 4.96e-38 141
MsaG030557 AT5G39660.3 57.391 115 36 2 55 156 92 206 1.33e-37 140
MsaG030557 AT5G39660.1 57.391 115 36 2 55 156 92 206 1.33e-37 140
MsaG030557 AT5G39660.2 57.391 115 36 2 55 156 92 206 1.33e-37 140
MsaG030557 AT1G26790.1 44.382 178 78 4 9 165 10 187 3.26e-35 132
MsaG030557 AT5G62430.1 63.043 92 29 2 68 154 30 121 2.11e-34 128
MsaG030557 AT2G34140.1 46.154 130 44 2 13 142 8 111 1.04e-32 120
MsaG030557 AT1G69570.1 52.632 114 52 1 77 188 120 233 1.70e-32 125
MsaG030557 AT1G29160.1 76.667 60 14 0 83 142 56 115 9.81e-31 115
MsaG030557 AT3G55370.1 57.143 70 30 0 85 154 72 141 3.62e-25 103
MsaG030557 AT3G55370.2 57.143 70 30 0 85 154 72 141 4.22e-25 104
MsaG030557 AT3G55370.3 57.143 70 30 0 85 154 117 186 8.53e-25 103
MsaG030557 AT2G28810.2 41.414 99 55 1 45 140 30 128 2.58e-24 101
MsaG030557 AT2G28810.1 41.414 99 55 1 45 140 47 145 3.12e-24 101
MsaG030557 AT5G60200.1 37.086 151 80 2 83 218 49 199 3.90e-24 99.8
MsaG030557 AT1G51700.1 55.714 70 29 1 71 140 17 84 5.74e-24 97.8
MsaG030557 AT3G21270.1 71.698 53 15 0 89 141 29 81 5.80e-24 97.8
MsaG030557 AT5G60850.1 70.909 55 16 0 89 143 51 105 7.68e-24 100
MsaG030557 AT4G24060.1 60.938 64 25 0 80 143 44 107 9.71e-24 100
MsaG030557 AT3G61850.2 63.333 60 22 0 84 143 57 116 1.34e-23 99.0
MsaG030557 AT3G61850.3 63.333 60 22 0 84 143 57 116 1.34e-23 99.0
MsaG030557 AT2G37590.1 62.712 59 22 0 85 143 85 143 1.68e-23 99.4
MsaG030557 AT3G61850.1 63.333 60 22 0 84 143 69 128 1.75e-23 98.6
MsaG030557 AT1G28310.1 67.857 56 18 0 89 144 27 82 1.85e-23 99.0
MsaG030557 AT1G28310.2 67.857 56 18 0 89 144 51 106 2.53e-23 99.0
MsaG030557 AT3G61850.5 63.333 60 22 0 84 143 57 116 2.61e-23 98.6
MsaG030557 AT3G61850.4 63.333 60 22 0 84 143 69 128 3.16e-23 98.6
MsaG030557 AT3G45610.1 58.065 62 26 0 85 146 36 97 4.06e-23 96.7
MsaG030557 AT5G65590.1 69.091 55 17 0 89 143 41 95 4.67e-23 97.8
MsaG030557 AT3G50410.1 67.273 55 18 0 89 143 30 84 8.91e-23 95.9
MsaG030557 AT1G07640.1 67.857 56 17 1 89 143 21 76 9.23e-23 96.3
MsaG030557 AT5G66940.1 41.322 121 58 3 76 195 26 134 1.28e-22 94.7
MsaG030557 AT1G07640.2 67.857 56 17 1 89 143 77 132 1.45e-22 97.1
MsaG030557 AT4G00940.1 54.167 72 29 1 77 144 52 123 1.69e-22 95.9
MsaG030557 AT4G00940.3 54.167 72 29 1 77 144 52 123 1.69e-22 95.9
MsaG030557 AT4G00940.2 54.167 72 29 1 77 144 52 123 1.69e-22 95.9
MsaG030557 AT1G07640.3 67.857 56 17 1 89 143 85 140 2.03e-22 96.7
MsaG030557 AT5G02460.1 59.677 62 25 0 82 143 88 149 2.11e-22 97.4
MsaG030557 AT1G64620.1 58.333 60 25 0 78 137 38 97 4.15e-22 95.9
MsaG030557 AT2G28510.1 67.857 56 18 0 91 146 50 105 4.75e-22 94.7
MsaG030557 AT4G38000.1 60.656 61 23 1 88 147 40 100 1.36e-21 92.4
MsaG030557 AT4G21050.1 50.000 78 37 1 69 144 2 79 2.20e-21 90.9
MsaG030557 AT3G52440.1 46.512 86 46 0 91 176 27 112 4.47e-21 91.3
MsaG030557 AT3G52440.2 46.512 86 46 0 91 176 46 131 6.62e-21 90.9
MsaG030557 AT2G46590.1 62.264 53 20 0 84 136 63 115 1.70e-20 91.3
MsaG030557 AT2G46590.2 62.264 53 20 0 84 136 75 127 1.79e-20 91.7
MsaG030557 AT1G47655.1 65.306 49 17 0 89 137 29 77 2.08e-20 88.2
MsaG030557 AT1G21340.1 68.000 50 16 0 91 140 40 89 2.17e-20 89.4
MsaG030557 AT5G62940.1 57.812 64 26 1 77 139 60 123 2.35e-20 91.3
MsaG030557 AT4G21040.1 56.140 57 25 0 91 147 27 83 1.05e-16 78.6
MsaG030557 AT4G21080.1 54.386 57 26 0 91 147 27 83 2.75e-16 77.8

Find 57 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTTCTCTTGTGGTTTCT+AGG 0.197262 6:-9985632 None:intergenic
AATGGCATTGTCCTAAAATT+TGG 0.232669 6:+9985563 MsaT030557.1:CDS
AATTTGTTCCTGAATCATTT+AGG 0.243494 6:+9985951 MsaT030557.1:CDS
GTTAATAATTGTAGAGAAAA+TGG 0.253304 6:+9985665 MsaT030557.1:CDS
GTCGGAAGTGTCATAGATAT+TGG 0.311203 6:+9985366 MsaT030557.1:CDS
TTCAGGTGCTCAAATAATAA+TGG 0.321797 6:+9985545 MsaT030557.1:CDS
ACATCGAATCGTTAGATTCA+TGG 0.328881 6:-9985604 None:intergenic
GATCCATTCCTAAATGATTC+AGG 0.331263 6:-9985959 None:intergenic
AAGCACTGTGGAAGATAATA+TGG 0.348391 6:+9985211 MsaT030557.1:CDS
TTGTTCAAGCTTTGATTCAA+TGG 0.349711 6:-9985815 None:intergenic
AGGAATGGATCAGTTTCAAT+AGG 0.385406 6:+9985971 MsaT030557.1:CDS
GCTCTTTGGAAAGAAGATTC+AGG 0.390549 6:+9984014 MsaT030557.1:CDS
TATCTGAATCCGAACATAAT+AGG 0.400322 6:+9985750 MsaT030557.1:CDS
ATGCAAATGTGTAACCCTTA+TGG 0.424721 6:+9985863 MsaT030557.1:CDS
CCTGAAAACCACAGCAGATT+CGG 0.430545 6:-9985528 None:intergenic
GTCTGAAGATGTTCGGCATA+TGG 0.438201 6:-9985913 None:intergenic
AGAAACGTTCCTTTCGGTGC+AGG 0.439748 6:+9985407 MsaT030557.1:CDS
GACGTTACTTTCAGAATTTG+AGG 0.441263 6:-9985498 None:intergenic
CTTGAACAATGTTCCTTCCA+TGG 0.451475 6:+9985829 MsaT030557.1:CDS
CGCCACATGATTGTAGCTTC+CGG 0.453024 6:+9985464 MsaT030557.1:CDS
GAACTAGGATCAACACCATA+AGG 0.457100 6:-9985878 None:intergenic
AGAGCTACCCTGCATCTTCA+TGG 0.465844 6:+9985786 MsaT030557.1:CDS
GGACATTGAACAATCTTGTC+CGG 0.468204 6:-9985272 None:intergenic
TTCCTTTCGGTGCAGGGAGA+CGG 0.475764 6:+9985414 MsaT030557.1:CDS
ACTTACCATGGTTGAAGAGT+TGG 0.478391 6:-9984037 None:intergenic
ATGTTCGGTCTGAAGATGTT+CGG 0.485708 6:-9985920 None:intergenic
CATTGTCCTAAAATTTGGTC+CGG 0.488780 6:+9985568 MsaT030557.1:CDS
TGTGTATCTTCTGTGACAAA+TGG 0.500907 6:+9985707 MsaT030557.1:CDS
GGAATCATCCATGAAGATGC+AGG 0.501713 6:-9985794 None:intergenic
AACGTTTCTCATTGTTCCAC+CGG 0.503306 6:-9985393 None:intergenic
ACAATGAGAAACGTTCCTTT+CGG 0.504607 6:+9985401 MsaT030557.1:CDS
TTGCATCAATATTTCTCTTG+TGG 0.508819 6:-9985640 None:intergenic
ACAAATTGCAAGCCAATGTT+CGG 0.513658 6:-9985935 None:intergenic
GTTCCTGAATCATTTAGGAA+TGG 0.513930 6:+9985956 MsaT030557.1:CDS
TGGCACCACTGCATTGAACT+AGG 0.522209 6:-9985893 None:intergenic
GTGTTGAAACTGTTGCATCT+TGG 0.528822 6:-9985293 None:intergenic
GAAACGTTCCTTTCGGTGCA+GGG 0.532355 6:+9985408 MsaT030557.1:CDS
TGTCATAGATATTGGACTGC+CGG 0.542196 6:+9985374 MsaT030557.1:CDS
AACTAGGATCAACACCATAA+GGG 0.543659 6:-9985877 None:intergenic
CTCTTCTACCGAATCTGCTG+TGG 0.544865 6:+9985520 MsaT030557.1:CDS
TTCCGTCTCCCTGCACCGAA+AGG 0.555844 6:-9985416 None:intergenic
ATTGAAGATTGAACCATGGA+AGG 0.561855 6:-9985842 None:intergenic
TGTCACAGAAGATACACATA+AGG 0.564721 6:-9985702 None:intergenic
AACATCTTCAGACCGAACAT+TGG 0.582195 6:+9985923 MsaT030557.1:CDS
TAGCTAGTAGTAGATGAAGA+AGG 0.592040 6:-9986013 None:intergenic
ATTTGAGGTTGAAATTCCAC+CGG 0.595342 6:-9985483 None:intergenic
TCAGGCCAACTCTTCAACCA+TGG 0.613442 6:+9984032 MsaT030557.1:CDS
GTGACAAATGGTTATACTCG+AGG 0.618192 6:+9985719 MsaT030557.1:CDS
CATAGATATTGGACTGCCGG+TGG 0.627706 6:+9985377 MsaT030557.1:CDS
GAATCATCCATGAAGATGCA+GGG 0.636010 6:-9985793 None:intergenic
TTGATCCTAGTTCAATGCAG+TGG 0.640980 6:+9985888 MsaT030557.1:CDS
TTGCATTGAAGATTGAACCA+TGG 0.644235 6:-9985846 None:intergenic
CACATGATTGTAGCTTCCGG+TGG 0.649268 6:+9985467 MsaT030557.1:CDS
TGCAGTAACTCAAAGCACAA+AGG 0.653363 6:+9985110 MsaT030557.1:CDS
ACTACAATGTCAGTCAACCT+CGG 0.657312 6:+9985336 MsaT030557.1:CDS
CACCGGAAGCTACAATCATG+TGG 0.658948 6:-9985466 None:intergenic
AAAAGTTTACACAAGCACTG+TGG 0.736976 6:+9985199 MsaT030557.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 9983970 9986036 9983970 ID=MsaG030557
Chr6 mRNA 9983970 9986036 9983970 ID=MsaT030557.1;Parent=MsaG030557
Chr6 exon 9983970 9984053 9983970 ID=MsaT030557.1.exon1;Parent=MsaT030557.1
Chr6 CDS 9983970 9984053 9983970 ID=cds.MsaT030557.1;Parent=MsaT030557.1
Chr6 exon 9985104 9986036 9985104 ID=MsaT030557.1.exon2;Parent=MsaT030557.1
Chr6 CDS 9985104 9986036 9985104 ID=cds.MsaT030557.1;Parent=MsaT030557.1
Gene Sequence

>MsaT030557.1

ATGAATGGTGAAGGTGACATGTGTGTTAAAGATTTTAGTATCATGCTCTTTGGAAAGAAGATTCAGGCCAACTCTTCAACCATGAGTTCTTGCAGTAACTCAAAGCACAAAGGAAATGAAATGCCTCATATTCAAAACATTGAGAAATCAAAGAATTCTTCTAATGAAGAAAATGAATCAAAAGTTTACACAAGCACTGTGGAAGATAATATGGAAACTAGCAGCACAGATCAAGACAAAGTCCTCAAAAAACCGGACAAGATTGTTCAATGTCCAAGATGCAACAGTTTCAACACGAAATTTTGTTATTTCAATAACTACAATGTCAGTCAACCTCGGCATTTTTGTCGGAAGTGTCATAGATATTGGACTGCCGGTGGAACAATGAGAAACGTTCCTTTCGGTGCAGGGAGACGGAAGAACAAGCAGATAGCTTCTCAATATCGCCACATGATTGTAGCTTCCGGTGGAATTTCAACCTCAAATTCTGAAAGTAACGTCTCTTCTACCGAATCTGCTGTGGTTTTCAGGTGCTCAAATAATAATGGCATTGTCCTAAAATTTGGTCCGGAAAATGCATTTCTCCATGAATCTAACGATTCGATGTTTAATCCTAGAAACCACAAGAGAAATATTGATGCAAGTGTTAATAATTGTAGAGAAAATGGAGATGAAGAATCATCCTTATGTGTATCTTCTGTGACAAATGGTTATACTCGAGGAAACGAGTTATCTGAATCCGAACATAATAGGTCAAAACTATTGCAGAGCTACCCTGCATCTTCATGGATGATTCCATTGAATCAAAGCTTGAACAATGTTCCTTCCATGGTTCAATCTTCAATGCAAATGTGTAACCCTTATGGTGTTGATCCTAGTTCAATGCAGTGGTGCCATATGCCGAACATCTTCAGACCGAACATTGGCTTGCAATTTGTTCCTGAATCATTTAGGAATGGATCAGTTTCAATAGGTTCAAATAATTGTTTTTCGCCTTCTTCATCTACTACTAGCTAG
Protein sequence

>MsaT030557.1

MNGEGDMCVKDFSIMLFGKKIQANSSTMSSCSNSKHKGNEMPHIQNIEKSKNSSNEENESKVYTSTVEDNMETSSTDQDKVLKKPDKIVQCPRCNSFNTKFCYFNNYNVSQPRHFCRKCHRYWTAGGTMRNVPFGAGRRKNKQIASQYRHMIVASGGISTSNSESNVSSTESAVVFRCSNNNGIVLKFGPENAFLHESNDSMFNPRNHKRNIDASVNNCRENGDEESSLCVSSVTNGYTRGNELSESEHNRSKLLQSYPASSWMIPLNQSLNNVPSMVQSSMQMCNPYGVDPSSMQWCHMPNIFRPNIGLQFVPESFRNGSVSIGSNNCFSPSSSTTS*