AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG030730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030730 MtrunA17_Chr1g0172971 79.487 117 23 1 1 116 1 117 7.68e-59 193
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCATACTCAACAAGAAT+TGG 0.300636 6:-12758909 None:intergenic
TTTAAGTCCGAAAATCAAAA+AGG 0.371320 6:-12759200 None:intergenic
CCATGATTCTTTATCACTAA+AGG 0.406174 6:-12758670 None:intergenic
TAAAGAATCATGGAAGTCTT+TGG 0.408500 6:+12758680 MsaT030730.1:CDS
ATAACACTGGATTCTCTGTT+AGG 0.417808 6:+12758751 MsaT030730.1:CDS
CGAGAAGTAAATGAAGTTCA+AGG 0.430014 6:+12759086 MsaT030730.1:CDS
AGCTGGAATATATTTGAAGT+TGG 0.433136 6:+12758968 MsaT030730.1:CDS
TGAATGTCTATAAGATATAT+AGG 0.445219 6:+12759000 MsaT030730.1:CDS
GAAAGGGTTGGTTGTAAAGC+TGG 0.447509 6:+12758951 MsaT030730.1:CDS
CGTTATGCTTGTAATAAAGA+AGG 0.449439 6:+12758834 MsaT030730.1:CDS
GCTTTAATAAATTCTAAAGC+TGG 0.450264 6:+12759116 MsaT030730.1:CDS
CTCAAGTAGCGCAAATGCTC+TGG 0.460440 6:-12759056 None:intergenic
ACAAAATAAGAAATCGGATA+AGG 0.469178 6:+12758800 MsaT030730.1:CDS
AATAAAGAAGGTTTCAAGAA+GGG 0.472567 6:+12758846 MsaT030730.1:CDS
TTACAACCAACCCTTTCCTC+TGG 0.485330 6:-12758945 None:intergenic
TTTAATTATGCATATAACAC+TGG 0.485542 6:+12758738 MsaT030730.1:CDS
CAATTCACAAAATAAGAAAT+CGG 0.490263 6:+12758794 MsaT030730.1:CDS
TGTCTATAAGATATATAGGT+GGG 0.523740 6:+12759004 MsaT030730.1:CDS
CCTTTAGTGATAAAGAATCA+TGG 0.543522 6:+12758670 MsaT030730.1:CDS
GAGAACATCTTATGAAGTGA+TGG 0.551307 6:+12759148 MsaT030730.1:CDS
AAATGCTCTGGTTTATGTAA+TGG 0.552634 6:-12759044 None:intergenic
TGAAAATTCAGTTATTGATG+CGG 0.555963 6:+12758629 MsaT030730.1:CDS
TAATAAAGAAGGTTTCAAGA+AGG 0.561053 6:+12758845 MsaT030730.1:CDS
AGAATTGGATTGTCACATGT+TGG 0.564120 6:-12758894 None:intergenic
GGGTGAAGGTGTTGGAGGCA+CGG 0.565649 6:+12759169 MsaT030730.1:CDS
GAAGTGATGGGTGAAGGTGT+TGG 0.603704 6:+12759161 MsaT030730.1:CDS
ATATCAACTTCATCTGAGAG+TGG 0.614980 6:+12758594 MsaT030730.1:CDS
GTGATGGGTGAAGGTGTTGG+AGG 0.621481 6:+12759164 MsaT030730.1:CDS
AGAACATCTTATGAAGTGAT+GGG 0.643044 6:+12759149 MsaT030730.1:CDS
ATGTCTATAAGATATATAGG+TGG 0.643489 6:+12759003 MsaT030730.1:CDS
TCTTATGAAGTGATGGGTGA+AGG 0.656961 6:+12759155 MsaT030730.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 12758585 12759208 12758585 ID=MsaG030730
Chr6 mRNA 12758585 12759208 12758585 ID=MsaT030730.1;Parent=MsaG030730
Chr6 exon 12758585 12759208 12758585 ID=MsaT030730.1.exon1;Parent=MsaT030730.1
Chr6 CDS 12758585 12759208 12758585 ID=cds.MsaT030730.1;Parent=MsaT030730.1
Gene Sequence

>MsaT030730.1

ATGGATTTGATATCAACTTCATCTGAGAGTGGACAATTGACGATTGAAAATTCAGTTATTGATGCGGATAGTTCTCAATGTTACTCCTTTAGTGATAAAGAATCATGGAAGTCTTTGGTCTTTAAATCTATAAAAGAGATTGAAGATTTTTATTTTAATTATGCATATAACACTGGATTCTCTGTTAGGAGTATGTTGAAGTCCAAGAACAATTCACAAAATAAGAAATCGGATAAGGTACATTATGTGCGTTATGCTTGTAATAAAGAAGGTTTCAAGAAGGGAAGTCTGCTTAATCCTAAAAATAGGCCAACATGTGACAATCCAATTCTTGTTGAGTATGAGAAAGCTAAAGAAAAACCAGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGTTGGATGAAGCGTTGAATGTCTATAAGATATATAGGTGGGAAGTTGATCACTGTCATCCATTACATAAACCAGAGCATTTGCGCTACTTGAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTTGCTTTAATAAATTCTAAAGCTGGAATGTCGATGAGAACATCTTATGAAGTGATGGGTGAAGGTGTTGGAGGCACGGAGAATCTTCCTTTTTGA
Protein sequence

>MsaT030730.1

MDLISTSSESGQLTIENSVIDADSSQCYSFSDKESWKSLVFKSIKEIEDFYFNYAYNTGFSVRSMLKSKNNSQNKKSDKVHYVRYACNKEGFKKGSLLNPKNRPTCDNPILVEYEKAKEKPEERVGCKAGIYLKLDEALNVYKIYRWEVDHCHPLHKPEHLRYLRSFREVNEVQGQLALINSKAGMSMRTSYEVMGEGVGGTENLPF*