Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG030766 | tr|A0A396HGW4|A0A396HGW4_MEDTR | 57.407 | 54 | 23 | 0 | 90 | 143 | 224 | 277 | 4.90e-14 | 79.0 |
MsaG030766 | tr|A0A396HGW4|A0A396HGW4_MEDTR | 88.811 | 143 | 16 | 0 | 1 | 143 | 1 | 143 | 4.17e-83 | 268 |
Gene ID | Type | Classification |
---|---|---|
MsaG030766 | TF | FAR1 |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000113 | MsaG030766 | 0.809117 | 3.782437e-50 | 6.323208e-48 |
MsaG001323 | MsaG030766 | 0.810985 | 1.504284e-50 | 2.631313e-48 |
MsaG003311 | MsaG030766 | 0.800412 | 2.439914e-48 | 3.329787e-46 |
MsaG003684 | MsaG030766 | 0.806876 | 1.128143e-49 | 1.787320e-47 |
MsaG003859 | MsaG030766 | 0.827165 | 3.244828e-54 | 8.667841e-52 |
MsaG003972 | MsaG030766 | 0.803231 | 6.475218e-49 | 9.422734e-47 |
MsaG004432 | MsaG030766 | 0.804120 | 4.243239e-49 | 6.303104e-47 |
MsaG004746 | MsaG030766 | 0.806174 | 1.583802e-49 | 2.468050e-47 |
MsaG005004 | MsaG030766 | 0.822622 | 3.783449e-53 | 8.922163e-51 |
MsaG005321 | MsaG030766 | 0.809627 | 2.943406e-50 | 4.981403e-48 |
MsaG006013 | MsaG030766 | 0.811224 | 1.335687e-50 | 2.350186e-48 |
MsaG006327 | MsaG030766 | 0.800804 | 2.030893e-48 | 2.796247e-46 |
MsaG006633 | MsaG030766 | 0.802956 | 7.375838e-49 | 1.066513e-46 |
MsaG006668 | MsaG030766 | 0.801136 | 1.738889e-48 | 2.412132e-46 |
MsaG007117 | MsaG030766 | 0.806839 | 1.148394e-49 | 1.817801e-47 |
MsaG007990 | MsaG030766 | 0.801327 | 1.589880e-48 | 2.215011e-46 |
MsaG007988 | MsaG030766 | 0.809262 | 3.521353e-50 | 5.907078e-48 |
MsaG008288 | MsaG030766 | 0.809313 | 3.434006e-50 | 5.767557e-48 |
MsaG008299 | MsaG030766 | 0.817062 | 6.964703e-52 | 1.419046e-49 |
MsaG010628 | MsaG030766 | 0.804254 | 3.980187e-49 | 5.930792e-47 |
MsaG011881 | MsaG030766 | 0.809269 | 3.509214e-50 | 5.887733e-48 |
MsaG012411 | MsaG030766 | 0.833996 | 7.029129e-56 | 2.284753e-53 |
MsaG013228 | MsaG030766 | 0.811338 | 1.262280e-50 | 2.227221e-48 |
MsaG013578 | MsaG030766 | 0.800864 | 1.974714e-48 | 2.722526e-46 |
MsaG013846 | MsaG030766 | 0.842364 | 5.032523e-58 | 2.117383e-55 |
MsaG016002 | MsaG030766 | 0.814730 | 2.295415e-51 | 4.406984e-49 |
MsaG016796 | MsaG030766 | 0.812648 | 6.560558e-51 | 1.195565e-48 |
MsaG017087 | MsaG030766 | 0.818703 | 2.979363e-52 | 6.332818e-50 |
MsaG017192 | MsaG030766 | 0.800623 | 2.210820e-48 | 3.031590e-46 |
MsaG017985 | MsaG030766 | 0.810614 | 1.808016e-50 | 3.134121e-48 |
MsaG019134 | MsaG030766 | 0.800807 | 2.027951e-48 | 2.792394e-46 |
MsaG019335 | MsaG030766 | 0.808838 | 4.336340e-50 | 7.200754e-48 |
MsaG019540 | MsaG030766 | 0.832535 | 1.618145e-55 | 5.039177e-53 |
MsaG019589 | MsaG030766 | 0.800147 | 2.760772e-48 | 3.745045e-46 |
MsaG020648 | MsaG030766 | 0.816049 | 1.171698e-51 | 2.326000e-49 |
MsaG020712 | MsaG030766 | 0.809892 | 2.582447e-50 | 4.398667e-48 |
MsaG021510 | MsaG030766 | 0.839851 | 2.284734e-57 | 8.877826e-55 |
MsaG021565 | MsaG030766 | 0.807547 | 8.143015e-50 | 1.310827e-47 |
MsaG021836 | MsaG030766 | 0.812958 | 5.614128e-51 | 1.031047e-48 |
MsaG022095 | MsaG030766 | 0.800014 | 2.936234e-48 | 3.971395e-46 |
MsaG022320 | MsaG030766 | 0.806479 | 1.366819e-49 | 2.145437e-47 |
MsaG022936 | MsaG030766 | 0.800129 | 2.783058e-48 | 3.773804e-46 |
MsaG023350 | MsaG030766 | 0.808239 | 5.812009e-50 | 9.512961e-48 |
MsaG023967 | MsaG030766 | 0.816105 | 1.138459e-51 | 2.263237e-49 |
MsaG026867 | MsaG030766 | 0.803703 | 5.174330e-49 | 7.612311e-47 |
MsaG028254 | MsaG030766 | 0.826830 | 3.897442e-54 | 1.031434e-51 |
MsaG028590 | MsaG030766 | 0.804898 | 2.924516e-49 | 4.423331e-47 |
MsaG028760 | MsaG030766 | 0.811134 | 1.396881e-50 | 2.452370e-48 |
MsaG028831 | MsaG030766 | 0.804634 | 3.318393e-49 | 4.988279e-47 |
MsaG028883 | MsaG030766 | 0.800910 | 1.933222e-48 | 2.668014e-46 |
MsaG029083 | MsaG030766 | 0.804948 | 2.856433e-49 | 4.325247e-47 |
MsaG029193 | MsaG030766 | 0.809566 | 3.032182e-50 | 5.124350e-48 |
MsaG029311 | MsaG030766 | 0.810764 | 1.678384e-50 | 2.920074e-48 |
MsaG029370 | MsaG030766 | 0.801551 | 1.430807e-48 | 2.003547e-46 |
MsaG029652 | MsaG030766 | 0.825594 | 7.647649e-54 | 1.955459e-51 |
MsaG029835 | MsaG030766 | 0.809218 | 3.599504e-50 | 6.031721e-48 |
MsaG029894 | MsaG030766 | 0.814746 | 2.276121e-51 | 4.371867e-49 |
MsaG030766 | MsaG031410 | 0.808126 | 6.143843e-50 | 1.002853e-47 |
MsaG030766 | MsaG032765 | 0.816540 | 9.108616e-52 | 1.831005e-49 |
MsaG030766 | MsaG033569 | 0.804706 | 3.207317e-49 | 4.829285e-47 |
MsaG030766 | MsaG035457 | 0.804532 | 3.484154e-49 | 5.225224e-47 |
MsaG030766 | MsaG041930 | 0.812787 | 6.118807e-51 | 1.118943e-48 |
MsaG030766 | MsaG043710 | 0.805081 | 2.679871e-49 | 4.070480e-47 |
MsaG030766 | MsaG044369 | 0.837375 | 9.890626e-57 | 3.558656e-54 |
MsaG030766 | MsaG045037 | 0.812185 | 8.271507e-51 | 1.490144e-48 |
MsaG030766 | MsaG045517 | 0.808212 | 5.890511e-50 | 9.635037e-48 |
MsaG030766 | MsaG045618 | 0.829024 | 1.162562e-54 | 3.271959e-52 |
MsaG030766 | MsaG046057 | 0.801575 | 1.414716e-48 | 1.982086e-46 |
MsaG030766 | MsaG046459 | 0.824005 | 1.805013e-53 | 4.419076e-51 |
MsaG030766 | MsaG047185 | 0.809529 | 3.088776e-50 | 5.215260e-48 |
MsaG030766 | MsaG002462 | 0.835118 | 3.682603e-56 | 1.237895e-53 |
MsaG030766 | MsaG002669 | 0.819135 | 2.379166e-52 | 5.114861e-50 |
MsaG030766 | MsaG008743 | 0.803140 | 6.759945e-49 | 9.816301e-47 |
MsaG030766 | MsaG013545 | 0.819225 | 2.269938e-52 | 4.891534e-50 |
MsaG030766 | MsaG013938 | 0.808267 | 5.734540e-50 | 9.392417e-48 |
MsaG030766 | MsaG012992 | 0.825531 | 7.912671e-54 | 2.019835e-51 |
MsaG030766 | MsaG018951 | 0.811540 | 1.141023e-50 | 2.023286e-48 |
MsaG030766 | MsaG032827 | 0.808509 | 5.094399e-50 | 8.392577e-48 |
MsaG030766 | MsaG030125 | 0.802712 | 8.278993e-49 | 1.190389e-46 |
MsaG030766 | MsaG037402 | 0.813172 | 5.044066e-51 | 9.313076e-49 |
MsaG030766 | MsaG037397 | 0.808946 | 4.112919e-50 | 6.847465e-48 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG030766 | MtrunA17_Chr6g0458391 | 88.811 | 143 | 16 | 0 | 1 | 143 | 1 | 143 | 8.03e-87 | 268 |
MsaG030766 | MtrunA17_Chr6g0458391 | 57.407 | 54 | 23 | 0 | 90 | 143 | 224 | 277 | 9.43e-18 | 79.0 |
MsaG030766 | MtrunA17_Chr1g0203721 | 56.863 | 51 | 22 | 0 | 93 | 143 | 65 | 115 | 1.47e-15 | 72.8 |
MsaG030766 | MtrunA17_Chr3g0082121 | 50.725 | 69 | 32 | 1 | 78 | 144 | 28 | 96 | 2.34e-13 | 66.2 |
MsaG030766 | MtrunA17_Chr5g0401121 | 36.145 | 83 | 50 | 1 | 65 | 144 | 27 | 109 | 3.76e-12 | 60.5 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Find 37 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
TCGAATTCCATGCCAATATT+TGG | 0.165608 | 6:-13535506 | None:intergenic |
AATACGAAATTGGTCTTCAT+TGG | 0.304948 | 6:-13535387 | None:intergenic |
GTCTATGGCAGGTGAATCTT+CGG | 0.322499 | 6:-13535637 | None:intergenic |
ATATATGTGGCGCCAAATAT+TGG | 0.340354 | 6:+13535494 | MsaT030766.1:CDS |
ATCAACAAAAGTAGGATAGA+TGG | 0.360653 | 6:+13535602 | MsaT030766.1:CDS |
TGCTAGCGTCAATACGAAAT+TGG | 0.360891 | 6:-13535397 | None:intergenic |
GTCTTCATTGGAAGTATTTG+AGG | 0.393019 | 6:-13535375 | None:intergenic |
TAGGACGAAAACCTTGTCTA+TGG | 0.403464 | 6:-13535652 | None:intergenic |
CACTTTACTGTCTGCAGCAC+AGG | 0.414311 | 6:-13535267 | None:intergenic |
GAAAGGTTTCAGCATAAGAA+AGG | 0.423022 | 6:+13535574 | MsaT030766.1:CDS |
GTGACACGAAACTTGCCATT+TGG | 0.430039 | 6:-13535755 | None:intergenic |
TCTGCAGCACAGGAATCCTT+CGG | 0.440191 | 6:-13535257 | None:intergenic |
TCCATCTGAGTAAGGCCAAC+AGG | 0.444164 | 6:-13535332 | None:intergenic |
CTGCAGCACAGGAATCCTTC+GGG | 0.455610 | 6:-13535256 | None:intergenic |
TCTATGGCAGGTGAATCTTC+GGG | 0.473803 | 6:-13535636 | None:intergenic |
AGGATTTCATCAACAAAAGT+AGG | 0.483308 | 6:+13535594 | MsaT030766.1:CDS |
ACTATTTCTCGTCAGCCAAA+TGG | 0.488160 | 6:+13535740 | MsaT030766.1:intron |
AACAAGTATGCTATTATGAA+AGG | 0.494730 | 6:+13535557 | MsaT030766.1:CDS |
CTCTGCTATAGAGGTAGCAT+CGG | 0.505769 | 6:-13535441 | None:intergenic |
TCCTGTTGGCCTTACTCAGA+TGG | 0.506262 | 6:+13535331 | MsaT030766.1:CDS |
CAATGATTTCCATCTGAGTA+AGG | 0.506463 | 6:-13535340 | None:intergenic |
TATTGGCATGGAATTCGAAT+CGG | 0.511843 | 6:+13535511 | MsaT030766.1:CDS |
AGTAACGGTCTCTGCTATAG+AGG | 0.516564 | 6:-13535450 | None:intergenic |
ACGAAAACCTTGTCTATGGC+AGG | 0.521032 | 6:-13535648 | None:intergenic |
TCTGCTATAGAGGTAGCATC+GGG | 0.521378 | 6:-13535440 | None:intergenic |
CTCATTTGTTACATCAGTAA+CGG | 0.549751 | 6:-13535465 | None:intergenic |
AGTAGTGATTGATATGGGGC+AGG | 0.561170 | 6:+13535223 | None:intergenic |
AGTGCAAATGCTCATCCTGT+TGG | 0.564210 | 6:+13535317 | MsaT030766.1:CDS |
TGTTGTAACACTTGTAAGCA+TGG | 0.566414 | 6:-13535538 | None:intergenic |
AAATGAGGAGGAAATATATG+TGG | 0.601435 | 6:+13535481 | MsaT030766.1:CDS |
TACTGATGTAACAAATGAGG+AGG | 0.606710 | 6:+13535469 | MsaT030766.1:CDS |
CGTTACTGATGTAACAAATG+AGG | 0.607025 | 6:+13535466 | MsaT030766.1:CDS |
AGATTCACCTGCCATAGACA+AGG | 0.613560 | 6:+13535641 | MsaT030766.1:CDS |
TTTCGTATTGACGCTAGCAG+AGG | 0.623277 | 6:+13535401 | MsaT030766.1:CDS |
TGTGGCGCCAAATATTGGCA+TGG | 0.636247 | 6:+13535499 | MsaT030766.1:CDS |
GCAGGAGAATCAATGCCCGA+AGG | 0.685839 | 6:+13535241 | MsaT030766.1:CDS |
CTGCTATAGAGGTAGCATCG+GGG | 0.739070 | 6:-13535439 | None:intergenic |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr6 | gene | 13535236 | 13535780 | 13535236 | ID=MsaG030766 |
Chr6 | mRNA | 13535236 | 13535780 | 13535236 | ID=MsaT030766.1;Parent=MsaG030766 |
Chr6 | exon | 13535236 | 13535662 | 13535236 | ID=MsaT030766.1.exon1;Parent=MsaT030766.1 |
Chr6 | CDS | 13535236 | 13535662 | 13535236 | ID=cds.MsaT030766.1;Parent=MsaT030766.1 |
Chr6 | exon | 13535755 | 13535780 | 13535755 | ID=MsaT030766.1.exon2;Parent=MsaT030766.1 |
Chr6 | CDS | 13535755 | 13535780 | 13535755 | ID=cds.MsaT030766.1;Parent=MsaT030766.1 |
Gene Sequence |
Protein sequence |