AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG030766


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030766 MtrunA17_Chr6g0458391 88.811 143 16 0 1 143 1 143 8.03e-87 268
MsaG030766 MtrunA17_Chr6g0458391 57.407 54 23 0 90 143 224 277 9.43e-18 79.0
MsaG030766 MtrunA17_Chr1g0203721 56.863 51 22 0 93 143 65 115 1.47e-15 72.8
MsaG030766 MtrunA17_Chr3g0082121 50.725 69 32 1 78 144 28 96 2.34e-13 66.2
MsaG030766 MtrunA17_Chr5g0401121 36.145 83 50 1 65 144 27 109 3.76e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGAATTCCATGCCAATATT+TGG 0.165608 6:-13535506 None:intergenic
AATACGAAATTGGTCTTCAT+TGG 0.304948 6:-13535387 None:intergenic
GTCTATGGCAGGTGAATCTT+CGG 0.322499 6:-13535637 None:intergenic
ATATATGTGGCGCCAAATAT+TGG 0.340354 6:+13535494 MsaT030766.1:CDS
ATCAACAAAAGTAGGATAGA+TGG 0.360653 6:+13535602 MsaT030766.1:CDS
TGCTAGCGTCAATACGAAAT+TGG 0.360891 6:-13535397 None:intergenic
GTCTTCATTGGAAGTATTTG+AGG 0.393019 6:-13535375 None:intergenic
TAGGACGAAAACCTTGTCTA+TGG 0.403464 6:-13535652 None:intergenic
CACTTTACTGTCTGCAGCAC+AGG 0.414311 6:-13535267 None:intergenic
GAAAGGTTTCAGCATAAGAA+AGG 0.423022 6:+13535574 MsaT030766.1:CDS
GTGACACGAAACTTGCCATT+TGG 0.430039 6:-13535755 None:intergenic
TCTGCAGCACAGGAATCCTT+CGG 0.440191 6:-13535257 None:intergenic
TCCATCTGAGTAAGGCCAAC+AGG 0.444164 6:-13535332 None:intergenic
CTGCAGCACAGGAATCCTTC+GGG 0.455610 6:-13535256 None:intergenic
TCTATGGCAGGTGAATCTTC+GGG 0.473803 6:-13535636 None:intergenic
AGGATTTCATCAACAAAAGT+AGG 0.483308 6:+13535594 MsaT030766.1:CDS
ACTATTTCTCGTCAGCCAAA+TGG 0.488160 6:+13535740 MsaT030766.1:intron
AACAAGTATGCTATTATGAA+AGG 0.494730 6:+13535557 MsaT030766.1:CDS
CTCTGCTATAGAGGTAGCAT+CGG 0.505769 6:-13535441 None:intergenic
TCCTGTTGGCCTTACTCAGA+TGG 0.506262 6:+13535331 MsaT030766.1:CDS
CAATGATTTCCATCTGAGTA+AGG 0.506463 6:-13535340 None:intergenic
TATTGGCATGGAATTCGAAT+CGG 0.511843 6:+13535511 MsaT030766.1:CDS
AGTAACGGTCTCTGCTATAG+AGG 0.516564 6:-13535450 None:intergenic
ACGAAAACCTTGTCTATGGC+AGG 0.521032 6:-13535648 None:intergenic
TCTGCTATAGAGGTAGCATC+GGG 0.521378 6:-13535440 None:intergenic
CTCATTTGTTACATCAGTAA+CGG 0.549751 6:-13535465 None:intergenic
AGTAGTGATTGATATGGGGC+AGG 0.561170 6:+13535223 None:intergenic
AGTGCAAATGCTCATCCTGT+TGG 0.564210 6:+13535317 MsaT030766.1:CDS
TGTTGTAACACTTGTAAGCA+TGG 0.566414 6:-13535538 None:intergenic
AAATGAGGAGGAAATATATG+TGG 0.601435 6:+13535481 MsaT030766.1:CDS
TACTGATGTAACAAATGAGG+AGG 0.606710 6:+13535469 MsaT030766.1:CDS
CGTTACTGATGTAACAAATG+AGG 0.607025 6:+13535466 MsaT030766.1:CDS
AGATTCACCTGCCATAGACA+AGG 0.613560 6:+13535641 MsaT030766.1:CDS
TTTCGTATTGACGCTAGCAG+AGG 0.623277 6:+13535401 MsaT030766.1:CDS
TGTGGCGCCAAATATTGGCA+TGG 0.636247 6:+13535499 MsaT030766.1:CDS
GCAGGAGAATCAATGCCCGA+AGG 0.685839 6:+13535241 MsaT030766.1:CDS
CTGCTATAGAGGTAGCATCG+GGG 0.739070 6:-13535439 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 13535236 13535780 13535236 ID=MsaG030766
Chr6 mRNA 13535236 13535780 13535236 ID=MsaT030766.1;Parent=MsaG030766
Chr6 exon 13535236 13535662 13535236 ID=MsaT030766.1.exon1;Parent=MsaT030766.1
Chr6 CDS 13535236 13535662 13535236 ID=cds.MsaT030766.1;Parent=MsaT030766.1
Chr6 exon 13535755 13535780 13535755 ID=MsaT030766.1.exon2;Parent=MsaT030766.1
Chr6 CDS 13535755 13535780 13535755 ID=cds.MsaT030766.1;Parent=MsaT030766.1
Gene Sequence

>MsaT030766.1

ATGGGGCAGGAGAATCAATGCCCGAAGGATTCCTGTGCTGCAGACAGTAAAGTGAACGTTAGCAATGAGACTGATTGTACAAGTGCAAATGCTCATCCTGTTGGCCTTACTCAGATGGAAATCATTGATGAATGTCAGACCTCAAATACTTCCAATGAAGACCAATTTCGTATTGACGCTAGCAGAGGACCGATGTGTCAAAACCCCGATGCTACCTCTATAGCAGAGACCGTTACTGATGTAACAAATGAGGAGGAAATATATGTGGCGCCAAATATTGGCATGGAATTCGAATCGGAAGACCATGCTTACAAGTGTTACAACAAGTATGCTATTATGAAAGGTTTCAGCATAAGAAAGGATTTCATCAACAAAAGTAGGATAGATGGTTCAGTGATGTCCCGAAGATTCACCTGCCATAGACAAGCCAAATGGCAAGTTTCGTGTCACTAG

Protein sequence

>MsaT030766.1

MGQENQCPKDSCAADSKVNVSNETDCTSANAHPVGLTQMEIIDECQTSNTSNEDQFRIDASRGPMCQNPDATSIAETVTDVTNEEEIYVAPNIGMEFESEDHAYKCYNKYAIMKGFSIRKDFINKSRIDGSVMSRRFTCHRQAKWQVSCH*