AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG030986


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030986 MtrunA17_Chr6g0456061 99.500 200 1 0 1 200 1 200 4.59e-147 406
MsaG030986 MtrunA17_Chr7g0220571 64.286 196 58 6 1 189 1 191 5.75e-66 201
MsaG030986 MtrunA17_Chr7g0225661 59.627 161 56 5 9 167 8 161 1.47e-54 172
MsaG030986 MtrunA17_Chr8g0341311 59.375 160 48 5 9 167 8 151 3.62e-54 171
MsaG030986 MtrunA17_Chr4g0035414 52.326 86 38 2 73 158 16 98 1.85e-18 78.6
MsaG030986 MtrunA17_Chr1g0155551 40.196 102 61 0 64 165 4 105 1.47e-17 76.3
MsaG030986 MtrunA17_Chr3g0144941 50.000 80 40 0 79 158 19 98 3.61e-17 75.1
MsaG030986 MtrunA17_Chr4g0054291 36.620 142 66 3 43 162 8 147 1.09e-16 74.3
MsaG030986 MtrunA17_Chr5g0400901 41.509 106 57 1 65 165 31 136 5.50e-13 63.9
MsaG030986 MtrunA17_Chr1g0194681 39.362 94 52 2 65 158 7 95 5.01e-12 61.2
MsaG030986 MtrunA17_Chr8g0378901 34.375 96 63 0 63 158 8 103 7.37e-12 60.8
MsaG030986 MtrunA17_Chr5g0410531 48.438 64 33 0 90 153 310 373 5.49e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG030986 AT3G30530.1 74.545 110 25 2 65 171 53 162 5.14e-50 160
MsaG030986 AT5G38800.1 61.207 116 38 2 71 186 53 161 1.23e-41 138
MsaG030986 AT5G15830.1 60.909 110 43 0 57 166 41 150 6.01e-39 132
MsaG030986 AT1G13600.1 56.693 127 45 3 71 195 68 186 1.46e-36 126
MsaG030986 AT2G04038.1 64.583 96 34 0 70 165 54 149 4.20e-36 124
MsaG030986 AT1G68880.1 46.316 95 49 1 74 168 33 125 1.57e-21 86.3
MsaG030986 AT1G75390.1 47.423 97 50 1 62 158 14 109 8.84e-19 80.1
MsaG030986 AT5G60830.1 45.192 104 52 2 89 189 70 171 1.80e-17 77.4
MsaG030986 AT1G75390.2 46.237 93 49 1 62 154 14 105 3.63e-16 72.0
MsaG030986 AT2G18160.1 48.611 72 37 0 87 158 28 99 1.91e-15 71.2
MsaG030986 AT3G62420.1 44.304 79 44 0 80 158 15 93 4.80e-15 69.7
MsaG030986 AT4G34590.1 45.946 74 40 0 85 158 22 95 3.41e-13 65.1
MsaG030986 AT2G22850.1 36.522 115 61 3 70 172 106 220 3.57e-11 60.8
MsaG030986 AT2G22850.2 36.522 115 61 3 70 172 106 220 3.57e-11 60.8

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTTGGTCACAAGTTCTT+TGG 0.273917 6:-17761786 MsaT030986.1:CDS
TTAGGTAATTACCATATAAT+TGG 0.292562 6:+17761998 None:intergenic
GGACTAAAAGAAGGGATTAA+AGG 0.295805 6:+17761622 None:intergenic
TCAAGTTCAATGGATCTTCT+TGG 0.335013 6:-17761553 MsaT030986.1:CDS
AAATTCTCAAACCAATTATA+TGG 0.356492 6:-17762009 MsaT030986.1:CDS
ATTGTTGATGAGGGGTGTTT+AGG 0.356793 6:+17761980 None:intergenic
TCCTCTCATTGATGAGACTA+AGG 0.387624 6:+17761881 None:intergenic
AGAAACACCTTGATGAGCTT+TGG 0.415939 6:-17761801 MsaT030986.1:CDS
TCAGCTTAAAGAAGAAGCTT+TGG 0.440839 6:-17761683 MsaT030986.1:CDS
TGTTACTTCTCTTGTTTGCA+TGG 0.445242 6:+17762131 None:intergenic
CTGCTTATGCATGATGATTG+TGG 0.470545 6:+17761937 None:intergenic
CAATTCCATCTCAAGTTCAA+TGG 0.471344 6:-17761563 MsaT030986.1:CDS
GGTCACAAGTTCTTTGGCTG+AGG 0.486097 6:-17761780 MsaT030986.1:CDS
AGATAAGGTGATGTGTTTGA+TGG 0.498736 6:+17762087 None:intergenic
TCATCCAAAGGACTAAAAGA+AGG 0.506277 6:+17761613 None:intergenic
GGTTAAGTTTCTCTATAAGC+TGG 0.509495 6:+17761746 None:intergenic
GTGACCGACGTGCTGATTCG+CGG 0.510591 6:+17761836 None:intergenic
CAAACAAGAGAAGTAACAAC+AGG 0.515554 6:-17762126 MsaT030986.1:CDS
TGTGGACTATAGTTGAAGTC+AGG 0.518116 6:+17761955 None:intergenic
AAGATCCATTGAACTTGAGA+TGG 0.525838 6:+17761558 None:intergenic
AAGTCAGGAAATTGTTGATG+AGG 0.551877 6:+17761970 None:intergenic
CTTGTGACCAAAGCTCATCA+AGG 0.582135 6:+17761794 None:intergenic
AGTCAGGAAATTGTTGATGA+GGG 0.582558 6:+17761971 None:intergenic
CATCCAAAGGACTAAAAGAA+GGG 0.584492 6:+17761614 None:intergenic
CTAAGATATGTATCATCCAA+AGG 0.603956 6:+17761601 None:intergenic
CAGCACGTCGGTCACGCATG+AGG 0.614637 6:-17761828 MsaT030986.1:CDS
TCAATGAGAGGAAACACAGA+AGG 0.630812 6:-17761870 MsaT030986.1:CDS
ACCTTAGTCTCATCAATGAG+AGG 0.631783 6:-17761882 MsaT030986.1:CDS
GATCATGATTCTCAGAAACA+TGG 0.641220 6:+17761725 None:intergenic
GTCAGGAAATTGTTGATGAG+GGG 0.693054 6:+17761972 None:intergenic
CAAACCGCGAATCAGCACGT+CGG 0.801689 6:-17761840 MsaT030986.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 17761549 17762151 17761549 ID=MsaG030986
Chr6 mRNA 17761549 17762151 17761549 ID=MsaT030986.1;Parent=MsaG030986
Chr6 exon 17761549 17762151 17761549 ID=MsaT030986.1.exon1;Parent=MsaT030986.1
Chr6 CDS 17761549 17762151 17761549 ID=cds.MsaT030986.1;Parent=MsaT030986.1
Gene Sequence

>MsaT030986.1

ATGCAAACAAGAGAAGTAACAACAGGACTCAATTACATGCTACCATCAAACACATCACCTTATCTACAAAACTATAACAACATGATTCAAAATAACAACATTTCCTCATATCAACTCCAAAAATTCTCAAACCAATTATATGGTAATTACCTAAACACCCCTCATCAACAATTTCCTGACTTCAACTATAGTCCACAATCATCATGCATAAGCAGCAACTCAACTTCTGATGAAGCAGATGAACAAAACCTTAGTCTCATCAATGAGAGGAAACACAGAAGGATGATATCAAACCGCGAATCAGCACGTCGGTCACGCATGAGGAAGCAGAAACACCTTGATGAGCTTTGGTCACAAGTTCTTTGGCTGAGGAATGAAAATCACCAGCTTATAGAGAAACTTAACCATGTTTCTGAGAATCATGATCAAGTTGTTCAAGAGAATGCTCAGCTTAAAGAAGAAGCTTTGGAACTTCGACAAATGATAAAAGATATGCAGATTCATAGTCCTTTAATCCCTTCTTTTAGTCCTTTGGATGATACATATCTTAGAGATGATTCTTCAAACAATTCCATCTCAAGTTCAATGGATCTTCTTGGCTGA

Protein sequence

>MsaT030986.1

MQTREVTTGLNYMLPSNTSPYLQNYNNMIQNNNISSYQLQKFSNQLYGNYLNTPHQQFPDFNYSPQSSCISSNSTSDEADEQNLSLINERKHRRMISNRESARRSRMRKQKHLDELWSQVLWLRNENHQLIEKLNHVSENHDQVVQENAQLKEEALELRQMIKDMQIHSPLIPSFSPLDDTYLRDDSSNNSISSSMDLLG*