AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG031084

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG031084 MtrunA17_Chr6g0454781 98.795 83 0 1 1 82 1 83 1.09e-51 166
MsaG031084 MtrunA17_Chr7g0219461 64.835 91 22 2 2 82 1 91 7.20e-28 104
MsaG031084 MtrunA17_Chr4g0061561 71.930 57 16 0 26 82 35 91 8.74e-24 92.8
MsaG031084 MtrunA17_Chr3g0086961 71.667 60 15 1 25 82 32 91 1.61e-22 90.5
MsaG031084 MtrunA17_Chr8g0376571 80.000 45 9 0 38 82 51 95 1.14e-17 77.4
MsaG031084 MtrunA17_Chr2g0325171 59.649 57 20 1 26 82 5 58 1.85e-13 65.1
MsaG031084 MtrunA17_Chr4g0040921 59.649 57 20 1 26 82 3 56 1.87e-13 65.1
MsaG031084 MtrunA17_Chr8g0360441 68.293 41 13 0 42 82 48 88 1.78e-11 59.7
MsaG031084 MtrunA17_Chr2g0316391 71.795 39 11 0 44 82 16 54 6.71e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG031084 AT3G02150.1 80.769 52 10 0 31 82 57 108 8.53e-24 92.8
MsaG031084 AT3G02150.2 80.769 52 10 0 31 82 57 108 1.72e-23 93.2
MsaG031084 AT5G08070.2 65.574 61 18 1 22 82 10 67 2.16e-20 83.2
MsaG031084 AT5G08070.1 65.574 61 18 1 22 82 10 67 2.16e-20 83.2
MsaG031084 AT5G60970.1 75.510 49 12 0 34 82 49 97 4.36e-20 84.0
MsaG031084 AT1G30210.2 77.778 45 10 0 38 82 40 84 5.17e-17 75.1
MsaG031084 AT1G30210.1 77.778 45 10 0 38 82 40 84 5.17e-17 75.1
MsaG031084 AT4G18390.1 75.556 45 11 0 38 82 32 76 8.02e-15 69.3
MsaG031084 AT4G18390.2 75.556 45 11 0 38 82 32 76 8.02e-15 69.3
MsaG031084 AT2G31070.1 70.732 41 12 0 42 82 23 63 7.52e-12 60.8
MsaG031084 AT3G15030.2 68.293 41 13 0 42 82 39 79 3.57e-11 59.3
MsaG031084 AT3G15030.4 68.293 41 13 0 42 82 39 79 3.57e-11 59.3
MsaG031084 AT3G15030.3 68.293 41 13 0 42 82 39 79 3.57e-11 59.3
MsaG031084 AT3G15030.1 68.293 41 13 0 42 82 39 79 3.57e-11 59.3
MsaG031084 AT1G53230.1 68.293 41 13 0 42 82 43 83 6.77e-11 58.2
MsaG031084 AT1G53230.2 68.293 41 13 0 42 82 43 83 6.77e-11 58.2

Find 16 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGGAAATTCGGCTTCTTT+TGG 0.274258 6:+19909775 None:intergenic
AGCAAAGTATACACAATAAA+AGG 0.279500 6:-19909631 MsaT031084.1:CDS
CTAGACACCCTCACAATTCT+TGG 0.330306 6:+19909679 None:intergenic
TAAAAGGACTAAGAGATAGA+AGG 0.370333 6:-19909615 MsaT031084.1:CDS
CATAGAGGTGAATTGCTGTT+TGG 0.373931 6:+19909578 None:intergenic
GTGAGGGTGTCTAGAGCCTT+TGG 0.385600 6:-19909670 MsaT031084.1:CDS
GATGTAGTTGAATCATTTGA+TGG 0.415928 6:+19909736 None:intergenic
ACCTCTATGATCTTCAAGAT+AGG 0.446546 6:-19909564 MsaT031084.1:intron
TACTAAATGTGTGTTTGAAA+AGG 0.448199 6:-19908042 MsaT031084.1:intron
TGTCATTGAAAATGAGTACA+AGG 0.542534 6:-19908105 MsaT031084.1:CDS
AAAAGGACTAAGAGATAGAA+GGG 0.548162 6:-19909614 MsaT031084.1:CDS
ACCTATCTTGAAGATCATAG+AGG 0.598434 6:+19909563 None:intergenic
TAAAAGATCCAAGAATTGTG+AGG 0.608827 6:-19909687 MsaT031084.1:CDS
AAAAGATCCAAGAATTGTGA+GGG 0.611796 6:-19909686 MsaT031084.1:CDS
GTCATTGAAAATGAGTACAA+GGG 0.647352 6:-19908104 MsaT031084.1:CDS
CATCGTCAACAACATCGCAA+TGG 0.668784 6:-19909717 MsaT031084.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 19904186 19909809 19904186 ID=MsaG031084
Chr6 mRNA 19904186 19909809 19904186 ID=MsaT031084.1;Parent=MsaG031084
Chr6 exon 19904186 19904203 19904186 ID=MsaT031084.1.exon3;Parent=MsaT031084.1
Chr6 CDS 19904186 19904203 19904186 ID=cds.MsaT031084.1;Parent=MsaT031084.1
Chr6 exon 19908043 19908139 19908043 ID=MsaT031084.1.exon2;Parent=MsaT031084.1
Chr6 CDS 19908043 19908139 19908043 ID=cds.MsaT031084.1;Parent=MsaT031084.1
Chr6 exon 19909565 19909809 19909565 ID=MsaT031084.1.exon1;Parent=MsaT031084.1
Chr6 CDS 19909565 19909809 19909565 ID=cds.MsaT031084.1;Parent=MsaT031084.1
Gene Sequence

>MsaT031084.1

ATGATTAACATTCCAAAAGAAGCCGAATTTCCATTAAAACAAGAAGTTCTTCCATCAAATGATTCAACTACATCGTCAACAACATCGCAATGGTTAAAACTAAAAGATCCAAGAATTGTGAGGGTGTCTAGAGCCTTTGGAACAAAAGATAGACATAGCAAAGTATACACAATAAAAGGACTAAGAGATAGAAGGGTAAGACTTTCAATCCAAACAGCAATTCACCTCTATGATCTTCAAGATAGAGGCAAAGAAGATGTCATTGAAAATGAGTACAAGGGCAACCATTATTACCTTATCAAAAATCAAATGGACTTATCTACTAAATGTGTGTTTGAAAAGGAATATAGAGTCCATTAA
Protein sequence

>MsaT031084.1

MINIPKEAEFPLKQEVLPSNDSTTSSTTSQWLKLKDPRIVRVSRAFGTKDRHSKVYTIKGLRDRRVRLSIQTAIHLYDLQDRGKEDVIENEYKGNHYYLIKNQMDLSTKCVFEKEYRVH*