AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG031357

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG031357 MtrunA17_Chr3g0092621 70.787 89 26 0 6 94 67 155 1.00e-37 124
MsaG031357 MtrunA17_Chr5g0415191 78.378 74 16 0 23 96 102 175 1.25e-30 110
MsaG031357 MtrunA17_Chr2g0331031 68.571 70 22 0 23 92 109 178 1.30e-30 110
MsaG031357 MtrunA17_Chr4g0055931 73.134 67 18 0 24 90 96 162 1.70e-30 110
MsaG031357 MtrunA17_Chr6g0488471 70.769 65 19 0 23 87 97 161 5.13e-29 105
MsaG031357 MtrunA17_Chr4g0059701 90.000 70 7 0 23 92 84 153 1.14e-28 104
MsaG031357 MtrunA17_Chr4g0062741 67.164 67 22 0 23 89 138 204 9.47e-27 99.8
MsaG031357 MtrunA17_Chr8g0389241 67.692 65 20 1 23 87 105 168 1.10e-26 99.0
MsaG031357 MtrunA17_Chr1g0162081 65.672 67 23 0 23 89 122 188 2.00e-25 96.3
MsaG031357 MtrunA17_Chr1g0210471 66.667 66 22 0 24 89 125 190 2.37e-25 94.7
MsaG031357 MtrunA17_Chr5g0429521 65.672 67 23 0 23 89 132 198 2.83e-25 95.9
MsaG031357 MtrunA17_Chr3g0104071 65.672 67 23 0 23 89 147 213 3.23e-25 95.9
MsaG031357 MtrunA17_Chr7g0241761 65.152 66 23 0 23 88 140 205 2.12e-24 93.6
MsaG031357 MtrunA17_Chr6g0452791 71.667 60 17 0 23 82 80 139 6.72e-24 89.4
MsaG031357 MtrunA17_Chr5g0436501 61.111 72 26 1 23 92 125 196 1.23e-22 88.2
MsaG031357 MtrunA17_Chr3g0135671 53.750 80 31 2 23 99 91 167 7.67e-22 85.5
MsaG031357 MtrunA17_Chr5g0409841 49.275 69 33 1 22 88 91 159 1.33e-17 74.3
MsaG031357 MtrunA17_Chr5g0440741 45.570 79 37 2 25 97 102 180 5.25e-17 73.6
MsaG031357 MtrunA17_Chr5g0440731 44.444 81 39 2 23 97 105 185 2.24e-16 72.0
MsaG031357 MtrunA17_Chr5g0409831 46.835 79 36 2 21 93 75 153 1.90e-15 68.6
MsaG031357 MtrunA17_Chr8g0365011 41.667 96 42 2 7 88 76 171 9.95e-15 67.4
MsaG031357 MtrunA17_Chr8g0364731 55.932 59 24 1 32 88 10 68 1.20e-13 63.2
MsaG031357 MtrunA17_Chr3g0113121 45.833 72 32 2 25 89 97 168 5.77e-12 59.3
MsaG031357 MtrunA17_Chr1g0192061 38.776 98 49 4 4 91 35 131 3.55e-11 56.6
MsaG031357 MtrunA17_Chr5g0432921 48.438 64 29 2 25 85 115 177 8.93e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG031357 AT5G47390.1 75.362 69 17 0 24 92 97 165 5.22e-32 115
MsaG031357 AT1G49010.1 63.158 76 28 0 23 98 134 209 7.50e-28 103
MsaG031357 AT1G70000.1 77.143 70 16 0 24 93 99 168 4.51e-27 100
MsaG031357 AT1G70000.2 77.143 70 16 0 24 93 99 168 4.51e-27 100
MsaG031357 AT5G56840.1 54.023 87 37 2 4 89 74 158 6.40e-26 96.7
MsaG031357 AT2G38090.1 65.672 67 23 0 23 89 141 207 1.20e-25 97.1
MsaG031357 AT5G04760.1 66.667 66 22 0 24 89 100 165 1.44e-25 95.1
MsaG031357 AT1G74840.2 80.282 71 14 0 23 93 96 166 1.53e-25 95.5
MsaG031357 AT5G58900.1 65.672 67 23 0 23 89 141 207 1.59e-25 96.7
MsaG031357 AT1G74840.1 80.282 71 14 0 23 93 96 166 2.01e-25 95.9
MsaG031357 AT5G08520.1 64.179 67 24 0 23 89 118 184 8.41e-25 94.7
MsaG031357 AT3G16350.1 67.606 71 23 0 23 93 137 207 9.34e-25 95.9
MsaG031357 AT1G19000.2 78.571 70 15 0 23 92 102 171 1.54e-24 94.0
MsaG031357 AT1G19000.1 78.571 70 15 0 23 92 102 171 1.54e-24 94.0
MsaG031357 AT5G61620.1 59.701 67 27 0 24 90 109 175 1.14e-23 92.0
MsaG031357 AT5G01200.1 61.194 67 26 0 23 89 146 212 9.08e-23 89.0
MsaG031357 AT3G11280.2 57.746 71 30 0 23 93 127 197 1.41e-22 88.6
MsaG031357 AT3G11280.1 57.746 71 30 0 23 93 127 197 1.41e-22 88.6
MsaG031357 AT5G05790.2 61.194 67 26 0 23 89 131 197 2.44e-22 88.2
MsaG031357 AT5G05790.1 61.194 67 26 0 23 89 131 197 2.44e-22 88.2
MsaG031357 AT3G10580.2 54.545 66 28 1 23 88 95 158 7.15e-18 75.9
MsaG031357 AT4G09450.1 50.704 71 35 0 23 93 91 161 7.69e-18 74.7
MsaG031357 AT3G10580.1 54.545 66 28 1 23 88 95 158 1.10e-17 75.9
MsaG031357 AT3G10590.1 49.275 69 34 1 24 92 112 179 3.40e-17 73.2
MsaG031357 AT5G23650.1 54.545 66 30 0 23 88 120 185 6.22e-17 74.3

Find 23 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGAATTATTACTCGAAA+GGG 0.416760 6:-24603320 None:intergenic
AAGCTTAAAAGTTGTTGAAA+AGG 0.425063 6:+24602924 MsaT031357.1:CDS
TTGGTTGGATGGCAGAAAGT+TGG 0.429282 6:+24605278 MsaT031357.1:CDS
TGTTTCTACAAAGAGATTGC+CGG 0.433250 6:-24605461 None:intergenic
AGAAAGTTGGAAAAGGTGAT+AGG 0.454745 6:+24605291 MsaT031357.1:CDS
TTATTACTCGAAAGGGGTTG+AGG 0.501025 6:-24603313 None:intergenic
AGTGGTGATATCATAGAGAC+TGG 0.518743 6:-24605423 None:intergenic
TTGTGCTCTTCCTCCGTCCA+CGG 0.535860 6:-24605248 None:intergenic
ATCAAGAATTATTACTCGAA+AGG 0.542285 6:-24603321 None:intergenic
GGATGGCAGAAAGTTGGAAA+AGG 0.556224 6:+24605284 MsaT031357.1:CDS
TGGATCAGGGATTCCGTGGA+CGG 0.564976 6:+24605235 MsaT031357.1:intron
GACGGCGATTGAGATTACTT+TGG 0.589287 6:-24605391 None:intergenic
TATCATAGAGACTGGATCTA+CGG 0.592657 6:-24605415 None:intergenic
CTATGATATCACCACTGACA+CGG 0.608553 6:+24605430 MsaT031357.1:CDS
GTTGGAAAAGGTGATAGGAG+AGG 0.613085 6:+24605296 MsaT031357.1:CDS
GCATGACTTGCAACCTGCGT+AGG 0.620867 6:-24605350 None:intergenic
GTGATAGGAGAGGAATCTCA+AGG 0.624002 6:+24605306 MsaT031357.1:CDS
TGAATGGATCAGGGATTCCG+TGG 0.629323 6:+24605231 MsaT031357.1:intron
CAAGAATTATTACTCGAAAG+GGG 0.629484 6:-24603319 None:intergenic
GAAAACAAGAACGCCTACGC+AGG 0.639644 6:+24605337 MsaT031357.1:CDS
TAAACTTCAGATATGACACA+TGG 0.660343 6:+24602889 None:intergenic
ATCAGGGATTCCGTGGACGG+AGG 0.665588 6:+24605238 MsaT031357.1:intron
AGAGACTGGATCTACGGCGA+CGG 0.700084 6:-24605409 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 24602901 24605478 24602901 ID=MsaG031357
Chr6 mRNA 24602901 24605478 24602901 ID=MsaT031357.1;Parent=MsaG031357
Chr6 exon 24602901 24602945 24602901 ID=MsaT031357.1.exon1;Parent=MsaT031357.1
Chr6 CDS 24602901 24602945 24602901 ID=cds.MsaT031357.1;Parent=MsaT031357.1
Chr6 exon 24603310 24603328 24603310 ID=MsaT031357.1.exon2;Parent=MsaT031357.1
Chr6 CDS 24603310 24603328 24603310 ID=cds.MsaT031357.1;Parent=MsaT031357.1
Chr6 exon 24605243 24605478 24605243 ID=MsaT031357.1.exon3;Parent=MsaT031357.1
Chr6 CDS 24605243 24605478 24605243 ID=cds.MsaT031357.1;Parent=MsaT031357.1
Gene Sequence

>MsaT031357.1

ATGACACATGGAGTGATTTCCAAAAGCTTAAAAGTTGTTGAAAAGATTCCTCAACCCCTTTCGAGGATTCCGTGGACGGAGGAAGAGCACAAGATGTTTTTGGTTGGATGGCAGAAAGTTGGAAAAGGTGATAGGAGAGGAATCTCAAGGAACTATGTGAAAACAAGAACGCCTACGCAGGTTGCAAGTCATGCTCAGAAGTACTCTCTGAACCAAAGTAATCTCAATCGCCGTCGCCGTAGATCCAGTCTCTATGATATCACCACTGACACGGTAATTTTTCCGGCAATCTCTTTGTAG
Protein sequence

>MsaT031357.1

MTHGVISKSLKVVEKIPQPLSRIPWTEEEHKMFLVGWQKVGKGDRRGISRNYVKTRTPTQVASHAQKYSLNQSNLNRRRRRSSLYDITTDTVIFPAISL*