AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG031633

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG031633 MtrunA17_Chr6g0470211 80.645 155 19 2 1 144 27 181 5.36e-72 214
MsaG031633 MtrunA17_Chr5g0438861 88.235 85 10 0 1 85 184 268 2.57e-46 155
MsaG031633 MtrunA17_Chr5g0438861 81.967 61 11 0 84 144 343 403 6.32e-25 98.6
MsaG031633 MtrunA17_Chr3g0144301 73.077 104 9 1 1 85 215 318 1.82e-42 146
MsaG031633 MtrunA17_Chr3g0144301 85.246 61 9 0 84 144 393 453 1.68e-26 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG031633 AT2G33610.1 54.795 73 33 0 1 73 210 282 2.74e-21 89.0

Find 26 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAGCTATTACAAATTTC+GGG 0.188443 6:+31226811 MsaT031633.1:CDS
CTTGAAGCTATTACAAATTT+CGG 0.191024 6:+31226810 MsaT031633.1:CDS
TTTACCCGACACAACAGTTC+CGG 0.405508 6:-31226955 None:intergenic
TGAGGATTTAGACATGCTAA+TGG 0.411250 6:+31227321 MsaT031633.1:CDS
AGGGTTGCTCATCAAGTTGT+TGG 0.467288 6:+31226846 MsaT031633.1:CDS
GATTCACATTCAGCATCAAC+AGG 0.484400 6:-31226933 None:intergenic
TTGAACGCAATAGAGATCCT+TGG 0.491873 6:-31227201 None:intergenic
TTCAAATTTACATCTTGAGA+TGG 0.514708 6:+31227219 MsaT031633.1:CDS
CAAAATCTATAAGCTTGTCC+TGG 0.515190 6:-31227300 None:intergenic
GTTGATGCTGAATGTGAATC+CGG 0.520833 6:+31226936 MsaT031633.1:CDS
ACGAGCAACACATTCCTTGT+CGG 0.529759 6:-31226874 None:intergenic
GGCTTCAACTGATCGCCAAA+AGG 0.531669 6:-31226909 None:intergenic
TGATTACCTTCTCCAACTGC+TGG 0.553911 6:-31227354 None:intergenic
TTGCTCATCAAGTTGTTGGT+AGG 0.563248 6:+31226850 MsaT031633.1:CDS
TACCGATCAGATTCTATCCA+AGG 0.581696 6:+31227184 MsaT031633.1:intron
CAAGAGAGTGGAGATAAGTG+AGG 0.590112 6:+31226746 MsaT031633.1:CDS
TAATTCTGAATTCAAGAGAG+TGG 0.590146 6:+31226734 MsaT031633.1:CDS
AGAACGCCAGCAGTTGGAGA+AGG 0.591371 6:+31227348 MsaT031633.1:CDS
GAATCCGGAACTGTTGTGTC+GGG 0.600124 6:+31226951 MsaT031633.1:CDS
TGAGATGGAAGAATCAGATG+TGG 0.605895 6:+31227234 MsaT031633.1:CDS
TTTCGGGGATGATTCAAAGA+GGG 0.618882 6:+31226827 MsaT031633.1:CDS
TGAAGCTATTACAAATTTCG+GGG 0.621595 6:+31226812 MsaT031633.1:CDS
TGAATCCGGAACTGTTGTGT+CGG 0.643345 6:+31226950 MsaT031633.1:CDS
ATTTCGGGGATGATTCAAAG+AGG 0.645867 6:+31226826 MsaT031633.1:CDS
AGTTGTTGGTAGGACCGACA+AGG 0.658858 6:+31226860 MsaT031633.1:CDS
TCACATTCAGCATCAACAGG+AGG 0.659217 6:-31226930 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 31226729 31227381 31226729 ID=MsaG031633
Chr6 mRNA 31226729 31227381 31226729 ID=MsaT031633.1;Parent=MsaG031633
Chr6 exon 31226729 31226981 31226729 ID=MsaT031633.1.exon1;Parent=MsaT031633.1
Chr6 CDS 31226729 31226981 31226729 ID=cds.MsaT031633.1;Parent=MsaT031633.1
Chr6 exon 31227194 31227381 31227194 ID=MsaT031633.1.exon2;Parent=MsaT031633.1
Chr6 CDS 31227194 31227381 31227194 ID=cds.MsaT031633.1;Parent=MsaT031633.1
Gene Sequence

>MsaT031633.1

ATGAGTAATTCTGAATTCAAGAGAGTGGAGATAAGTGAGGAGACAAAAAATGTATGGACTGAGGAGGAAACACTAAATCTTCTTGAAGCTATTACAAATTTCGGGGATGATTCAAAGAGGGTTGCTCATCAAGTTGTTGGTAGGACCGACAAGGAATGTGTTGCTCGTTTTCTAGAGCTTCCTTTTGGCGATCAGTTGAAGCCTCCTGTTGATGCTGAATGTGAATCCGGAACTGTTGTGTCGGGTAAATCTAATTCTATCCAAGGATCTCTATTGCGTTCAAATTTACATCTTGAGATGGAAGAATCAGATGTGGAAAAAGCTATTTCTGAGGTTATAGAAGTTCAGATGAAAAATATCCAGGACAAGCTTATAGATTTTGAGGATTTAGACATGCTAATGGAAAAAGAACGCCAGCAGTTGGAGAAGGTAATCATGTAA
Protein sequence

>MsaT031633.1

MSNSEFKRVEISEETKNVWTEEETLNLLEAITNFGDDSKRVAHQVVGRTDKECVARFLELPFGDQLKPPVDAECESGTVVSGKSNSIQGSLLRSNLHLEMEESDVEKAISEVIEVQMKNIQDKLIDFEDLDMLMEKERQQLEKVIM*