AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG031642

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG031642 MtrunA17_Chr6g0470151 84.254 362 47 1 1 362 1 352 1.33e-164 473
MsaG031642 MtrunA17_Chr2g0289181 47.210 233 123 0 327 559 1 233 2.21e-69 223
MsaG031642 MtrunA17_Chr6g0470161 79.200 125 21 1 435 559 1 120 1.01e-61 199
MsaG031642 MtrunA17_Chr7g0266611 50.303 165 81 1 395 559 2 165 4.25e-35 129
MsaG031642 MtrunA17_Chr8g0366921 51.337 187 72 5 1 180 1 175 6.84e-30 119
MsaG031642 MtrunA17_Chr1g0202461 39.200 125 65 2 291 415 222 335 1.26e-24 105
MsaG031642 MtrunA17_Chr7g0266621 37.685 337 159 7 1 321 1 302 3.02e-23 100
MsaG031642 MtrunA17_Chr8g0348311 45.361 97 51 1 317 413 134 228 2.81e-21 95.5
MsaG031642 MtrunA17_Chr7g0248691 41.818 110 56 1 312 413 118 227 1.81e-20 92.8
MsaG031642 MtrunA17_Chr7g0270051 45.977 87 47 0 333 419 46 132 3.44e-20 90.5
MsaG031642 MtrunA17_Chr8g0387501 42.308 104 52 2 341 439 130 230 1.87e-17 82.8
MsaG031642 MtrunA17_Chr2g0293481 46.753 77 40 1 341 417 268 343 5.07e-17 84.0
MsaG031642 MtrunA17_Chr7g0238921 41.509 106 50 3 292 397 87 180 6.96e-17 82.0
MsaG031642 MtrunA17_Chr5g0401821 41.414 99 55 1 316 411 172 270 3.45e-16 79.0
MsaG031642 MtrunA17_Chr4g0042831 40.860 93 50 2 338 426 276 367 2.20e-15 78.2
MsaG031642 MtrunA17_Chr1g0184691 45.122 82 42 1 321 399 271 352 2.23e-15 77.8
MsaG031642 MtrunA17_Chr4g0053281 47.887 71 37 0 341 411 267 337 2.49e-15 77.4
MsaG031642 MtrunA17_Chr4g0071521 43.529 85 45 1 316 397 218 302 2.54e-15 77.0
MsaG031642 MtrunA17_Chr2g0293901 43.407 182 87 5 1 181 1 167 2.62e-15 75.1
MsaG031642 MtrunA17_Chr2g0319961 50.794 63 31 0 341 403 81 143 2.95e-15 76.3
MsaG031642 MtrunA17_Chr8g0379461 47.619 63 33 0 341 403 77 139 3.97e-15 75.5
MsaG031642 MtrunA17_Chr2g0284321 50.000 60 30 0 341 400 36 95 5.22e-15 74.3
MsaG031642 MtrunA17_Chr8g0387981 38.043 92 51 2 310 395 98 189 2.78e-14 74.7
MsaG031642 MtrunA17_Chr7g0218081 43.939 66 36 1 338 403 408 472 5.11e-14 74.7
MsaG031642 MtrunA17_Chr7g0218081 43.077 65 35 2 336 400 224 286 8.92e-11 64.3
MsaG031642 MtrunA17_Chr1g0147881 34.694 98 58 2 320 411 237 334 6.09e-14 73.2
MsaG031642 MtrunA17_Chr7g0228941 42.647 68 39 0 334 401 46 113 6.10e-14 68.9
MsaG031642 MtrunA17_Chr3g0139021 37.778 90 55 1 309 397 282 371 9.72e-14 73.2
MsaG031642 MtrunA17_Chr5g0418191 41.026 78 45 1 338 415 318 394 1.16e-13 73.2
MsaG031642 MtrunA17_Chr8g0368721 46.809 188 84 4 1 187 1 173 1.54e-13 71.6
MsaG031642 MtrunA17_Chr3g0136961 39.048 105 60 2 315 418 112 213 1.52e-12 68.6
MsaG031642 MtrunA17_Chr2g0302391 37.778 90 51 1 341 425 212 301 1.83e-12 69.3
MsaG031642 MtrunA17_Chr7g0249311 46.377 69 35 2 332 400 171 237 3.20e-12 69.3
MsaG031642 MtrunA17_Chr7g0249311 39.744 78 40 2 340 411 392 468 3.84e-12 68.9
MsaG031642 MtrunA17_Chr7g0263531 42.105 76 41 2 341 416 362 434 4.82e-12 68.2
MsaG031642 MtrunA17_Chr3g0129321 39.130 69 39 3 337 404 115 181 5.08e-12 65.5
MsaG031642 MtrunA17_Chr4g0074001 30.702 114 73 3 291 403 313 421 5.11e-12 68.6
MsaG031642 MtrunA17_Chr4g0051881 45.902 61 32 1 340 400 178 237 6.21e-12 67.0
MsaG031642 MtrunA17_Chr3g0079691 50.000 62 29 2 339 400 279 338 7.13e-12 68.2
MsaG031642 MtrunA17_Chr3g0079691 38.806 67 40 1 340 406 495 560 2.44e-11 66.6
MsaG031642 MtrunA17_Chr5g0443131 46.774 62 32 1 340 401 157 217 8.49e-12 66.6
MsaG031642 MtrunA17_Chr7g0243891 48.529 68 33 2 333 400 209 274 1.11e-11 67.4
MsaG031642 MtrunA17_Chr7g0249301 42.188 64 36 1 340 403 292 354 1.39e-11 66.6
MsaG031642 MtrunA17_Chr4g0001361 43.939 66 37 0 335 400 157 222 1.63e-11 65.9
MsaG031642 MtrunA17_Chr5g0445311 38.806 67 40 1 340 406 364 429 2.10e-11 66.6
MsaG031642 MtrunA17_Chr3g0101131 29.341 167 98 6 254 406 320 480 2.24e-11 66.6
MsaG031642 MtrunA17_Chr2g0288871 33.010 103 65 3 299 400 266 365 3.24e-11 65.9
MsaG031642 MtrunA17_Chr3g0099641 36.145 83 50 2 318 400 232 311 5.88e-11 65.1
MsaG031642 MtrunA17_Chr2g0333851 43.750 64 36 0 338 401 167 230 6.31e-11 63.9
MsaG031642 MtrunA17_Chr1g0194481 44.262 61 33 1 340 400 158 217 9.96e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG031642 AT5G52830.1 48.980 98 48 2 318 415 144 239 6.31e-23 100
MsaG031642 AT4G01250.1 35.099 151 92 2 284 430 74 222 3.80e-21 94.7
MsaG031642 AT4G23550.1 44.186 86 47 1 332 417 132 216 8.06e-18 85.1
MsaG031642 AT1G30650.1 35.333 150 93 1 294 443 175 320 2.50e-17 85.1
MsaG031642 AT5G28650.1 41.304 92 51 1 311 399 230 321 3.53e-17 83.6
MsaG031642 AT3G04670.1 36.441 118 61 3 285 399 215 321 4.21e-17 83.2
MsaG031642 AT5G45050.1 40.816 98 57 1 300 397 1141 1237 8.63e-17 84.7
MsaG031642 AT5G45050.4 40.816 98 57 1 300 397 1002 1098 8.85e-17 84.7
MsaG031642 AT5G45050.2 40.816 98 57 1 300 397 1113 1209 9.85e-17 84.7
MsaG031642 AT2G23320.1 43.529 85 45 1 316 397 213 297 1.47e-16 81.3
MsaG031642 AT2G34830.1 55.000 60 27 0 341 400 216 275 1.48e-16 82.4
MsaG031642 AT2G34830.2 55.000 60 27 0 341 400 225 284 1.71e-16 82.4
MsaG031642 AT4G24240.1 42.353 85 46 1 316 397 254 338 2.35e-15 78.2
MsaG031642 AT4G31550.2 38.000 100 59 1 309 405 211 310 1.17e-14 75.9
MsaG031642 AT4G31550.1 38.710 93 54 1 316 405 219 311 1.79e-14 75.1
MsaG031642 AT3G58710.2 46.970 66 35 0 341 406 71 136 2.54e-14 73.9
MsaG031642 AT3G58710.1 46.970 66 35 0 341 406 72 137 2.64e-14 73.9
MsaG031642 AT2G24570.1 39.080 87 50 1 316 399 216 302 3.55e-14 74.3
MsaG031642 AT1G29280.1 48.333 60 31 0 341 400 75 134 6.39e-14 72.4
MsaG031642 AT2G03340.1 41.558 77 44 1 338 414 413 488 1.10e-13 73.9
MsaG031642 AT2G30590.1 39.080 87 50 1 316 399 286 372 2.66e-13 72.0
MsaG031642 AT1G80840.1 39.583 96 53 2 340 432 146 239 4.11e-13 70.9
MsaG031642 AT5G43290.1 41.096 73 42 1 325 397 99 170 8.73e-13 69.3
MsaG031642 AT3G01080.1 42.857 63 35 1 338 400 304 365 1.43e-12 70.1
MsaG031642 AT3G01080.2 36.559 93 48 2 318 400 312 403 1.48e-12 70.5
MsaG031642 AT4G26640.7 41.026 78 43 3 338 414 169 244 2.32e-12 70.1
MsaG031642 AT4G26640.5 41.026 78 43 3 338 414 33 108 2.59e-12 69.3
MsaG031642 AT4G26640.6 41.026 78 43 3 338 414 33 108 2.59e-12 69.3
MsaG031642 AT4G26640.4 41.026 78 43 3 338 414 33 108 2.59e-12 69.3
MsaG031642 AT4G26640.3 41.026 78 43 3 338 414 33 108 2.59e-12 69.3
MsaG031642 AT4G26640.1 41.026 78 43 3 338 414 137 212 2.78e-12 69.7
MsaG031642 AT4G26640.2 41.026 78 43 3 338 414 209 284 2.85e-12 69.7
MsaG031642 AT2G04880.2 44.444 63 34 1 341 403 284 345 3.68e-12 68.9
MsaG031642 AT5G45260.1 28.834 163 92 7 247 403 1129 1273 3.79e-12 70.1
MsaG031642 AT2G04880.1 44.444 63 34 1 341 403 308 369 3.97e-12 68.9
MsaG031642 AT5G07100.1 46.970 66 34 1 335 400 112 176 4.74e-12 67.8
MsaG031642 AT5G07100.2 46.970 66 34 1 335 400 19 83 5.63e-12 65.9
MsaG031642 AT5G07100.4 46.970 66 34 1 335 400 19 83 5.63e-12 65.9
MsaG031642 AT5G07100.5 46.970 66 34 1 335 400 19 83 5.63e-12 65.9
MsaG031642 AT5G07100.3 46.970 66 34 1 335 400 19 83 5.63e-12 65.9
MsaG031642 AT1G13960.2 42.857 63 35 1 338 400 254 315 9.80e-12 67.4
MsaG031642 AT1G13960.1 42.857 63 35 1 338 400 407 468 9.87e-12 67.8
MsaG031642 AT2G47260.1 38.636 88 52 2 313 400 148 233 1.43e-11 66.6
MsaG031642 AT4G18170.1 35.644 101 59 2 302 397 129 228 2.72e-11 65.5
MsaG031642 AT2G37260.1 37.037 81 46 2 321 397 326 405 2.93e-11 66.2
MsaG031642 AT2G37260.3 37.037 81 46 2 321 397 246 325 3.30e-11 65.5
MsaG031642 AT2G37260.2 37.037 81 46 2 321 397 244 323 3.34e-11 65.5
MsaG031642 AT1G29860.1 43.548 62 34 1 340 401 136 196 4.38e-11 64.3
MsaG031642 AT2G25000.2 45.902 61 33 0 340 400 103 163 4.99e-11 63.5
MsaG031642 AT2G25000.4 45.902 61 33 0 340 400 103 163 4.99e-11 63.5
MsaG031642 AT2G25000.3 45.902 61 33 0 340 400 125 185 5.54e-11 63.5
MsaG031642 AT5G56270.1 39.062 64 38 1 340 403 487 549 6.10e-11 65.9
MsaG031642 AT2G21900.1 45.161 62 33 1 340 401 109 169 6.12e-11 62.4
MsaG031642 AT2G46400.1 38.710 93 50 2 310 395 69 161 6.66e-11 63.9

Find 145 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTAGGAAACAAACTTAT+TGG 0.150181 6:-31336264 MsaT031642.1:CDS
TTTCTCTAGCAGCGGGTAAA+TGG 0.194770 6:+31336400 None:intergenic
TACCCGCTGTTGGAAATTTC+AGG 0.250971 6:-31336557 MsaT031642.1:CDS
AGTGGTGGTGGTTGGGATGG+GGG 0.259814 6:+31338555 None:intergenic
TGTGATTGATTGAGCTTTCT+TGG 0.276762 6:+31337989 None:intergenic
TTGAGGAGATATGACGTTAT+TGG 0.276825 6:+31338717 None:intergenic
TAGTGGTGGTGGTTGGGATG+GGG 0.282225 6:+31338554 None:intergenic
GGTGGGAGTATTGAAATTAT+TGG 0.287618 6:+31338291 None:intergenic
TGGTGGTCGTGGACAAAATA+TGG 0.290490 6:-31337723 MsaT031642.1:CDS
TAGTCTTTGATGACTTGTTC+TGG 0.291346 6:+31336583 None:intergenic
TATGTCAGAGGATTTCTTAT+TGG 0.311118 6:-31336196 MsaT031642.1:CDS
TGTAATGTGGGTTGGGATAG+TGG 0.316848 6:+31338537 None:intergenic
ATAGTGGTGGTGGTTGGGAT+GGG 0.318096 6:+31338553 None:intergenic
GTGGTGGTGCAGGGGCTAAT+AGG 0.332725 6:+31338169 None:intergenic
TTGGGATAGTGGTGGTGGTT+GGG 0.338841 6:+31338548 None:intergenic
AACATTATCACTAGTGTTCA+TGG 0.339452 6:-31338136 MsaT031642.1:CDS
GATAGTGGTGGTGGTTGGGA+TGG 0.340741 6:+31338552 None:intergenic
CTAAAACCATCAAAGTAAAA+TGG 0.342350 6:+31338685 None:intergenic
TTGGTAGGGGTGGGGTTAAC+AGG 0.344695 6:+31338310 None:intergenic
TAGTGGTTGTTGAAGGGGTT+TGG 0.351398 6:+31338485 None:intergenic
GTGATTGATTGAGCTTTCTT+GGG 0.361329 6:+31337990 None:intergenic
GGTGGAAGGAGTAGTGAGGG+TGG 0.366407 6:+31338273 None:intergenic
CAAATAAGCTTTCAGAAGAT+TGG 0.368905 6:-31337743 MsaT031642.1:CDS
AGTCTTTGATGACTTGTTCT+GGG 0.370924 6:+31336584 None:intergenic
AGTCAAGTTGGTGGTGGTGC+AGG 0.372354 6:+31338159 None:intergenic
GAATCACAAATCCTGCCTTT+AGG 0.375324 6:-31336279 MsaT031642.1:CDS
TTGGTGTTTGAGATGGAGAT+GGG 0.375368 6:+31338764 None:intergenic
GTTGGGATAGTGGTGGTGGT+TGG 0.384329 6:+31338547 None:intergenic
GGGTTTGGATAATAGTGATT+GGG 0.385706 6:+31338500 None:intergenic
CCGGGAATAGTGGTTGTTGA+AGG 0.405815 6:+31338478 None:intergenic
AATTAACAACAAGGTAACTT+TGG 0.407679 6:-31337781 MsaT031642.1:CDS
ATGGAGAGAGGTGGTGGGAT+TGG 0.408785 6:+31338579 None:intergenic
AATTACCCAACACCAGATCT+AGG 0.412137 6:-31337961 MsaT031642.1:CDS
AAGGAACAGGGTTATTGTCT+TGG 0.412589 6:+31338656 None:intergenic
TGAAATTATTGGTAGGGGTG+GGG 0.414985 6:+31338302 None:intergenic
CCTTCAACAACCACTATTCC+CGG 0.416292 6:-31338478 MsaT031642.1:CDS
GAACCCAGCTTTGAAATGTT+TGG 0.416902 6:+31336528 None:intergenic
GTGATTCACCGGAGTAAACC+TGG 0.417652 6:+31336295 None:intergenic
CAAAACATAATTCAAGAATC+AGG 0.420705 6:-31337910 MsaT031642.1:intron
CAACAAGAAATGCAATCAAA+TGG 0.425635 6:-31338367 MsaT031642.1:CDS
AACACTTGATTGTTATTGTT+TGG 0.427124 6:+31338052 None:intergenic
TTCTCTAGCAGCGGGTAAAT+GGG 0.434011 6:+31336401 None:intergenic
CCAATAAGTTTGTTTCCTAA+AGG 0.437137 6:+31336264 None:intergenic
AGGAACAGGGTTATTGTCTT+GGG 0.439157 6:+31338657 None:intergenic
TCCAATGGACATTGACGATT+GGG 0.439428 6:-31338854 None:intergenic
GTCAAGTTGGTGGTGGTGCA+GGG 0.440160 6:+31338160 None:intergenic
CACAACAAAAGTGTCCTCTT+TGG 0.441667 6:+31336671 None:intergenic
GCAGGTAAATGGAACTCTAT+TGG 0.442204 6:-31336615 MsaT031642.1:CDS
ATTCCCGGCCACATAACATT+TGG 0.443207 6:-31338463 MsaT031642.1:CDS
CTAAGTTCAGTTTCTGAATG+AGG 0.443490 6:+31336429 None:intergenic
TTGAAATTATTGGTAGGGGT+GGG 0.444762 6:+31338301 None:intergenic
GTCACCAAACATTTCAAAGC+TGG 0.445873 6:-31336532 MsaT031642.1:CDS
CGGGAATAGTGGTTGTTGAA+GGG 0.449477 6:+31338479 None:intergenic
ACTTGAATATTCCTGCTCTC+TGG 0.454557 6:+31336474 None:intergenic
GTTGGTGTTTGAGATGGAGA+TGG 0.454612 6:+31338763 None:intergenic
GTCATGGCGGTAATTCTAGT+AGG 0.454681 6:+31338250 None:intergenic
TGATTGTTGTAATGTGGGTT+GGG 0.462715 6:+31338530 None:intergenic
AGTGATAATGTTAGTCAAGT+TGG 0.462717 6:+31338147 None:intergenic
AGATATGACGTTATTGGTGA+TGG 0.464263 6:+31338723 None:intergenic
TAAGTTTGTTTCCTAAAGGC+AGG 0.465057 6:+31336268 None:intergenic
GGGGTTTGGATAATAGTGAT+TGG 0.471326 6:+31338499 None:intergenic
ATTAGGTAAAATCCTAGATC+TGG 0.473232 6:+31337949 None:intergenic
GGAGTATTGAAATTATTGGT+AGG 0.476280 6:+31338295 None:intergenic
CTCCAATGGACATTGACGAT+TGG 0.476413 6:-31338855 None:intergenic
TCTGACATATCAGTCATGTT+TGG 0.477660 6:+31336210 None:intergenic
ACCTGGGCATTGCTACTCAT+TGG 0.480044 6:+31336312 None:intergenic
ATAGAGTTCCATTTACCTGC+TGG 0.487185 6:+31336618 None:intergenic
GTGGGTTGGGATAGTGGTGG+TGG 0.487824 6:+31338543 None:intergenic
TGAAGTGATTGTTGTAATGT+GGG 0.489359 6:+31338525 None:intergenic
GAGTATTGAAATTATTGGTA+GGG 0.491396 6:+31338296 None:intergenic
TGAGGGATTTCTGATTGACT+AGG 0.496461 6:+31336447 None:intergenic
GTTAGCGAAGGCGTTGGTAG+TGG 0.498129 6:+31338228 None:intergenic
ATGAAGTGATTGTTGTAATG+TGG 0.498474 6:+31338524 None:intergenic
CTAATAGGTGCAGTGGTGGT+CGG 0.503498 6:+31338184 None:intergenic
AACAAGAAATGCAATCAAAT+GGG 0.503558 6:-31338366 MsaT031642.1:CDS
TAAGTTCAGTTTCTGAATGA+GGG 0.506377 6:+31336430 None:intergenic
TGTTATGTGGCCGGGAATAG+TGG 0.508241 6:+31338468 None:intergenic
GGGAATAGTGGTTGTTGAAG+GGG 0.513196 6:+31338480 None:intergenic
TCTTGTTGTTCGATAAGTGT+TGG 0.517204 6:+31338382 None:intergenic
TCACCAAACATTTCAAAGCT+GGG 0.518423 6:-31336531 MsaT031642.1:CDS
GTGATTGTTGTAATGTGGGT+TGG 0.519681 6:+31338529 None:intergenic
GATTTAATTTCTCTAGCAGC+GGG 0.519918 6:+31336393 None:intergenic
TCATGGCGGTAATTCTAGTA+GGG 0.520929 6:+31338251 None:intergenic
AGTATTGAAATTATTGGTAG+GGG 0.523845 6:+31338297 None:intergenic
AAAATCCTAGATCTGGTGTT+GGG 0.524682 6:+31337956 None:intergenic
AAGACTAGATTACCCGCTGT+TGG 0.528458 6:-31336567 MsaT031642.1:CDS
ACAGTGGTTGGTGTTTGAGA+TGG 0.528977 6:+31338757 None:intergenic
GAGTAATGCATAATCTGAAA+TGG 0.529216 6:+31338096 None:intergenic
TAGGGGTGGAAGGAGTAGTG+AGG 0.529334 6:+31338269 None:intergenic
AGGCAGGATTTGTGATTCAC+CGG 0.532516 6:+31336284 None:intergenic
ATTGAAATTATTGGTAGGGG+TGG 0.533722 6:+31338300 None:intergenic
GTACCAAATGTTATGTGGCC+GGG 0.534649 6:+31338460 None:intergenic
TCCCAATCGTCAATGTCCAT+TGG 0.539210 6:+31338853 None:intergenic
GAAGGCGTTGGTAGTGGTCA+TGG 0.541151 6:+31338234 None:intergenic
GTGGAAGGAGTAGTGAGGGT+GGG 0.544821 6:+31338274 None:intergenic
GAGGGATTTCTGATTGACTA+GGG 0.547273 6:+31336448 None:intergenic
TGGTGTTTGAGATGGAGATG+GGG 0.548223 6:+31338765 None:intergenic
GGTTTGGATAATAGTGATTG+GGG 0.549958 6:+31338501 None:intergenic
AAACCATCAAAGTAAAATGG+TGG 0.550726 6:+31338688 None:intergenic
ATCACTAACACTAGTGCTCA+TGG 0.555133 6:-31338436 MsaT031642.1:CDS
ATAAGCTTTCAGAAGATTGG+TGG 0.556521 6:-31337740 MsaT031642.1:CDS
GAGGTGGCGGTGACAGTGGT+TGG 0.557422 6:+31338745 None:intergenic
AGGGGTGGAAGGAGTAGTGA+GGG 0.559730 6:+31338270 None:intergenic
GTAATTCTAGTAGGGGTGGA+AGG 0.560785 6:+31338259 None:intergenic
TCAAGTTGGTGGTGGTGCAG+GGG 0.561949 6:+31338161 None:intergenic
TGATTGATTGAGCTTTCTTG+GGG 0.561989 6:+31337991 None:intergenic
TAAAATCCTAGATCTGGTGT+TGG 0.562048 6:+31337955 None:intergenic
GCCAATGAGTAGCAATGCCC+AGG 0.566515 6:-31336313 MsaT031642.1:CDS
CAGGGTTATTGTCTTGGGCA+AGG 0.568974 6:+31338662 None:intergenic
AAAAGAGGCGGGGTTAGTAA+AGG 0.569673 6:+31338795 None:intergenic
GGGGCATGGAGAGAGGTGGT+GGG 0.569845 6:+31338574 None:intergenic
ATGTCGATCATCCAGAGAGC+AGG 0.571282 6:-31336485 MsaT031642.1:CDS
GACGTTATTGGTGATGGAGG+TGG 0.571488 6:+31338729 None:intergenic
TTTCAGAAGATTGGTGGTCG+TGG 0.572428 6:-31337734 MsaT031642.1:CDS
TGTACCAAATGTTATGTGGC+CGG 0.574015 6:+31338459 None:intergenic
AGCAATGCCCAGGTTTACTC+CGG 0.575866 6:-31336303 MsaT031642.1:CDS
GCAGGGGCTAATAGGTGCAG+TGG 0.576808 6:+31338177 None:intergenic
AGCTTTGTTGCTATTGACAA+AGG 0.577889 6:+31338822 None:intergenic
GGCGGTAATTCTAGTAGGGG+TGG 0.578698 6:+31338255 None:intergenic
TGAAATGGTAGGATATTGAC+TGG 0.578765 6:+31338111 None:intergenic
AACCATCAAAGTAAAATGGT+GGG 0.584704 6:+31338689 None:intergenic
AATGCATAATCTGAAATGGT+AGG 0.587445 6:+31338100 None:intergenic
TTAGCGGGTCCCCACATCCA+AGG 0.592475 6:-31337689 MsaT031642.1:intron
TGTGGGGTTAGCGAAGGCGT+TGG 0.595580 6:+31338222 None:intergenic
TGATTGTACCAAATGTTATG+TGG 0.596955 6:+31338455 None:intergenic
AAAGCTTATTTGTTCTCACA+TGG 0.598586 6:+31337754 None:intergenic
GACCTGAAATTTCCAACAGC+GGG 0.604025 6:+31336555 None:intergenic
AGTTGTTGTGGGGTTAGCGA+AGG 0.605077 6:+31338216 None:intergenic
GATGGAGGTGGCGGTGACAG+TGG 0.608901 6:+31338741 None:intergenic
CTTTGATGACTTGTTCTGGG+AGG 0.611922 6:+31336587 None:intergenic
TGATTCACCGGAGTAAACCT+GGG 0.612077 6:+31336296 None:intergenic
GGGGCTAATAGGTGCAGTGG+TGG 0.614826 6:+31338180 None:intergenic
GTTATTGGTGATGGAGGTGG+CGG 0.616856 6:+31338732 None:intergenic
GAAATAGAAAACCATCATTG+AGG 0.620026 6:+31336163 None:intergenic
TGATTTAATTTCTCTAGCAG+CGG 0.626290 6:+31336392 None:intergenic
GATAATGTTAGTCAAGTTGG+TGG 0.636625 6:+31338150 None:intergenic
AATGTTAGTCAAGTTGGTGG+TGG 0.642962 6:+31338153 None:intergenic
TATGACGTTATTGGTGATGG+AGG 0.643772 6:+31338726 None:intergenic
ATAGAAAACCATCATTGAGG+TGG 0.645904 6:+31336166 None:intergenic
CATGGCGGTAATTCTAGTAG+GGG 0.672579 6:+31338252 None:intergenic
AAACATGACTGATATGTCAG+AGG 0.673297 6:-31336208 MsaT031642.1:CDS
AATGTGGGTTGGGATAGTGG+TGG 0.673840 6:+31338540 None:intergenic
GATTGATTGAGCTTTCTTGG+GGG 0.688353 6:+31337992 None:intergenic
CGACCTGAAATTTCCAACAG+CGG 0.704425 6:+31336554 None:intergenic
GGCGTTGGTAGTGGTCATGG+CGG 0.717922 6:+31338237 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 31336158 31338872 31336158 ID=MsaG031642
Chr6 mRNA 31336158 31338872 31336158 ID=MsaT031642.1;Parent=MsaG031642
Chr6 exon 31336158 31336758 31336158 ID=MsaT031642.1.exon3;Parent=MsaT031642.1
Chr6 CDS 31336158 31336758 31336158 ID=cds.MsaT031642.1;Parent=MsaT031642.1
Chr6 exon 31337690 31337806 31337690 ID=MsaT031642.1.exon2;Parent=MsaT031642.1
Chr6 CDS 31337690 31337806 31337690 ID=cds.MsaT031642.1;Parent=MsaT031642.1
Chr6 exon 31337911 31338872 31337911 ID=MsaT031642.1.exon1;Parent=MsaT031642.1
Chr6 CDS 31337911 31338872 31337911 ID=cds.MsaT031642.1;Parent=MsaT031642.1
Gene Sequence

>MsaT031642.1

ATGGACATTGACGATTGGGATCTCTTTTCCTTTGTCAATAGCAACAAAGCTACAACCTTTACTAACCCCGCCTCTTTTTTTGAAACCCCATCTCCATCTCAAACACCAACCACTGTCACCGCCACCTCCATCACCAATAACGTCATATCTCCTCAAAATATCCCACCATTTTACTTTGATGGTTTTAGCCTTGCCCAAGACAATAACCCTGTTCCTTTTTCCCCACTTAAACAAAATGATTTCATAGATCTAGACAAATTGAAAATCAATTCCAATCCCACCACCTCTCTCCATGCCCCCATCCCAACCACCACCACTATCCCAACCCACATTACAACAATCACTTCATCCCCAATCACTATTATCCAAACCCCTTCAACAACCACTATTCCCGGCCACATAACATTTGGTACAATCACTAACACTAGTGCTCATGGTTTCAATAAAAAACCCACCTTTTTTGATTGTCCAACACTTATCGAACAACAAGAAATGCAATCAAATGGGCACGAGCTAAATAAATTTATACTCAACAAAAATCCTGTTAACCCCACCCCTACCAATAATTTCAATACTCCCACCCTCACTACTCCTTCCACCCCTACTAGAATTACCGCCATGACCACTACCAACGCCTTCGCTAACCCCACAACAACTTTTTTCACTCCGACCACCACTGCACCTATTAGCCCCTGCACCACCACCAACTTGACTAACATTATCACTAGTGTTCATGGAACCAGTCAATATCCTACCATTTCAGATTATGCATTACTCATCAAACAACAACAAATTCAACCAAACAATAACAATCAAGTGTTTGATCATGCATACAACTATTCATTTGTTCCACAACAACCCCCAAGAAAGCTCAATCAATCACAAATTCAATTACCCAACACCAGATCTAGGATTTTACCTAATTCAGATTCACAAAAAGCAAAACATAATTCAAGAATCAGAAAAATTAACAACAAGGTAACTTTGGAATGCCATGTGAGAACAAATAAGCTTTCAGAAGATTGGTGGTCGTGGACAAAATATGGACAAAAAAACATTAGCGGGTCCCCACATCCAAGGAGCTACTTTAAATGTAGCAGTTCTAAAGATTGTTCAGCAAAAAAACTAGTACAAAAAAGTGACACCAAAGAGGACACTTTTGTTGTGACGTTTGTTGGAGCACACAACCATGAAAAACCAGCAGGTAAATGGAACTCTATTGGTCTGACCTCCCAGAACAAGTCATCAAAGACTAGATTACCCGCTGTTGGAAATTTCAGGTCGTCACCAAACATTTCAAAGCTGGGTTCTCAAAATGTGACGATGATGCATGTCGATCATCCAGAGAGCAGGAATATTCAAGTTCTCCCTAGTCAATCAGAAATCCCTCATTCAGAAACTGAACTTAGAGAGACCCATTTACCCGCTGCTAGAGAAATTAAATCATCTCCTAATGTTGAAAACTTGTTTTCATATAATGTGACGATGGTACAGTTTGATGAGCCAATGAGTAGCAATGCCCAGGTTTACTCCGGTGAATCACAAATCCTGCCTTTAGGAAACAAACTTATTGGAAACTCTGATGACGATGAAGAAATTATGATACCAAACATGACTGATATGTCAGAGGATTTCTTATTGGATTTTGACCACCTCAATGATGGTTTTCTATTTCCTTAG
Protein sequence

>MsaT031642.1

MDIDDWDLFSFVNSNKATTFTNPASFFETPSPSQTPTTVTATSITNNVISPQNIPPFYFDGFSLAQDNNPVPFSPLKQNDFIDLDKLKINSNPTTSLHAPIPTTTTIPTHITTITSSPITIIQTPSTTTIPGHITFGTITNTSAHGFNKKPTFFDCPTLIEQQEMQSNGHELNKFILNKNPVNPTPTNNFNTPTLTTPSTPTRITAMTTTNAFANPTTTFFTPTTTAPISPCTTTNLTNIITSVHGTSQYPTISDYALLIKQQQIQPNNNNQVFDHAYNYSFVPQQPPRKLNQSQIQLPNTRSRILPNSDSQKAKHNSRIRKINNKVTLECHVRTNKLSEDWWSWTKYGQKNISGSPHPRSYFKCSSSKDCSAKKLVQKSDTKEDTFVVTFVGAHNHEKPAGKWNSIGLTSQNKSSKTRLPAVGNFRSSPNISKLGSQNVTMMHVDHPESRNIQVLPSQSEIPHSETELRETHLPAAREIKSSPNVENLFSYNVTMVQFDEPMSSNAQVYSGESQILPLGNKLIGNSDDDEEIMIPNMTDMSEDFLLDFDHLNDGFLFP*