AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG031821


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG031821 MtrunA17_Chr2g0308841 67.826 115 35 1 252 366 153 265 3.29e-49 166
MsaG031821 MtrunA17_Chr1g0203051 33.898 295 164 6 6 278 5 290 2.68e-48 172
MsaG031821 MtrunA17_Chr1g0171741 39.752 161 95 2 53 212 103 262 8.57e-33 123
MsaG031821 MtrunA17_Chr8g0389811 43.307 127 72 0 67 193 69 195 1.25e-31 118
MsaG031821 MtrunA17_Chr2g0287651 35.870 184 118 0 82 265 75 258 2.26e-31 125
MsaG031821 MtrunA17_Chr2g0331371 41.401 157 90 1 32 186 3 159 4.05e-29 112
MsaG031821 MtrunA17_Chr6g0473421 45.263 95 52 0 64 158 37 131 4.66e-24 96.7
MsaG031821 MtrunA17_Chr1g0172931 36.508 126 80 0 39 164 33 158 5.01e-20 85.9
MsaG031821 MtrunA17_Chr5g0448761 30.636 173 118 2 84 254 62 234 1.55e-15 78.2
MsaG031821 MtrunA17_Chr1g0202341 27.746 173 120 3 83 254 52 220 2.46e-14 74.3
MsaG031821 MtrunA17_Chr2g0276841 36.232 138 83 4 88 221 76 212 6.35e-14 73.2
MsaG031821 MtrunA17_Chr2g0290391 31.548 168 109 4 88 251 57 222 4.97e-13 70.5
MsaG031821 MtrunA17_Chr6g0458391 35.714 126 70 4 87 208 96 214 9.06e-13 69.7
MsaG031821 MtrunA17_Chr1g0198761 29.775 178 102 7 88 249 53 223 2.19e-12 68.6
MsaG031821 MtrunA17_Chr6g0451441 26.761 213 140 6 45 250 44 247 4.26e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG031821 AT5G28530.5 34.568 162 102 3 88 246 61 221 4.02e-19 89.4
MsaG031821 AT5G28530.4 34.568 162 102 3 88 246 61 221 4.23e-19 89.4
MsaG031821 AT5G28530.3 34.568 162 102 3 88 246 61 221 5.12e-19 89.0
MsaG031821 AT5G28530.2 34.568 162 102 3 88 246 61 221 5.12e-19 89.0
MsaG031821 AT5G28530.1 34.568 162 102 3 88 246 61 221 5.63e-19 89.0
MsaG031821 AT4G38180.1 30.508 236 148 8 38 259 17 250 1.54e-15 78.6
MsaG031821 AT1G10240.2 34.756 164 97 6 88 244 54 214 4.49e-15 77.0
MsaG031821 AT1G10240.1 34.756 164 97 6 88 244 54 214 4.49e-15 77.0

Find 80 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGGCTTTGGCAGATGCTTC+TGG 0.082781 6:-36698512 MsaT031821.1:CDS
TTTGTTCATGTCAAGGTTAC+AGG 0.251328 6:-36698796 MsaT031821.1:CDS
GGGCTTTGGCAGATGCTTCT+GGG 0.266190 6:-36698511 MsaT031821.1:CDS
CTGGGCAAAACCAAACATTT+TGG 0.278132 6:+36697311 None:intergenic
GATCACAATCATGCTATGTT+AGG 0.329562 6:-36698641 MsaT031821.1:CDS
TGATGATCTTCCAAAATGTT+TGG 0.355568 6:-36697321 MsaT031821.1:CDS
TCCGATTATGATAAAAGATA+TGG 0.367150 6:-36698999 MsaT031821.1:CDS
AACTGAAAATCCTGACGTTC+TGG 0.369216 6:+36698874 None:intergenic
AAACAATGATTTACGTTGCT+TGG 0.383660 6:+36698439 None:intergenic
CACGAGCATTTCAAAACAAC+TGG 0.401503 6:+36697410 None:intergenic
GGAAGTTCAAATTCAGTTGA+AGG 0.407056 6:-36699049 MsaT031821.1:CDS
TCTTGGTTTGCCAGAACGTC+AGG 0.409357 6:-36698884 MsaT031821.1:CDS
TCAAGGTTACAGGGAGGATA+GGG 0.412309 6:-36698786 MsaT031821.1:CDS
GTACCAAATGTATGGGGCTT+TGG 0.414713 6:-36698525 MsaT031821.1:CDS
CTGCCAAAGCCCCATACATT+TGG 0.417656 6:+36698522 None:intergenic
AAATTCAGTTGAAGGCTTAG+AGG 0.428492 6:-36699041 MsaT031821.1:CDS
GAAATGCTCGTGTCTTAGAA+TGG 0.443162 6:-36697399 MsaT031821.1:CDS
TTGGAAGATCATCAAAATCA+AGG 0.448932 6:+36697330 None:intergenic
TTTAGCAAACTTGGACCACC+TGG 0.451664 6:+36697292 None:intergenic
TGCAAAATTTCGAGTCCACT+TGG 0.456820 6:-36698705 MsaT031821.1:CDS
ATAAGACCGTACCAAATGTA+TGG 0.463653 6:-36698533 MsaT031821.1:CDS
GGCTTTGGCAGATGCTTCTG+GGG 0.464116 6:-36698510 MsaT031821.1:CDS
TTTGAAGGACATGCTGAGCC+AGG 0.465178 6:-36696009 MsaT031821.1:intron
CCATCAGTCGCATCAATGTT+GGG 0.475672 6:-36696893 MsaT031821.1:CDS
ACCTTTGTTTGTTCATGTCA+AGG 0.486075 6:-36698803 MsaT031821.1:CDS
GACGGTACACGTCTCAATCC+TGG 0.486316 6:+36695991 None:intergenic
CAAAATACCGTGGTGATGGT+AGG 0.487602 6:-36697460 MsaT031821.1:CDS
ACCGTGGTGATGGTAGGGTT+TGG 0.493348 6:-36697454 MsaT031821.1:CDS
ATGAACAAACAAAGGTCTGC+TGG 0.497463 6:+36698810 None:intergenic
TCCATCAGTCGCATCAATGT+TGG 0.500041 6:-36696894 MsaT031821.1:CDS
TGTTGTACGCATCCTCGTCT+TGG 0.504705 6:+36696832 None:intergenic
ATTTACGTTGCTTGGCAACT+TGG 0.506669 6:+36698447 None:intergenic
GTCAAGGTTACAGGGAGGAT+AGG 0.518923 6:-36698787 MsaT031821.1:CDS
GCTTTGGCAGATGCTTCTGG+GGG 0.524995 6:-36698509 MsaT031821.1:CDS
AAAATACCGTGGTGATGGTA+GGG 0.526246 6:-36697459 MsaT031821.1:CDS
TTCACTTACATTGTAACCCT+CGG 0.528797 6:+36696809 None:intergenic
TGTCTTAGAATGGAATCGCT+TGG 0.529768 6:-36697389 MsaT031821.1:CDS
CAAAGCAATGGAGATGTCAA+TGG 0.531347 6:-36699070 MsaT031821.1:CDS
CCCAACATTGATGCGACTGA+TGG 0.535314 6:+36696893 None:intergenic
TAAGACCGTACCAAATGTAT+GGG 0.537974 6:-36698532 MsaT031821.1:CDS
ACACCATCGCGCTCTAACAA+AGG 0.543044 6:+36699235 None:intergenic
ATTGAGCGTAGTCGAAAAGC+TGG 0.551197 6:-36698557 MsaT031821.1:CDS
CACAATCATGCTATGTTAGG+AGG 0.553509 6:-36698638 MsaT031821.1:CDS
TTGTTCATGTCAAGGTTACA+GGG 0.556666 6:-36698795 MsaT031821.1:CDS
TGATATAATGAAGATTGAGC+TGG 0.557270 6:-36698975 MsaT031821.1:CDS
GTTTCAAAATACCGTGGTGA+TGG 0.561793 6:-36697464 MsaT031821.1:CDS
AGAAATGGACGGACGTTCAG+AGG 0.566572 6:-36699266 None:intergenic
TCGTAAGGGGCAAGTGGTGA+GGG 0.567327 6:-36698852 MsaT031821.1:CDS
ACGCTCAATTTCAGCTATGT+CGG 0.568884 6:+36698571 None:intergenic
TTCGTAAGGGGCAAGTGGTG+AGG 0.571006 6:-36698853 MsaT031821.1:CDS
ATCCCTTCGTCAGTTGACAT+TGG 0.575813 6:+36696858 None:intergenic
TTCAGTTCGTAAGGGGCAAG+TGG 0.578338 6:-36698858 MsaT031821.1:CDS
AGAAAGGCAAGAAATGTCCA+TGG 0.584202 6:-36697501 MsaT031821.1:CDS
GAAAGGCAAGAAATGTCCAT+GGG 0.588349 6:-36697500 MsaT031821.1:CDS
CAAGTGGTGAGGGATCGAAA+TGG 0.589736 6:-36698842 MsaT031821.1:CDS
ACATTGATGCGACTGATGGA+AGG 0.591550 6:+36696897 None:intergenic
AAACCAAGAGTAAAACATGT+AGG 0.598768 6:+36698898 None:intergenic
AACCGTGAAAATAAACCCCA+TGG 0.607032 6:+36697484 None:intergenic
CAAGGTTACAGGGAGGATAG+GGG 0.609406 6:-36698785 MsaT031821.1:CDS
AAGGTTACAGGGAGGATAGG+GGG 0.615842 6:-36698784 MsaT031821.1:CDS
AAAGCCCCATACATTTGGTA+CGG 0.620279 6:+36698527 None:intergenic
CACCAATGTCAACTGACGAA+GGG 0.623535 6:-36696860 MsaT031821.1:CDS
ACAAATTGCGAACAAAGCAA+TGG 0.627176 6:-36699082 MsaT031821.1:CDS
ACACCAATGTCAACTGACGA+AGG 0.627495 6:-36696861 MsaT031821.1:CDS
CGAAGGGATTCTCCAAGACG+AGG 0.638814 6:-36696844 MsaT031821.1:CDS
TTCACGGTTTCAAAATACCG+TGG 0.639319 6:-36697470 MsaT031821.1:CDS
GCCTCGACAAAATATCCAAG+TGG 0.642964 6:+36698690 None:intergenic
ACCTTGACATGAACAAACAA+AGG 0.644270 6:+36698802 None:intergenic
TTGGTATTCAGAGAAATGGA+CGG 0.649153 6:-36699277 None:intergenic
AATCCTTTGTTAGAGCGCGA+TGG 0.654133 6:-36699238 MsaT031821.1:CDS
CAAGGTACAGAAGCACAGCA+AGG 0.654464 6:+36697348 None:intergenic
TTAGCAAACTTGGACCACCT+GGG 0.656483 6:+36697293 None:intergenic
ACAGCAAGGATATGATCGCA+CGG 0.663089 6:+36697362 None:intergenic
GCCAAACCCTACCATCACCA+CGG 0.683425 6:+36697453 None:intergenic
GAGGATGCGTACAACACCGA+GGG 0.685934 6:-36696825 MsaT031821.1:CDS
ATGTTAGGAGGTACTCTGTG+TGG 0.688101 6:-36698626 MsaT031821.1:CDS
AAGACCGTACCAAATGTATG+GGG 0.707217 6:-36698531 MsaT031821.1:CDS
TTCATGTCAAGGTTACAGGG+AGG 0.709283 6:-36698792 MsaT031821.1:CDS
CGAGGATGCGTACAACACCG+AGG 0.735213 6:-36696826 MsaT031821.1:CDS
AAAGGCAAGAAATGTCCATG+GGG 0.742239 6:-36697499 MsaT031821.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 36696004 36699284 36696004 ID=MsaG031821
Chr6 mRNA 36696004 36699284 36696004 ID=MsaT031821.1;Parent=MsaG031821
Chr6 exon 36696004 36696024 36696004 ID=MsaT031821.1.exon5;Parent=MsaT031821.1
Chr6 CDS 36696004 36696024 36696004 ID=cds.MsaT031821.1;Parent=MsaT031821.1
Chr6 exon 36696818 36696929 36696818 ID=MsaT031821.1.exon4;Parent=MsaT031821.1
Chr6 CDS 36696818 36696929 36696818 ID=cds.MsaT031821.1;Parent=MsaT031821.1
Chr6 exon 36697302 36697521 36697302 ID=MsaT031821.1.exon3;Parent=MsaT031821.1
Chr6 CDS 36697302 36697521 36697302 ID=cds.MsaT031821.1;Parent=MsaT031821.1
Chr6 exon 36698453 36699137 36698453 ID=MsaT031821.1.exon2;Parent=MsaT031821.1
Chr6 CDS 36698453 36699137 36698453 ID=cds.MsaT031821.1;Parent=MsaT031821.1
Chr6 exon 36699222 36699284 36699222 ID=MsaT031821.1.exon1;Parent=MsaT031821.1
Chr6 CDS 36699222 36699284 36699222 ID=cds.MsaT031821.1;Parent=MsaT031821.1
Gene Sequence

>MsaT031821.1

ATGGACGGACGTTCAGAGGCTGTGAATCCTTTGTTAGAGCGCGATGGTGTGAGAATTTCAAGAGATTTTGAAGCTGAAAGTGGAGGTAATTACAACACAAATTGCGAACAAAGCAATGGAGATGTCAATGGAAGTTCAAATTCAGTTGAAGGCTTAGAGGATGAAACTAATGAAGTCAATCCGATTATGATAAAAGATATGGTTGATATAATGAAGATTGAGCTGGAAAACTTTTGTGTGGCTGATGTGGAAAGATATGAATTTGTTGCTTTAGATGTTGCCTACATGTTTTACTCTTGGTTTGCCAGAACGTCAGGATTTTCAGTTCGTAAGGGGCAAGTGGTGAGGGATCGAAATGGTGAAGTCGTCCAGCAGACCTTTGTTTGTTCATGTCAAGGTTACAGGGAGGATAGGGGGTTAACAATGGAAAATCGAAAGCGTGAGCCAAAAAATGAAACAAGATGTGGGTGTAATGCAAAATTTCGAGTCCACTTGGATATTTTGTCGAGGCATTGGTATGTTACTGTATTTGAATTTGATCACAATCATGCTATGTTAGGAGGTACTCTGTGTGGAATGTTGCCAGCGTATAGAAAAATGAGTGTAGCCGACATAGCTGAAATTGAGCGTAGTCGAAAAGCTGGTATAAGACCGTACCAAATGTATGGGGCTTTGGCAGATGCTTCTGGGGGTTTTCATAAGGTAGGATTTGTTAAAAAAGATTTGTACAACCAAGTTGCCAAGCAACAAAGGCAAGAAATGTCCATGGGGTTTATTTTCACGGTTTCAAAATACCGTGGTGATGGTAGGGTTTGGCATGTGACATTTTGTGAAGAACCAGTTGTTTTGAAATGCTCGTGTCTTAGAATGGAATCGCTTGGTTTGCCGTGCGATCATATCCTTGCTGTGCTTCTGTACCTTGATTTTGATGATCTTCCAAAATGTTTGGTTTTGCCCAGGTGGTCCAAACGTTGTTGTCCTTCCATCAGTCGCATCAATGTTGGGCAGACACAAACACCAATGTCAACTGACGAAGGGATTCTCCAAGACGAGGATGCGTACAACACCGAGGGTTACAATGACATGCTGAGCCAGGATTGA

Protein sequence

>MsaT031821.1

MDGRSEAVNPLLERDGVRISRDFEAESGGNYNTNCEQSNGDVNGSSNSVEGLEDETNEVNPIMIKDMVDIMKIELENFCVADVERYEFVALDVAYMFYSWFARTSGFSVRKGQVVRDRNGEVVQQTFVCSCQGYREDRGLTMENRKREPKNETRCGCNAKFRVHLDILSRHWYVTVFEFDHNHAMLGGTLCGMLPAYRKMSVADIAEIERSRKAGIRPYQMYGALADASGGFHKVGFVKKDLYNQVAKQQRQEMSMGFIFTVSKYRGDGRVWHVTFCEEPVVLKCSCLRMESLGLPCDHILAVLLYLDFDDLPKCLVLPRWSKRCCPSISRINVGQTQTPMSTDEGILQDEDAYNTEGYNDMLSQD*