AlfalfaGEDB | MsaG031824

M. sativa ssp. caerulea / MsaG031824

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004672 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010941 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0031347 GO:0031348 GO:0033612 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0060548 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564
PFAM: PF08263.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG031824	XP_003608312.3	81.198	601	65	5	1	560	34	627	0.0	973

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG031824	sp\|Q9ASS4\|Y5838_ARATH	33.597	631	300	13	7	558	27	617	9.26e-105	331

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG031824	tr\|A0A396IAH3\|A0A396IAH3_MEDTR	81.198	601	65	5	1	560	41	634	0.0	973

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG031824	MtrunA17_Chr4g0049181	81.198	601	65	5	1	560	41	634	0.0	973
MsaG031824	MtrunA17_Chr4g0049201	65.128	390	89	4	203	552	50	432	6.92e-159	460
MsaG031824	MtrunA17_Chr4g0049161	74.441	313	36	3	289	560	4	313	7.72e-159	456
MsaG031824	MtrunA17_Chr8g0337671	42.429	601	241	13	8	539	30	594	1.11e-138	415
MsaG031824	MtrunA17_Chr7g0249841	40.878	592	240	15	8	529	29	580	2.47e-131	396
MsaG031824	MtrunA17_Chr4g0049171	60.887	248	90	4	23	267	35	278	2.22e-91	281
MsaG031824	MtrunA17_Chr6g0452221	30.116	601	329	13	2	541	19	589	2.21e-76	253
MsaG031824	MtrunA17_Chr5g0423761	35.714	406	192	14	204	555	7	397	8.64e-60	203
MsaG031824	MtrunA17_Chr4g0049211	41.176	272	100	7	309	529	9	271	1.28e-51	178
MsaG031824	MtrunA17_Chr3g0129391	29.815	540	266	19	82	540	651	1158	1.46e-42	164

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG031824	AT5G48380.1	33.597	631	300	13	7	558	27	617	9.41e-106	331
MsaG031824	AT3G28450.1	32.040	593	314	15	7	541	29	590	4.83e-81	266
MsaG031824	AT1G69990.1	30.085	585	324	13	1	529	11	566	5.56e-75	249
MsaG031824	AT1G27190.1	31.516	587	306	14	8	529	24	579	1.21e-72	243
MsaG031824	AT5G07280.1	28.625	538	294	16	59	529	678	1192	1.49e-46	176
MsaG031824	AT5G07280.1	41.346	104	60	1	69	172	592	694	2.77e-13	73.6
MsaG031824	AT5G07280.1	37.500	128	69	1	55	171	518	645	6.29e-13	72.4
MsaG031824	AT5G07280.1	38.835	103	62	1	69	171	460	561	1.76e-12	70.9
MsaG031824	AT5G07280.1	44.706	85	46	1	87	171	586	669	4.34e-12	69.7
MsaG031824	AT5G07280.1	35.115	131	63	1	62	170	263	393	1.68e-11	67.8

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Find 121 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATAGTTCCCATGCTT+GGG	0.428215	6:+36850982	MsaT031824.1:CDS
AAAATGAGCAATGTTAGGAA+GGG	0.616497	6:+36854493	MsaT031824.1:CDS
AAATCTCAAACTATCTAACA+TGG	0.543158	6:+36849942	MsaT031824.1:CDS
AAATTTGGCCTCTCCAAAAT+TGG	0.218462	6:-36851249	None:intergenic
AACATTGGAGGTGAGAAGGG+AGG	0.560798	6:+36851301	MsaT031824.1:CDS
AACCTGTTCCTTTCCTTCAA+TGG	0.335560	6:-36851009	None:intergenic
AACGACTTGGCACGCTTAAC+AGG	0.293829	6:+36850205	MsaT031824.1:CDS
AACTTCAACAACAAAACTGA+AGG	0.560611	6:+36849862	MsaT031824.1:CDS
AAGAAGGATGTCTACGACTT+TGG	0.316828	6:+36854247	MsaT031824.1:CDS
AAGACGGGAATGATGTACCA+AGG	0.698419	6:+36850852	MsaT031824.1:CDS

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	36849763	36854603	36849763	ID=MsaG031824
Chr6	mRNA	36849763	36854603	36849763	ID=MsaT031824.1;Parent=MsaG031824
Chr6	exon	36849763	36850629	36849763	ID=MsaT031824.1.exon1;Parent=MsaT031824.1
Chr6	CDS	36849763	36850629	36849763	ID=cds.MsaT031824.1;Parent=MsaT031824.1
Chr6	exon	36850753	36851089	36850753	ID=MsaT031824.1.exon2;Parent=MsaT031824.1
Chr6	CDS	36850753	36851089	36850753	ID=cds.MsaT031824.1;Parent=MsaT031824.1
Chr6	exon	36851189	36851325	36851189	ID=MsaT031824.1.exon3;Parent=MsaT031824.1
Chr6	CDS	36851189	36851325	36851189	ID=cds.MsaT031824.1;Parent=MsaT031824.1
Chr6	exon	36854238	36854436	36854238	ID=MsaT031824.1.exon4;Parent=MsaT031824.1
Chr6	CDS	36854238	36854436	36854238	ID=cds.MsaT031824.1;Parent=MsaT031824.1
Chr6	exon	36854461	36854603	36854461	ID=MsaT031824.1.exon5;Parent=MsaT031824.1
Chr6	CDS	36854461	36854603	36854461	ID=cds.MsaT031824.1;Parent=MsaT031824.1

Gene Sequence

>MsaT031824.1

ATGTTAATGACTAGTCAGAGTACCGAGACTGATATCTTCTGTTTAAAATCCATTAAGAATTCCTTGCAAGACCCAAATAATTACTTAACCTCAAGCTGGAACTTCAACAACAAAACTGAAGGTTTCATATGTAGATTTAAGGGTGTTGAATGTTGGCATCCAGATGAGAATAAAGTACTAAATCTCAAACTATCTAACATGGGGTTGAAGGGTAAGTTTCCACGTGGACTTGAGAATTGCTTTTCGTTGACGGGCTTAGACCTTTCAGTCAACGAGCTCTCAGGATCCATCCCATCTGATATATCAACAATACTCAAATTTGTTACAACTATTGATCTATCTACTAACAAATTCACTGGTGAAATTCCGACAACTTTGGCAAATTGCACTTACCTGAATAATCTTATACTTAACAACAATATGTTAAGTGGTGAAATTCCAAAACGACTTGGCACGCTTAACAGGCTTAAAGTGATTGATTTTTCCAACAATCAATTGCATGGGCAAGTGCCAGTGTTTAATGAAGGAGCAGCTGTTAACTATGCTAACAATAGAAGACTTTGTGGAGCGCCTTTGAGACCTTGCCACTTGACACATGATAACGGCGATTTTCATCTATCATTCAAAAGTGGTCTTATTGTTGGTTACGTTTTTTCACTCATTTCTTCTGTTACACTCATTTGTATCTTCTACTCCAAATGTGCACATTGGGTGCATCAATCGAAAAAGAAGAAAAACAATCACCTAAACAAAGCCATAGAATTGGGAAAGCATATTATCTCCATCACCAGCAGGAGAACACAGATGGTAGTTAATCAGATGCACCAATTGTTAAATTCGTGGATTGTACCCAAAGAAACCAAAGAGATCTCTATACTAATGGAGAGACTAACTTCAACAATATGGTTGGAAGAACTGCGTGACACAACCGATTGTTTTGCTGTGGACAATGCCATTGGGGTAGGAAAGACGGGAATGATGTACCAAGGATTTCTGCCTAATGATCAATTACTAGCTGTTAAGAGACTATTTGACTCTCATTTATTTAAAAGGCATATCCTTTTGGAAACAATGATTATGGGTAGGTACAGACACAAAAATATAGTTCCCATGCTTGGGTTCTCCATTGAAGGAAAGGAACAGGTTTTGGCATATGCATACATGTCAAATGGGAGGCTTTCCAAATGGCTGCATCCTTTGGAAATGCATTTTAATATATGTTCAGAATGCATATTGCTAGATGAGAATTTTGAACCCAAAATATCCAATTTTGGAGAGGCCAAATTTATGAATCCCAACATTGAAGATCATTTAGGAACATTGGAGGTGAGAAGGGAGGTGAATGATGGGAAGAAGGATGTCTACGACTTTGGAAGTGTGCTTTTTGAATTGATGACAGGGAAAACATATAATGAATTGTCGCATTCATCTGATACTACTAATCTATGTGGTAACCCTTTGAGTTTTTATAATGTCATTGATAAATCTTTGACCGGGGAAGGACTTGAAAATGAAGTTTGTACTCTCATCAAGATTGCCTACCAAAGGCCAACAATGCTTGAAGTTTACAACAAAATGAGCAATGTTAGGAAGGGACAAAATGGCTCTGTTAATGATTCTGACAGATTAAGGGGATTAGAATATGCTTCTTCAATTACTATAGACGAAATTATTGCGTTTTAA

Protein sequence

>MsaT031824.1

MLMTSQSTETDIFCLKSIKNSLQDPNNYLTSSWNFNNKTEGFICRFKGVECWHPDENKVLNLKLSNMGLKGKFPRGLENCFSLTGLDLSVNELSGSIPSDISTILKFVTTIDLSTNKFTGEIPTTLANCTYLNNLILNNNMLSGEIPKRLGTLNRLKVIDFSNNQLHGQVPVFNEGAAVNYANNRRLCGAPLRPCHLTHDNGDFHLSFKSGLIVGYVFSLISSVTLICIFYSKCAHWVHQSKKKKNNHLNKAIELGKHIISITSRRTQMVVNQMHQLLNSWIVPKETKEISILMERLTSTIWLEELRDTTDCFAVDNAIGVGKTGMMYQGFLPNDQLLAVKRLFDSHLFKRHILLETMIMGRYRHKNIVPMLGFSIEGKEQVLAYAYMSNGRLSKWLHPLEMHFNICSECILLDENFEPKISNFGEAKFMNPNIEDHLGTLEVRREVNDGKKDVYDFGSVLFELMTGKTYNELSHSSDTTNLCGNPLSFYNVIDKSLTGEGLENEVCTLIKIAYQRPTMLEVYNKMSNVRKGQNGSVNDSDRLRGLEYASSITIDEIIAF*