AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG031933


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG031933 MtrunA17_Chr3g0105741 66.667 75 21 3 22 95 9 80 2.37e-25 98.2
MsaG031933 MtrunA17_Chr3g0105741 47.778 90 44 3 7 95 89 176 1.27e-15 72.4
MsaG031933 MtrunA17_Chr2g0316551 41.667 96 56 0 107 202 207 302 1.37e-17 80.1
MsaG031933 MtrunA17_Chr4g0040891 43.158 95 54 0 108 202 194 288 2.31e-17 79.3
MsaG031933 MtrunA17_Chr2g0325131 43.158 95 54 0 108 202 191 285 3.99e-17 79.0
MsaG031933 MtrunA17_Chr5g0432701 28.188 149 99 1 82 222 125 273 2.17e-11 62.4
MsaG031933 MtrunA17_Chr3g0107491 38.298 94 58 0 107 200 176 269 2.99e-11 62.0
MsaG031933 MtrunA17_Chr4g0036071 35.922 103 66 0 108 210 360 462 6.36e-11 61.2
MsaG031933 MtrunA17_Chr6g0463571 36.792 106 67 0 107 212 150 255 9.07e-11 60.5
MsaG031933 MtrunA17_Chr2g0329551 36.458 96 61 0 107 202 220 315 9.51e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG031933 AT2G42380.2 40.179 112 67 0 91 202 169 280 1.52e-18 82.8
MsaG031933 AT3G58120.1 40.708 113 66 1 90 202 185 296 2.46e-18 82.4
MsaG031933 AT2G42380.5 41.667 96 56 0 91 186 169 264 4.64e-16 75.5
MsaG031933 AT3G58120.2 42.268 97 55 1 90 186 185 280 7.11e-16 75.1
MsaG031933 AT2G42380.1 35.714 112 61 1 91 202 169 269 1.31e-13 68.9
MsaG031933 AT5G07160.1 40.860 93 55 0 108 200 48 140 5.67e-12 62.0
MsaG031933 AT1G43700.1 35.593 118 69 1 83 200 176 286 1.09e-11 63.5
MsaG031933 AT1G06850.1 33.333 120 80 0 107 226 147 266 3.65e-11 62.0
MsaG031933 AT2G40620.1 38.298 94 58 0 107 200 147 240 4.22e-11 62.0

Find 39 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTATTTCAAGTCTTAAT+TGG 0.226858 6:+40111748 None:intergenic
GCTCGATTATATTGCTGATT+TGG 0.235809 6:-40112406 MsaT031933.1:CDS
GCATGACCAAGATTTGATTT+TGG 0.261059 6:+40113051 None:intergenic
TTTGTACCAGTCATTCTCTT+TGG 0.270280 6:+40113264 None:intergenic
GTATACCGTTTGAGCCTCTT+TGG 0.278573 6:+40112952 None:intergenic
TTGTACCAGTCATTCTCTTT+GGG 0.334410 6:+40113265 None:intergenic
ATAAGAGAGCTGAGGTGAAT+AGG 0.385871 6:-40111594 MsaT031933.1:CDS
TTCGATCATGGCAACCCTCT+TGG 0.403712 6:-40113161 MsaT031933.1:CDS
AGTGTACCAAAATCAAATCT+TGG 0.459311 6:-40113057 MsaT031933.1:CDS
CGTCATCAACATTTCGATCA+TGG 0.470936 6:-40113173 MsaT031933.1:CDS
GCTGAGAAGAACCACATCAT+TGG 0.483237 6:-40113030 MsaT031933.1:CDS
TATTGCTGATTTGGAGCGGA+TGG 0.492832 6:-40112397 MsaT031933.1:CDS
GAGCTGAGGTGAATAGGCTA+AGG 0.500065 6:-40111588 MsaT031933.1:CDS
TGGATCCAAAGAGGCTCAAA+CGG 0.511736 6:-40112957 MsaT031933.1:intron
AAACAAGAGGCGAGTAGCAG+TGG 0.525757 6:-40113120 MsaT031933.1:CDS
TCTTAATTGGTGATGTTCCA+TGG 0.529196 6:+40111761 None:intergenic
GGCATCCCAAAGAGAATGAC+TGG 0.538746 6:-40113270 MsaT031933.1:CDS
GTGCAGAAATTCGATTTGCT+AGG 0.542578 6:+40112448 None:intergenic
AACATGTTGCACACAGAAGA+GGG 0.550817 6:-40113243 MsaT031933.1:CDS
AGACTTCAACTTGTGGGCGA+AGG 0.559195 6:+40111808 None:intergenic
GATTGAGAATAAGAGAGCTG+AGG 0.559603 6:-40111602 MsaT031933.1:CDS
GCATCTGCATGTGTTGGTGT+TGG 0.564639 6:-40113000 MsaT031933.1:CDS
GAGAAGAACCACATCATTGG+CGG 0.593377 6:-40113027 MsaT031933.1:CDS
ATGCACTACCGCCAATGATG+TGG 0.601403 6:+40113019 None:intergenic
TTGCTGAAGCACAAAACAAG+AGG 0.602002 6:-40111546 MsaT031933.1:intron
AAACATGTTGCACACAGAAG+AGG 0.606246 6:-40113244 MsaT031933.1:CDS
GGTAGTGCATCTGCATGTGT+TGG 0.606657 6:-40113006 MsaT031933.1:CDS
TCAGCAGTGGTTGTTGACCA+TGG 0.606900 6:-40111778 MsaT031933.1:CDS
AGCTGAGGTGAATAGGCTAA+GGG 0.613143 6:-40111587 MsaT031933.1:CDS
ATGGTTAGACCCTCTTCACA+TGG 0.614802 6:-40113198 MsaT031933.1:CDS
ATTATATTGCTGATTTGGAG+CGG 0.619255 6:-40112401 MsaT031933.1:CDS
AAAGAAAGGATACTTCAAGA+AGG 0.624481 6:-40111717 MsaT031933.1:intron
AGGTCAATGCTCTTCCAAGA+GGG 0.634364 6:+40113147 None:intergenic
TACTCGCCTCTTGTTTACCG+AGG 0.637734 6:+40113127 None:intergenic
CATTGACCTCGGTAAACAAG+AGG 0.644199 6:-40113133 MsaT031933.1:CDS
GATGACGATCCATGTGAAGA+GGG 0.656652 6:+40113189 None:intergenic
GAGGTCAATGCTCTTCCAAG+AGG 0.660030 6:+40113146 None:intergenic
TGATGACGATCCATGTGAAG+AGG 0.681158 6:+40113188 None:intergenic
TCTTGGAAGAGCATTGACCT+CGG 0.690835 6:-40113144 MsaT031933.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 40111413 40113298 40111413 ID=MsaG031933
Chr6 mRNA 40111413 40113298 40111413 ID=MsaT031933.1;Parent=MsaG031933
Chr6 exon 40111413 40111452 40111413 ID=MsaT031933.1.exon5;Parent=MsaT031933.1
Chr6 CDS 40111413 40111452 40111413 ID=cds.MsaT031933.1;Parent=MsaT031933.1
Chr6 exon 40111547 40111628 40111547 ID=MsaT031933.1.exon4;Parent=MsaT031933.1
Chr6 CDS 40111547 40111628 40111547 ID=cds.MsaT031933.1;Parent=MsaT031933.1
Chr6 exon 40111718 40111844 40111718 ID=MsaT031933.1.exon3;Parent=MsaT031933.1
Chr6 CDS 40111718 40111844 40111718 ID=cds.MsaT031933.1;Parent=MsaT031933.1
Chr6 exon 40112383 40112473 40112383 ID=MsaT031933.1.exon2;Parent=MsaT031933.1
Chr6 CDS 40112383 40112473 40112383 ID=cds.MsaT031933.1;Parent=MsaT031933.1
Chr6 exon 40112958 40113298 40112958 ID=MsaT031933.1.exon1;Parent=MsaT031933.1
Chr6 CDS 40112958 40113298 40112958 ID=cds.MsaT031933.1;Parent=MsaT031933.1
Gene Sequence

>MsaT031933.1

ATGAAGGGCATCCCAAAGAGAATGACTGGTACAAACATGTTGCACACAGAAGAGGGACCTGAGCCTAAAAAATCAAACATGGTTAGACCCTCTTCACATGGATCGTCATCAACATTTCGATCATGGCAACCCTCTTGGAAGAGCATTGACCTCGGTAAACAAGAGGCGAGTAGCAGTGGAGTCAAAGCATCTCCACAAGACAAAAAAACTAATAACATTAGTGTACCAAAATCAAATCTTGGTCATGCTGAGAAGAACCACATCATTGGCGGTAGTGCATCTGCATGTGTTGGTGTTGGTGATCAAGCAACACAAAAACTGGATCCAAAGAGGCTCAAACGGATCCTAGCAAATCGAATTTCTGCACAAAAATCGAGATTGAAAAAGCTCGATTATATTGCTGATTTGGAGCGGATGGAAAAATATTTGCAGGATGAGTTATCTTTCCTTCGCCCACAAGTTGAAGTCTACAAAAATCAGCAGTGGTTGTTGACCATGGAACATCACCAATTAAGACTTGAAATAGCTGTACGAGAAAAAGAAAGGATACTTCAAGAAGTGGAGATTGAGAATAAGAGAGCTGAGGTGAATAGGCTAAGGGAGCTTCAGAAGAAACTTGTTGCTGAAGCACAAAACAAGAGGGTTTGTATATTAAAAATAATATATCATACTCATACATGA

Protein sequence

>MsaT031933.1

MKGIPKRMTGTNMLHTEEGPEPKKSNMVRPSSHGSSSTFRSWQPSWKSIDLGKQEASSSGVKASPQDKKTNNISVPKSNLGHAEKNHIIGGSASACVGVGDQATQKLDPKRLKRILANRISAQKSRLKKLDYIADLERMEKYLQDELSFLRPQVEVYKNQQWLLTMEHHQLRLEIAVREKERILQEVEIENKRAEVNRLRELQKKLVAEAQNKRVCILKIIYHTHT*