Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG031933 | XP_024634655.1 | 74.879 | 207 | 37 | 1 | 8 | 214 | 1 | 192 | 3.07e-99 | 297 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG031933 | sp|Q5JMK6|BZP06_ORYSJ | 45.263 | 95 | 52 | 0 | 108 | 202 | 137 | 231 | 1.79e-18 | 84.7 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG031933 | tr|A0A2K3MUT1|A0A2K3MUT1_TRIPR | 55.959 | 193 | 82 | 3 | 14 | 205 | 1 | 191 | 2.37e-56 | 187 |
Gene ID | Type | Classification |
---|---|---|
MsaG031933 | TF | bZIP |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000083 | MsaG031933 | 0.814986 | 2.014676e-51 | 3.893273e-49 |
MsaG000321 | MsaG031933 | 0.839912 | 2.202679e-57 | 8.575553e-55 |
MsaG001169 | MsaG031933 | 0.806460 | 1.379441e-49 | 2.164283e-47 |
MsaG003778 | MsaG031933 | 0.809197 | 3.636440e-50 | 6.090598e-48 |
MsaG005408 | MsaG031933 | 0.805462 | 2.232143e-49 | 3.420495e-47 |
MsaG006150 | MsaG031933 | 0.800912 | 1.930959e-48 | 2.665006e-46 |
MsaG006256 | MsaG031933 | 0.845975 | 5.456881e-59 | 2.582342e-56 |
MsaG006257 | MsaG031933 | 0.821310 | 7.592859e-53 | 1.728856e-50 |
MsaG006387 | MsaG031933 | 0.813056 | 5.346905e-51 | 9.843880e-49 |
MsaG006388 | MsaG031933 | 0.809761 | 2.754919e-50 | 4.677576e-48 |
MsaG006999 | MsaG031933 | 0.806764 | 1.191098e-49 | 1.882144e-47 |
MsaG007177 | MsaG031933 | 0.807770 | 7.307768e-50 | 1.182684e-47 |
MsaG007658 | MsaG031933 | 0.820128 | 1.414843e-52 | 3.122450e-50 |
MsaG009848 | MsaG031933 | 0.822302 | 4.486230e-53 | 1.048885e-50 |
MsaG010328 | MsaG031933 | 0.808555 | 4.980999e-50 | 8.214816e-48 |
MsaG010566 | MsaG031933 | 0.828900 | 1.245912e-54 | 3.494181e-52 |
MsaG011508 | MsaG031933 | 0.805262 | 2.456859e-49 | 3.747389e-47 |
MsaG011461 | MsaG031933 | 0.828386 | 1.656304e-54 | 4.578872e-52 |
MsaG011891 | MsaG031933 | 0.802281 | 1.014769e-48 | 1.444789e-46 |
MsaG012169 | MsaG031933 | 0.800408 | 2.444090e-48 | 3.335203e-46 |
MsaG012213 | MsaG031933 | 0.815551 | 1.511193e-51 | 2.962293e-49 |
MsaG012586 | MsaG031933 | 0.803171 | 6.661581e-49 | 9.680442e-47 |
MsaG041894 | MsaG031933 | 0.800758 | 2.075825e-48 | 2.855098e-46 |
MsaG042087 | MsaG031933 | 0.802057 | 1.127837e-48 | 1.597568e-46 |
MsaG042511 | MsaG031933 | 0.814995 | 2.005580e-51 | 3.876586e-49 |
MsaG042892 | MsaG031933 | 0.807430 | 8.622082e-50 | 1.384065e-47 |
MsaG043178 | MsaG031933 | 0.839372 | 3.038773e-57 | 1.163067e-54 |
MsaG043226 | MsaG031933 | 0.833420 | 9.773416e-56 | 3.123688e-53 |
MsaG043354 | MsaG031933 | 0.804776 | 3.100552e-49 | 4.676230e-47 |
MsaG045290 | MsaG031933 | 0.803947 | 4.607123e-49 | 6.816223e-47 |
MsaG046018 | MsaG031933 | 0.820429 | 1.208068e-52 | 2.687472e-50 |
MsaG046408 | MsaG031933 | 0.805779 | 1.916507e-49 | 2.958688e-47 |
MsaG046700 | MsaG031933 | 0.808789 | 4.441120e-50 | 7.365998e-48 |
MsaG046944 | MsaG031933 | 0.801417 | 1.524076e-48 | 2.127684e-46 |
MsaG006154 | MsaG031933 | 0.808080 | 6.282245e-50 | 1.024330e-47 |
MsaG009464 | MsaG031933 | 0.801286 | 1.620403e-48 | 2.255413e-46 |
MsaG008905 | MsaG031933 | 0.815199 | 1.808236e-51 | 3.513150e-49 |
MsaG011916 | MsaG031933 | 0.816157 | 1.108757e-51 | 2.207171e-49 |
MsaG018328 | MsaG031933 | 0.827008 | 3.535053e-54 | 9.401691e-52 |
MsaG018567 | MsaG031933 | 0.829161 | 1.077304e-54 | 3.043725e-52 |
MsaG020224 | MsaG031933 | 0.805690 | 2.000071e-49 | 3.081332e-47 |
MsaG027945 | MsaG031933 | 0.812276 | 7.904859e-51 | 1.427270e-48 |
MsaG028065 | MsaG031933 | 0.802523 | 9.052139e-49 | 1.296002e-46 |
MsaG032938 | MsaG031933 | 0.815269 | 1.744542e-51 | 3.395450e-49 |
MsaG031933 | MsaG034539 | 0.809191 | 3.646399e-50 | 6.106471e-48 |
MsaG031933 | MsaG031810 | 0.801083 | 1.782681e-48 | 2.469863e-46 |
MsaG031933 | MsaG033058 | 0.812661 | 6.517385e-51 | 1.188094e-48 |
MsaG031933 | MsaG037322 | 0.803810 | 4.917663e-49 | 7.252536e-47 |
MsaG014381 | MsaG031933 | 0.805403 | 2.296310e-49 | 3.514023e-47 |
MsaG014543 | MsaG031933 | 0.805266 | 2.452334e-49 | 3.740836e-47 |
MsaG017203 | MsaG031933 | 0.802919 | 7.507314e-49 | 1.084582e-46 |
MsaG017348 | MsaG031933 | 0.820961 | 9.132033e-53 | 2.060212e-50 |
MsaG017459 | MsaG031933 | 0.813043 | 5.381420e-51 | 9.904177e-49 |
MsaG018876 | MsaG031933 | 0.800133 | 2.778919e-48 | 3.768454e-46 |
MsaG019273 | MsaG031933 | 0.804284 | 3.923769e-49 | 5.850871e-47 |
MsaG019358 | MsaG031933 | 0.801683 | 1.344833e-48 | 1.888823e-46 |
MsaG020332 | MsaG031933 | 0.804337 | 3.824335e-49 | 5.709643e-47 |
MsaG020469 | MsaG031933 | 0.807859 | 6.998672e-50 | 1.135096e-47 |
MsaG020648 | MsaG031933 | 0.805290 | 2.423864e-49 | 3.699513e-47 |
MsaG020684 | MsaG031933 | 0.813348 | 4.615782e-51 | 8.559851e-49 |
MsaG021395 | MsaG031933 | 0.801008 | 1.845821e-48 | 2.553096e-46 |
MsaG023415 | MsaG031933 | 0.849558 | 5.689314e-60 | 3.037706e-57 |
MsaG023664 | MsaG031933 | 0.805268 | 2.449540e-49 | 3.736778e-47 |
MsaG023807 | MsaG031933 | 0.803838 | 4.852271e-49 | 7.160709e-47 |
MsaG023892 | MsaG031933 | 0.801156 | 1.722246e-48 | 2.390134e-46 |
MsaG026637 | MsaG031933 | 0.804749 | 3.141306e-49 | 4.734790e-47 |
MsaG027006 | MsaG031933 | 0.813641 | 3.981461e-51 | 7.438165e-49 |
MsaG027792 | MsaG031933 | 0.809735 | 2.791254e-50 | 4.736201e-48 |
MsaG027829 | MsaG031933 | 0.809415 | 3.266015e-50 | 5.499324e-48 |
MsaG027950 | MsaG031933 | 0.801510 | 1.458631e-48 | 2.040611e-46 |
MsaG028250 | MsaG031933 | 0.822213 | 4.705230e-53 | 1.097416e-50 |
MsaG028935 | MsaG031933 | 0.813798 | 3.678364e-51 | 6.899302e-49 |
MsaG028982 | MsaG031933 | 0.817139 | 6.696050e-52 | 1.367017e-49 |
MsaG030605 | MsaG031933 | 0.810202 | 2.216783e-50 | 3.804186e-48 |
MsaG030803 | MsaG031933 | 0.805649 | 2.040054e-49 | 3.139881e-47 |
MsaG031064 | MsaG031933 | 0.805755 | 1.938556e-49 | 2.991074e-47 |
MsaG031092 | MsaG031933 | 0.802721 | 8.244983e-49 | 1.185743e-46 |
MsaG035317 | MsaG031933 | 0.801939 | 1.192181e-48 | 1.684208e-46 |
MsaG035456 | MsaG031933 | 0.802104 | 1.102938e-48 | 1.563946e-46 |
MsaG035659 | MsaG031933 | 0.805317 | 2.392778e-49 | 3.654417e-47 |
MsaG036122 | MsaG031933 | 0.824473 | 1.403068e-53 | 3.478948e-51 |
MsaG036162 | MsaG031933 | 0.814851 | 2.158622e-51 | 4.157141e-49 |
MsaG037439 | MsaG031933 | 0.818464 | 3.373780e-52 | 7.126704e-50 |
MsaG040264 | MsaG031933 | 0.804459 | 3.608020e-49 | 5.401704e-47 |
MsaG040342 | MsaG031933 | 0.806052 | 1.679984e-49 | 2.610343e-47 |
MsaG040499 | MsaG031933 | 0.809829 | 2.664248e-50 | 4.531133e-48 |
MsaG040583 | MsaG031933 | 0.827270 | 3.061912e-54 | 8.203656e-52 |
MsaG040907 | MsaG031933 | 0.829286 | 1.005426e-54 | 2.850716e-52 |
MsaG041534 | MsaG031933 | 0.829209 | 1.049306e-54 | 2.968468e-52 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG031933 | MtrunA17_Chr3g0105741 | 66.667 | 75 | 21 | 3 | 22 | 95 | 9 | 80 | 2.37e-25 | 98.2 |
MsaG031933 | MtrunA17_Chr3g0105741 | 47.778 | 90 | 44 | 3 | 7 | 95 | 89 | 176 | 1.27e-15 | 72.4 |
MsaG031933 | MtrunA17_Chr2g0316551 | 41.667 | 96 | 56 | 0 | 107 | 202 | 207 | 302 | 1.37e-17 | 80.1 |
MsaG031933 | MtrunA17_Chr4g0040891 | 43.158 | 95 | 54 | 0 | 108 | 202 | 194 | 288 | 2.31e-17 | 79.3 |
MsaG031933 | MtrunA17_Chr2g0325131 | 43.158 | 95 | 54 | 0 | 108 | 202 | 191 | 285 | 3.99e-17 | 79.0 |
MsaG031933 | MtrunA17_Chr5g0432701 | 28.188 | 149 | 99 | 1 | 82 | 222 | 125 | 273 | 2.17e-11 | 62.4 |
MsaG031933 | MtrunA17_Chr3g0107491 | 38.298 | 94 | 58 | 0 | 107 | 200 | 176 | 269 | 2.99e-11 | 62.0 |
MsaG031933 | MtrunA17_Chr4g0036071 | 35.922 | 103 | 66 | 0 | 108 | 210 | 360 | 462 | 6.36e-11 | 61.2 |
MsaG031933 | MtrunA17_Chr6g0463571 | 36.792 | 106 | 67 | 0 | 107 | 212 | 150 | 255 | 9.07e-11 | 60.5 |
MsaG031933 | MtrunA17_Chr2g0329551 | 36.458 | 96 | 61 | 0 | 107 | 202 | 220 | 315 | 9.51e-11 | 60.8 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG031933 | AT2G42380.2 | 40.179 | 112 | 67 | 0 | 91 | 202 | 169 | 280 | 1.52e-18 | 82.8 |
MsaG031933 | AT3G58120.1 | 40.708 | 113 | 66 | 1 | 90 | 202 | 185 | 296 | 2.46e-18 | 82.4 |
MsaG031933 | AT2G42380.5 | 41.667 | 96 | 56 | 0 | 91 | 186 | 169 | 264 | 4.64e-16 | 75.5 |
MsaG031933 | AT3G58120.2 | 42.268 | 97 | 55 | 1 | 90 | 186 | 185 | 280 | 7.11e-16 | 75.1 |
MsaG031933 | AT2G42380.1 | 35.714 | 112 | 61 | 1 | 91 | 202 | 169 | 269 | 1.31e-13 | 68.9 |
MsaG031933 | AT5G07160.1 | 40.860 | 93 | 55 | 0 | 108 | 200 | 48 | 140 | 5.67e-12 | 62.0 |
MsaG031933 | AT1G43700.1 | 35.593 | 118 | 69 | 1 | 83 | 200 | 176 | 286 | 1.09e-11 | 63.5 |
MsaG031933 | AT1G06850.1 | 33.333 | 120 | 80 | 0 | 107 | 226 | 147 | 266 | 3.65e-11 | 62.0 |
MsaG031933 | AT2G40620.1 | 38.298 | 94 | 58 | 0 | 107 | 200 | 147 | 240 | 4.22e-11 | 62.0 |
Find 39 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
CAGCTATTTCAAGTCTTAAT+TGG | 0.226858 | 6:+40111748 | None:intergenic |
GCTCGATTATATTGCTGATT+TGG | 0.235809 | 6:-40112406 | MsaT031933.1:CDS |
GCATGACCAAGATTTGATTT+TGG | 0.261059 | 6:+40113051 | None:intergenic |
TTTGTACCAGTCATTCTCTT+TGG | 0.270280 | 6:+40113264 | None:intergenic |
GTATACCGTTTGAGCCTCTT+TGG | 0.278573 | 6:+40112952 | None:intergenic |
TTGTACCAGTCATTCTCTTT+GGG | 0.334410 | 6:+40113265 | None:intergenic |
ATAAGAGAGCTGAGGTGAAT+AGG | 0.385871 | 6:-40111594 | MsaT031933.1:CDS |
TTCGATCATGGCAACCCTCT+TGG | 0.403712 | 6:-40113161 | MsaT031933.1:CDS |
AGTGTACCAAAATCAAATCT+TGG | 0.459311 | 6:-40113057 | MsaT031933.1:CDS |
CGTCATCAACATTTCGATCA+TGG | 0.470936 | 6:-40113173 | MsaT031933.1:CDS |
GCTGAGAAGAACCACATCAT+TGG | 0.483237 | 6:-40113030 | MsaT031933.1:CDS |
TATTGCTGATTTGGAGCGGA+TGG | 0.492832 | 6:-40112397 | MsaT031933.1:CDS |
GAGCTGAGGTGAATAGGCTA+AGG | 0.500065 | 6:-40111588 | MsaT031933.1:CDS |
TGGATCCAAAGAGGCTCAAA+CGG | 0.511736 | 6:-40112957 | MsaT031933.1:intron |
AAACAAGAGGCGAGTAGCAG+TGG | 0.525757 | 6:-40113120 | MsaT031933.1:CDS |
TCTTAATTGGTGATGTTCCA+TGG | 0.529196 | 6:+40111761 | None:intergenic |
GGCATCCCAAAGAGAATGAC+TGG | 0.538746 | 6:-40113270 | MsaT031933.1:CDS |
GTGCAGAAATTCGATTTGCT+AGG | 0.542578 | 6:+40112448 | None:intergenic |
AACATGTTGCACACAGAAGA+GGG | 0.550817 | 6:-40113243 | MsaT031933.1:CDS |
AGACTTCAACTTGTGGGCGA+AGG | 0.559195 | 6:+40111808 | None:intergenic |
GATTGAGAATAAGAGAGCTG+AGG | 0.559603 | 6:-40111602 | MsaT031933.1:CDS |
GCATCTGCATGTGTTGGTGT+TGG | 0.564639 | 6:-40113000 | MsaT031933.1:CDS |
GAGAAGAACCACATCATTGG+CGG | 0.593377 | 6:-40113027 | MsaT031933.1:CDS |
ATGCACTACCGCCAATGATG+TGG | 0.601403 | 6:+40113019 | None:intergenic |
TTGCTGAAGCACAAAACAAG+AGG | 0.602002 | 6:-40111546 | MsaT031933.1:intron |
AAACATGTTGCACACAGAAG+AGG | 0.606246 | 6:-40113244 | MsaT031933.1:CDS |
GGTAGTGCATCTGCATGTGT+TGG | 0.606657 | 6:-40113006 | MsaT031933.1:CDS |
TCAGCAGTGGTTGTTGACCA+TGG | 0.606900 | 6:-40111778 | MsaT031933.1:CDS |
AGCTGAGGTGAATAGGCTAA+GGG | 0.613143 | 6:-40111587 | MsaT031933.1:CDS |
ATGGTTAGACCCTCTTCACA+TGG | 0.614802 | 6:-40113198 | MsaT031933.1:CDS |
ATTATATTGCTGATTTGGAG+CGG | 0.619255 | 6:-40112401 | MsaT031933.1:CDS |
AAAGAAAGGATACTTCAAGA+AGG | 0.624481 | 6:-40111717 | MsaT031933.1:intron |
AGGTCAATGCTCTTCCAAGA+GGG | 0.634364 | 6:+40113147 | None:intergenic |
TACTCGCCTCTTGTTTACCG+AGG | 0.637734 | 6:+40113127 | None:intergenic |
CATTGACCTCGGTAAACAAG+AGG | 0.644199 | 6:-40113133 | MsaT031933.1:CDS |
GATGACGATCCATGTGAAGA+GGG | 0.656652 | 6:+40113189 | None:intergenic |
GAGGTCAATGCTCTTCCAAG+AGG | 0.660030 | 6:+40113146 | None:intergenic |
TGATGACGATCCATGTGAAG+AGG | 0.681158 | 6:+40113188 | None:intergenic |
TCTTGGAAGAGCATTGACCT+CGG | 0.690835 | 6:-40113144 | MsaT031933.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr6 | gene | 40111413 | 40113298 | 40111413 | ID=MsaG031933 |
Chr6 | mRNA | 40111413 | 40113298 | 40111413 | ID=MsaT031933.1;Parent=MsaG031933 |
Chr6 | exon | 40111413 | 40111452 | 40111413 | ID=MsaT031933.1.exon5;Parent=MsaT031933.1 |
Chr6 | CDS | 40111413 | 40111452 | 40111413 | ID=cds.MsaT031933.1;Parent=MsaT031933.1 |
Chr6 | exon | 40111547 | 40111628 | 40111547 | ID=MsaT031933.1.exon4;Parent=MsaT031933.1 |
Chr6 | CDS | 40111547 | 40111628 | 40111547 | ID=cds.MsaT031933.1;Parent=MsaT031933.1 |
Chr6 | exon | 40111718 | 40111844 | 40111718 | ID=MsaT031933.1.exon3;Parent=MsaT031933.1 |
Chr6 | CDS | 40111718 | 40111844 | 40111718 | ID=cds.MsaT031933.1;Parent=MsaT031933.1 |
Chr6 | exon | 40112383 | 40112473 | 40112383 | ID=MsaT031933.1.exon2;Parent=MsaT031933.1 |
Chr6 | CDS | 40112383 | 40112473 | 40112383 | ID=cds.MsaT031933.1;Parent=MsaT031933.1 |
Chr6 | exon | 40112958 | 40113298 | 40112958 | ID=MsaT031933.1.exon1;Parent=MsaT031933.1 |
Chr6 | CDS | 40112958 | 40113298 | 40112958 | ID=cds.MsaT031933.1;Parent=MsaT031933.1 |
Gene Sequence |
Protein sequence |