Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG032093 | XP_039682898.1 | 62.275 | 167 | 35 | 4 | 1 | 140 | 1 | 166 | 2.75e-48 | 186 |
| MsaG032093 | XP_039682898.1 | 69.675 | 277 | 46 | 3 | 133 | 371 | 515 | 791 | 7.55e-106 | 339 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG032093 | tr|A0A2Z6NCL9|A0A2Z6NCL9_TRISU | 38.793 | 116 | 62 | 2 | 88 | 195 | 35 | 149 | 7.14e-12 | 78.6 |
| MsaG032093 | tr|A0A2Z6NCL9|A0A2Z6NCL9_TRISU | 64.413 | 281 | 52 | 5 | 133 | 369 | 397 | 673 | 1.73e-98 | 317 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG032093 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG032093 | MtrunA17_Chr3g0088371 | 67.391 | 184 | 23 | 2 | 225 | 371 | 1 | 184 | 5.19e-78 | 238 |
| MsaG032093 | MtrunA17_Chr1g0172971 | 70.085 | 117 | 23 | 3 | 1 | 105 | 1 | 117 | 1.55e-40 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 54 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTCGCTTCTTTGACCTAAT+TGG | 0.186128 | 6:+44844924 | None:intergenic |
| TTCTTTGACCTAATTGGATT+TGG | 0.299426 | 6:+44844930 | None:intergenic |
| TGTTCTTGAGGTAGGGAAAA+AGG | 0.342722 | 6:-44845078 | MsaT032093.1:CDS |
| TTTGCTTCTTCCGAGCTATA+AGG | 0.345757 | 6:+44844862 | None:intergenic |
| GGTTTCGAATGTTGATGTTT+TGG | 0.354913 | 6:-44845495 | MsaT032093.1:CDS |
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | 6:+44847324 | None:intergenic |
| AAGAAAGACCAAATCCAATT+AGG | 0.397771 | 6:-44844938 | MsaT032093.1:CDS |
| TTTGGTCTTTCTTTGAACTT+TGG | 0.398856 | 6:+44844948 | None:intergenic |
| GAGGAGTTTCTTATTTCTAA+AGG | 0.403538 | 6:-44845053 | MsaT032093.1:CDS |
| CACGTCGTATGTTTGAGAAC+CGG | 0.404458 | 6:-44845370 | MsaT032093.1:CDS |
| CCATGATTCTTTATCACTAA+AGG | 0.406174 | 6:+44847561 | None:intergenic |
| GATTATGATCTTCAAACCTT+GGG | 0.413114 | 6:+44845447 | None:intergenic |
| TTCCTTCAAATACTATTATG+AGG | 0.414157 | 6:-44845618 | MsaT032093.1:CDS |
| ATAACACTGGATTCTCTGTT+AGG | 0.417808 | 6:-44847480 | MsaT032093.1:CDS |
| GGGTTGTCACAGTCGACATT+AGG | 0.419786 | 6:-44845412 | MsaT032093.1:CDS |
| CGATTATGATCTTCAAACCT+TGG | 0.425681 | 6:+44845446 | None:intergenic |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 6:-44847282 | MsaT032093.1:CDS |
| CGCTTTAAAACATATTGTGC+AGG | 0.447633 | 6:+44845269 | None:intergenic |
| ATCTGAGAGTGGACAATTGA+CGG | 0.452529 | 6:-44847626 | MsaT032093.1:CDS |
| TGAATGTCTATAAGATATAT+AGG | 0.472794 | 6:-44847233 | MsaT032093.1:intron |
| ACGTCGTATGTTTGAGAACC+GGG | 0.475165 | 6:-44845369 | MsaT032093.1:CDS |
| CTCCTCATAATAGTATTTGA+AGG | 0.485089 | 6:+44845616 | None:intergenic |
| TTTAATTATGCATATAACAC+TGG | 0.485542 | 6:-44847493 | MsaT032093.1:CDS |
| TATGAGGAGGACACAACAAA+AGG | 0.490908 | 6:-44845602 | MsaT032093.1:CDS |
| TAAAGCTGGAATATATTTGA+AGG | 0.493683 | 6:-44847268 | MsaT032093.1:CDS |
| CTCAACGGTGTTCTTGAGGT+AGG | 0.495390 | 6:-44845086 | MsaT032093.1:CDS |
| ATCATAATCGACTTGATGAA+AGG | 0.507955 | 6:-44845433 | MsaT032093.1:CDS |
| TAAAGAATCATGGAAGTCAT+TGG | 0.509974 | 6:-44847551 | MsaT032093.1:CDS |
| GATCTTCAAACCTTGGGGAA+AGG | 0.519004 | 6:+44845453 | None:intergenic |
| TCAACGGTGTTCTTGAGGTA+GGG | 0.519325 | 6:-44845085 | MsaT032093.1:CDS |
| TCATAATCGACTTGATGAAA+GGG | 0.526860 | 6:-44845432 | MsaT032093.1:CDS |
| ACAAAATAAGAAATCAGATA+AGG | 0.530342 | 6:-44847431 | MsaT032093.1:intron |
| GACAGAAATTCCTTATAGCT+CGG | 0.533906 | 6:-44844872 | MsaT032093.1:CDS |
| ATGAGGAGGACACAACAAAA+GGG | 0.542065 | 6:-44845601 | MsaT032093.1:CDS |
| CCTTTAGTGATAAAGAATCA+TGG | 0.543522 | 6:-44847561 | MsaT032093.1:CDS |
| TGAAAATTCAGTTATTGATG+CGG | 0.565546 | 6:-44847602 | MsaT032093.1:CDS |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.567394 | 6:-44847386 | MsaT032093.1:intron |
| TACACGTGTTTGCGCAGATG+CGG | 0.571672 | 6:-44845126 | MsaT032093.1:CDS |
| ATTTCTCAACGGTGTTCTTG+AGG | 0.577665 | 6:-44845090 | MsaT032093.1:CDS |
| AGCTGGAATATATTTGAAGG+TGG | 0.586074 | 6:-44847265 | MsaT032093.1:CDS |
| GCTGTCTGTGAACCATCTGT+TGG | 0.593238 | 6:-44844978 | MsaT032093.1:CDS |
| TGAAGACTTAGAAGGCGTAA+CGG | 0.598405 | 6:+44845010 | None:intergenic |
| TTGAAGGAAACCTTTCCCCA+AGG | 0.600566 | 6:-44845463 | MsaT032093.1:CDS |
| ACGTGTTTGCGCAGATGCGG+AGG | 0.605934 | 6:-44845123 | MsaT032093.1:CDS |
| CTTCAAATACTATTATGAGG+AGG | 0.607664 | 6:-44845615 | MsaT032093.1:CDS |
| ATGTTGATAATGATAATGAG+AGG | 0.609132 | 6:-44845517 | MsaT032093.1:CDS |
| ATATCAACTTCATCTGAGAG+TGG | 0.614980 | 6:-44847637 | MsaT032093.1:CDS |
| TGTTGATAATGATAATGAGA+GGG | 0.618346 | 6:-44845516 | MsaT032093.1:CDS |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | 6:+44847339 | None:intergenic |
| ATTATGATCTTCAAACCTTG+GGG | 0.630358 | 6:+44845448 | None:intergenic |
| TTTGGTGCAGTACCAACAGA+TGG | 0.635771 | 6:+44844966 | None:intergenic |
| GCATCTGAAATATTTCTCAA+CGG | 0.661104 | 6:-44845101 | MsaT032093.1:CDS |
| CATGTCGACATAAGAAACCC+CGG | 0.663279 | 6:+44845351 | None:intergenic |
| CGTCGTATGTTTGAGAACCG+GGG | 0.667385 | 6:-44845368 | MsaT032093.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 44844833 | 44847668 | 44844833 | ID=MsaG032093 |
| Chr6 | mRNA | 44844833 | 44847668 | 44844833 | ID=MsaT032093.1;Parent=MsaG032093 |
| Chr6 | exon | 44844833 | 44845153 | 44844833 | ID=MsaT032093.1.exon4;Parent=MsaT032093.1 |
| Chr6 | CDS | 44844833 | 44845153 | 44844833 | ID=cds.MsaT032093.1;Parent=MsaT032093.1 |
| Chr6 | exon | 44845256 | 44845652 | 44845256 | ID=MsaT032093.1.exon3;Parent=MsaT032093.1 |
| Chr6 | CDS | 44845256 | 44845652 | 44845256 | ID=cds.MsaT032093.1;Parent=MsaT032093.1 |
| Chr6 | exon | 44847234 | 44847400 | 44847234 | ID=MsaT032093.1.exon2;Parent=MsaT032093.1 |
| Chr6 | CDS | 44847234 | 44847400 | 44847234 | ID=cds.MsaT032093.1;Parent=MsaT032093.1 |
| Chr6 | exon | 44847432 | 44847668 | 44847432 | ID=MsaT032093.1.exon1;Parent=MsaT032093.1 |
| Chr6 | CDS | 44847432 | 44847668 | 44847432 | ID=cds.MsaT032093.1;Parent=MsaT032093.1 |
| Gene Sequence |
| Protein sequence |
