AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG032329

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032329 MtrunA17_Chr2g0281511 72.388 134 24 1 1 134 1 121 1.32e-62 187
MsaG032329 MtrunA17_Chr2g0309121 69.403 134 27 2 1 134 1 120 4.73e-58 176
MsaG032329 MtrunA17_Chr3g0081891 67.742 93 17 1 1 93 1 80 4.55e-35 116
MsaG032329 MtrunA17_Chr7g0276661 36.029 136 76 5 7 135 38 169 1.77e-20 82.0
MsaG032329 MtrunA17_Chr5g0430431 41.667 60 35 0 69 128 194 253 1.09e-12 63.2
MsaG032329 MtrunA17_Chr8g0356961 36.275 102 50 3 24 123 77 165 1.26e-11 58.9
MsaG032329 MtrunA17_Chr8g0372431 24.545 110 70 1 24 133 6 102 8.51e-11 55.1
MsaG032329 MtrunA17_Chr7g0228081 30.263 76 53 0 58 133 17 92 9.04e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATGTTACTGCTTCTATTT+TGG 0.123735 6:+49482550 MsaT032329.1:CDS
GAAGTCAATGATCCTGAATT+AGG 0.214972 6:+49482491 MsaT032329.1:CDS
AAAATGTCGATGTTCATTAT+TGG 0.262235 6:+49482522 MsaT032329.1:CDS
TACCAGTTTCTTCGTCAAAA+AGG 0.263872 6:-49482739 None:intergenic
TGTATGCTACCGTCGTTAAC+TGG 0.282411 6:+49482906 MsaT032329.1:CDS
GTATGACTTTGTTCTTGAAA+AGG 0.406559 6:+49482829 MsaT032329.1:CDS
ACGGTAGCATACATCATATT+AGG 0.417507 6:-49482896 None:intergenic
AAGTCAATGATCCTGAATTA+GGG 0.438648 6:+49482492 MsaT032329.1:CDS
TATGACTTTGTTCTTGAAAA+GGG 0.441122 6:+49482830 MsaT032329.1:CDS
GTATGCTACCGTCGTTAACT+GGG 0.475193 6:+49482907 MsaT032329.1:CDS
CTTGAAAAGGGCGTAAAAGT+TGG 0.486889 6:+49482842 MsaT032329.1:CDS
GCATGCATAAGAAGTACATA+GGG 0.550408 6:+49482798 MsaT032329.1:CDS
AGCATGCATAAGAAGTACAT+AGG 0.566294 6:+49482797 MsaT032329.1:CDS
AGAAGTACATAGGGAGAGGA+TGG 0.574105 6:+49482807 MsaT032329.1:CDS
CATAAGAAGTACATAGGGAG+AGG 0.578101 6:+49482803 MsaT032329.1:CDS
GTAGCATACATCATATTAGG+AGG 0.617573 6:-49482893 None:intergenic
TATGCTACCGTCGTTAACTG+GGG 0.696887 6:+49482908 MsaT032329.1:CDS
AAATTATCCCCAGTTAACGA+CGG 0.712817 6:-49482915 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 49482473 49482934 49482473 ID=MsaG032329
Chr6 mRNA 49482473 49482934 49482473 ID=MsaT032329.1;Parent=MsaG032329
Chr6 exon 49482473 49482640 49482473 ID=MsaT032329.1.exon1;Parent=MsaT032329.1
Chr6 CDS 49482473 49482640 49482473 ID=cds.MsaT032329.1;Parent=MsaT032329.1
Chr6 exon 49482695 49482934 49482695 ID=MsaT032329.1.exon2;Parent=MsaT032329.1
Chr6 CDS 49482695 49482934 49482695 ID=cds.MsaT032329.1;Parent=MsaT032329.1
Gene Sequence

>MsaT032329.1

ATGAATGAGACTGGAGTTGAAGTCAATGATCCTGAATTAGGGAATGTTCAAAATGTCGATGTTCATTATTGGAAGTATAATGTTACTGCTTCTATTTTGGATGGAAACAATGTTTTAGTTAGTGCCATTTACGAAGCATTTTTGTTGTTTTATGTTGTTAAGTTGTTGGGTGTTGCTGAGAATGTTTTGTATATCAATCAATCTAAAATTCTCCTTTTTGACGAAGAAACTGGTAATTCTTATGACTGTGATGTTCACATTGCGCCAAAAAGCATGCATAAGAAGTACATAGGGAGAGGATGGTATGACTTTGTTCTTGAAAAGGGCGTAAAAGTTGGTGACATTATTTTGTTCACAGTCAATAATCCTCCTAATATGATGTATGCTACCGTCGTTAACTGGGGATAA
Protein sequence

>MsaT032329.1

MNETGVEVNDPELGNVQNVDVHYWKYNVTASILDGNNVLVSAIYEAFLLFYVVKLLGVAENVLYINQSKILLFDEETGNSYDCDVHIAPKSMHKKYIGRGWYDFVLEKGVKVGDIILFTVNNPPNMMYATVVNWG*