Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG032412 | AFK38694.1 | 90.323 | 31 | 3 | 0 | 24 | 54 | 82 | 112 | 1.01e-12 | 70.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG032412 | sp|Q2V9B0|MY1R1_SOLTU | 83.871 | 31 | 5 | 0 | 24 | 54 | 93 | 123 | 1.10e-14 | 68.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG032412 | tr|I3SEK2|I3SEK2_MEDTR | 90.323 | 31 | 3 | 0 | 24 | 54 | 82 | 112 | 4.81e-13 | 70.5 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG032412 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG032412 | MtrunA17_Chr4g0059701 | 90.323 | 31 | 3 | 0 | 24 | 54 | 82 | 112 | 1.53e-16 | 70.5 |
| MsaG032412 | MtrunA17_Chr8g0389241 | 80.645 | 31 | 6 | 0 | 24 | 54 | 103 | 133 | 2.40e-15 | 67.0 |
| MsaG032412 | MtrunA17_Chr2g0331031 | 80.645 | 31 | 6 | 0 | 24 | 54 | 107 | 137 | 2.82e-15 | 67.4 |
| MsaG032412 | MtrunA17_Chr5g0415191 | 80.645 | 31 | 6 | 0 | 24 | 54 | 100 | 130 | 2.82e-15 | 67.0 |
| MsaG032412 | MtrunA17_Chr6g0488471 | 80.645 | 31 | 6 | 0 | 24 | 54 | 95 | 125 | 3.34e-14 | 63.9 |
| MsaG032412 | MtrunA17_Chr4g0055931 | 77.419 | 31 | 7 | 0 | 24 | 54 | 93 | 123 | 4.44e-14 | 63.9 |
| MsaG032412 | MtrunA17_Chr3g0104071 | 77.419 | 31 | 7 | 0 | 24 | 54 | 145 | 175 | 7.89e-14 | 63.2 |
| MsaG032412 | MtrunA17_Chr5g0429521 | 77.419 | 31 | 7 | 0 | 24 | 54 | 130 | 160 | 1.05e-13 | 62.8 |
| MsaG032412 | MtrunA17_Chr4g0062741 | 77.419 | 31 | 7 | 0 | 24 | 54 | 136 | 166 | 1.48e-13 | 62.4 |
| MsaG032412 | MtrunA17_Chr6g0452791 | 86.207 | 29 | 4 | 0 | 26 | 54 | 80 | 108 | 5.11e-13 | 59.3 |
| MsaG032412 | MtrunA17_Chr7g0241761 | 74.194 | 31 | 8 | 0 | 24 | 54 | 138 | 168 | 7.26e-13 | 60.5 |
| MsaG032412 | MtrunA17_Chr1g0210471 | 71.875 | 32 | 9 | 0 | 24 | 55 | 122 | 153 | 8.63e-13 | 59.7 |
| MsaG032412 | MtrunA17_Chr3g0092621 | 86.207 | 29 | 4 | 0 | 26 | 54 | 84 | 112 | 1.24e-12 | 58.5 |
| MsaG032412 | MtrunA17_Chr1g0162081 | 74.194 | 31 | 8 | 0 | 24 | 54 | 120 | 150 | 2.85e-12 | 58.9 |
| MsaG032412 | MtrunA17_Chr3g0135671 | 70.968 | 31 | 9 | 0 | 24 | 54 | 89 | 119 | 3.00e-12 | 58.2 |
| MsaG032412 | MtrunA17_Chr5g0436501 | 74.194 | 31 | 8 | 0 | 24 | 54 | 123 | 153 | 3.26e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG032412 | AT3G16350.1 | 80.645 | 31 | 6 | 0 | 24 | 54 | 135 | 165 | 3.65e-15 | 67.4 |
| MsaG032412 | AT5G47390.1 | 83.871 | 31 | 5 | 0 | 24 | 54 | 94 | 124 | 4.14e-15 | 67.4 |
| MsaG032412 | AT1G74840.2 | 77.419 | 31 | 7 | 0 | 24 | 54 | 94 | 124 | 5.15e-15 | 66.2 |
| MsaG032412 | AT1G74840.1 | 77.419 | 31 | 7 | 0 | 24 | 54 | 94 | 124 | 6.71e-15 | 66.2 |
| MsaG032412 | AT5G56840.1 | 77.419 | 31 | 7 | 0 | 24 | 54 | 89 | 119 | 1.38e-14 | 64.7 |
| MsaG032412 | AT1G19000.2 | 77.419 | 31 | 7 | 0 | 24 | 54 | 100 | 130 | 3.78e-14 | 64.3 |
| MsaG032412 | AT1G19000.1 | 77.419 | 31 | 7 | 0 | 24 | 54 | 100 | 130 | 3.78e-14 | 64.3 |
| MsaG032412 | AT1G70000.1 | 77.419 | 31 | 7 | 0 | 24 | 54 | 96 | 126 | 6.42e-14 | 63.5 |
| MsaG032412 | AT1G70000.2 | 77.419 | 31 | 7 | 0 | 24 | 54 | 96 | 126 | 6.42e-14 | 63.5 |
| MsaG032412 | AT5G58900.1 | 77.419 | 31 | 7 | 0 | 24 | 54 | 139 | 169 | 1.63e-13 | 62.4 |
| MsaG032412 | AT1G49010.1 | 77.419 | 31 | 7 | 0 | 24 | 54 | 132 | 162 | 2.13e-13 | 62.4 |
| MsaG032412 | AT2G38090.1 | 70.968 | 31 | 9 | 0 | 24 | 54 | 139 | 169 | 2.12e-12 | 59.3 |
| MsaG032412 | AT5G05790.2 | 74.194 | 31 | 8 | 0 | 24 | 54 | 129 | 159 | 3.90e-12 | 58.5 |
| MsaG032412 | AT5G05790.1 | 74.194 | 31 | 8 | 0 | 24 | 54 | 129 | 159 | 3.90e-12 | 58.5 |
| MsaG032412 | AT3G11280.2 | 74.194 | 31 | 8 | 0 | 24 | 54 | 125 | 155 | 4.70e-12 | 58.2 |
| MsaG032412 | AT3G11280.1 | 74.194 | 31 | 8 | 0 | 24 | 54 | 125 | 155 | 4.70e-12 | 58.2 |
| MsaG032412 | AT5G08520.1 | 70.968 | 31 | 9 | 0 | 24 | 54 | 116 | 146 | 1.28e-11 | 57.4 |
| MsaG032412 | AT5G04760.1 | 65.625 | 32 | 11 | 0 | 24 | 55 | 97 | 128 | 1.35e-11 | 56.6 |
| MsaG032412 | AT5G61620.1 | 67.742 | 31 | 10 | 0 | 24 | 54 | 106 | 136 | 1.63e-11 | 57.0 |
Find 11 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGTTGGATTGCAGAAAGT+TGG | 0.394483 | 6:+51867506 | MsaT032412.1:CDS |
| AAGGATCTAGATGTCAAGTT+TGG | 0.401683 | 6:+51865957 | None:intergenic |
| AGAAAGTTGGAAAAGGTGAT+TGG | 0.477943 | 6:+51867519 | MsaT032412.1:CDS |
| TTGTGCTCTTCCCGCGTCCA+CGG | 0.487114 | 6:-51867476 | None:intergenic |
| GATCAGGGATTCCGTGGACG+CGG | 0.527878 | 6:+51867465 | MsaT032412.1:intron |
| GGATTGCAGAAAGTTGGAAA+AGG | 0.553347 | 6:+51867512 | MsaT032412.1:CDS |
| GACGCGCTAATCTGCTTGAA+AGG | 0.576197 | 6:+51866018 | MsaT032412.1:CDS |
| ATCAGGGATTCCGTGGACGC+GGG | 0.598661 | 6:+51867466 | MsaT032412.1:intron |
| GTGATTGGAGAGGAATCTCA+AGG | 0.619264 | 6:+51867534 | MsaT032412.1:CDS |
| TGAATGGATCAGGGATTCCG+TGG | 0.629323 | 6:+51867459 | MsaT032412.1:intron |
| GTTGGAAAAGGTGATTGGAG+AGG | 0.636411 | 6:+51867524 | MsaT032412.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 51865967 | 51867565 | 51865967 | ID=MsaG032412 |
| Chr6 | mRNA | 51865967 | 51867565 | 51865967 | ID=MsaT032412.1;Parent=MsaG032412 |
| Chr6 | exon | 51865967 | 51866039 | 51865967 | ID=MsaT032412.1.exon1;Parent=MsaT032412.1 |
| Chr6 | CDS | 51865967 | 51866039 | 51865967 | ID=cds.MsaT032412.1;Parent=MsaT032412.1 |
| Chr6 | exon | 51867471 | 51867565 | 51867471 | ID=MsaT032412.1.exon2;Parent=MsaT032412.1 |
| Chr6 | CDS | 51867471 | 51867565 | 51867471 | ID=cds.MsaT032412.1;Parent=MsaT032412.1 |
| Gene Sequence |
| Protein sequence |
