AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG032451


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032451 MtrunA17_Chr6g0467041 69.452 347 56 4 1 317 1 327 4.86e-166 464
MsaG032451 MtrunA17_Chr8g0378061 75.347 288 38 5 23 277 23 310 2.33e-145 418
MsaG032451 MtrunA17_Chr8g0378061 74.603 63 16 0 257 319 395 457 4.13e-22 96.3
MsaG032451 MtrunA17_Chr2g0330821 56.522 276 102 5 1 261 60 332 2.33e-95 286
MsaG032451 MtrunA17_Chr2g0330841 54.545 231 71 5 82 278 41 271 2.29e-69 221
MsaG032451 MtrunA17_Chr5g0410881 49.763 211 92 4 22 218 32 242 9.74e-63 207
MsaG032451 MtrunA17_Chr8g0379701 37.892 351 168 7 15 315 5 355 1.84e-62 203
MsaG032451 MtrunA17_Chr4g0039891 44.498 209 104 3 20 216 10 218 9.04e-54 183
MsaG032451 MtrunA17_Chr3g0135831 35.294 306 184 6 19 317 43 341 1.30e-51 174
MsaG032451 MtrunA17_Chr1g0204741 42.793 222 110 6 7 214 20 238 3.17e-50 169
MsaG032451 MtrunA17_Chr7g0260331 45.146 206 103 4 19 214 46 251 5.06e-50 170
MsaG032451 MtrunA17_Chr1g0185131 44.000 175 90 2 22 195 32 199 4.14e-46 159
MsaG032451 MtrunA17_Chr4g0071051 44.624 186 99 2 19 203 98 280 5.48e-46 160
MsaG032451 MtrunA17_Chr8g0392001 48.205 195 88 6 20 201 20 214 2.49e-45 158
MsaG032451 MtrunA17_Chr3g0134031 65.686 102 35 0 12 113 14 115 3.62e-44 155
MsaG032451 MtrunA17_Chr5g0402291 60.377 106 42 0 17 122 4 109 6.22e-42 147
MsaG032451 MtrunA17_Chr6g0485571 39.252 214 103 2 20 219 9 209 2.87e-41 146
MsaG032451 MtrunA17_Chr5g0441531 67.742 93 30 0 21 113 24 116 3.24e-41 144
MsaG032451 MtrunA17_Chr5g0397881 58.333 108 45 0 6 113 8 115 7.07e-41 145
MsaG032451 MtrunA17_Chr2g0284481 57.407 108 46 0 12 119 5 112 5.84e-40 144
MsaG032451 MtrunA17_Chr6g0472421 61.290 93 36 0 21 113 32 124 1.74e-37 136
MsaG032451 MtrunA17_Chr7g0257331 59.140 93 38 0 21 113 21 113 1.95e-37 133
MsaG032451 MtrunA17_Chr1g0203601 58.947 95 39 0 21 115 17 111 8.06e-37 134
MsaG032451 MtrunA17_Chr4g0047421 57.732 97 37 1 21 113 32 128 6.42e-34 123
MsaG032451 MtrunA17_Chr8g0375041 54.839 93 42 0 21 113 8 100 2.56e-32 120
MsaG032451 MtrunA17_Chr1g0179321 64.516 62 22 0 20 81 47 108 5.49e-25 97.1
MsaG032451 MtrunA17_Chr4g0023771 59.420 69 27 1 12 79 4 72 4.71e-20 84.0
MsaG032451 MtrunA17_Chr1g0179311 30.147 136 92 2 82 214 14 149 1.23e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032451 AT4G13980.1 53.419 234 107 2 1 232 1 234 6.71e-81 253
MsaG032451 AT1G32330.1 49.296 213 93 4 1 198 16 228 1.53e-58 196
MsaG032451 AT1G32330.2 49.296 213 93 4 1 198 16 228 1.73e-58 195
MsaG032451 AT4G17750.1 47.222 216 93 4 22 216 52 267 1.66e-57 193
MsaG032451 AT3G02990.1 42.918 233 120 4 8 228 9 240 7.05e-57 191
MsaG032451 AT5G16820.1 47.867 211 95 5 20 216 25 234 1.12e-56 191
MsaG032451 AT5G16820.2 47.867 211 95 5 20 216 25 234 1.12e-56 191
MsaG032451 AT5G45710.3 35.647 317 175 7 14 315 5 307 9.11e-53 177
MsaG032451 AT5G45710.1 35.647 317 175 7 14 315 5 307 9.11e-53 177
MsaG032451 AT4G18880.1 47.393 211 101 3 16 220 9 215 8.55e-51 173
MsaG032451 AT2G26150.1 42.342 222 120 4 19 232 41 262 5.79e-50 169
MsaG032451 AT2G26150.4 42.342 222 120 4 19 232 41 262 5.79e-50 169
MsaG032451 AT3G51910.1 42.654 211 104 5 22 226 29 228 1.93e-49 166
MsaG032451 AT3G22830.1 41.429 210 109 4 19 214 58 267 8.59e-47 163
MsaG032451 AT5G03720.2 42.079 202 109 3 20 213 53 254 3.98e-46 161
MsaG032451 AT5G03720.1 42.079 202 109 3 20 213 53 254 3.98e-46 161
MsaG032451 AT3G24520.1 45.652 184 77 4 20 202 15 176 1.59e-45 157
MsaG032451 AT1G67970.1 44.615 195 95 5 20 204 17 208 2.20e-44 155
MsaG032451 AT1G46264.1 61.607 112 33 1 21 122 33 144 1.47e-43 153
MsaG032451 AT5G43840.1 42.246 187 96 3 21 205 18 194 1.58e-42 148
MsaG032451 AT5G54070.1 42.162 185 101 2 20 203 69 248 2.10e-40 144
MsaG032451 AT3G63350.1 35.238 210 135 1 19 227 25 234 1.02e-39 141
MsaG032451 AT4G11660.1 60.000 100 40 0 14 113 51 150 5.30e-38 139
MsaG032451 AT4G36990.1 56.481 108 45 1 8 115 2 107 1.03e-37 136
MsaG032451 AT5G62020.1 49.580 119 59 1 1 118 1 119 3.74e-37 135
MsaG032451 AT2G41690.1 50.435 115 57 0 22 136 40 154 4.39e-36 130
MsaG032451 AT4G18870.1 46.591 88 47 0 22 109 146 233 2.53e-22 95.1
MsaG032451 AT4G18870.1 44.792 96 52 1 14 109 5 99 8.04e-22 93.6
MsaG032451 AT1G77570.1 43.478 92 48 3 19 106 22 113 5.42e-17 77.0
MsaG032451 AT2G26150.3 32.704 159 99 4 82 232 49 207 1.43e-15 76.3
MsaG032451 AT2G26150.2 32.704 159 99 4 82 232 49 207 1.43e-15 76.3
MsaG032451 AT4G19630.1 34.021 97 61 1 19 112 8 104 3.43e-13 66.2
MsaG032451 AT5G45710.4 25.333 225 139 7 106 315 1 211 2.52e-11 63.2
MsaG032451 AT5G45710.2 25.333 225 139 7 106 315 1 211 2.52e-11 63.2

Find 74 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCCAATGCTGCCCTTTC+TGG -0.010683 6:+52916980 None:intergenic
CTTCTTTGTGAAGGCTCTTC+AGG 0.202681 6:-52916813 MsaT032451.1:CDS
TGCAGGCTTTCCTCATCTTC+AGG 0.204361 6:+52916629 None:intergenic
ATAATTATAGCTTAAGAGTT+TGG 0.284728 6:+52916916 None:intergenic
GAAATTGCTGTGTTTGAAAT+AGG 0.299469 6:+52918186 None:intergenic
CCACAGATGAGATCGTTTCT+TGG 0.319260 6:-52918273 MsaT032451.1:CDS
CAAAATCAAATCCATGGATT+TGG 0.330894 6:-52916762 MsaT032451.1:CDS
CTTTCTGAAGGAGAACTTAC+TGG 0.346936 6:-52916599 MsaT032451.1:CDS
ACGGAGTGAATGATGTGTTT+TGG 0.356521 6:-52916484 MsaT032451.1:CDS
ATAACAAAAGCTTCATCGTT+TGG 0.362747 6:-52918243 MsaT032451.1:CDS
GCAGGCTTTCCTCATCTTCA+GGG 0.373078 6:+52916630 None:intergenic
AGAATAACGCGAGAGAATTC+AGG 0.380452 6:+52918214 None:intergenic
ACGTTACCTGAAGCCTCCTT+TGG 0.398346 6:+52916530 None:intergenic
ACCAAGCTACCTTCGGCAAC+AGG 0.417304 6:+52916692 None:intergenic
AGCTTAAGAGTTTGGATTGA+AGG 0.419735 6:+52916924 None:intergenic
TAAGAAGAGGGGATTGTCTC+AGG 0.434641 6:-52916729 MsaT032451.1:CDS
TTCCCGCAAAATCAAATCCA+TGG 0.455530 6:-52916768 MsaT032451.1:CDS
GAGTTTATTTGAGATATCTT+CGG 0.461136 6:+52916652 None:intergenic
TGGAAACTTATAATAAGAAG+AGG 0.463694 6:-52916742 MsaT032451.1:CDS
TCTGCTTCCGTCGGCAGCAG+TGG 0.466428 6:+52918348 None:intergenic
GCTGATCCAAAGGAGGCTTC+AGG 0.466668 6:-52916536 MsaT032451.1:CDS
CCACAGTCATAGTCATCCTC+CGG 0.469987 6:-52917017 MsaT032451.1:CDS
GCCGAGCAGCTGCTTCGACT+TGG 0.471786 6:+52916507 None:intergenic
GGAAACTTATAATAAGAAGA+GGG 0.473221 6:-52916741 MsaT032451.1:CDS
AAAATCAGATCCTGATCGTT+GGG 0.473476 6:-52917110 MsaT032451.1:CDS
TTGGCAAACTCCCAACGATC+AGG 0.478885 6:+52917100 None:intergenic
TTGCTGTGTTTGAAATAGGC+TGG 0.480359 6:+52918190 None:intergenic
GCTTAAGAGTTTGGATTGAA+GGG 0.480417 6:+52916925 None:intergenic
ATTATAAGTTTCCAAATCCA+TGG 0.481800 6:+52916751 None:intergenic
AGATCCAGAAAGGGCAGCAT+TGG 0.494908 6:-52916984 MsaT032451.1:CDS
CGACGGAAGCAGAGAAGGTA+TGG 0.499479 6:-52918338 MsaT032451.1:CDS
TGAGACAGAAAAGCTTCAGC+TGG 0.500611 6:-52916891 MsaT032451.1:CDS
ATTGAGCTGACGGATGAAGC+TGG 0.506271 6:+52918162 None:intergenic
GCCTGTTGCCGAAGGTAGCT+TGG 0.510527 6:-52916693 MsaT032451.1:CDS
ATATCTTCGGCAACATTCCA+TGG 0.514486 6:+52916665 None:intergenic
TTTGCAGAACTTCTTTGTGA+AGG 0.519196 6:-52916822 MsaT032451.1:CDS
TTTCTGAAGGAGAACTTACT+GGG 0.521375 6:-52916598 MsaT032451.1:CDS
TGGATCAACCAAGCTACCTT+CGG 0.537647 6:+52916685 None:intergenic
AACAGCATGGACTTTCAGTA+AGG 0.541453 6:+52916453 None:intergenic
GAAAATCAGATCCTGATCGT+TGG 0.543561 6:-52917111 MsaT032451.1:CDS
TCATACGTCTTCTGAAGAAA+CGG 0.543831 6:+52918313 None:intergenic
GAAACTTATAATAAGAAGAG+GGG 0.544462 6:-52916740 MsaT032451.1:CDS
GTCGAAGCAGCTGCTCGGCA+CGG 0.548207 6:-52916503 MsaT032451.1:CDS
TCAGCGGCGATTGGAAGGCA+TGG 0.549084 6:-52916861 MsaT032451.1:CDS
TCCAAGTCGAAGCAGCTGCT+CGG 0.549998 6:-52916508 MsaT032451.1:CDS
CCGGAGGATGACTATGACTG+TGG 0.551542 6:+52917017 None:intergenic
CACAGTCATAGTCATCCTCC+GGG 0.559092 6:-52917016 MsaT032451.1:CDS
TTCTGCTGTAGATCCAGAAA+GGG 0.565003 6:-52916993 MsaT032451.1:CDS
GTTCTGCTGTAGATCCAGAA+AGG 0.569845 6:-52916994 MsaT032451.1:CDS
AAGGTAGCTTGGTTGATCCA+TGG 0.570487 6:-52916682 MsaT032451.1:CDS
GACTGTGGATAGGTTTCTTG+CGG 0.574041 6:+52917032 None:intergenic
TGCTGTGTTTGAAATAGGCT+GGG 0.577118 6:+52918191 None:intergenic
AAATAAACTCCCTGAAGATG+AGG 0.580535 6:-52916639 MsaT032451.1:CDS
GCTGCCGACGGAAGCAGAGA+AGG 0.581055 6:-52918343 MsaT032451.1:CDS
AGAAACGATCTCATCTGTGG+TGG 0.582151 6:+52918276 None:intergenic
GATTGGAAGGCATGGAGAAG+AGG 0.593047 6:-52916853 MsaT032451.1:CDS
CACATGCAGCCTGTTGCCGA+AGG 0.596050 6:-52916701 MsaT032451.1:CDS
TCTTCATTGTCTGAACAGCA+TGG 0.597736 6:+52916440 None:intergenic
TTACATAGGTATTGAGCTGA+CGG 0.598207 6:+52918152 None:intergenic
TCATCAGCCACTGCTGCCGA+CGG 0.606067 6:-52918355 MsaT032451.1:CDS
TCTGGATCTACAGCAGAACC+CGG 0.614712 6:+52916998 None:intergenic
GGATGACTATGACTGTGGAT+AGG 0.617132 6:+52917022 None:intergenic
CATACCTTCTCTGCTTCCGT+CGG 0.623107 6:+52918339 None:intergenic
GAAACGATCTCATCTGTGGT+GGG 0.626600 6:+52918277 None:intergenic
CCAAGAAACGATCTCATCTG+TGG 0.633047 6:+52918273 None:intergenic
ATGCTGTTCAGACAATGAAG+AGG 0.637659 6:-52916438 MsaT032451.1:CDS
TATTAAGCTAGCTGATCCAA+AGG 0.648739 6:-52916546 MsaT032451.1:CDS
ATAACGCGAGAGAATTCAGG+AGG 0.662865 6:+52918217 None:intergenic
TCTTCAGAAGACGTATGACA+TGG 0.665030 6:-52918308 MsaT032451.1:CDS
TAAGCTAGCTGATCCAAAGG+AGG 0.675297 6:-52916543 MsaT032451.1:CDS
TTCTGAAGGAGAACTTACTG+GGG 0.695684 6:-52916597 MsaT032451.1:CDS
GCAGTGGCTGATGATGATTG+TGG 0.706595 6:+52918364 None:intergenic
GGATCTACAGCAGAACCCGG+AGG 0.744225 6:+52917001 None:intergenic
TAACGCGAGAGAATTCAGGA+GGG 0.754081 6:+52918218 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 52916424 52918398 52916424 ID=MsaG032451
Chr6 mRNA 52916424 52918398 52916424 ID=MsaT032451.1;Parent=MsaG032451
Chr6 exon 52916424 52917140 52916424 ID=MsaT032451.1.exon2;Parent=MsaT032451.1
Chr6 CDS 52916424 52917140 52916424 ID=cds.MsaT032451.1;Parent=MsaT032451.1
Chr6 exon 52918156 52918398 52918156 ID=MsaT032451.1.exon1;Parent=MsaT032451.1
Chr6 CDS 52918156 52918398 52918156 ID=cds.MsaT032451.1;Parent=MsaT032451.1
Gene Sequence

>MsaT032451.1

ATGAATTCTGCACCACAATCATCATCAGCCACTGCTGCCGACGGAAGCAGAGAAGGTATGGCACCGTTTCTTCAGAAGACGTATGACATGGTAGATGATCCCACCACAGATGAGATCGTTTCTTGGAGCTCTGATAACAAAAGCTTCATCGTTTGGAACCCTCCTGAATTCTCTCGCGTTATTCTCCCAGCCTATTTCAAACACAGCAATTTCTCCAGCTTCATCCGTCAGCTCAATACCTATGGATTTCGAAAATCAGATCCTGATCGTTGGGAGTTTGCCAATGAAAAATTTATAAAAGATCAAAAACATCTTCTGACGGATATACACCGCAAGAAACCTATCCACAGTCATAGTCATCCTCCGGGTTCTGCTGTAGATCCAGAAAGGGCAGCATTGGAGAAAGAAATAGAGAAGCTGTGTCGCAAGAAAAATGCCCTTCAATCCAAACTCTTAAGCTATAATTATCTTGAGACAGAAAAGCTTCAGCTGGAAGATTTTCAGCGGCGATTGGAAGGCATGGAGAAGAGGCAGACAAATTTGCAGAACTTCTTTGTGAAGGCTCTTCAGGATTCTTTCATTGTTGAACTTCTTTCCCGCAAAATCAAATCCATGGATTTGGAAACTTATAATAAGAAGAGGGGATTGTCTCAGGTTGATCACATGCAGCCTGTTGCCGAAGGTAGCTTGGTTGATCCATGGAATGTTGCCGAAGATATCTCAAATAAACTCCCTGAAGATGAGGAAAGCCTGCAGAAAAATCTTTCTGAAGGAGAACTTACTGGGGCATCTTTTACTAATGTGGCTAATGAGGCTATTAAGCTAGCTGATCCAAAGGAGGCTTCAGGTAACGTCCAAGTCGAAGCAGCTGCTCGGCACGGAGTGAATGATGTGTTTTGGGAACGATTCCTTACTGAAAGTCCATGCTGTTCAGACAATGAAGAGGAAATGTTCAACTAA

Protein sequence

>MsaT032451.1

MNSAPQSSSATAADGSREGMAPFLQKTYDMVDDPTTDEIVSWSSDNKSFIVWNPPEFSRVILPAYFKHSNFSSFIRQLNTYGFRKSDPDRWEFANEKFIKDQKHLLTDIHRKKPIHSHSHPPGSAVDPERAALEKEIEKLCRKKNALQSKLLSYNYLETEKLQLEDFQRRLEGMEKRQTNLQNFFVKALQDSFIVELLSRKIKSMDLETYNKKRGLSQVDHMQPVAEGSLVDPWNVAEDISNKLPEDEESLQKNLSEGELTGASFTNVANEAIKLADPKEASGNVQVEAAARHGVNDVFWERFLTESPCCSDNEEEMFN*