AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG032454


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032454 MtrunA17_Chr6g0467041 72.256 328 62 3 1 299 1 328 2.38e-168 469
MsaG032454 MtrunA17_Chr8g0378061 76.619 278 22 2 23 257 23 300 1.52e-146 420
MsaG032454 MtrunA17_Chr8g0378061 86.364 44 6 0 257 300 414 457 4.12e-18 84.3
MsaG032454 MtrunA17_Chr2g0330821 53.746 307 117 8 1 298 60 350 1.68e-95 286
MsaG032454 MtrunA17_Chr2g0330841 56.164 219 52 3 83 257 42 260 5.57e-70 222
MsaG032454 MtrunA17_Chr5g0410881 50.237 211 91 4 22 218 32 242 3.34e-63 207
MsaG032454 MtrunA17_Chr8g0379701 47.826 207 98 3 15 216 5 206 9.50e-58 190
MsaG032454 MtrunA17_Chr4g0039891 44.498 209 104 3 20 216 10 218 5.32e-54 183
MsaG032454 MtrunA17_Chr1g0204741 43.049 223 110 6 7 215 20 239 3.40e-50 169
MsaG032454 MtrunA17_Chr7g0260331 45.673 208 101 5 19 215 46 252 1.60e-49 168
MsaG032454 MtrunA17_Chr3g0135831 43.137 204 110 3 19 217 43 245 2.36e-49 168
MsaG032454 MtrunA17_Chr1g0185131 40.271 221 112 4 22 233 32 241 1.75e-45 157
MsaG032454 MtrunA17_Chr4g0071051 43.548 186 101 2 19 203 98 280 1.75e-44 156
MsaG032454 MtrunA17_Chr3g0134031 65.686 102 35 0 12 113 14 115 7.85e-44 153
MsaG032454 MtrunA17_Chr8g0392001 46.667 195 91 6 20 201 20 214 1.62e-43 152
MsaG032454 MtrunA17_Chr5g0402291 60.377 106 42 0 17 122 4 109 2.38e-41 144
MsaG032454 MtrunA17_Chr6g0485571 40.196 204 115 2 20 219 9 209 2.85e-41 146
MsaG032454 MtrunA17_Chr5g0441531 67.742 93 30 0 21 113 24 116 9.35e-41 142
MsaG032454 MtrunA17_Chr5g0397881 58.333 108 45 0 6 113 8 115 2.72e-40 144
MsaG032454 MtrunA17_Chr2g0284481 57.407 108 46 0 12 119 5 112 2.96e-39 141
MsaG032454 MtrunA17_Chr6g0472421 61.290 93 36 0 21 113 32 124 3.75e-37 135
MsaG032454 MtrunA17_Chr7g0257331 58.065 93 39 0 21 113 21 113 6.67e-37 131
MsaG032454 MtrunA17_Chr1g0203601 58.947 95 39 0 21 115 17 111 2.52e-36 132
MsaG032454 MtrunA17_Chr4g0047421 56.701 97 38 1 21 113 32 128 3.40e-33 121
MsaG032454 MtrunA17_Chr8g0375041 54.839 93 42 0 21 113 8 100 1.86e-31 117
MsaG032454 MtrunA17_Chr1g0179321 62.903 62 23 0 20 81 47 108 9.91e-25 95.9
MsaG032454 MtrunA17_Chr4g0023771 61.644 73 26 2 12 82 4 76 3.84e-21 86.7
MsaG032454 MtrunA17_Chr1g0179311 28.655 171 107 4 83 243 15 180 3.20e-13 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032454 AT4G13980.1 53.755 253 107 3 1 243 1 253 8.20e-86 265
MsaG032454 AT4G17750.1 47.685 216 92 4 22 216 52 267 4.85e-58 194
MsaG032454 AT1G32330.1 45.957 235 108 5 1 216 16 250 1.69e-57 192
MsaG032454 AT1G32330.2 45.957 235 108 5 1 216 16 250 2.00e-57 192
MsaG032454 AT5G16820.1 48.341 211 94 5 20 216 25 234 2.11e-57 192
MsaG032454 AT5G16820.2 48.341 211 94 5 20 216 25 234 2.11e-57 192
MsaG032454 AT3G02990.1 42.918 233 120 4 8 228 9 240 3.29e-57 191
MsaG032454 AT5G45710.3 38.730 315 155 9 14 299 5 310 3.12e-54 180
MsaG032454 AT5G45710.1 38.730 315 155 9 14 299 5 310 3.12e-54 180
MsaG032454 AT4G18880.1 48.598 214 94 4 16 220 9 215 2.21e-51 174
MsaG032454 AT2G26150.1 42.342 222 120 4 19 232 41 262 9.50e-50 168
MsaG032454 AT2G26150.4 42.342 222 120 4 19 232 41 262 9.50e-50 168
MsaG032454 AT3G51910.1 41.706 211 106 5 22 226 29 228 1.64e-46 158
MsaG032454 AT3G22830.1 39.815 216 117 3 19 221 58 273 2.24e-46 161
MsaG032454 AT5G03720.2 41.584 202 110 3 20 213 53 254 3.74e-45 158
MsaG032454 AT5G03720.1 41.584 202 110 3 20 213 53 254 3.74e-45 158
MsaG032454 AT3G24520.1 45.087 173 75 3 20 191 15 168 1.99e-43 151
MsaG032454 AT1G67970.1 44.792 192 93 5 20 201 17 205 4.09e-43 152
MsaG032454 AT1G46264.1 61.607 112 33 1 21 122 33 144 4.65e-43 151
MsaG032454 AT5G43840.1 41.176 187 98 3 21 205 18 194 8.55e-41 143
MsaG032454 AT5G54070.1 42.623 183 99 2 20 201 69 246 5.95e-40 142
MsaG032454 AT3G63350.1 31.802 283 159 8 19 297 25 277 3.81e-39 139
MsaG032454 AT4G11660.1 60.000 100 40 0 14 113 51 150 1.88e-37 137
MsaG032454 AT4G36990.1 45.455 176 61 4 8 151 2 174 5.47e-37 133
MsaG032454 AT5G62020.1 49.580 119 59 1 1 118 1 119 1.13e-36 133
MsaG032454 AT2G41690.1 51.304 115 56 0 22 136 40 154 3.21e-36 130
MsaG032454 AT4G18870.1 45.455 88 48 0 22 109 146 233 1.71e-21 92.4
MsaG032454 AT4G18870.1 44.792 96 52 1 14 109 5 99 3.10e-21 91.7
MsaG032454 AT1G77570.1 41.304 92 50 3 19 106 22 113 1.78e-15 72.8
MsaG032454 AT2G26150.3 32.278 158 99 4 83 232 50 207 2.13e-15 75.5
MsaG032454 AT2G26150.2 32.278 158 99 4 83 232 50 207 2.13e-15 75.5
MsaG032454 AT5G45710.4 30.493 223 117 10 106 299 1 214 2.06e-13 68.9
MsaG032454 AT5G45710.2 30.493 223 117 10 106 299 1 214 2.06e-13 68.9
MsaG032454 AT4G19630.1 32.990 97 62 1 19 112 8 104 5.43e-12 62.8

Find 71 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCCAATGCTGCCCTTTC+TGG -0.010683 6:+52981014 None:intergenic
CTTCTTTGAGAAGGCTCTTC+AGG 0.202772 6:-52980847 MsaT032454.1:CDS
ATAATTATAGCTTAGGAGTT+TGG 0.257010 6:+52980950 None:intergenic
GAAATTGCTGTGTTTGAAAT+AGG 0.280393 6:+52982559 None:intergenic
CCACAGATGAGATCGTTTCT+TGG 0.319260 6:-52982646 MsaT032454.1:CDS
CAAAATCGAATCCATGGATT+TGG 0.330894 6:-52980796 MsaT032454.1:CDS
ACGGAGTGAATGATGTGTTT+TGG 0.356521 6:-52980575 MsaT032454.1:CDS
ATAACAAAAGCTTCATCGTT+TGG 0.362747 6:-52982616 MsaT032454.1:CDS
AGAAGAACGCGAGAGAATTC+AGG 0.373908 6:+52982587 None:intergenic
ACGTTACCTGAAGCCTCCTT+TGG 0.398346 6:+52980621 None:intergenic
TAAGAAAAGGCGATTGTCTC+AGG 0.414889 6:-52980763 MsaT032454.1:CDS
ACCAAGCTACCTTCGGCAAC+AGG 0.417714 6:+52980726 None:intergenic
AGCTTAGGAGTTTGGATTGA+AGG 0.419735 6:+52980958 None:intergenic
TGCTGTGTTTGAAATAGGTT+GGG 0.448167 6:+52982564 None:intergenic
TATCAAGATAATTATAGCTT+AGG 0.451761 6:+52980943 None:intergenic
TTGCTGTGTTTGAAATAGGT+TGG 0.457272 6:+52982563 None:intergenic
TGGAAACTTATAATAAGAAA+AGG 0.461564 6:-52980776 MsaT032454.1:CDS
TCTGCTTCCGTCGGCAGCAG+TGG 0.466428 6:+52982721 None:intergenic
CCACAGTCATAGTCATCCTC+CGG 0.469987 6:-52981051 MsaT032454.1:CDS
GCCGAGCAGCTGCTTCGACT+TGG 0.471786 6:+52980598 None:intergenic
GCCTGTTGCCGAAGGTAGCT+TGG 0.473073 6:-52980727 MsaT032454.1:CDS
GAAAGCAGATCCTGATCGTT+GGG 0.475772 6:-52981144 MsaT032454.1:CDS
TTGGCAAACTCCCAACGATC+AGG 0.478885 6:+52981134 None:intergenic
GCTTAGGAGTTTGGATTGAA+GGG 0.480417 6:+52980959 None:intergenic
ATTATAAGTTTCCAAATCCA+TGG 0.481800 6:+52980785 None:intergenic
GCTGATCCAAAGGAGGCTTC+AGG 0.482903 6:-52980627 MsaT032454.1:CDS
GAAGCCTCCTTTGGATCAGC+GGG 0.484852 6:+52980630 None:intergenic
TTTGCAAAACTTCTTTGAGA+AGG 0.487416 6:-52980856 MsaT032454.1:CDS
AGATCCAGAAAGGGCAGCAT+TGG 0.494908 6:-52981018 MsaT032454.1:CDS
TGATACAGAAAAGCTTCAGC+TGG 0.497298 6:-52980925 MsaT032454.1:CDS
CGACGGAAGCAGAGAAGGTA+TGG 0.499479 6:-52982711 MsaT032454.1:CDS
ATTGAGCTGACGGATGAAGC+TGG 0.506271 6:+52982535 None:intergenic
TTCTCTCAGAATCTTTCTGA+AGG 0.510971 6:-52980684 MsaT032454.1:CDS
TTCCCGCAAAATCGAATCCA+TGG 0.512391 6:-52980802 MsaT032454.1:CDS
AACTGACCCCGCTGATCCAA+AGG 0.526560 6:-52980637 MsaT032454.1:CDS
CTTTCTGAAGGAGAACTGAC+AGG 0.534056 6:-52980672 MsaT032454.1:CDS
GAGAGAAAACATTGCTAGGA+AGG 0.540423 6:+52980700 None:intergenic
AACAGCATGGACTTTCAGTA+AGG 0.541453 6:+52980544 None:intergenic
TCATACGTCTTCTGAAGAAA+CGG 0.543831 6:+52982686 None:intergenic
GGAAAGCAGATCCTGATCGT+TGG 0.545857 6:-52981145 MsaT032454.1:CDS
GTCGAAGCAGCTGCTCGGCA+CGG 0.548207 6:-52980594 MsaT032454.1:CDS
TCAGCGGCGATTGGATGGCA+TGG 0.549084 6:-52980895 MsaT032454.1:CDS
TCCAAGTCGAAGCAGCTGCT+CGG 0.549998 6:-52980599 MsaT032454.1:CDS
CCGGAGGATGACTATGACTG+TGG 0.551542 6:+52981051 None:intergenic
AAGGTCGACCAAGCTACCTT+CGG 0.554405 6:+52980719 None:intergenic
CACAGTCATAGTCATCCTCC+GGG 0.559092 6:-52981050 MsaT032454.1:CDS
TTCTGCTGTAGATCCAGAAA+GGG 0.565003 6:-52981027 MsaT032454.1:CDS
GTTCTGCTGTAGATCCAGAA+AGG 0.569845 6:-52981028 MsaT032454.1:CDS
TTCTGAGAGAAAACATTGCT+AGG 0.570976 6:+52980696 None:intergenic
GACTGTGGATAGGTTTCTTG+CGG 0.574041 6:+52981066 None:intergenic
GCTGCCGACGGAAGCAGAGA+AGG 0.581055 6:-52982716 MsaT032454.1:CDS
AGAAACGATCTCATCTGTGG+TGG 0.582151 6:+52982649 None:intergenic
GATTGGATGGCATGGAGAAG+AGG 0.590608 6:-52980887 MsaT032454.1:CDS
TGAAGCCTCCTTTGGATCAG+CGG 0.591974 6:+52980629 None:intergenic
CACATGCAGCCTGTTGCCGA+AGG 0.596050 6:-52980735 MsaT032454.1:CDS
TTACATAGGTATTGAGCTGA+CGG 0.598207 6:+52982525 None:intergenic
TCATCAGCCACTGCTGCCGA+CGG 0.606067 6:-52982728 MsaT032454.1:CDS
TCTGGATCTACAGCAGAACC+CGG 0.614712 6:+52981032 None:intergenic
GGATGACTATGACTGTGGAT+AGG 0.617132 6:+52981056 None:intergenic
TCTTCATTGTCTGAACAGCA+TGG 0.622573 6:+52980531 None:intergenic
CATACCTTCTCTGCTTCCGT+CGG 0.623107 6:+52982712 None:intergenic
GAAACGATCTCATCTGTGGT+GGG 0.626600 6:+52982650 None:intergenic
AGAACGCGAGAGAATTCAGG+AGG 0.628084 6:+52982590 None:intergenic
CCAAGAAACGATCTCATCTG+TGG 0.633047 6:+52982646 None:intergenic
ATGCTGTTCAGACAATGAAG+AGG 0.637659 6:-52980529 MsaT032454.1:CDS
TGACCCCGCTGATCCAAAGG+AGG 0.662370 6:-52980634 MsaT032454.1:CDS
GAACGCGAGAGAATTCAGGA+GGG 0.662504 6:+52982591 None:intergenic
TCTTCAGAAGACGTATGACA+TGG 0.665030 6:-52982681 MsaT032454.1:CDS
GCAGTGGCTGATGATGATTG+TGG 0.706595 6:+52982737 None:intergenic
AAGCCTCCTTTGGATCAGCG+GGG 0.731334 6:+52980631 None:intergenic
GGATCTACAGCAGAACCCGG+AGG 0.744225 6:+52981035 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 52980515 52982771 52980515 ID=MsaG032454
Chr6 mRNA 52980515 52982771 52980515 ID=MsaT032454.1;Parent=MsaG032454
Chr6 exon 52980515 52981174 52980515 ID=MsaT032454.1.exon2;Parent=MsaT032454.1
Chr6 CDS 52980515 52981174 52980515 ID=cds.MsaT032454.1;Parent=MsaT032454.1
Chr6 exon 52982529 52982771 52982529 ID=MsaT032454.1.exon1;Parent=MsaT032454.1
Chr6 CDS 52982529 52982771 52982529 ID=cds.MsaT032454.1;Parent=MsaT032454.1
Gene Sequence

>MsaT032454.1

ATGAATTCTGCACCACAATCATCATCAGCCACTGCTGCCGACGGAAGCAGAGAAGGTATGGCACCGTTTCTTCAGAAGACGTATGACATGGTAGATGATCCCACCACAGATGAGATCGTTTCTTGGAGCTCTGATAACAAAAGCTTCATCGTTTGGAACCCTCCTGAATTCTCTCGCGTTCTTCTCCCAACCTATTTCAAACACAGCAATTTCTCCAGCTTCATCCGTCAGCTCAATACCTATGTATTTCGGAAAGCAGATCCTGATCGTTGGGAGTTTGCCAATGAAAAATTTATAAAAGATCAAAAACATCTTCTGACGGATATACACCGCAAGAAACCTATCCACAGTCATAGTCATCCTCCGGGTTCTGCTGTAGATCCAGAAAGGGCAGCATTGGAGAAAGAAATAGAGAAGCTGTTGCGCGAGAAAAATGCCCTTCAATCCAAACTCCTAAGCTATAATTATCTTGATACAGAAAAGCTTCAGCTGGAAGATTTTCAGCGGCGATTGGATGGCATGGAGAAGAGGCAGACAAATTTGCAAAACTTCTTTGAGAAGGCTCTTCAGGATTCTTTTATTGTTGAACTACTTTCCCGCAAAATCGAATCCATGGATTTGGAAACTTATAATAAGAAAAGGCGATTGTCTCAGGTTGATCACATGCAGCCTGTTGCCGAAGGTAGCTTGGTCGACCTTCCTAGCAATGTTTTCTCTCAGAATCTTTCTGAAGGAGAACTGACAGGAATACAGACAAGAACTGACCCCGCTGATCCAAAGGAGGCTTCAGGTAACGTCCAAGTCGAAGCAGCTGCTCGGCACGGAGTGAATGATGTGTTTTGGGAACAATTCCTTACTGAAAGTCCATGCTGTTCAGACAATGAAGAGGCATTGTCCAACTAA

Protein sequence

>MsaT032454.1

MNSAPQSSSATAADGSREGMAPFLQKTYDMVDDPTTDEIVSWSSDNKSFIVWNPPEFSRVLLPTYFKHSNFSSFIRQLNTYVFRKADPDRWEFANEKFIKDQKHLLTDIHRKKPIHSHSHPPGSAVDPERAALEKEIEKLLREKNALQSKLLSYNYLDTEKLQLEDFQRRLDGMEKRQTNLQNFFEKALQDSFIVELLSRKIESMDLETYNKKRRLSQVDHMQPVAEGSLVDLPSNVFSQNLSEGELTGIQTRTDPADPKEASGNVQVEAAARHGVNDVFWEQFLTESPCCSDNEEALSN*