AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG032456


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032456 MtrunA17_Chr8g0343441 67.797 59 19 0 16 74 198 256 1.67e-24 91.7
MsaG032456 MtrunA17_Chr2g0329821 63.793 58 21 0 16 73 193 250 4.17e-22 85.5
MsaG032456 MtrunA17_Chr1g0150821 65.455 55 18 1 20 74 210 263 7.56e-21 82.4
MsaG032456 MtrunA17_Chr2g0300241 60.000 60 21 2 17 74 195 253 4.52e-19 77.4
MsaG032456 MtrunA17_Chr4g0006381 61.818 55 20 1 20 74 189 242 5.39e-19 77.0
MsaG032456 MtrunA17_Chr4g0076771 59.615 52 21 0 19 70 201 252 2.05e-18 75.9
MsaG032456 MtrunA17_Chr7g0242511 58.182 55 22 1 20 74 189 242 2.33e-18 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032456 AT5G20510.2 61.017 59 22 1 16 74 162 219 2.75e-21 83.2
MsaG032456 AT5G20510.1 61.017 59 22 1 16 74 203 260 6.14e-21 82.8
MsaG032456 AT5G26210.1 64.286 56 19 1 18 73 200 254 5.66e-20 80.5
MsaG032456 AT3G42790.1 61.017 59 22 1 16 74 193 250 7.68e-20 79.7
MsaG032456 AT2G02470.1 61.818 55 20 1 20 74 203 256 2.16e-19 79.0
MsaG032456 AT2G02470.2 61.818 55 20 1 20 74 194 247 2.44e-19 78.6
MsaG032456 AT1G14510.1 61.538 52 20 0 20 71 198 249 1.87e-18 76.3
MsaG032456 AT5G05610.2 58.824 51 21 0 16 66 184 234 2.34e-17 73.2
MsaG032456 AT5G05610.1 58.824 51 21 0 16 66 184 234 2.34e-17 73.2
MsaG032456 AT3G11200.1 54.902 51 23 0 16 66 189 239 3.60e-16 70.1
MsaG032456 AT3G11200.2 54.902 51 23 0 16 66 176 226 5.59e-16 69.3

Find 20 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGAAAATCCAGAATTTATC+AGG 0.249988 6:+53061530 None:intergenic
ACACACGCCCATGAGAGATA+TGG 0.451540 6:-53061624 None:intergenic
AATATGACCCTGATAAATTC+TGG 0.489252 6:-53061538 MsaT032456.1:CDS
AAGTTCTTCCATATCTCTCA+TGG 0.494470 6:+53061616 None:intergenic
TCGATATGCTTAACCCTACC+AGG 0.508444 6:+53061461 None:intergenic
GAGGAAGATGAAGATGAAGA+CGG 0.529823 6:-53061590 MsaT032456.1:CDS
TCGGTGACATTTGTGAGAGG+TGG 0.533723 6:-53061511 MsaT032456.1:CDS
TTTGTGAGAGGTGGTTCCAT+GGG 0.542875 6:-53061502 MsaT032456.1:CDS
CTCTTGTAGTCATTGCATGA+TGG 0.544814 6:+53061425 None:intergenic
GTGTGAATATCACCCCTGGT+AGG 0.570139 6:-53061475 MsaT032456.1:CDS
TGTGAATATCACCCCTGGTA+GGG 0.574077 6:-53061474 MsaT032456.1:CDS
TGATATTCACACATTTCCCA+TGG 0.578749 6:+53061486 None:intergenic
ATTTGTGAGAGGTGGTTCCA+TGG 0.579292 6:-53061503 MsaT032456.1:CDS
AGTTCTTCCATATCTCTCAT+GGG 0.595111 6:+53061617 None:intergenic
GAGAACTCATGCGGTGCATG+CGG 0.601432 6:-53061566 MsaT032456.1:CDS
CGAAAATCCAGAATTTATCA+GGG 0.613625 6:+53061531 None:intergenic
CGATATGCTTAACCCTACCA+GGG 0.644955 6:+53061462 None:intergenic
AAATGTGTGAATATCACCCC+TGG 0.663451 6:-53061479 MsaT032456.1:CDS
GAAGACGGAGAGAACTCATG+CGG 0.708786 6:-53061575 MsaT032456.1:CDS
GATATGCTTAACCCTACCAG+GGG 0.729993 6:+53061463 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 53061412 53061636 53061412 ID=MsaG032456
Chr6 mRNA 53061412 53061636 53061412 ID=MsaT032456.1;Parent=MsaG032456
Chr6 exon 53061412 53061636 53061412 ID=MsaT032456.1.exon1;Parent=MsaT032456.1
Chr6 CDS 53061412 53061636 53061412 ID=cds.MsaT032456.1;Parent=MsaT032456.1
Gene Sequence

>MsaT032456.1

ATGAGAGATATGGAAGAACTTTTTGAGGAAGATGAAGATGAAGACGGAGAGAACTCATGCGGTGCATGCGGTGAGCAATATGACCCTGATAAATTCTGGATTTTCGGTGACATTTGTGAGAGGTGGTTCCATGGGAAATGTGTGAATATCACCCCTGGTAGGGTTAAGCATATCGATATATACAAGTGTCCATCATGCAATGACTACAAGAGAGATCGCCCTTGA

Protein sequence

>MsaT032456.1

MRDMEELFEEDEDEDGENSCGACGEQYDPDKFWIFGDICERWFHGKCVNITPGRVKHIDIYKCPSCNDYKRDRP*