AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG032458


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032458 MtrunA17_Chr8g0343441 72.340 47 13 0 13 59 198 244 1.03e-19 78.6
MsaG032458 MtrunA17_Chr2g0329821 68.085 47 15 0 13 59 193 239 1.32e-18 75.5
MsaG032458 MtrunA17_Chr1g0150821 72.093 43 12 0 17 59 210 252 6.02e-18 73.9
MsaG032458 MtrunA17_Chr4g0076771 65.909 44 15 0 16 59 201 244 1.55e-17 72.8
MsaG032458 MtrunA17_Chr7g0242511 65.116 43 15 0 17 59 189 231 3.37e-17 71.6
MsaG032458 MtrunA17_Chr4g0006381 65.116 43 15 0 17 59 189 231 4.02e-17 71.6
MsaG032458 MtrunA17_Chr2g0300241 62.500 48 16 1 14 59 195 242 1.18e-15 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032458 AT5G20510.2 65.957 47 16 0 13 59 162 208 3.08e-18 74.3
MsaG032458 AT5G20510.1 65.957 47 16 0 13 59 203 249 4.80e-18 74.7
MsaG032458 AT3G42790.1 65.957 47 16 0 13 59 193 239 1.11e-17 73.6
MsaG032458 AT5G26210.1 68.889 45 14 0 15 59 200 244 1.31e-17 73.6
MsaG032458 AT5G05610.2 59.574 47 19 0 13 59 184 230 5.90e-17 71.2
MsaG032458 AT5G05610.1 59.574 47 19 0 13 59 184 230 5.90e-17 71.2
MsaG032458 AT2G02470.1 67.442 43 14 0 17 59 203 245 1.27e-16 70.9
MsaG032458 AT2G02470.2 67.442 43 14 0 17 59 194 236 1.52e-16 70.5
MsaG032458 AT1G14510.1 67.442 43 14 0 17 59 198 240 2.74e-16 69.7
MsaG032458 AT3G11200.1 57.447 47 20 0 13 59 189 235 2.05e-15 67.4
MsaG032458 AT3G11200.2 57.447 47 20 0 13 59 176 222 2.87e-15 67.0

Find 15 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAAATCCAGAATTTATC+AGG 0.245351 6:+53084000 None:intergenic
TCGATATGCTTAACCCTAGC+AGG 0.459889 6:+53083931 None:intergenic
GAGGAAGATGAAGATGAAGA+AGG 0.473888 6:-53084060 MsaT032458.1:CDS
AATATGACCCTGATAAATTC+TGG 0.489252 6:-53084008 MsaT032458.1:CDS
GTGTGAATATCACCCCTGCT+AGG 0.514666 6:-53083945 MsaT032458.1:CDS
TCTGTGACATTTGTGAGAGA+TGG 0.533709 6:-53083981 MsaT032458.1:CDS
TGTGAATATCACCCCTGCTA+GGG 0.571594 6:-53083944 MsaT032458.1:CDS
TGATATTCACACATTTCCCA+TGG 0.578749 6:+53083956 None:intergenic
CGATATGCTTAACCCTAGCA+GGG 0.587671 6:+53083932 None:intergenic
TTTGTGAGAGATGGTTCCAT+GGG 0.600358 6:-53083972 MsaT032458.1:CDS
ATTTGTGAGAGATGGTTCCA+TGG 0.610050 6:-53083973 MsaT032458.1:CDS
AGAAAATCCAGAATTTATCA+GGG 0.630317 6:+53084001 None:intergenic
GAAGAAGGAGAGAACTCATG+TGG 0.669374 6:-53084045 MsaT032458.1:CDS
GAGAACTCATGTGGTGCATG+CGG 0.697071 6:-53084036 MsaT032458.1:CDS
GATATGCTTAACCCTAGCAG+GGG 0.713226 6:+53083933 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 53083918 53084097 53083918 ID=MsaG032458
Chr6 mRNA 53083918 53084097 53083918 ID=MsaT032458.1;Parent=MsaG032458
Chr6 exon 53083918 53084097 53083918 ID=MsaT032458.1.exon1;Parent=MsaT032458.1
Chr6 CDS 53083918 53084097 53083918 ID=cds.MsaT032458.1;Parent=MsaT032458.1
Gene Sequence

>MsaT032458.1

ATGGAAGAACTTTTTGAGGAAGATGAAGATGAAGAAGGAGAGAACTCATGTGGTGCATGCGGTGAGCAATATGACCCTGATAAATTCTGGATTTTCTGTGACATTTGTGAGAGATGGTTCCATGGGAAATGTGTGAATATCACCCCTGCTAGGGTTAAGCATATCGATATATACAAGTGA

Protein sequence

>MsaT032458.1

MEELFEEDEDEEGENSCGACGEQYDPDKFWIFCDICERWFHGKCVNITPARVKHIDIYK*