Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| MsaG032465 | TF | TCP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG032465 | MtrunA17_Chr1g0204001 | 59.184 | 49 | 19 | 1 | 2 | 49 | 43 | 91 | 7.30e-12 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG032465 | AT5G51910.1 | 58.333 | 48 | 19 | 1 | 3 | 49 | 37 | 84 | 5.69e-11 | 55.8 |
| MsaG032465 | AT5G51910.2 | 58.333 | 48 | 19 | 1 | 3 | 49 | 37 | 84 | 5.69e-11 | 55.8 |
Find 15 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCAGCTTGACGAGATCT+TGG | 0.365424 | 6:-53136463 | MsaT032465.1:CDS |
| AAGTCTGATGGAGAAACAAT+AGG | 0.400346 | 6:-53136436 | None:intergenic |
| TTGCTGACAGTGTGACTCTT+CGG | 0.478708 | 6:+53136502 | None:intergenic |
| TTCGTGTGACGGTCTTCTGA+TGG | 0.489473 | 6:+53136540 | None:intergenic |
| TGACCAAGATCTCGTCAAGC+TGG | 0.502061 | 6:+53136460 | None:intergenic |
| TGTCAGCAACATGTGCATCT+AGG | 0.504146 | 6:-53136488 | MsaT032465.1:CDS |
| AGTCTGATGGAGAAACAATA+GGG | 0.517180 | 6:-53136435 | None:intergenic |
| AAAACAGACGTTGAACCCAC+TGG | 0.536961 | 6:-53136595 | MsaT032465.1:CDS |
| GTCCCTCCACCTTCGTGTGA+CGG | 0.543321 | 6:+53136529 | None:intergenic |
| AGACCGTCACACGAAGGTGG+AGG | 0.561466 | 6:-53136532 | MsaT032465.1:CDS |
| GATCTTGGTCACAAGTCTGA+TGG | 0.572653 | 6:-53136448 | MsaT032465.1:CDS |
| GACCGTCACACGAAGGTGGA+GGG | 0.603506 | 6:-53136531 | MsaT032465.1:CDS |
| AGAAGACCGTCACACGAAGG+TGG | 0.622175 | 6:-53136535 | MsaT032465.1:CDS |
| GGAACCACAAAAGACAGTGT+CGG | 0.676346 | 6:-53136574 | MsaT032465.1:CDS |
| ATCAGAAGACCGTCACACGA+AGG | 0.685962 | 6:-53136538 | MsaT032465.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 53136437 | 53136622 | 53136437 | ID=MsaG032465 |
| Chr6 | mRNA | 53136437 | 53136622 | 53136437 | ID=MsaT032465.1;Parent=MsaG032465 |
| Chr6 | exon | 53136437 | 53136622 | 53136437 | ID=MsaT032465.1.exon1;Parent=MsaT032465.1 |
| Chr6 | CDS | 53136437 | 53136622 | 53136437 | ID=cds.MsaT032465.1;Parent=MsaT032465.1 |
| Gene Sequence |
| Protein sequence |
