AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG032466


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032466 MtrunA17_Chr8g0343441 73.029 241 54 2 5 245 15 244 1.91e-100 293
MsaG032466 MtrunA17_Chr2g0300241 60.082 243 77 3 5 245 18 242 2.34e-84 252
MsaG032466 MtrunA17_Chr2g0329821 61.345 238 80 2 8 245 14 239 6.25e-84 251
MsaG032466 MtrunA17_Chr1g0150821 61.826 241 88 2 5 245 16 252 4.32e-81 244
MsaG032466 MtrunA17_Chr4g0076771 55.187 241 96 2 5 245 16 244 3.76e-78 236
MsaG032466 MtrunA17_Chr4g0006381 51.440 243 92 8 5 245 13 231 4.18e-64 200
MsaG032466 MtrunA17_Chr7g0242511 47.303 241 105 6 5 245 13 231 2.35e-61 193
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032466 AT5G20510.1 58.091 241 94 2 5 245 16 249 1.04e-83 251
MsaG032466 AT3G42790.1 58.091 241 84 4 5 245 16 239 5.99e-81 243
MsaG032466 AT1G14510.1 57.261 241 88 2 5 245 15 240 1.01e-76 233
MsaG032466 AT2G02470.1 57.261 241 93 3 5 245 15 245 7.71e-74 225
MsaG032466 AT2G02470.2 57.261 241 84 4 5 245 15 236 3.63e-72 221
MsaG032466 AT5G05610.2 51.867 241 92 5 5 245 14 230 3.68e-70 216
MsaG032466 AT5G05610.1 51.867 241 92 5 5 245 14 230 3.68e-70 216
MsaG032466 AT5G26210.1 61.570 242 82 2 5 246 15 245 2.95e-69 214
MsaG032466 AT5G20510.2 55.660 212 87 2 34 245 4 208 1.57e-66 206
MsaG032466 AT3G11200.1 51.867 241 95 5 5 245 16 235 9.54e-63 197
MsaG032466 AT5G20510.3 64.901 151 53 0 5 155 16 166 7.45e-62 192
MsaG032466 AT3G11200.2 52.830 212 79 6 34 245 32 222 9.88e-55 176
MsaG032466 AT2G02470.3 65.753 146 47 1 5 150 15 157 9.04e-53 172

Find 72 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTGGATTTGCTGCCTTTA+TGG 0.143405 6:+53165042 None:intergenic
GGAAGTCAATTTACCTTCTA+TGG 0.184392 6:-53165327 MsaT032466.1:CDS
CCGAAAATCCAGAATTTATC+AGG 0.216776 6:+53164498 None:intergenic
TTGACTTCCCATTGCTCATT+AGG 0.233040 6:+53165341 None:intergenic
TTGCCATGGCACACTACTTT+GGG 0.283032 6:-53165193 MsaT032466.1:CDS
TGACTTCCCATTGCTCATTA+GGG 0.286910 6:+53165342 None:intergenic
AGCATAGGCTCGGGAAGATC+TGG 0.294522 6:+53165296 None:intergenic
CTGCACTTTCGAGTTGGACT+TGG 0.299490 6:+53165025 None:intergenic
AGCCACAGACTCTCCTTCTC+TGG 0.311063 6:+53165374 None:intergenic
TCAATATTTGAAGTTGTTAC+TGG 0.317605 6:-53165107 MsaT032466.1:CDS
GTATCAATCTCCTCGTCTTT+AGG 0.352964 6:+53164957 None:intergenic
AGATCTTCCCGAGCCTATGC+TGG 0.365711 6:-53165294 MsaT032466.1:CDS
TCTTTATCTTCAAGCCATAA+AGG 0.388538 6:-53165056 MsaT032466.1:CDS
AGTTAAGCAGACTAAGGCTT+TGG 0.403722 6:-53164985 MsaT032466.1:CDS
TGCTGGGATTTAACTTTGAA+AGG 0.416022 6:-53165277 MsaT032466.1:CDS
ATTGCCATGGCACACTACTT+TGG 0.419300 6:-53165194 MsaT032466.1:CDS
TCTCCTTCTCTGGGTCAGAT+TGG 0.447533 6:+53165384 None:intergenic
CTCTTGTTGTTATTGCATGA+TGG 0.451557 6:+53164395 None:intergenic
TCGATATGCTTAGCCCTAGC+AGG 0.457819 6:+53164429 None:intergenic
GCATAGGCTCGGGAAGATCT+GGG 0.458847 6:+53165297 None:intergenic
CAGTTTCCCTAATGAGCAAT+GGG 0.462686 6:-53165348 MsaT032466.1:CDS
GATTTCAACGGTCGTCGTGC+TGG 0.463510 6:-53165449 MsaT032466.1:CDS
GAGGAAGATGAAGATGAAGA+AGG 0.473888 6:-53164558 MsaT032466.1:CDS
ATCGCATGCAAGAGAAAGAT+TGG 0.476387 6:-53165253 MsaT032466.1:CDS
GCTGGGATTTAACTTTGAAA+GGG 0.477875 6:-53165276 MsaT032466.1:CDS
ATCACTATGAACAGCAACTA+AGG 0.486624 6:+53165226 None:intergenic
AATATGACCCTGATAAATTC+TGG 0.489252 6:-53164506 MsaT032466.1:CDS
CACAGTACGGCTCACATCCC+TGG 0.489652 6:+53164931 None:intergenic
ATCTCAAGTTAAGCAGACTA+AGG 0.494523 6:-53164991 MsaT032466.1:CDS
TATCATTTATCATATTGAAA+AGG 0.496927 6:+53165135 None:intergenic
AGCCCGCTGCACTTTCGAGT+TGG 0.497770 6:+53165019 None:intergenic
GTGTGAATATCACCCCTGCT+AGG 0.500091 6:-53164443 MsaT032466.1:CDS
AAAAGTTCTTCCATCTCTGA+TGG 0.502456 6:+53164582 None:intergenic
CACTACTTTGGGGCAAGATG+TGG 0.517023 6:-53165182 MsaT032466.1:CDS
CGAGGAGATTGATACGGACC+AGG 0.523355 6:-53164949 MsaT032466.1:CDS
GTTAAATCCCAGCATAGGCT+CGG 0.523411 6:+53165286 None:intergenic
GTCCAACTCGAAAGTGCAGC+GGG 0.524882 6:-53165021 MsaT032466.1:CDS
TCATGGCAAAACTTGTCAGC+AGG 0.530511 6:-53165712 MsaT032466.1:intron
TCGTCGTGCTGGTCTCATCA+AGG 0.541056 6:-53165438 MsaT032466.1:CDS
TTTGTGAGAGGTGGTTCCAT+GGG 0.547613 6:-53164470 MsaT032466.1:CDS
TCGGTGATATTTGTGAGAGG+TGG 0.556309 6:-53164479 MsaT032466.1:CDS
GCCACAGACTCTCCTTCTCT+GGG 0.561677 6:+53165375 None:intergenic
TCAAAGTTAAATCCCAGCAT+AGG 0.563679 6:+53165281 None:intergenic
GATCTTCCCGAGCCTATGCT+GGG 0.565149 6:-53165293 MsaT032466.1:CDS
TTAAATCCCAGCATAGGCTC+GGG 0.565590 6:+53165287 None:intergenic
TGTGAATATCACCCCTGCTA+GGG 0.567860 6:-53164442 MsaT032466.1:CDS
ACAGTTTCCCTAATGAGCAA+TGG 0.568382 6:-53165349 MsaT032466.1:CDS
TGCAGAAAAGAAACAAGTAA+AGG 0.572021 6:-53165084 MsaT032466.1:CDS
CTACACAGGCCCATCAGAGA+TGG 0.573864 6:-53164592 MsaT032466.1:intron
GTAACAACTTCAAATATTGA+CGG 0.577921 6:+53165110 None:intergenic
TGATATTCACACATTTCCCA+TGG 0.578749 6:+53164454 None:intergenic
CGATATGCTTAGCCCTAGCA+GGG 0.578888 6:+53164430 None:intergenic
CATAGGCTCGGGAAGATCTG+GGG 0.581002 6:+53165298 None:intergenic
ATTTGTGAGAGGTGGTTCCA+TGG 0.592358 6:-53164471 MsaT032466.1:CDS
AAAGTTCTTCCATCTCTGAT+GGG 0.593450 6:+53164583 None:intergenic
TGCCATGGCACACTACTTTG+GGG 0.603166 6:-53165192 MsaT032466.1:CDS
TAAAGACGAGGAGATTGATA+CGG 0.606103 6:-53164955 MsaT032466.1:CDS
TTGCTGTTCATAGTGATGCA+TGG 0.606640 6:-53165220 MsaT032466.1:CDS
ATAGGCTCGGGAAGATCTGG+GGG 0.607426 6:+53165299 None:intergenic
CGAAAATCCAGAATTTATCA+GGG 0.613625 6:+53164499 None:intergenic
TGCCCCAAAGTAGTGTGCCA+TGG 0.618279 6:+53165190 None:intergenic
TAACCAATCTGACCCAGAGA+AGG 0.618594 6:-53165387 MsaT032466.1:CDS
TGATGCATGGTTAATTGCCA+TGG 0.631616 6:-53165207 MsaT032466.1:CDS
ACCCAGAGAAGGAGAGTCTG+TGG 0.633591 6:-53165376 MsaT032466.1:CDS
ATAGAACTCTTCAACATCAG+TGG 0.635056 6:+53165409 None:intergenic
GAAGAAGGAGAGAACTCATG+TGG 0.669374 6:-53164543 MsaT032466.1:CDS
GAGGAGATTGATACGGACCA+GGG 0.672430 6:-53164948 MsaT032466.1:CDS
AGTCCAACTCGAAAGTGCAG+CGG 0.678881 6:-53165022 MsaT032466.1:CDS
TTTGGAGTTACCTAAAGACG+AGG 0.685384 6:-53164967 MsaT032466.1:CDS
GAGAACTCATGTGGTGCATG+CGG 0.697071 6:-53164534 MsaT032466.1:CDS
GATATGCTTAGCCCTAGCAG+GGG 0.740274 6:+53164431 None:intergenic
GTGGATTTGACAAATCTGAG+AGG 0.764132 6:-53165163 MsaT032466.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 53164404 53165732 53164404 ID=MsaG032466
Chr6 mRNA 53164404 53165732 53164404 ID=MsaT032466.1;Parent=MsaG032466
Chr6 exon 53164404 53164606 53164404 ID=MsaT032466.1.exon3;Parent=MsaT032466.1
Chr6 CDS 53164404 53164606 53164404 ID=cds.MsaT032466.1;Parent=MsaT032466.1
Chr6 exon 53164946 53165472 53164946 ID=MsaT032466.1.exon2;Parent=MsaT032466.1
Chr6 CDS 53164946 53165472 53164946 ID=cds.MsaT032466.1;Parent=MsaT032466.1
Chr6 exon 53165713 53165732 53165713 ID=MsaT032466.1.exon1;Parent=MsaT032466.1
Chr6 CDS 53165713 53165732 53165713 ID=cds.MsaT032466.1;Parent=MsaT032466.1
Gene Sequence

>MsaT032466.1

ATGGCAAAACTTGTCAGCAGAGATTTCAACGGTCGTCGTGCTGGTCTCATCAAGGCTCTCACCACTGATGTTGAAGAGTTCTATAACCAATCTGACCCAGAGAAGGAGAGTCTGTGGCTATACAGTTTCCCTAATGAGCAATGGGAAGTCAATTTACCTTCTATGGAAGTTCCCCCAGATCTTCCCGAGCCTATGCTGGGATTTAACTTTGAAAGGGATCGCATGCAAGAGAAAGATTGGCTATCCTTAGTTGCTGTTCATAGTGATGCATGGTTAATTGCCATGGCACACTACTTTGGGGCAAGATGTGGATTTGACAAATCTGAGAGGATGCGCCTTTTCAATATGATAAATGATATGCCGTCAATATTTGAAGTTGTTACTGGTGCAGAAAAGAAACAAGTAAAGGAGAAGTCTTTATCTTCAAGCCATAAAGGCAGCAAATCCAAGTCCAACTCGAAAGTGCAGCGGGCTATAAAATCTCAAGTTAAGCAGACTAAGGCTTTGGAGTTACCTAAAGACGAGGAGATTGATACGGACCAGGGATGCCCATCAGAGATGGAAGAACTTTTTGAGGAAGATGAAGATGAAGAAGGAGAGAACTCATGTGGTGCATGCGGTGAGCAATATGACCCTGATAAATTCTGGATTTTCGGTGATATTTGTGAGAGGTGGTTCCATGGGAAATGTGTGAATATCACCCCTGCTAGGGCTAAGCATATCGATATATACAAGTCCATCATGCAATAA

Protein sequence

>MsaT032466.1

MAKLVSRDFNGRRAGLIKALTTDVEEFYNQSDPEKESLWLYSFPNEQWEVNLPSMEVPPDLPEPMLGFNFERDRMQEKDWLSLVAVHSDAWLIAMAHYFGARCGFDKSERMRLFNMINDMPSIFEVVTGAEKKQVKEKSLSSSHKGSKSKSNSKVQRAIKSQVKQTKALELPKDEEIDTDQGCPSEMEELFEEDEDEEGENSCGACGEQYDPDKFWIFGDICERWFHGKCVNITPARAKHIDIYKSIMQ*