AlfalfaGEDB | MsaG032470

M. sativa ssp. caerulea / MsaG032470

KEGG: -
GO: -
PFAM: PF12165.11

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032470	KAF7843566.1	70.800	250	62	2	35	284	6	244	1.92e-105	317

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032470	sp\|Q5XEM9\|ALFL5_ARATH	58.400	250	97	2	35	284	7	249	8.88e-87	263

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032470	tr\|A0A835CHP9\|A0A835CHP9_9FABA	70.800	250	62	2	35	284	6	244	9.17e-106	317

TFs/TRs:

Gene ID	Type	Classification
MsaG032470	TR	PHD

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG001038	MsaG032470	0.809022	3.962192e-50	6.608588e-48
MsaG001300	MsaG032470	0.810261	2.152710e-50	3.699689e-48
MsaG001486	MsaG032470	0.802109	1.100645e-48	1.560845e-46
MsaG001739	MsaG032470	0.820487	1.171970e-52	2.611074e-50
MsaG003682	MsaG032470	0.809931	2.534077e-50	4.320174e-48
MsaG003974	MsaG032470	0.800510	2.330764e-48	3.187778e-46
MsaG004030	MsaG032470	0.812106	8.604572e-51	1.547076e-48
MsaG005995	MsaG032470	0.802436	9.432467e-49	1.347740e-46
MsaG006521	MsaG032470	0.806180	1.579295e-49	2.461363e-47
MsaG006920	MsaG032470	0.803706	5.168044e-49	7.603519e-47
MsaG007357	MsaG032470	0.806172	1.585171e-49	2.470067e-47
MsaG008010	MsaG032470	0.819103	2.418640e-52	5.195458e-50
MsaG009654	MsaG032470	0.807588	7.985253e-50	1.286648e-47
MsaG010498	MsaG032470	0.807029	1.047333e-49	1.665302e-47
MsaG016370	MsaG032470	0.804327	3.843962e-49	5.737471e-47
MsaG017092	MsaG032470	0.823807	2.007713e-53	4.889042e-51
MsaG017683	MsaG032470	0.821021	8.845258e-53	1.998774e-50
MsaG018011	MsaG032470	0.813222	4.918563e-51	9.092859e-49
MsaG018524	MsaG032470	0.801297	1.612439e-48	2.244888e-46
MsaG019758	MsaG032470	0.800270	2.606992e-48	3.546279e-46
MsaG020532	MsaG032470	0.801966	1.177416e-48	1.664334e-46
MsaG023676	MsaG032470	0.814206	2.993829e-51	5.672884e-49
MsaG024365	MsaG032470	0.816088	1.148557e-51	2.282299e-49
MsaG028251	MsaG032470	0.807566	8.070090e-50	1.299645e-47
MsaG028738	MsaG032470	0.811801	1.002187e-50	1.788511e-48
MsaG028644	MsaG032470	0.813286	4.762708e-51	8.818461e-49
MsaG029161	MsaG032470	0.804571	3.421141e-49	5.135210e-47
MsaG032072	MsaG032470	0.803075	6.973362e-49	1.011096e-46
MsaG032426	MsaG032470	0.805526	2.164238e-49	3.321364e-47
MsaG032470	MsaG033310	0.813004	5.487640e-51	1.008981e-48
MsaG032470	MsaG033756	0.803333	6.168664e-49	8.997639e-47
MsaG032470	MsaG034745	0.819574	1.891299e-52	4.112861e-50
MsaG032470	MsaG035414	0.806185	1.575427e-49	2.455646e-47
MsaG032470	MsaG039843	0.807471	8.452496e-50	1.358167e-47
MsaG032470	MsaG040099	0.816338	1.010385e-51	2.020634e-49
MsaG032470	MsaG040355	0.827700	2.417151e-54	6.554687e-52
MsaG032470	MsaG042686	0.810847	1.610326e-50	2.807392e-48
MsaG032470	MsaG044018	0.845178	8.957624e-59	4.128386e-56
MsaG032470	MsaG045267	0.813038	5.395059e-51	9.928064e-49
MsaG032470	MsaG046321	0.803808	4.921191e-49	7.257481e-47
MsaG032470	MsaG046537	0.813325	4.668688e-51	8.653063e-49
MsaG032470	MsaG047188	0.802607	8.699479e-49	1.247912e-46
MsaG032470	MsaG008684	0.804495	3.546432e-49	5.314000e-47
MsaG032470	MsaG017508	0.815378	1.650493e-51	3.221267e-49
MsaG032470	MsaG013409	0.820187	1.372262e-52	3.033223e-50
MsaG032470	MsaG019851	0.813591	4.084061e-51	7.620352e-49
MsaG032470	MsaG027456	0.812215	8.147027e-51	1.468785e-48
MsaG032470	MsaG026953	0.822032	5.180267e-53	1.202377e-50
MsaG032470	MsaG034467	0.802539	8.986638e-49	1.287086e-46
MsaG032470	MsaG033619	0.822998	3.096270e-53	7.376228e-51
MsaG032470	MsaG031702	0.801336	1.583221e-48	2.206203e-46
MsaG032470	MsaG036600	0.816176	1.097819e-51	2.186439e-49
MsaG032470	MsaG036499	0.800326	2.539810e-48	3.459246e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032470	MtrunA17_Chr8g0343441	73.600	250	55	2	35	284	6	244	3.87e-106	309
MsaG032470	MtrunA17_Chr2g0300241	59.761	251	81	3	36	284	10	242	3.42e-87	260
MsaG032470	MtrunA17_Chr2g0329821	60.976	246	84	2	39	284	6	239	7.18e-87	259
MsaG032470	MtrunA17_Chr1g0150821	62.651	249	89	2	36	284	8	252	4.58e-86	258
MsaG032470	MtrunA17_Chr4g0076771	56.327	245	95	2	40	284	12	244	1.41e-81	246
MsaG032470	MtrunA17_Chr4g0006381	52.419	248	92	8	39	284	8	231	1.24e-67	210
MsaG032470	MtrunA17_Chr7g0242511	48.374	246	105	6	39	284	8	231	5.76e-65	203

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032470	AT5G20510.1	58.400	250	97	2	35	284	7	249	9.03e-88	263
MsaG032470	AT3G42790.1	59.036	249	85	4	36	284	8	239	8.95e-86	257
MsaG032470	AT1G14510.1	58.367	245	87	2	40	284	11	240	4.73e-80	243
MsaG032470	AT2G02470.1	58.367	245	92	3	40	284	11	245	3.26e-77	236
MsaG032470	AT2G02470.2	58.367	245	83	4	40	284	11	236	1.10e-75	231
MsaG032470	AT5G26210.1	62.400	250	83	2	36	285	7	245	1.91e-74	228
MsaG032470	AT5G05610.2	52.823	248	93	5	37	284	7	230	5.97e-74	227
MsaG032470	AT5G05610.1	52.823	248	93	5	37	284	7	230	5.97e-74	227
MsaG032470	AT3G11200.1	52.800	250	93	6	37	284	9	235	1.11e-66	208
MsaG032470	AT5G20510.2	55.660	212	87	2	73	284	4	208	7.59e-66	205
MsaG032470	AT5G20510.3	65.000	160	56	0	35	194	7	166	7.89e-66	204
MsaG032470	AT2G02470.3	67.333	150	46	1	40	189	11	157	2.27e-56	182
MsaG032470	AT3G11200.2	52.830	212	79	6	73	284	32	222	4.49e-54	176

Find 80 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTTGGATTTGCTGCCTTTA+TGG	0.143405	6:+53228836	None:intergenic
GGAAGTCAATTTACCTTCTA+TGG	0.184392	6:-53229121	MsaT032470.1:CDS
CCGAAAATCCAGAATTTATC+AGG	0.216776	6:+53228292	None:intergenic
TTGACTTCCCATTGCTCATT+AGG	0.233040	6:+53229135	None:intergenic
TTGCCATGGCACACTACTTT+GGG	0.283032	6:-53228987	MsaT032470.1:CDS
TGACTTCCCATTGCTCATTA+GGG	0.286910	6:+53229136	None:intergenic
AGCATAGGCTCGGGAAGATC+TGG	0.294522	6:+53229090	None:intergenic
CTTCACAGCGAATCAATGTT+AGG	0.296552	6:-53229573	MsaT032470.1:CDS
CTGCACTTTCGAGTTGGACT+TGG	0.299490	6:+53228819	None:intergenic
AGCCACAGACTCTCCTTCTC+TGG	0.311063	6:+53229168	None:intergenic
TCAATATTTGAAGTTGTTAC+TGG	0.317605	6:-53228901	MsaT032470.1:CDS
GTATCAATCTCCTCGTCTTT+AGG	0.352964	6:+53228751	None:intergenic
AGATCTTCCCGAGCCTATGC+TGG	0.365711	6:-53229088	MsaT032470.1:CDS
TCTTTATCTTCAAGCCATAA+AGG	0.388538	6:-53228850	MsaT032470.1:CDS
AGTTAAGCAGACTAAGGCTT+TGG	0.403722	6:-53228779	MsaT032470.1:CDS
TGCTGGGATTTAACTTTGAA+AGG	0.416022	6:-53229071	MsaT032470.1:CDS
ATTGCCATGGCACACTACTT+TGG	0.419300	6:-53228988	MsaT032470.1:CDS
TCTCCTTCTCTGGGTCAGAT+TGG	0.447533	6:+53229178	None:intergenic
CTCTTGTTGTTATTGCATGA+TGG	0.451557	6:+53228189	None:intergenic
TTCACAGCGAATCAATGTTA+GGG	0.452573	6:-53229572	MsaT032470.1:CDS
TCGATATGCTTAGCCCTAGC+AGG	0.457819	6:+53228223	None:intergenic
GCATAGGCTCGGGAAGATCT+GGG	0.458847	6:+53229091	None:intergenic
CAGTTTCCCTAATGAGCAAT+GGG	0.462686	6:-53229142	MsaT032470.1:CDS
GATTTCAACGGTCGTCGTGC+TGG	0.463510	6:-53229243	MsaT032470.1:CDS
ATTCGCTGTGAAGGAAAAGA+AGG	0.468130	6:+53229583	None:intergenic
GAGGAAGATGAAGATGAAGA+AGG	0.473888	6:-53228352	MsaT032470.1:CDS
ATCGCATGCAAGAGAAAGAT+TGG	0.476387	6:-53229047	MsaT032470.1:CDS
GCTGGGATTTAACTTTGAAA+GGG	0.477875	6:-53229070	MsaT032470.1:CDS
ATCACTATGAACAGCAACTA+AGG	0.486624	6:+53229020	None:intergenic
AATATGACCCTGATAAATTC+TGG	0.489252	6:-53228300	MsaT032470.1:CDS
CACAGTACGGCTCACATCCC+TGG	0.489652	6:+53228725	None:intergenic
ATCTCAAGTTAAGCAGACTA+AGG	0.494523	6:-53228785	MsaT032470.1:CDS
TATCATTTATCATATTGAAA+AGG	0.496927	6:+53228929	None:intergenic
AGCCCGCTGCACTTTCGAGT+TGG	0.497770	6:+53228813	None:intergenic
GTGTGAATATCACCCCTGCT+AGG	0.500091	6:-53228237	MsaT032470.1:CDS
AAAAGTTCTTCCATCTCTGA+TGG	0.502456	6:+53228376	None:intergenic
CACTACTTTGGGGCAAGATG+TGG	0.517023	6:-53228976	MsaT032470.1:CDS
CGAGGAGATTGATACGGACC+AGG	0.523355	6:-53228743	MsaT032470.1:CDS
GTTAAATCCCAGCATAGGCT+CGG	0.523411	6:+53229080	None:intergenic
GTCCAACTCGAAAGTGCAGC+GGG	0.524882	6:-53228815	MsaT032470.1:CDS
TCATGGCAAAACTTGTCAGC+AGG	0.530511	6:-53229506	MsaT032470.1:intron
TCGTCGTGCTGGTCTCATCA+AGG	0.541056	6:-53229232	MsaT032470.1:CDS
TTTGTGAGAGGTGGTTCCAT+GGG	0.547613	6:-53228264	MsaT032470.1:CDS
AAATCCCGTGGAATTCGAGT+TGG	0.554000	6:-53229544	MsaT032470.1:CDS
TCGGTGATATTTGTGAGAGG+TGG	0.556309	6:-53228273	MsaT032470.1:CDS
GCCACAGACTCTCCTTCTCT+GGG	0.561677	6:+53229169	None:intergenic
TCAAAGTTAAATCCCAGCAT+AGG	0.563679	6:+53229075	None:intergenic
GATCTTCCCGAGCCTATGCT+GGG	0.565149	6:-53229087	MsaT032470.1:CDS
TTAAATCCCAGCATAGGCTC+GGG	0.565590	6:+53229081	None:intergenic
TGTGAATATCACCCCTGCTA+GGG	0.567860	6:-53228236	MsaT032470.1:CDS
ACAGTTTCCCTAATGAGCAA+TGG	0.568382	6:-53229143	MsaT032470.1:CDS
ATCTCTGAAAACTTCTTCGA+CGG	0.569114	6:+53229263	None:intergenic
CTAACATTGATTCGCTGTGA+AGG	0.570338	6:+53229574	None:intergenic
TGCAGAAAAGAAACAAGTAA+AGG	0.572021	6:-53228878	MsaT032470.1:CDS
CTACACAGGCCCATCAGAGA+TGG	0.573864	6:-53228386	MsaT032470.1:intron
GTAACAACTTCAAATATTGA+CGG	0.577921	6:+53228904	None:intergenic
TGATATTCACACATTTCCCA+TGG	0.578749	6:+53228248	None:intergenic
CGATATGCTTAGCCCTAGCA+GGG	0.578888	6:+53228224	None:intergenic
CATAGGCTCGGGAAGATCTG+GGG	0.581002	6:+53229092	None:intergenic
ATTTGTGAGAGGTGGTTCCA+TGG	0.592358	6:-53228265	MsaT032470.1:CDS
AAAGTTCTTCCATCTCTGAT+GGG	0.593450	6:+53228377	None:intergenic
TGCCATGGCACACTACTTTG+GGG	0.603166	6:-53228986	MsaT032470.1:CDS
TAAAGACGAGGAGATTGATA+CGG	0.606103	6:-53228749	MsaT032470.1:CDS
TTGCTGTTCATAGTGATGCA+TGG	0.606640	6:-53229014	MsaT032470.1:CDS
ATAGGCTCGGGAAGATCTGG+GGG	0.607426	6:+53229093	None:intergenic
CGAAAATCCAGAATTTATCA+GGG	0.613625	6:+53228293	None:intergenic
TCACAGCGAATCAATGTTAG+GGG	0.615098	6:-53229571	MsaT032470.1:CDS
TGCCCCAAAGTAGTGTGCCA+TGG	0.618279	6:+53228984	None:intergenic
TAACCAATCTGACCCAGAGA+AGG	0.618594	6:-53229181	MsaT032470.1:CDS
TGATGCATGGTTAATTGCCA+TGG	0.631616	6:-53229001	MsaT032470.1:CDS
ACCCAGAGAAGGAGAGTCTG+TGG	0.633591	6:-53229170	MsaT032470.1:CDS
ATAGAACTCTTCAACATCAG+TGG	0.635056	6:+53229203	None:intergenic
GTTAGGGGAAAGAAATCCCG+TGG	0.660372	6:-53229556	MsaT032470.1:CDS
GAAGAAGGAGAGAACTCATG+TGG	0.669374	6:-53228337	MsaT032470.1:CDS
GAGGAGATTGATACGGACCA+GGG	0.672430	6:-53228742	MsaT032470.1:CDS
AGTCCAACTCGAAAGTGCAG+CGG	0.678881	6:-53228816	MsaT032470.1:CDS
TTTGGAGTTACCTAAAGACG+AGG	0.685384	6:-53228761	MsaT032470.1:CDS
GAGAACTCATGTGGTGCATG+CGG	0.697071	6:-53228328	MsaT032470.1:CDS
GATATGCTTAGCCCTAGCAG+GGG	0.740274	6:+53228225	None:intergenic
GTGGATTTGACAAATCTGAG+AGG	0.764132	6:-53228957	MsaT032470.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	53228198	53229611	53228198	ID=MsaG032470
Chr6	mRNA	53228198	53229611	53228198	ID=MsaT032470.1;Parent=MsaG032470
Chr6	exon	53228198	53228400	53228198	ID=MsaT032470.1.exon3;Parent=MsaT032470.1
Chr6	CDS	53228198	53228400	53228198	ID=cds.MsaT032470.1;Parent=MsaT032470.1
Chr6	exon	53228740	53229298	53228740	ID=MsaT032470.1.exon2;Parent=MsaT032470.1
Chr6	CDS	53228740	53229298	53228740	ID=cds.MsaT032470.1;Parent=MsaT032470.1
Chr6	exon	53229507	53229611	53229507	ID=MsaT032470.1.exon1;Parent=MsaT032470.1
Chr6	CDS	53229507	53229611	53229507	ID=cds.MsaT032470.1;Parent=MsaT032470.1

Gene Sequence

>MsaT032470.1

ATGAAACCTTCTTTTCCTTCACAGCGAATCAATGTTAGGGGAAAGAAATCCCGTGGAATTCGAGTTGGAAAAAAAACTCGTGTTCATGGCAAAACTTGTCAGCAGTATAATCTACACACCGTCGAAGAAGTTTTCAGAGATTTCAACGGTCGTCGTGCTGGTCTCATCAAGGCTCTCACCACTGATGTTGAAGAGTTCTATAACCAATCTGACCCAGAGAAGGAGAGTCTGTGGCTATACAGTTTCCCTAATGAGCAATGGGAAGTCAATTTACCTTCTATGGAAGTTCCCCCAGATCTTCCCGAGCCTATGCTGGGATTTAACTTTGAAAGGGATCGCATGCAAGAGAAAGATTGGCTATCCTTAGTTGCTGTTCATAGTGATGCATGGTTAATTGCCATGGCACACTACTTTGGGGCAAGATGTGGATTTGACAAATCTGAGAGGATGCGCCTTTTCAATATGATAAATGATATGCCGTCAATATTTGAAGTTGTTACTGGTGCAGAAAAGAAACAAGTAAAGGAGAAGTCTTTATCTTCAAGCCATAAAGGCAGCAAATCCAAGTCCAACTCGAAAGTGCAGCGGGCTATAAAATCTCAAGTTAAGCAGACTAAGGCTTTGGAGTTACCTAAAGACGAGGAGATTGATACGGACCAGGGATGCCCATCAGAGATGGAAGAACTTTTTGAGGAAGATGAAGATGAAGAAGGAGAGAACTCATGTGGTGCATGCGGTGAGCAATATGACCCTGATAAATTCTGGATTTTCGGTGATATTTGTGAGAGGTGGTTCCATGGGAAATGTGTGAATATCACCCCTGCTAGGGCTAAGCATATCGATATATACAAGTCCATCATGCAATAA

Protein sequence

>MsaT032470.1

MKPSFPSQRINVRGKKSRGIRVGKKTRVHGKTCQQYNLHTVEEVFRDFNGRRAGLIKALTTDVEEFYNQSDPEKESLWLYSFPNEQWEVNLPSMEVPPDLPEPMLGFNFERDRMQEKDWLSLVAVHSDAWLIAMAHYFGARCGFDKSERMRLFNMINDMPSIFEVVTGAEKKQVKEKSLSSSHKGSKSKSNSKVQRAIKSQVKQTKALELPKDEEIDTDQGCPSEMEELFEEDEDEEGENSCGACGEQYDPDKFWIFGDICERWFHGKCVNITPARAKHIDIYKSIMQ*