AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG032472


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032472 MtrunA17_Chr8g0343441 72.340 47 13 0 13 59 198 244 4.37e-19 77.0
MsaG032472 MtrunA17_Chr2g0329821 68.085 47 15 0 13 59 193 239 7.20e-18 73.9
MsaG032472 MtrunA17_Chr1g0150821 72.093 43 12 0 17 59 210 252 2.93e-17 72.4
MsaG032472 MtrunA17_Chr4g0076771 65.909 44 15 0 16 59 201 244 9.21e-17 70.9
MsaG032472 MtrunA17_Chr7g0242511 65.116 43 15 0 17 59 189 231 2.35e-16 69.7
MsaG032472 MtrunA17_Chr4g0006381 65.116 43 15 0 17 59 189 231 3.05e-16 69.3
MsaG032472 MtrunA17_Chr2g0300241 62.500 48 16 1 14 59 195 242 6.16e-15 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG032472 AT5G20510.2 65.957 47 16 0 13 59 162 208 1.34e-17 72.8
MsaG032472 AT5G20510.1 65.957 47 16 0 13 59 203 249 2.47e-17 72.8
MsaG032472 AT5G26210.1 68.889 45 14 0 15 59 200 244 6.06e-17 71.6
MsaG032472 AT3G42790.1 65.957 47 16 0 13 59 193 239 6.67e-17 71.6
MsaG032472 AT5G05610.2 59.574 47 19 0 13 59 184 230 3.04e-16 69.7
MsaG032472 AT5G05610.1 59.574 47 19 0 13 59 184 230 3.04e-16 69.7
MsaG032472 AT2G02470.1 67.442 43 14 0 17 59 203 245 6.44e-16 68.9
MsaG032472 AT2G02470.2 67.442 43 14 0 17 59 194 236 7.31e-16 68.9
MsaG032472 AT1G14510.1 67.442 43 14 0 17 59 198 240 1.51e-15 68.2
MsaG032472 AT3G11200.2 57.447 47 20 0 13 59 176 222 1.92e-15 67.4
MsaG032472 AT3G11200.1 57.447 47 20 0 13 59 189 235 3.52e-15 67.0

Find 16 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGAAAATCCAGAATTTATC+AGG 0.249988 6:+53284160 None:intergenic
CTCTTGTTGTTATTGCATGA+TGG 0.451557 6:+53284057 None:intergenic
TCGATATGCTTAGCCCTAGC+AGG 0.457819 6:+53284091 None:intergenic
AATATGACCCTGATAAATTC+TGG 0.489252 6:-53284168 MsaT032472.1:CDS
GTGTGAATATCACCCCTGCT+AGG 0.500091 6:-53284105 MsaT032472.1:CDS
GAGGAAGATGAAGATGAAGA+CGG 0.529823 6:-53284220 MsaT032472.1:CDS
TCGGTGACATTTGTGAGAGG+TGG 0.533723 6:-53284141 MsaT032472.1:CDS
TTTGTGAGAGGTGGTTCCAT+GGG 0.542875 6:-53284132 MsaT032472.1:CDS
TGTGAATATCACCCCTGCTA+GGG 0.567860 6:-53284104 MsaT032472.1:CDS
TGATATTCACACATTTCCCA+TGG 0.578749 6:+53284116 None:intergenic
CGATATGCTTAGCCCTAGCA+GGG 0.578888 6:+53284092 None:intergenic
ATTTGTGAGAGGTGGTTCCA+TGG 0.579292 6:-53284133 MsaT032472.1:CDS
GAGAACTCATGCGGTGCATG+CGG 0.601432 6:-53284196 MsaT032472.1:CDS
CGAAAATCCAGAATTTATCA+GGG 0.613625 6:+53284161 None:intergenic
GAAGACGGAGAGAACTCATG+CGG 0.708786 6:-53284205 MsaT032472.1:CDS
GATATGCTTAGCCCTAGCAG+GGG 0.740274 6:+53284093 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr6 gene 53284066 53284257 53284066 ID=MsaG032472
Chr6 mRNA 53284066 53284257 53284066 ID=MsaT032472.1;Parent=MsaG032472
Chr6 exon 53284066 53284257 53284066 ID=MsaT032472.1.exon1;Parent=MsaT032472.1
Chr6 CDS 53284066 53284257 53284066 ID=cds.MsaT032472.1;Parent=MsaT032472.1
Gene Sequence

>MsaT032472.1

ATGGAAGAACTTTTTGAGGAAGATGAAGATGAAGACGGAGAGAACTCATGCGGTGCATGCGGTGAGCAATATGACCCTGATAAATTCTGGATTTTCGGTGACATTTGTGAGAGGTGGTTCCATGGGAAATGTGTGAATATCACCCCTGCTAGGGCTAAGCATATCGATATATACAAGTCCATCATGCAATAA

Protein sequence

>MsaT032472.1

MEELFEEDEDEDGENSCGACGEQYDPDKFWIFGDICERWFHGKCVNITPARAKHIDIYKSIMQ*