AlfalfaGEDB | MsaG032478

M. sativa ssp. caerulea / MsaG032478

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032478	RHN43614.1	93.662	142	9	0	1	142	1	142	2.26e-89	269

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032478	tr\|A0A396GVU6\|A0A396GVU6_MEDTR	93.662	142	9	0	1	142	1	142	1.08e-89	269

TFs/TRs:

Gene ID	Type	Classification
MsaG032478	TF	FAR1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG032478	MtrunA17_Chr8g0389811	93.662	142	9	0	1	142	1	142	2.08e-93	269
MsaG032478	MtrunA17_Chr6g0473421	55.263	76	34	0	63	138	34	109	3.27e-28	102
MsaG032478	MtrunA17_Chr8g0366311	49.565	115	53	2	1	115	1	110	2.89e-26	95.9
MsaG032478	MtrunA17_Chr1g0203051	40.909	132	73	2	8	139	8	134	3.99e-26	102
MsaG032478	MtrunA17_Chr1g0172931	52.941	85	40	0	55	139	47	131	3.14e-25	94.4
MsaG032478	MtrunA17_Chr2g0331371	52.632	76	36	0	63	138	34	109	1.15e-20	84.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 18 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATAGTTTGTCGTTGTCTA+AGG	0.298086	6:+53438897	MsaT032478.1:CDS
AATTAAGTTCAGAAGATGTT+AGG	0.343441	6:+53439076	MsaT032478.1:CDS
CTACAGCAACATCTACAAAA+TGG	0.362119	6:-53439104	None:intergenic
ATCATTTCTACTGCCTTCAA+TGG	0.411817	6:-53438977	None:intergenic
GTCTTATGCTGTGAATTGTT+TGG	0.435978	6:+53439026	MsaT032478.1:CDS
CTGCCTTCAATGGTTTCCTC+AGG	0.455452	6:-53438967	None:intergenic
AATTGGTATGCTAGCTGTCA+TGG	0.466201	6:+53439141	MsaT032478.1:CDS
ATTTATCATGTTTATTGTGG+TGG	0.466786	6:+53439256	MsaT032478.1:CDS
AGGATTTATCATGTTTATTG+TGG	0.521924	6:+53439253	MsaT032478.1:CDS
ATTGAAGGCAGTAGAAATGA+TGG	0.562841	6:+53438979	MsaT032478.1:CDS
CACTTGTTTGTTATCGACAA+GGG	0.565505	6:+53439223	MsaT032478.1:CDS
ATACCTGAGGAAACCATTGA+AGG	0.571518	6:+53438964	MsaT032478.1:CDS
AAGGTAATGAGAAGCAAAAG+TGG	0.580541	6:+53439183	MsaT032478.1:CDS
ATGATGGTGACAACATATGT+AGG	0.587276	6:+53438995	MsaT032478.1:CDS
TGATGAAGAGGATTCGACAC+AGG	0.605639	6:+53438789	MsaT032478.1:CDS
ACACTTGTTTGTTATCGACA+AGG	0.628293	6:+53439222	MsaT032478.1:CDS
GAATGAATCTGAAATACCTG+AGG	0.681684	6:+53438951	MsaT032478.1:CDS
TTATCGACAAGGGTTTACAG+AGG	0.687644	6:+53439233	MsaT032478.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	53438760	53439299	53438760	ID=MsaG032478
Chr6	mRNA	53438760	53439299	53438760	ID=MsaT032478.1;Parent=MsaG032478
Chr6	exon	53438760	53438810	53438760	ID=MsaT032478.1.exon1;Parent=MsaT032478.1
Chr6	CDS	53438760	53438810	53438760	ID=cds.MsaT032478.1;Parent=MsaT032478.1
Chr6	exon	53438892	53439299	53438892	ID=MsaT032478.1.exon2;Parent=MsaT032478.1
Chr6	CDS	53438892	53439299	53438892	ID=cds.MsaT032478.1;Parent=MsaT032478.1

Gene Sequence

>MsaT032478.1

ATGGTGGATTTTCAATATGATTCGACTTTTGATGAAGAGGATTCGACACAGGGATTGGATAGTTTGTCGTTGTCTAAGGATGATTCTGATCGTGCAGATGTTATTAACGAGAATGAATCTGAAATACCTGAGGAAACCATTGAAGGCAGTAGAAATGATGGTGACAACATATGTAGGTTCGACGAGTCTTATGCTGTGAATTGTTTGGAAGACATTTCAAGTATTAACTTCAAAGAATTAAGTTCAGAAGATGTTAGGAGATACCATTTTGTAGATGTTGCTGTAGCATTTACTTTTTATAATTGGTATGCTAGCTGTCATGGTTTTGCTGCAAGAAAAAGCAAGGTAATGAGAAGCAAAAGTGGTGAACTAACTCAACAGACACTTGTTTGTTATCGACAAGGGTTTACAGAGGATTTATCATGTTTATTGTGGTGGATTTATTATTTCTATATTTGA

Protein sequence

>MsaT032478.1

MVDFQYDSTFDEEDSTQGLDSLSLSKDDSDRADVINENESEIPEETIEGSRNDGDNICRFDESYAVNCLEDISSINFKELSSEDVRRYHFVDVAVAFTFYNWYASCHGFAARKSKVMRSKSGELTQQTLVCYRQGFTEDLSCLLWWIYYFYI*